Long-term Studies Research Articles

Evaluation of the therapeutic effects of nebulized inhalation of hydrogen-rich water on primary blast lung injury in C57BL/6 mice.

Primary blast lung injury is a common and severe consequence of explosion events, characterized by immediate and delayed effects such as apnea and rapid shallow breathing. The overpressure generated by blasts leads to alveolar and capillary damage, resulting in ventilation-perfusion mismatch and increased intrapulmonary shunting. This reduces the effective gas exchange area, causing hypoxemia and hypercapnia. Hydrogen (H2), a small-molecular-weight, nonpolar diatomic molecule, has shown potential in treating various diseases due to its antioxidant and anti-inflammatory properties. This study evaluates the therapeutic effects of nebulized hydrogen-rich water on primary blast lung injury in C57BL/6 mice and explores the underlying mechanisms. C57BL/6 mice (n= 150), aged 6-8weeks, were randomly divided into 2 groups: the blast injury control group (n= 75) and the nebulized hydrogen-rich water treatment group (n= 75). Mice were exposed to a blast overpressure of 266±9.156 kPA and treated with either nebulized hydrogen-rich water or sterile injection water immediately postinjury. Observations were made at 6, 12, 24, and 48hours postinjury. Lung function was assessed using whole-body plethysmography, and arterial blood gases were analyzed. Lung tissue was examined histologically and biochemically for markers of inflammation and oxidative stress. The survival rates at different time points postinjury in the nebulized hydrogen-rich water treatment group were significantly higher than those in the blast injury control group (6hours postinjury: 89.3% vs 78.6%; 12hours postinjury: 81.3% vs 72%; 24hours postinjury: 81.3% vs 61.3%, P < .01). Lung function tests revealed significant improvements in tidal volume (12hours postinjury: 0.11±0.018 vs 0.08±0.016, 24hours postinjury: 0.16±0.013 vs 0.12±0.013, 48hours postinjury: 0.18±0.02 vs 0.13±0.014), respiratory rate (6hours postinjury: 235.07±12.82 vs 268.29±13.73; 12hours postinjury: 265.47±10.06 vs 342.16±16.34; 24hours postinjury: 248.20±9.28 vs 352.80±15.99; 48hours postinjury: 226.12±15.81 vs 318.18±15.81), and minute ventilation (12hours postinjury: 22.05±3.46 vs 15.93±3.68; 24hours postinjury: 27.30±2.15 vs 21.62±2.48; 48hours postinjury: 37.48±3.93 vs 28.32±2.98) in the nebulized hydrogen-rich water treatment group (P < .01). Arterial blood gas analysis indicated better oxygenation and reduced hypercapnia in the nebulized hydrogen-rich water treatment group (P < .05). Histologic examination showed reduced lung edema and hemorrhage in the nebulized hydrogen-rich water treatment group. Levels of inflammatory cytokines (interleukin-1β, interleukin-6, and tumor necrosis factor α) and oxidative stress markers (ie, malondialdehyde) were significantly lower, whereas antioxidant enzyme (total superoxide dismutase) activity was higher in the nebulized hydrogen-rich water treatment group compared with the blast injury control group (P < .01). Arterial blood gas analysis indicated better oxygenation and reduced hypercapnia in the nebulized hydrogen-rich water treatment group (P < .05). Histologic examination showed reduced lung edema and hemorrhage in the nebulized hydrogen-rich water treatment group. Levels of inflammatory cytokines (interleukin-1β, interleukin-6, and tumor necrosis factor α) and oxidative stress markers (ie, malondialdehyde) were significantly lower, whereas antioxidant enzyme (total superoxide dismutase) activity was higher in the nebulized hydrogen-rich water treatment group compared with the blast injury control group (P < .01). Hydrogen-rich water treatment significantly improves survival rates and lung function and reduces inflammation and oxidative stress in mice with primary blast lung injury. These findings suggest that hydrogen's antioxidant and anti-inflammatory properties play a crucial role in mitigating lung damage and improving respiratory function postinjury. Further long-term studies and imaging analyses are needed to confirm these findings and elucidate the molecular mechanisms involved. This study provides a theoretical basis for the clinical application of hydrogen-rich water in treating blast-induced lung injuries.

