Locomotor Pattern Research Articles

Environmental enrichment reduces adgrl3.1-Related anxiety and attention deficits but not impulsivity

Environmental factors play a role in the development and severity of neuropsychiatric disorders. Externalizing disorders are characterized by disruptive, impulsive, and often aggressive behaviors, including difficulties with self-control, rule-breaking, and a tendency to act out in ways that may harm oneself or others. Externalizing disorders frequently co-occur with internalizing disorders, such as anxiety. Individuals experiencing both externalizing/internalizing disorders are often among the most likely to seek healthcare services, as this co-occurrence is associated with more severe symptomatology and greater functional impairment. Here, we investigated the impact of environmental enrichment (EE) on adgrl3.1, a gene associated with impulsivity and attention deficits in zebrafish (Danio rerio). This gene encodes a receptor involved in cell adhesion and signaling and has been linked to susceptibility to externalizing disorders. Zebrafish were reared in either standard or enriched environments (from 15 days-post fertilization), and attention, impulsivity, and anxiety-related phenotypes were assessed at adult stages (4 months-post fertilization) using the open field test and a 5-choice serial reaction time task. EE mitigated anxiety-related behaviors in adgrl3.1 knockouts, normalizing locomotor patterns and decreasing thigmotaxis. Although attention deficits were reduced in adgrl3.1-/- fish reared in EE, impulsive behaviors were not. Together, these findings suggest that while environmental enrichment (EE) mitigates externalizing and internalizing symptoms in adgrl3.1 mutants, impulsivity remains less responsive to EE used in this study, indicating its distinct resistance to modulation.

Dopamine neurons that inform Drosophila olfactory memory have distinct, acute functions driving attraction and aversion.

The brain must guide immediate responses to beneficial and harmful stimuli while simultaneously writing memories for future reference. While both immediate actions and reinforcement learning are instructed by dopamine, how dopaminergic systems maintain coherence between these 2 reward functions is unknown. Through optogenetic activation experiments, we showed that the dopamine neurons that inform olfactory memory in Drosophila have a distinct, parallel function driving attraction and aversion (valence). Sensory neurons required for olfactory memory were dispensable to dopaminergic valence. A broadly projecting set of dopaminergic cells had valence that was dependent on dopamine, glutamate, and octopamine. Similarly, a more restricted dopaminergic cluster with attractive valence was reliant on dopamine and glutamate; flies avoided opto-inhibition of this narrow subset, indicating the role of this cluster in controlling ongoing behavior. Dopamine valence was distinct from output-neuron opto-valence in locomotor pattern, strength, and polarity. Overall, our data suggest that dopamine's acute effect on valence provides a mechanism by which a dopaminergic system can coherently write memories to influence future responses while guiding immediate attraction and aversion.

Changes in Male Behavior in Response to Female Chemical Stimulus in an Understudied Arthropod Model

ABSTRACTIntraspecific sexual communication, crucial in reproductive interactions, often involves the interchange of signals like vibrations, visual signals, and chemical compounds like sex chemical cues. Within the arachnids, solifuges (“camel spiders”) are an understudied group in terms of the detection of chemical compounds during mate searching and sexual interactions. In solifugids, the malleoli, chemoreceptor structures located on the ventral side of legs IV, are putative structures capable of capturing chemical signals. Our study focused on chemical male perception of female cues in Titanopuga salinarum (Ammotrechidae), examining associated behavioral modifications and the role of malleoli. We analyzed alterations in the motion pattern (activity pulse) and specific behaviors performed by males in association with female chemical stimuli. Using experimental arenas, we exposed males to female chemical cues stimuli similar to those available at the field. Stimuli comprised females (F) and cuticular extracts (CE), with corresponding controls. Males with intact malleoli (N = 26 with F, N = 20 with CE) were compared to those with malleoli removed (N = 21 with F, N = 17 with CE). We found partial evidence that males detect chemical cues of females deposited on the substrate. We observed no differences in the duration and number of the activity pulses spent in the zones with and without stimuli in any of the groups analyzed. However, the males exhibit significant changes in locomotion patterns in response to female chemical cues, suggesting a behavioral response to these stimuli. This finding suggests that the males of this species detect the female chemical cues and modify their behavior, and can quickly gather the necessary olfactory information where the stimulus is located. In addition, we found a possible condition‐dependent regime for the detection of female cues by males, which would be expected from life‐history characteristics of T. salinarum. Our findings prompt discussion from a sexual selection perspective, suggesting the importance of chemical communication in intraspecific interactions in this elusive but fascinating animal model.

