Long-term Increase Research Articles

Inflammation-induced mast cell-derived nerve growth factor: a key player in chronic vulvar pain?

Provoked vulvodynia (PV) is characterized by localized chronic vulvar pain. It is associated with a history of recurrent inflammation, mast cell (MC) accumulation, and neuronal sprouting in the vulva. However, the mechanism of how vulvar-inflammation promotes neuronal sprouting and gene-expression adaptation in the spinal cord, leading to hypersensitivity and painful sensations, is unknown. Here, we found that vulvar tissue from women with PV (n=8) is characterized by MC accumulation and neuronal sprouting compared to women without PV (n=4). In addition, we observed these changes in an animal study of PV. Thus, we found that repeated vulvar zymosan-inflammation challenges lead to long-lasting mechanical and thermal vulvar hypersensitivity, which was mediated by MC accumulation, neuronal sprouting, overexpression of the pain channels (TRPV1 and TRPA1) in vulvar neurons, as well as a long-term increase of gene expression related to neuroplasticity, neuroinflammation, and nerve growth factor (NGF) in the spinal cord/DRG(L6-S3). However, regulation of the NGF pathway by stabilization of MC activity with ketotifen fumarate (KF) during vulvar inflammation attenuated the local increase of NGF and histamine, as well as the elevated transcription of pro-inflammatory cytokines, and NGF pathway in the spinal cord. Additionally, KF treatment during inflammation modulates MC accumulation, neuronal hyperinnervation, and overexpression of the TRPV1 and TRPA1 channels in the vulvar neurons, consequently preventing the development of vulvar pain. A thorough examination of the NGF pathway during inflammation revealed that blocking NGF activity by using an NGF-non-peptide-inhibitor (Ro08-2750) regulates the upregulation of genes related to neuroplasticity, and NGF pathway in the spinal cord, as well as modulates neuronal sprouting and overexpression of the pain channels, resulting in a reduced level of vulvar hypersensitivity. On the other hand, stimulation of the NGF pathway in the vulvar promotes neuronal sprouting, overexpression of pain channels, and increase of gene expression related to neuroplasticity, neuroinflammation, and NGF in the spinal cord, resulting in long-lasting vulvar hypersensitivity. In conclusion, our findings suggest that vulvar allodynia induced by inflammation is mediated by MC accumulation, neuronal sprouting, and neuromodulation in the vulvar. Additionally, chronic vulvar pain may involve a long-term adaptation in gene expression in the spinal cord, which probably plays a critical role in central sensitization and pain maintenance. Strikingly, regulating the NGF pathway during the critical period of inflammation prevents vulvar pain development via modulating the neuronal changes in the vestibule and spinal cord, suggesting a fundamental role for the NGF pathway in PV development.

Morphodynamics of the dredged channel in a mega fluvial-tidal delta

Deltaic channels are key pathways connecting rivers and the ocean, which is of significance for in maintaining estuarine stability and material transport processes between land and sea. However, deltaic channels have experienced dramatic variations in the Anthropocene era, including seriously scouring induced by decline of riverine sediment delivered to the ocean, trenching caused by dredged works and episodic filling by storm events. Here, a series of hydrology, sediment as well as elevation data between 1979 and 2020 of North Passage (NP), one of most important navigated channels over the Changjiang Estuary Delta, were used to analyze its long-term morphodynamic processes. Results show that during 1979–2020, NP capacity presented stage changes: gentle increase stage between 1979 and 1997, fluctuation stage between 1997 and 2002, fast-descend stage between 2002 and 2007, slow growth stage between 2007 and 2020, and periodic signals of 13 and 25 months can be detected in NP volume. Meanwhile, the shoal zone and the deep channel showed long-term decrease and increase trend respectively, making NP turn into a narrow and deep deltaic channel. Moreover, NP exhibited two major morphodynamic patterns. The first mode indicates the long-term continuous erosion of the main-channel region and persistent deposition in the groin region. The second mode explains the transition of the erosion/deposition state of NP, where the huge mouth bar system in NP was shifted seaward accompanied by a decrease of its extent. The periodic changes of fluvial water and sediment may contribute the oscillation of erosion and accretion process of NP. The construction of engineering structures and long-term dredging works resulted in constant deposition in the groin region with continuous erosion in main-channel region. The existence of estuarine turbidity maximum was conductive to deposition in the main-channel region.