Phosphorus Dynamics in Managed and Natural Soils: SEM-PLS Analysis of Vaccinium, Forest, and Grassland Ecosystems.

Phosphorus (P) availability in soils is often constrained by its accumulation in non-labile phosphorus (NLP) forms, limiting its accessibility to plants. This study examines how soil physical properties, chemical characteristics, and climatic conditions influence phosphorus fractionation and the transformation of NLP into plant-available labile phosphorus (LP). Utilizing global structural equation modeling (SEM), we found that silt content enhances organic phosphorus fractions, including NaHCO3-Po and NaOH-Po. In the upper 30 cm of soil, pH decreases the availability of NaHCO3-Po and NaOH-Po while stabilizing NLP, highlighting its essential role in phosphorus cycling under acidic conditions. In deeper soil layers, pH facilitates phosphorus mobilization from NLP pools, with effects varying across fractions. Long-term studies on Japanese Vaccinium soils reveal that pH and electrical conductivity (EC) management significantly promote NLP-to-LP conversion, primarily through NaOH-Po, thereby improving phosphorus use efficiency. These findings underscore the critical importance of prioritizing chemical property management over physical modifications to optimize nutrient cycling, preserve soil fertility, and reduce reliance on external phosphorus inputs in agricultural systems. Our study emphasizes the need for integrated approaches to achieve sustainable phosphorus management in both natural and managed ecosystems.

Drug-coated balloons across different scenarios. Long-term single center experience

Drug-coated balloons across different scenarios. Long-term single center experience

Long-term Prospective Comparative Analysis of Ototoxic and Survival Outcomes of Sequential Boost and Simultaneous Integrated Boost of Volumetric Modulated Arc Therapy for Head-Neck Carcinomas.

To compare the ototoxicity and survival in head and neck carcinoma patients treated with sequential (SEQ) and simultaneous integrated boost (SIB) of volumetric modulated arc therapy (VMAT). This long-term prospective study enrolled patients with histologically confirmed head and neck carcinoma, all receiving VMAT treatment. Audiological assessments were done using various tests at baseline, two weeks, treatment completion, six months, and 12 months. The changes in bone conduction pure tone thresholds were correlated with cochlear dose, comparing SEQ and SIB plans. We also investigated other significant late toxicities that led to dysphagia, voice changes, and xerostomia. Survival was assessed with the Kaplan-Meier analysis. The study included 93 patients (186 ears), 40 receiving radiation alone and 53 undergoing chemoradiation. Baseline hearing levels for the right and left ears were 13.3±2.3 dB and 14.2±1.5 dB. After 12 months of radiation, levels were 18.5±2.4 dB and 19.11±1.9 dB, respectively. No significant changes were observed between SEQ and SIB plans, but high-frequency shifts occurred. The cochlea tolerated up to 28 Gy without hearing loss in the radiation-alone group but showed loss at 9 Gy when combined with cisplatin chemotherapy. The maximum dose (Dmax) and the mean dose (Dmean) of pharyngeal constrictor muscles predicted dysphagia. No significant SEQ vs. SIB differences were found in late toxicity or survival outcomes. Modern radiotherapy techniques like VMAT adhere to cochlear dose limits. No significant differences were found between SEQ and SIB plans in sensorineural hearing loss, late toxicity, or survival, making both suitable for head and neck carcinoma treatment.

Therapy for Dupuytren's Disease (II): Collagenase Therapy vs. Limited Fasciectomy-A Long-Term Comparative Study.