Early movement restriction impairs the development of sensorimotor integration, motor skills and memory in rats: Towards a preclinical model of developmental coordination disorder?

Children with neurodevelopmental disorders, such as developmental coordination disorder (DCD), exhibit gross to fine sensorimotor impairments, reduced physical activity and interactions with the environment and people. This disorder co-exists with cognitive deficits, executive dysfunctions and learning impairments. Previously, we demonstrated in rats that limited amounts and atypical patterns of movements and somatosensory feedback during early movement restriction manifested in adulthood as degraded postural and locomotor abilities, and musculoskeletal histopathology, including muscle atrophy, hyperexcitability within sensorimotor circuitry and maladaptive cortical plasticity, leading to functional disorganization of the primary somatosensory and motor cortices in the absence of cortical histopathology. In this study, we asked how this developmental sensorimotor restriction (SMR) started to impact the integration of multisensory information and the emergence of sensorimotor reflexes in rats. We also questioned the enduring impact of SMR on motor activities, pain and memory. SMR led to deficits in the emergence of swimming and sensorimotor reflexes, the development of pain and altered locomotor patterns and posture with toe-walking, adult motor performance and night spontaneous activity. In addition, SMR induced exploratory hyperactivity, short-term impairments in object-recognition tasks and long-term deficits in object-location tasks. SMR rats displayed minor alterations in histological features of the hippocampus, entorhinal, perirhinal and postrhinal cortices yet no obvious changes in the prefrontal cortex. Taken all together, these results show similarities with the symptoms observed in children with DCD, although further exploration seems required to postulate whether developmental SMR corresponds to a rat model of DCD.

Research on Motion Patterns of Soft Robots Based on Bionic Structure

Bionic soft robot is a new type of robot whose main body is composed of flexible material, with the advantages of adjustable size and strong environmental adaptability, which has a broad application prospect in logistics, medical care, resource exploration and other fields. Novel smart materials also shine in the design of soft robots. This paper highlights the research advancements in the locomotion patterns of bionic soft robots. The mechanism of movement of animals such as inchworms, starfish, earthworms, etc. and the soft robots designed to imitate them are introduced. Novel smart materials required to realise these designs, such as Shape Memory Alloy (SMA), dielectric elastomer (DE), a collapsible actuator (PFA), pNIPAM/CNTs hydrogel composite, are also presented. Methods to drive and control the motion of these soft robots are presented, including thermally driven shape memory alloys, pneumatic airbags, and laser-driven, magnetic field-driven, and electrically driven dielectric materials, among other types. After discussing the materials and methods, the current challenges to the innovation of motion patterns for bionic soft robots are analyzed.

Rethinking the four-wing problem in plesiosaur swimming using bio-inspired decentralized control

A locomotor system that can function across different environmental conditions and produce a range of performances is one of the most critical abilities needed for extant and extinct animals in order to survive and maximise their competitive fitness. Recent engineering-inspired paleontological studies have reconstructed feasible locomotor patterns in extinct animals. However, it is still challenging to describe how extinct animals successfully adjust their locomotor patterns to new situations (e.g., changes in locomotor speed and morphology). In this study, we develop a novel reconstruction method based on a bio-inspired control system. We focus on plesiosaurs, an extinct aquatic reptile group which has two pairs of flipper-shaped limbs, and demonstrate that a highly optimised, flexible locomotor pattern for all four flippers can be reconstructed based on a decentralized control scheme formulated from extant animals’ locomotion. The results of our robotic experiments show that a simple, local sensory feedback mechanism allows the plesiosaur-like robot to exploit the fluid flow between the flippers and generate efficient swimming patterns in response to changes in locomotor conditions. Our new method provides further evidence how decentralized control systems can produce a pathway between extinct and extant animals in order to understand the how extinct animals moved and how these movement patterns may have evolved.