Serotonergic psychedelic 5-MeO-DMT alters plasticity-related gene expression and generates anxiolytic effects in stressed mice.

Serotonergic psychedelics have potential therapeutic effects in treating anxiety and mood disorders, often after a single dose, and are suggested to have plasticity-inducing action. However, a comprehensive mechanism of action is still lacking. Here, we investigated how a single dose of the short-acting 5-methoxy-N,N-dimethyltryptamine (5-MeO-DMT) acts on gene expression from microdissected brain regions (anterior cingulate cortex - ACC; basolateral amygdala - BLA; ventral hippocampus CA1 region - vCA1 and dentate gyrus-DG) of naive and stressed mice. Specifically, we compared gene expression of Arc, Zif268, BDNF, CREB, mTORC1, NR2A, TRIP8b, and NFkB in mice injected with 5-MeO-DMT or saline at different time points (1 h, 5 h, or 5 days prior). 5-MeO-DMT altered mRNA expression of immediate early genes Arc and ZiF268 in the ACC, BLA, and vCA1, while NR2A expression was decreased after 5 h in the vCA1. We also found a long-term increase in TRIP8b, a gene related to the modulation of neuronal activity, in the vCA1 after 5 days. Behaviorally, 5-MeO-DMT treated mice showed mixed anxiolytic and anxiogenic effects in the elevated plus maze and open field test 24 h or 5 days after treatment. However, pre-treated mice subjected to acute stress showed both lower corticosterone levels and robust anxiolytic effects of 5-MeO-DMT administration. Together, our findings provide insights into the molecular actions of 5-MeO-DMT in the brain related to anxiolytic effects of behavior.

Prolonged increase in psychotropic drug use among young women following the COVID-19 pandemic: a French nationwide retrospective study

BackgroundThe COVID-19 pandemic has had a significant impact on mental health, with evidence suggesting an enduring mental health crisis. Studies worldwide observed increased usage of antidepressants, anxiolytics, and hypnotics during the pandemic, notably among young people and women. However, few studies tracked consumption post-2021. Our study aimed to fill this gap by investigating whether the surge in the number psychotropic drug consumers in France persisted 2 years after the first lockdown, particularly focusing on age and gender differences.MethodsWe conducted a national retrospective observational study based on the French national insurance database. We retrieved all prescriptions of anxiolytics, hypnotics, and antidepressants dispensed in pharmacies in France for the period 2015–2022. We performed interrupted time series analyses based on Poisson models for five age classes (12–18; 19–25; 26–50; 51–75; 76 and more) to assess the trend before lockdown, the gap induced and the change in trend after.ResultsIn the overall population, the number of consumers remained constant for antidepressants while it decreased for anxiolytics and hypnotics. Despite this global trend, a long-term increase was observed in the 12–18 and 19–25 groups for the three drug classes. Moreover, for these age classes, the increases were more pronounced for women than men, except for hypnotics where the trends were similar.ConclusionsThe number of people using antidepressants continues to increase more than 2 years after the first lockdown, showing a prolonged effect on mental health. This effect is particularly striking among adolescents and young adults confirming the devastating long-term impact of the pandemic on their mental health.

