Logistics information system constitutes “computer-based information system that supports every aspect of the logistics management process, which involves the coordination of activities, such as scheduling, inventory replenishment, and material flow planning. It is regarded as an essential vehiclein achieving customer service in public entities. Such an information system enhances public institutions to share timely information and facilitate the delivery of products of the right quantity, quality, and at the desired location of the customers.This study examined the existing logistics information system in a bid to know the determinant factors for adoption of logistics information system with perceived benefits in the public health laboratory. The study involved two main theories which included diffusion of innovation and technological acceptance theories. The conceptual framework was developed to reflect the specific objectives mentioned. The study employed a quantitative cross-sectional research design whereby data were drawn from a simple random sampling of 90 respondents through structured questionnaires. Statistical Package for Social Science (SPSS) version 25 was adopted for quantitative analysis. Descriptive statistics, reliability analysis and regression analysis were used to analyze and determine the key factors and association in relation to dependent variables. The regression results show that there are significant and positive determinant factors of Logistics Information System adoption. The technological infrastructure (β=.371, t=5.296,p=.000), management support (β=-0.217, t=-2.092, p=.040), and government environmental support (β=0.990, t=11.911, p=.000) were all found significant and positive effects on Logistics Information System adoption as well as positive and significance between Logistics Information System adoption and perceived benefits. The study recommended that organization should focus on investing proper technological infrastructure