Dupuytren's disease (DD) is a systemic connective tissue disorder of the palm, predominantly affecting men of Northern European or Caucasian origin over 55. In addition to conventional surgery, Dupuytren's contracture can be treated in a minimally invasive way by injecting bacterial collagenase into the cord. However, studies on the long-term success rate when compared to the gold standard, surgical limited fasciectomy, are limited. This monocentric retrospective study examined 35 patients who had been treated with bacterial collagenase for Dupuytren's contracture, conducting a long-term follow-up after an average of 5.7 years. The results were compared to a control group of 40 patients treated with surgical limited fasciectomy on average 5.5 years ago. Finger extension (Tubiana stage), strength, sensitivity, the effect of possible risk factors, and patient-reported outcome measures (PROMs) were compared between the two groups. The long-term results after therapy for DD showed a significant reduction in the Tubiana stage for both groups (p < 0.001). Additionally, we observed a longer mean preintervention Tubiana stage and a better long-term improvement in the Tubiana stage for patients with limited fasciectomy when compared to the collagenase group. (both p < 0.001). Neither grip strength nor the pinch test showed significant differences when compared within each group or when comparing both groups. Both the treated and untreated fingers of patients with limited fasciectomy had a superior two-point discrimination (p < 0.001). For the URAM questionnaire, we observed a significantly better result in the control group (p < 0.01). Retrospectively, significantly more patients in the collagenase group would not choose the same therapy to treat DD (35 vs. 8%; p < 0.05). The two therapy options should be seen as complementary for the treatment of DD. Collagenase therapy seems a sensible option for DD with an earlier Tubiana stage and contractures that predominantly affect the MCP joint. Contractures with higher Tubiana stages that also affect the PIP joint should predominantly be treated with limited fasciectomy.

The Potential of Natural Products in Metabolic Disease Management: A Thorough Exploration of the Case of Uganda.

As Ugandans grapple with an increase in metabolic diseases, researchers are turning to their rich tradition of natural remedies. This review explores promising plants, such as Moringa oleifera, bridging the gap between the wisdom of Ugandan healers and modern science. Although these plants show potential, challenges remain. Many lack rigorous testing, standardized extracts, and long-term safety data. To unlock their true potential, a multipronged approach is needed. First, well-designed clinical trials are crucial to bringing together traditional healers and modern researchers. Imagine a Ugandan pharmacist precisely measuring a Moringa oleifera extract - this standardization ensures consistent results for future patients. Second, researchers need to delve deeper into how these plants influence the body. Finally, long-term safety studies are essential, especially when combined with medications. By following these steps, researchers can unleash the true power of Ugandan natural products. This empowers Ugandans to take control of their health. Future exploration of lesser-known plants and culturally sensitive education programs can further equip Ugandans on their way to well-being.

Post-COVID-19 neurogenic lower urinary tract dysfunction in pediatrics: A case series of clinical manifestations resembling Elsberg syndrome

Background: Among the numerous complications associated with post-coronavirus disease 2019 (COVID-19) syndrome, neurogenic lower urinary tract dysfunction (NLUTD) has been rarely reported, particularly in the pediatric population. Case Presentation: This report presented three pediatric cases of NLUTD that developed following severe acute respiratory syndrome coronavirus 2 infection, with clinical features resembling Elsberg syndrome. These cases were notable for the severity of their urinary symptoms, which required specialized and individualized bladder management strategies. This case series aims to raise awareness of this emerging clinical condition, which may be underdiagnosed in children recovering from COVID-19. Pediatricians, general practitioners, and urologists should be vigilant in considering NLUTD as a potential diagnosis in children presenting with new or unexplained urinary symptoms. Early diagnosis and timely management are crucial to address these symptoms effectively and prevent potential complications, such as irreversible upper urinary tract damage or long-term bladder dysfunction. Conclusion: Further investigations are warranted to clarify the pathophysiological mechanisms underlying post-COVID-19 NLUTD. Moreover, long-term follow-up studies are needed to better understand the natural history and implications of this condition in the pediatric population, as well as to develop effective strategies for prevention and treatment.