First Data Related to Evaluation of Bacterial Diversity and Physicochemical Structure for Winter in Kilis Surface Waters, Türkiye

Water temperature is one of mainly the seasonal dynamics influencing microbial quality in aquatic ecosystems; however, discerning the interaction betweeen temperature and bacterial diversity is challenging. To address this issue, we characterized the microbial community of surface waters obtained from November month (in 1990 and 2020) with of average 1 °C temperature increase at the Kilis, Türkiye by the 16S rRNA gene sequencing. Bacterial communities indicated high similarities in the relative abundances of dominant taxa (Proteobacteria) for both sampling sites. Our findings highlighted that temperature change favored the duplication of opportunistic thermotolerant bacteria, including total coliform and E.coli which may increase waterborne-disease. This study provides comprehensive information about phylogenetic diversity, quantitatively assessing indicator microorganisms and the physicochemical potential of water quality within two surface waters in Kilis. Our climatic data estimates to be the November average rate of 2.5 °C/increase associated with air temperature of Kilis region for next 30 years. The current study is the first exploration to combine climate change with molecular approaches to predict how bacterial concentrations in aquatic environments may change in future based on long-term air temperature increase for Kilis region.

Sustained increases in atmospheric oxygen and marine productivity in the Neoproterozoic and Palaeozoic eras

A geologically rapid Neoproterozoic oxygenation event is commonly linked to the appearance of marine animal groups in the fossil record. However, there is still debate about what evidence from the sedimentary geochemical record—if any—provides strong support for a persistent shift in surface oxygen immediately preceding the rise of animals. We present statistical learning analyses of a large dataset of geochemical data and associated geological context from the Neoproterozoic and Palaeozoic sedimentary record and then use Earth system modelling to link trends in redox-sensitive trace metal and organic carbon concentrations to the oxygenation of Earth’s oceans and atmosphere. We do not find evidence for the wholesale oxygenation of Earth’s oceans in the late Neoproterozoic era. We do, however, reconstruct a moderate long-term increase in atmospheric oxygen and marine productivity. These changes to the Earth system would have increased dissolved oxygen and food supply in shallow-water habitats during the broad interval of geologic time in which the major animal groups first radiated. This approach provides some of the most direct evidence for potential physiological drivers of the Cambrian radiation, while highlighting the importance of later Palaeozoic oxygenation in the evolution of the modern Earth system.

Liposuction as a Treatment for Lipedema: A Scoping Review.

Lipedema is the progressive symmetrical deposition of subcutaneous fat and fluid in the lower body, ordinarily sparing the trunk, upper limbs, face, and neck. It may follow an autosomal dominant inheritance pattern. The gold standard treatment for lipedema is complete decongestive therapy, consisting of manual lymphatic drainage and compression garments. This scoping review assessed the existing literature on the effectiveness of liposuction as an alternative treatment for lipedema. A scoping review of electronically available literature within PubMed, Scopus, and Cochrane focused on liposuction as a treatment for lipedema considering the following inclusion criteria: human studies, case series of 10 or more, controlled trials, randomized controlled trials, patient-reported outcome measurement studies, survey analyses, descriptive studies, retrospective analyses, recurrence included, follow-up of 6 months or more, age 18 years or older, and treatment modality being liposuction. Thirteen studies were selected. Nine studies reported decreased compression therapy use among patients following liposuction. No studies reported a long-term increase in compression therapy following liposuction. Studies found self-reported improvements in pain, mobility, bruising, and overall quality of life for patients following liposuction, many of whom had previously been on compressive therapy. Studies reported low rates of serious adverse events following liposuction, including deep vein thrombosis, pulmonary embolism, and infection. These results suggest that liposuction can be a viable treatment alternative to compression therapy for lipedema in patients whose compression therapy has not been helpful. However, there is not enough evidence to say whether liposuction is as effective as compression for patients first presenting with lipedema.

Impact of the Sewol Ferry Disaster on Physical Changes: Focused on BMI

This study aims to empirically verify whether disasters affect physical changes, linking research on the effects of disasters on psychology and the effects of psychology on physical changes. It focused on the Sewol ferry disaster, and physical changes were tracked from 2010 to 2018, including the four years before and after the disaster in 2014. We constructed panel data on obesity rates from 229 local governments and used longitudinal data to analyze the impact of large disasters on changes in obesity rates over time. The results indicated that the Sewol Ferry Disaster had a long-term effect on the increase in obesity rate over time. In the directly affected areas of Ansan and Jindo, there was not only a long-term increase but also an immediate increase in the obesity rate. The findings of this study provide an opportunity to expand our understanding of the impact of disasters on society, and can also serve as foundational data for national and local disaster-related long-term impact support policies.

