Introduction: Non-traumatic injuries are prevalent among longdistance cyclists. However, there is a lack of studies analysing the impact of different types of bikes on the pain experienced by cyclists or the physiotherapy measures commonly adopted by cyclists post-ride. Aim: To understand the impact of different types of bikes on the pain experienced by cyclists and to identify the physiotherapeutic measures commonly adopted by Indian cyclists. Materials and Methods: A cross-sectional survey was conducted on 120 cyclists (94 males and 26 females) from three different cycling groups in Gurugram, Haryana, India. Data was collected from August 2022 to September 2022. A selfstructured questionnaire was used to collect information on sociodemographic profile, type of bike used, duration of cycling, average distance and speed covered, areas and types of pain experienced, and measures taken to address pain. The data was analysed using Statistical Package for Social Sciences (SPSS) version 24.0. The level of significance was set at p≤0.05. Data was presented as frequencies and percentages, and comparisons were made using Kruskal-Wallis and Chi-square tests. Results: The mean age of the study participants was 39.36±11.88 years. The average speed and distance covered in one ride were 22.52±4.73 km/hour and 43.50±15.32 km, respectively. A statistically significant association between the type of bike used and pain status (p<0.05) was observed. 65% of riders reported experiencing pain in some part of their body in the last six months. Pain was more prevalent among hybrid bike users compared to mountain and road bike users. Male cyclists had a faster average speed compared to female cyclists by 5 km/hour. The average distance covered by male and female cyclists was similar, with just a 1 km difference. Cyclists commonly experienced pain in the wrists, legs, back, and neck. 19.1% of riders did nothing or only rested to address pain, while 21.66% opted for muscle stretching, 9.16% engaged in exercises, and 3.33% used cycling gloves for wrist and hand pain. Other measures included transcutaneous electrical nerve stimulation (TENS), dry needling, icing, refitting or changing the bike, and taking supplements. Conclusion: Among different types of bikes, hybrid bike users showed a higher incidence of pain. Cyclists in Gurugram tend to utilise limited physiotherapy measures to alleviate pain and enhance performance.