Designing for those who are Overlooked - Investigating long-term Impacts of Social Robotics for People with Advanced Dementia, Caregivers and Relatives in a Secured Care Environment

This paper presents the results of a long-term exploratory study that examined the social and emotional impact of using a social robot with a variety of applications to promote activities for people with advanced dementia and their caregivers. Qualitative data was collected over 10-months in a secured care environment in which we explored the appropriation of a social robot by 23 participants, including 13 PwAD, their family members, and professional caregivers. Our findings indicate that the use of the social robot demonstrated the potential to improve well-being, engagement, and social interaction of PwAD, even demonstrating the ability to create new memories and experiences. We discuss requirements and challenges in integrating social robots into secured care environments to provide a nuanced understanding for future research aiming for appropriate and inclusive care outcomes and socially embedded innovations that address the social actors involved and thus contribute to practical solutions for professional care.

Understanding the Lived Experiences of Women Underwent Female Genital Mutilation (FGM) in Bichena Town, North-Western Ethiopia

Female genital mutilation (FGM) remains a widespread and harmful traditional practice, despite legislative measures to protect girls and women’s rights in Ethiopia. The purpose of this qualitative study was to understand the experiences of women who underwent Female genital mutilation (FGM). Eight in-depth interviews were conducted with purposefully selected participants in Bichena town. Thematic analysis was used, and yielded three major themes: Reasons for continuity; Living with FGM; Location and Circumcisers. Findings of the study showed that participants identified limiting female sexuality, deep-rooted cultural beliefs and religion as justification for the continuity of FGM. The study also revealed that participants experienced mixed feelings; the majority reported experiences of loss of sexual desires, painful intercourse, and conflicts of spousal relationship, while some felt no negative consequences from the practice. Participants further reported that the operation of circumcision is performed using traditional circumcisers at parents’ home. The FGM experiences of these participants highlight the critical need for community-based intervention to cease this harmful customary practice. Long-term ethnographic study is required to validate our conclusion that FGM is one of the manifestations of gender-based human rights violation to control women’s sexuality and autonomy. Our study has special implications for adding knowledge in the social service research and providing insight on how to integrate FGM into an intervention area in social change practice

Enhancing work environments and reducing turnover intention: a multicenter longitudinal cohort study on differentiated nursing practices in Dutch hospitals

BackgroundAddressing the growing challenge of nurse retention requires coordinated actions at national and global levels to improve recruitment, retention policies, and investments in the nursing work environment. The nursing work environment, defined as the "organizational characteristics of a work setting that facilitate or constrain professional nursing practice", is critical in influencing whether nurses decide to leave their jobs. This study investigates the impact of differentiated nursing practices – which involved tailoring roles and responsibilities based on nurses’ training, skills, and experience in Dutch hospitals – and investigated their impact on the nursing work environment and turnover intention (i.e., nurses’ intentions to leave their organization). We also explored whether the work environment mediates this relationship.MethodsA multicenter longitudinal cohort study was conducted across 19 Dutch hospitals between 2019 and 2023. Nursing professionals participated via digital surveys administered before (T0) and after (T1) differentiated nursing practices were introduced. The nursing work environment was assessed using the Practice Environment Scale of the Nursing Work Index. A multilevel analysis with a random intercept and fixed slope was used to evaluate the impact of differentiated nursing practices on the work environment and on nurses' turnover intentions.ResultsWe received 5411 responses to our questionnaire – 4259 at T0 and 1152 at T1. Results showed that, while the overall work environment improved, particularly in the dimensions of staffing and resource adequacy, collegial nurse–physician relationships, and participation in hospital affairs, there were no significant improvements in nursing foundation for quality of care or nurse managers' ability, leadership, and support of nurses. Additionally, differentiated nursing practices did not significantly impact turnover intention, nor did the work environment mediate this relationship.ConclusionsThis study is the first to explore the unique effects of practice differentiation on turnover intention mediated by the work environment. The findings suggest that, while differentiated practices can enhance certain aspects of the work environment, a more systemic and integrated approach is required for sustained improvements. Future research should include longer term studies to fully understand the complex relationship and accompanying mechanisms between differentiated nursing practices, the nursing work environment, and turnover intention.Trial registrationClinical trial number not applicable.