The regionality and seasonality of tornado trends in the United States

Continued efforts to build human resilience to the impacts of tornadoes require updated knowledge of tornado occurrences as well as how their occurrence characteristics may be changing in time and varying by region. We have temporally and geospatially disaggregated annual tornado reports in the United States and revealed that significant, long-term decreases in tornado days from 1960 to 2022 have occurred over the months of June through August, primarily within the Southern Great Plains. In contrast, long-term increases in days of tornado outbreaks have occurred over this period, particularly within the Southeast U.S. and during warm- as well as cool-season months. There are indications that these dichotomous linear trends in tornado days and tornado outbreaks have relaxed over the most recent decade. Our study highlights the need to better understand the role of internal climate variability and anthropogenic forcing in modulating tornado activity.

Effects of belimumab on the lipid profile in systemic lupus erythematosus patients: an observational study.

This study is asked to investigate the effects of belimumab on the lipid profile in systemic lupus erythematosus (SLE) patients. Forty-one SLE patients who received at least 6months of belimumab treatment were retrospectively analyzed. The control group consisted of 56 age- and sex-matched lupus patients not treated with belimumab. The changes in lipid profile after a 6-month treatment were compared between the two groups. Generalized estimating equation (GEE) analyses were performed to examine lipid levels longitudinally during the period and the effect of clinical response variables and medication on the lipid profile in the belimumab group. In the belimumab group, high-density lipoprotein (HDL) cholesterol levels increased significantly after the 6-month treatment (P = 0.02). After 1month, HDL, apolipoprotein A-I (apoA-I) significantly increased by 13.8 and 11.4%, compared with baseline, respectively. After 3months, HDL and apoA-I increased by 9.0 and 7.1%, respectively. After 6months, HDL increased by 7.6% compared with baseline. Total cholesterol, triglycerides, low-density lipoprotein cholesterol, and apolipoprotein B did not change significantly over the course of treatment. GEE analyses indicated a significant association between HDL and disease activity indexes, such as IgG, anti-dsDNA, and complement C3. Subgroup analysis revealed significant changes in HDL only in patients who had achieved a ≥ 4-point reduction in SLEDAI-2K after 6months of belimumab treatment. Belimumab treatment may result in a long-term increase in HDL level in SLE patients by improving control of lupus activity. This might have beneficial effects on controlling cardiovascular risk in lupus patients. Key Points • Treatment with belimumab resulted in a significant and sustained increase in the HDL levels in SLE patients. • Significant changes in HDL were observed in lupus patients treated with belimumab who had a better clinical response.

The long-term effect of bariatric/metabolic surgery versus pharmacologic therapy in type 2 diabetes mellitus patients: A systematic review and meta-analysis.

Metabolic/bariatric surgery as a treatment for obesity and related diseases, such as type 2 diabetes mellitus (T2DM), has been increasingly recognised in recent years. However, compared with conventional pharmacologic therapy, the long-term effect (≥ 5years) of metabolic surgery in T2DM patients is still unclear. This study aimed to evaluate the diabetes remission rate, incidence of diabetic microvascular complications, incidence of macrovascular complications, and mortality in T2DM patients who received metabolic surgery versus pharmacologic therapy more than 5years after the surgery. Searching the database, including PubMed, Embase, Web of Science, and Cochrane Library from the inception to recent (2024), for randomised clinical trials (RCTs) or cohort studies comparing T2DM patients treated with metabolic surgery versus pharmacologic therapy reporting on the outcomes of the diabetes remission rate, diabetic microvascular complications, macrovascular complications, or mortality over 5years or more. A total of 15 articles with a total of 85,473 patients with T2DM were eligible for review and meta-analysis in this study. There is a significant long-term increase in diabetes remission for metabolic surgery compared with conventional medical therapy in the overall pooled estimation and RCT studies or cohort studies separately (overall: OR=4.58, 95% CI: 1.89-11.07, P<0.001). Significant long-term decreases were found in the pooled results of microvascular complications incidence (HR=0.57, 95% CI: 0.41-0.78, P<0.001), macrovascular complications incidence (HR=0.59, 95% CI: 0.50-0.70, P<0.001) and mortality (HR=0.53, 95% CI: 0.53-0.79, P=0.0018). Metabolic surgery showed more significant long-term effects than pharmacologic therapy on diabetes remission, macrovascular complications, microvascular complications incidence, and all-cause mortality in patients with T2DM using currently available evidence. More high-quality evidence is needed to validate the long-term effects of metabolic surgery versus conventional treatment in diabetes management.

Sustainable bacteria-based self-healing steel fiber reinforced concrete

Self-healing concrete presents an innovative and eco-friendly solution with the potential to reduce maintenance costs through self-activated crack repair. This study examined the effect of varying bacterial content across different environmental conditions. Additionally, the coupling effect between bacteria and steel fiber to enhance the mechanical and physical properties at different ages has been investigated. Bacillus Sphaericus (B. Sphaericus) was incorporated into the concrete at concentrations of 2 × 109 (CFU/ml), and different bacterial percentages selected utilizing calcium lactate as a nutrient source. Bacterial content of 0.0 %, 0.25 %, 1.0 %, and 2.5 %, cured in both fresh water and magnesium sulfate water solution, were tested to examine concrete behavior in harsh environments. Additionally, steel fibers at volume fraction percentages (Vf%) of 1.0 % and 1.5 %, along with 1.0 % bacterial content, were tested to highlight their combined effect. Tests were conducted at different ages to measure compressive, tensile, and flexural strengths. In addition, SEM and EDS analyses for cracked and uncracked specimens were performed. The results showed a significant increase in the long-term compressive, tensile, and flexural strengths at 180 days by 47 %, 80 %, and 50 %, respectively, with a bacterial content of 2.5 %. Moreover, the incorporation of steel fibers, 1.0 % and 1.5 % content, in the bacterial concrete resulted in a long-term compressive strength increase at 180 days by 45 % and 55 %, respectively with 1.0 % bacterial content, compared to 15 % and 27 % for 0 % bacteria. Curing in sulfate-rich water, concrete specimens exhibited increasing compressive strength at 180 days from 47 MPa for 0 % bacteria to 77 MPa with 2.5 % bacteria content. Despite lower initial strength of 29 MPa at 7 days, bacterial incorporation led to significant strength improvements, highlighting effective self-healing even in aggressive curing environments.

High-quality research on physical therapy in psoriatic arthritis is needed: a systematic review.

Although physical therapy is recommended as part of the non-pharmacological management of patients with psoriatic arthritis (PsA), the evidence is still unclear. Therefore, this study aimed to systematically review and appraise the quality of research on physical therapy in the management of patients with PsA. In June 2024, a systematic literature search using four different databases (Medline, Embase, Web of Science and the Cochrane Library) was performed to include interventional and observational studies examining physical therapy in patients with PsA (PROSPERO ID 255501). A risk of bias assessment was conducted. Due to the wide variety of interventions and outcomes, a narrative synthesis was used. Of 9442 abstracts, 15 papers examining physical therapy uptake in clinical practice (N = 2) and different physical therapy interventions (N = 13) were included: cardiorespiratory exercises (N = 5), resistance exercises (N = 2), therapeutic modalities (N = 4) and mixed rehabilitation programs (N = 2). A low risk of bias was scored in only one RCT assessing cardiorespiratory exercises. The well-tolerated 11-week high-intensity interval training resulted in a long-term increase in peak oxygen uptake and a short-term decrease in truncal fat percentage in patients with low disease activity. Resistance training in patients with active disease did not increase muscle strength, but improved functional capacity, disease activity, pain and general health after the intervention. Evidence for other modalities was inconclusive. High-quality evidence on physical therapy in PsA was scarce. Cardiorespiratory and resistance exercises demonstrated promising results to positively influence cardiometabolic risk as well as disease-related outcomes. Future research on physical therapy in PsA with adequate methodological quality is needed.