Infectious keratitis following photorefractive keratectomy: a 13-year study at a tertiary center

IntroductionInfectious keratitis is a rare but devastating complication following photorefractive keratectomy (PRK) that may lead to visual impairment. This study assessed the clinical features, treatment strategies, and outcomes of post-PRK infectious keratitis.MethodsThis retrospective study was conducted on patients with post-PRK infectious keratitis presenting to Khalili Hospital, Shiraz, Iran, from June 2011 to March 2024. The study was conducted in two stages: the first stage assessed the incidence of post-PRK infectious keratitis among patients who underwent PRK at our center, while the second stage included all patients with post-PRK infectious keratitis, regardless of where their PRK was performed. The following data were collected: demographics, post-surgery presentation time, risk factors, culture results, treatments, follow-up duration, complications, and corrected distance visual acuity (CDVA) at admission and the last follow-up.ResultsForty-two patients (42 eyes) with a mean age of 28.74 years (male-to-female ratio of 1.2:1) were included. Among 38,938 PRK procedures performed at our center, the incidence of keratitis was estimated to be 0.018% (7/38,938). The odds of keratitis during the COVID-19 pandemic were 7.05 times higher (95% CI: 1.58 to 31.52, p-value = 0.015) than outside this timeframe (February 2020 to August 2023). Gram-positive bacteria were the most commonly isolated pathogens in microbiological studies, accounting for 45.2% (19/42) of cases. Early-onset infections were primarily caused by Staphylococcus aureus (9/26, 34.6%), Staphylococcus epidermidis (4/26, 15.4%), and Pseudomonas aeruginosa (4/26, 15.4%), whereas all of the cases with fungi (4/4, 100% (and Acanthamoeba (3/3, 100%) infections caused late-onset infections. All patients received broad-spectrum antibiotic therapy, followed by adjusted treatment based on microbial results. Cases developing endophthalmitis and those not responding to treatment or having non-resolving corneal scars required further interventions, such as penetrating keratoplasty and deep vitrectomy. The mean follow-up duration was 40.81 months, and 97.6% (41/42) of cases experienced CDVA improvement at follow-up.ConclusionThis long-term study found a post-PRK keratitis rate of 0.018%, with gram-positive bacteria as the most common pathogens. Prompt management and regular follow-up assessments are essential for achieving satisfactory outcomes.

Intermittent Fasting: Weighing the Benefits and Drawbacks for Health and Longevity – A Comprehensive Literature Review

Introduction:Obesity rates have risen sharply in recent decades, with one in eight people worldwide living with obesity in 2022. This has driven interest in the search for effective and sustainable dietary strategies. Intermittent fasting (IF), which alternates periods of eating and fasting, has gained considerable attention as a tool for weight management and overall health improvement. Unlike traditional calorie-restricted diets, IF’s simplicity and flexibility make it more accessible, potentially increasing adherence and long-term sustainability. Purpose:This study reviews current knowledge on IF, evaluating its physiological mechanisms, health benefits, risks, and long-term implications. It focuses on weight management, glucose metabolism, cardiovascular health, cognitive function, circadian rhythm, and sports performance, while addressing challenges related to safety and sustainability. Materials and Methods:This study reviews existing literature, including clinical trials, meta-analyses, and observational studies. It compares IF protocols such as alternate-day fasting (ADF), the 5:2 diet, and time-restricted eating (TRE), emphasizing physiological effects and practical implications. Conclusion:Intermittent fasting represents a promising dietary intervention with wide-ranging health benefits, from weight management to improved metabolic and cognitive health. However, challenges such as nutritional deficiencies, risks of hypoglycemia, and limited long-term data highlight the need for personalized approaches and further investigation. Future research should prioritize large-scale, long-term studies to refine fasting protocols and ensure safety and effectiveness across diverse populations. By addressing these gaps, IF could evolve into a sustainable, evidence-based strategy for improving global health outcomes.