The COVID-19 pandemic and the use of benzodiazepines and benzodiazepine-related drugs in Estonia: an interrupted time-series analysis

BackgroundThe COVID-19 pandemic has posed challenges that worsened people’s mental health. We explored the impact of the COVID-19 pandemic on the mental well-being of the population, as indicated by the prevalence rates of benzodiazepine and benzodiazepine-related drug (BDZ) use.MethodsThis population‐based, time‐series analysis included all prescriptions of BDZs dispensed in Estonia between 2012 and 2021. The monthly prevalence rates of BDZ use were calculated. Autoregressive integrated moving average models with pulse and slope intervention functions tested for temporary and long-term changes in monthly prevalence rates after the onset of the COVID-19 pandemic.ResultsThroughout the 10-year study period, a total of 5,528,911 BDZ prescriptions were dispensed to 397,436 individuals. A significant temporary increase in the overall prevalence rate of BDZ use in March 2020 (2.698 users per 1000, 95% CI 1.408–3.988) was observed, but there was no statistically significant long-term change. This temporary increase affected all the examined subgroups, except for new users, individuals aged 15–29 years, and prescribing specialists other than general practitioners and psychiatrists. The long-term increase in BDZ use was confined to females aged 15–29 years (0.056 users per 1000 per month, 95% CI 0.033–0.079), while no significant change was observed among males of the same age (0.009 users per 1000 per month, 95% CI – 0.017 to 0.035). Among females aged 15–29 years, a significant long-term increase in BDZ use was observed for anxiety disorders (0.017 users per 1000 per month, 95% CI 0.010–0.023), depressive disorders (0.021 users per 1000 per month, 95% CI 0.012–0.030), and other mental and behavioral disorders (0.020 users per 1000 per month, 95% CI 0.010–0.030), but not for sleep disorders (– 0.008 users per 1000 per month, 95% CI – 0.018–0.002).ConclusionThe COVID-19 pandemic led to a short-term increase in BDZ use immediately after the pandemic was declared. In the long term, young females experienced a sustained increase in BDZ use. The prolonged effect on girls and young women suggests their greater vulnerability. These results underscore the need to effectively address the long-term effects of the pandemic among youth.

Life-history updates and stock assessment of Macrodon atricauda (teleostei, sciaenidae) from southern Brazil

The coastal demersal sciaenid fish Macrodon atricauda has been intensely fished since the 1950s along the southern Brazilian coast, resulting not only in decreasing abundance but also in long-term changes in its life history. Previous studies reported long-term increases in individual growth, size, and age at maturity. Using individual observed length-at-age, we did not observe the previously reported growth changes despite the increase in the mean length of older individuals from 1976 to 2023. These observed changes did not affect the estimation of the von Bertalanffy growth parameters, likely due to the species' faster growth at earlier ages and short lifespan. The length at first maturity remains smaller than the previous estimations from the 1970s but has not changed since the 2000s. The population dynamics assessed considering the updated life history parameter, including the size at maturity changes, showed that the species is not suffering from overfishing (i.e., F2019/FMSY = 0.17). However, the spawning stock biomass was estimated at low levels (i.e., SSB2019/SSBMSY = 0.59), indicating an overfished status highlighting the need for management action. The apparent stability of the life-history parameters observed for the species in the last four decades may be attributed to a combination of traits promoting resilience and recovery, including high fecundity, low age at maturity, fast individual growth, and a relatively short lifespan. In fact, despite decades of intense fishing, M. atricauda remains an important coastal demersal species in the landings of southern Brazil.

Multi-scale characterisation of cold response reveals immediate and long-term impacts on cell physiology up to seed composition in sunflower.

Early sowing can help summer crops escape drought and can mitigate the impacts of climate change on them. However, it exposes them to cold stress during initial developmental stages, which has both immediate and long-term effects on development and physiology. To understand how early night-chilling stress impacts plant development and yield, we studied the reference sunflower line XRQ under controlled, semi-controlled and field conditions. We performed high-throughput imaging of the whole plant parts and obtained physiological and transcriptomic data from leaves, hypocotyls and roots. We observed morphological reductions in early stages under field and controlled conditions, with a decrease in root development, an increase in reactive oxygen species content in leaves and changes in lipid composition in hypocotyls. A long-term increase in leaf chlorophyll suggests a stress memory mechanism that was supported by transcriptomic induction of histone coding genes. We highlighted DEGs related to cold acclimation such as chaperone, heat shock and late embryogenesis abundant proteins. We identified genes in hypocotyls involved in lipid, cutin, suberin and phenylalanine ammonia lyase biosynthesis and ROS scavenging. This comprehensive study describes new phenotyping methods and candidate genes to understand phenotypic plasticity better in response to chilling and study stress memory in sunflower.

Variability of hydrographic and biogeochemical properties in the North-western Adriatic coastal waters in relation to river discharge and climate changes

The dynamics of hydrographic and biogeochemical properties in a Northwestern coastal area of the Adriatic Sea were investigated. The time series data from continuous observation (2007–2022) allowed the investigation of annual trends and seasonal cycles along a coastal transect influenced by local river discharge. Various statistical models were used to investigate water temperature, salinity, chlorophyll a, dissolved organic, inorganic and particulate nutrients, precipitation and river discharge. It was found that the local river discharge regime played an essential role in interannual, and seasonal biogeochemical dynamics associated with global climate change in the Mediterranean region. A significant trend towards oligotrophic conditions was detected, as evidenced by the downward trend in the river mouth and on the sea of chlorophyll a (−0.2 μg L−1 in the sea), dissolved organic and inorganic nitrogen and phosphorus (i.e., −0.43 μM yr−1 of DON in the sea and −6.67 of DIN μM yr−1 in the river mouth or −0.07 μM yr−1 of DOP and −0.02 μM yr−1 of DIP in the river mouth) and silicate (−2.47 μM yr−1 in the river mouth) concentrations. Salinity showed a long-term increase in the sea (0.08 yr−1), corresponding to a significant decrease in water discharge from the local river (−0.27 m3 s−1 yr−1) and precipitation (−0.06 mm yr−1). The dissolved organic and inorganic nutrients highlighted a different seasonal accumulation under the river runoff regime. The nutrient enrichment was predominantly driven by river contribution. Data analysis showed that the coastal biogeochemical properties dynamics were mostly influenced by river discharge and precipitation regimes, which in turn are driven by climate change variability in the North-western Adriatic Sea.

Understanding the role of the spatial-temporal variability of catchment water storage capacity and its runoff response using deep learning networks

The land surface of a watershed acts as a large reservoir, with its catchment water storage capacity (CWSC) influencing rainfall-runoff relationship. Estimating CWSC at global grid scale is challenging due to calibration complexity, limited spatial continuity, and data scarcity. To address this, a deep learning-based approach incorporates spatial reconstruction and temporal transfer for capturing spatio-temporal variations in watershed characteristics. The study focuses on the Global Runoff Data Centre dataset and presents a grid-based hydrological model. Findings demonstrate accurate identification of CWSC distribution, with the model achieving an R 2 of 0.92 and the runoff Kling–Gupta efficiency of 0.71 during validation. According to the CMIP6 projections, the global CWSC is anticipated to undergo a significant increase at a rate of 1.7 mm per decade under the SSP5-8.5 emission scenario. Neglecting spatio-temporal CWSC variability globally overestimates climate change’s impact on runoff, potentially reducing the projected long-term increase by up to 41%.