Effects of Sewage Sludge Compost on Carbon, Nitrogen, Phosphorus, and Sulfur Ratios and Soil Enzyme Activities in a Long-Term Experiment

Effects of Sewage Sludge Compost on Carbon, Nitrogen, Phosphorus, and Sulfur Ratios and Soil Enzyme Activities in a Long-Term Experiment

Assessing the impacts of stakeholder involvement in long-term agricultural experiments via a case study in the upper US Midwest.

Agricultural researchers are increasingly encouraged to engage with stakeholders to improve the usefulness of their projects, but iterative research on the design and assessment of stakeholder engagement is scarce. The USDA Long-Term Agroecosystem Research (LTAR) Network recognizes the importance of effective engagement in increasing the utility of information and technologies for future agriculture. Diverse stakeholders and researchers at the Kellogg Biological Station (KBS) LTAR site co-designed the KBS LTAR Aspirational Cropping System Experiment, a process that provides a testing ground and interdisciplinary collaborations to develop theory-driven assessment protocols for continuous stakeholder engagement. Informed by prior work, we designed an assessment protocol that aims to measure participant preferences, experiences, and perceived benefits at various stages of this long-term project. Two online surveys were conducted in 2021 and 2022 among participants of LTAR engagement events at KBS, using a pre-post design, resulting in 125 total responses. Survey respondents had positive perceptions of the collaboratively designed research experiment. They had a strong expectation that the research would generate conservation and environmental advances while also informing policy and programs. Respondents also indicated a desire to network with other stakeholders. The research team noted the significant role of a long-term stakeholder engagement specialist in inviting participants from diverse backgrounds and creating an open and engaging experience. Overall, results highlight an interdisciplinary path of intentional and iterative engagement and evaluation to build a program that is adaptive and responsive to stakeholder needs.

Nurse-Assisted Remote Patient Monitoring for Self-Management Support to Patients With Long-Term Illness-A Qualitative Multimethod Study.

To explore patients and nurses' experiences of digital self-management support following participation in a remote patient monitoring intervention. An exploratory qualitative multimethod study. The study was conducted at two Norwegian university hospitals between January 2022 and February 2023. Data were obtained through semistructured interviews of 17 patients with heart failure, 10 patients surgically treated for colorectal cancer and eight nurses. The data collection also included excerpts from chat messages between patients and nurses obtained from the digital platform during January and February 2024. Data were analysed using abductive thematic analysis. The analysis revealed three themes: (1) raising illness awareness through RPM technology, (2) establishing a mutual collaboration in self-management challenges and (3) fostering a continued engagement in health behaviour change. The themes captured patients and nurses' experiences of receiving and providing digital self-management support. The analysis also identified a unifying key theme: 'bridging technology and self-management support through remote caring encounters', which firmly connected the three themes. Remote patient monitoring appeared to benefit both patients and nurses by altering patients' self-management routines and the nurses' workflows. Furthermore, applying the theory of technological competency as caring in nursing to remote patient monitoring interventions may help ensure that the patient-nurse relationship is not weakened as technology advances. Remote patient monitoring interventions have the potential to become a valuable tool in modern healthcare, enabling effective communication and collaboration between patients and nurses while also ensuring patient-centred care. However, future development of remote patient monitoring interventions should include nursing support. This study addresses remote patient monitoring and digital self-management support from the perspectives of both patients and nurses. The findings may have an impact on remote nursing, patient satisfaction and strategies to improve digital follow-up care for patients with long-term illnesses. The authors adhered to the EQUATOR guidelines through the SRQR reporting method. No patient or public contribution.

High-Temperature Corrosion Behavior of Superheater Materials at 600 °C: Insights from Laboratory and Field Exposures.