#1665 Mechanisms underlying long-term weight changes and their impact on all-cause mortality among chronic hemodialysis patients

Abstract Background and Aims Observational studies have demonstrated a significant association between long-term weight loss and weight gain and an increased risk of mortality among chronic hemodialysis patients. However, underlying mechanisms of these associations remain insufficiently explored. To address this knowledge gap, we conducted an in-depth analysis using a cohort of hemodialysis patients, benefiting from comprehensive baseline data, regular six-month evaluations, and an extended follow-up period. We employed advanced statistical techniques, including Lasso regression, to construct predictive models for long-term weight loss and gain. Furthermore, we conducted mediation analyses to elucidate whether weight change mediates the impact of major risk factors on all-cause mortality. Method This post-hoc analysis utilized data from participants in a previous randomized controlled trial conducted between 2006 and 2011, named as the Olmesartan clinical trial in Okinawan patients under the OKIDS (OCTOPUS) study. Subsequently, we tracked participants' mortality outcomes until July 2018. At the time of enrollment, we collected comprehensive baseline information, encompassing demographics, medical history, and laboratory data. Throughout the trial, participants' body weights were meticulously recorded every six months. Our analysis was restricted to patients who survived for at least two years post-enrollment, ensuring a minimum of five body weight measurements during the trial. Lasso regression was employed in training datasets (comprising 60% of participants) to identify crucial risk factors associated with long-term weight gain (&amp;gt;1 kg per year) or weight loss (&amp;lt;−1 kg per year) among chronic hemodialysis patients. These selected models were subsequently applied to paired validation datasets (comprising 40% of participants), and receiver operating characteristic (ROC) curves were used to calculate the area under the curve (AUC), aiding in the identification of key risk factors for long-term weight changes. Subsequently, we conducted mediation analyses to investigate whether weight change mediated the relationship between risk factors and all-cause mortality. Results Our analysis included 404 out of 461 OCTOPUS participants, with 60.9% being male, a mean age of 59.2 years (±11.7), and a mortality rate of 41.6%. The median follow-up period extended to 10.3 years. In the training datasets, Lasso models identified critical predictors for weight increase, including a history of diabetes, younger age, normal cardio-thoracic ratio (CTR) (&amp;lt;50% for males and &amp;lt;53% for females), and BMI &amp;lt;20. Conversely, predictors for weight decrease included BMI ≥20, serum sodium &amp;lt;135 mEq/L, serum albumin &amp;lt;3.5 g/dL, and older age. Weight increase demonstrated higher predictability (mean AUC of 0.59) compared to weight decrease (mean AUC of 0.55) in the validation datasets. Mediation analyses revealed that weight loss mediated 30% of the impact of older age (≥70 years old) on all-cause mortality (P = 0.068). Conclusion Our findings suggest that, among chronic hemodialysis patients, long-term weight increase is associated with a history of diabetes and younger age, while long-term weight loss is linked to BMI (≥20), hyponatremia, hypoalbuminemia, and older age. Notably, long-term weight loss appears to explain 30% of the increased risk of all-cause mortality associated with older age (≥70 years old).

EXPLORATION AND COMPARISON BETWEEN REGULAR CONCRETE AND CONCRETE WITH MINERAL ADDITIVES

Concrete is a fundamental building material with critical properties such as durability, strength, and versatility. In recent years, there has been increasing interest in enhancing concrete performance and sustainability by incorporating mineral additives such as fly ash, slag, and silica fume. This study explores and compares the characteristics of regular concrete and concrete with mineral additives to evaluate their suitability for various construction applications. The research focuses on the mechanical properties, durability, and environmental impacts of both types of concrete. Laboratory tests will be conducted to compare the compressive strength, tensile strength, resistance to freeze-thaw cycles, chemical attacks, and workability of regular concrete and concrete with mineral additives. Additionally, life cycle assessments will be performed to analyze the environmental implications of using mineral additives in concrete production. One of the main advantages of mineral additives is their potential to improve the strength and durability of concrete. Fly ash, slag, and silica fume have been shown to enhance long-term strength, reduce permeability, and increase resistance to chemical attacks in concrete mixtures. Furthermore, using mineral additives can reduce the reliance on cement, a primary component of concrete with a significant carbon footprint, leading to reduced greenhouse gas emissions and resource depletion. Index Terms – Concrete, Mineral additives, Durability, Strength.