Combustion of biomass and waste results in release of corrosive species, such as alkali chlorides and water vapor, which accelerate the corrosion of superheaters in the boiler. To improve our understanding of alkali-induced corrosion, long-term corrosion investigations are needed. This study utilizes a systematic approach based on long-term corrosion studies (up to 8000 h) in a well-controlled laboratory environment to understand the corrosion behavior and protectiveness of oxide scales formed on a FeCr alloy (marginal chromia former) and three overlay weld coating systems (lean FeCrAl, FeCrAl, and Ni-based alloy) in a KCl-rich environment at 600 °C. The results show that all the alloys undergo fast breakaway corrosion and form duplex-layered scales consisting of outward- and inward-growing oxide scales. The marginal chromia former exhibits parabolic oxidation kinetics and forms Fe-rich oxides in the outer scale and mixed Fe- and Cr-oxides in the inner layer. These oxide microstructures are compared to the scales formed on probe-exposed samples in boilers, and similar microstructures are typically found after exposures. The FeCrAl coatings form Fe-rich oxides in the outer layer and Fe-, Cr-, and Al-oxides in the inner layer. All alloy systems except the Ni-based coating show corrosion rates in the boiler in good agreement with the laboratory test prediction. The Ni-based coating exhibits the slowest oxidation kinetics in the laboratory, forming thin oxide scales with Ni-rich oxides in the outer layer and Cr-rich oxides in the inner layers, while this is not the case in the waste-fired boiler.

Early Optimal Parenteral Nutrition During NICU Stay and Neurodevelopmental Outcomes in Very Preterm Infants: State of the Art.

Preterm infants (PIs) are more susceptible to neurodevelopmental impairment compared with term newborns. Adequate postnatal growth has been associated with improved neurocognitive outcomes; therefore, optimization of nutrition may positively impact the neurodevelopment of PIs. This study focused on macronutrient parenteral nutrition (PN) intake during the Neonatal Intensive Care Unit stay and their associations with neurodevelopmental outcomes in PIs in the first two years of life. The Embase, MEDLINE, and Cochrane Library databases were searched using the following subject headings and terms (MeSH): "premature infants", "parenteral nutrition", "growth", "brain", "neurodevelopment", and "central nervous system diseases". All relevant papers' reference lists were manually searched. PN and neurodevelopment studies concerning the first two years of life were collected and analyzed. 275 potential studies were retrieved, 64 were selected for full-text reading, and 22 were included (12 randomized controlled trials). While glucose intakes should be immediately provided and strictly monitored avoiding hyperglycemia, the long-term outcomes of aggressive PN caloric intakes are uncertain. Early amino acid (AA) supplementation is mandatory and improves short-term growth, though it is questionable whether increased AA and better neurodevelopment are directly related. Lipid infusion should be initiated right after birth, and further investigation will enable us to ascertain the potential impacts of lipid emulsions, particularly fish oil, on PI neurodevelopment. An aggressive PN and its possible metabolic complication could not favor neurodevelopment; the way forward could be a customized approach, depending on the patient's clinical state and tolerance. Long-term follow-up studies and the search for specific markers of tolerance are warranted.

Perennial Grasses on Stony Sandy Loam Arenosol: Summary of Results of Long-Term Experiment in Northern Europe Region (1995-2024).

Grasses can sustain soil functions despite nutrient depletion, which can have serious consequences for soil processes and ecosystem services. This paper summarizes the results of the long-term experiment (1995-2024) carried out in Arenosol within a temperate climate zone, focusing on the productivity of natural and managed grasslands; their succession changes over time, and so do the effects on soil chemical properties, and soil organic carbon (SOC) sequestration. The results indicated that two land uses-abandoned land (AL) and grassland fertilized with mineral fertilizers (MGf)-can be effectively applied to prevent Arenosol soil degradation. SOC accumulation occurs more rapidly in AL soils, and their chemical properties show less change over time. The ability of grasses to sequester SOC is better reflected by SOC stocks across the Ah horizon, where thickness varies over long-term grassland use. Significant changes in soil properties were observed more than 20 years after converting arable to herbaceous land use. While MGf has the highest biomass productivity, the use of fertilizers leads to soil acidification. The biomass productivity of AL and MGf increased with longer grassland use; however, in MG, productivity decreased without fertilizers, reaching AL's productivity levels after 20 years. As the age of AL increased, plant biodiversity decreased, and drought-resistant plants began to spread.

Efficacy of myo-inositol and zinc on insulin resistance in a paediatric population with obesity.

To assess the efficacy of the combined administration of myo-inositol and zinc, a mineral involved in the insulin pathway, in paediatric obesity with insulin resistance on HOMA-IR, glucose-insulin metabolism, and lipid profile. Double-blind, randomized, placebo-controlled study conducted in North Italy. Fifty-six patients (10-18 years, Tanner stage ≥3) with obesity and insulin resistance were randomized to myo-inositol (2000 mg), zinc gluconate (5 mg), and galactooligosaccharides (GOS) from plant-based origin (1000 mg) (TRT) or placebo (PLC) containing only GOS from plant-based origin (1000 mg). All patients received an isocaloric diet following the Mediterranean diet style. Data were collected at baseline (V0) and after 3 months (V1). The primary outcome was the insulin resistance index (HOMA-IR). Fifty out of 56 recruited subjects completed the study. TRT improved HDL cholesterol level compared to PLC (p = 0.05) but not insulin resistance. A stratified post hoc analysis was performed by sex, BMI, and subgroups of adherence to the Mediterranean diet. Subjects were divided for obesity grade, fasting insulin (p = 0.0137) and HOMA-IR (p = 0.0273) were lower in TRT than in PLC patients, with a greater effect on severe obesity. No adverse events were detected. Three months of supplementation with myo-inositol and zinc were beneficial on lipid profile and in managing obesity complications at least in subjects with severe phenotype. Thus, myo-inositol and zinc could be used as non-pharmacological agents. This work suggests a long-term study with a larger sample size to enrich the findings.

Ulcerative dermal necrosis of mature Atlantic salmon in the Kola and Tuloma rivers (Murmansk region): retrospective and prospects for studying the problem

The aim of the work is to analyze the results of virological and immunological studies, to track the dynamics of the entry and changes in external signs of pathology of Atlantic salmon into the Kola and Tuloma rivers.Methods used: fish size and weight indicators were determined using generally accepted methods. Methods for isolating viruses on continuous cell lines and their identification in RT-PCR reaction were used. Bactericidal activity of blood serum (BABS) was assessed using a nephelometric method. The content of non-specific immune complexes (IC) was established using a spectrophotometric method with selective precipitation with PEG-6000.According to the results of the work, it was revealed that in the Kola and Tuloma rivers, clinical manifestations of pathologies in fish have changed. Since 2021, typical symptoms of UDN have been replaced by signs of «red skin disease». The results of immunological studies indicate high activity of innate humoral immunity mechanisms in the examined individuals. The carriage of infectious pancreatic necrosis virus in Atlantic salmon populations living in the Kola and Tuloma rivers has been established. Clinical signs of pathology in Atlantic salmon are described in detail, and fish counting data for the Kola and Tuloma rivers are presented.The novelty of the work: for the first time, photographs of all noted typical signs of pathology, as well as the results of virological and immunological studies of Atlantic salmon in the Kola and Tuloma rivers for 2022–2024 are presented. A retrospective overview is given and prospects for studying the problem are outlined.Practical significance: the conducted research will allow creating a database on the results of comprehensive studies of Atlantic salmon diseases in the Kola and Tuloma rivers. Compare the results obtained in fish with and without clinical signs of pathologies over several years. Determine differences in the immune status of fish with different signs of pathology based on the results of long-term studies. This will help to understand how UDN disease develops over time and what causes it.