Are we assessing motor competence? Evidence-informed constructs for motor competence in preschoolers through an Exploratory Graph Analysis

ABSTRACT Motor competence (MC) is conceptually defined as a multidimensional latent construct that covers the proficient performance in motor skills and its underlying mechanisms This study aimed to statistically provide arguments that MC is a network of interconnected constructs, such as FMS, coordination, and its underlying mechanisms, which are responsible for preschoolers’ proficiency in motor tasks. Participated 102 preschoolers (65 girls, M age = 4.22 ± 0.19) who were assessed for the Test of Gross Motor Development − 2nd edition, the Motor Competence Assessment, and the Supine-to-Stand. Data were explored using Exploratory Graph Analysis, using the EGAnet package in RStudio. A four-dimensional structure (61.2% of interactions) comprising tasks of the different protocols was underlined, in which all the nodes presented stable and adequate indexes (≥0.65; TEFI = –2.67). Four dimensions of MC were highlighted, namely Dimension 1, which combined movements for locomotor patterns; Dimension 2, comprising three process-oriented measures of object control skills to project objects; Dimension 3, which comprised of skills which require body coordination to displace body through space; and Dimension 4, composed by object control skills evaluated through product-oriented measures. For a better understanding of MC, the assessment of these different aspects that comprises MC should be considered.

Design and analysis of a thick-panel origami-inspired soft crawling robot with multiple locomotion patterns

Abstract Due to the flexibility obtained through both materials and structures, soft robots have wide potential applications in complicated internal and external environments. This paper presents a new soft crawling robot with multiple locomotion patterns that integrate inchworm motion and various turning motions. First, the conceptual design of the proposed robot is presented by introducing thick-panel origami into the synthesis of a crawling robot, resulting in a Waterbomb-structure-inspired hybrid mechanism. Second, all locomotion patterns of the robot are precisely described and analyzed by screw theory in an algebraic manner, which include inchworm motion, restricted planar motion, quantitative turning motion, and marginal exploration motion. Then, the output motion parameter for each locomotion pattern is analytically modeled as a function of the robotic dimensional parameters, and the robot can thus be designed and controlled in a customized way for the expected output motion. Finally, the theoretical analysis and derivations are validated by simulation and physical prototype building, which lay the foundations for the design and manufacture of small-scale soft crawling robots with precise output motions in a complex planar environment.

Implications of Lead (Pb)-Induced Transcriptomic and Phenotypic Alterations in the Aged Zebrafish (Danio rerio).

Lead (Pb) is a well-known neurotoxin with established adverse effects on the neurological functions of children and younger adults, including motor, learning, and memory abilities. However, its potential impact on older adults has received less attention. Using the zebrafish model, our study aims to characterize the dose-response relationship between environmentally relevant Pb exposure levels and their effects on changes in behavior and transcriptomics during the geriatric periods. We exposed two-year-old zebrafish to waterborne lead acetate (1, 10, 100, 1000, or 10,000 µg/L) or a vehicle (DMSO) for 5 days. While lower concentrations (1-100 µg/L) reflect environmentally relevant Pb levels, higher concentrations (1000-10,000 µg/L) were included to assess acute toxicity under extreme exposure scenarios. We conducted adult behavior assessment to evaluate the locomotor activity following exposure. The same individual fish were subsequently sacrificed for brain dissection after a day of recovery in the aquatic system. RNA extraction and sequencing were then performed to evaluate the Pb-induced transcriptomic changes. Higher (1000-10,000 ug/L) Pb levels induced hyperactive locomotor patterns in aged zebrafish, while lower (10-100 ug/L) Pb levels resulted in the lowest locomotor activity compared to the control group. Exposure to 100 µg/L led to the highest number of differentially expressed genes (DEGs), while 10,000 µg/L induced larger fold changes in both directions. The neurological pathways impacted by Pb exposure include functions related to neurotransmission, such as cytoskeletal regulation and synaptogenesis, and oxidative stress response, such as mitochondrial dysfunction and downregulation of heat shock protein genes. These findings emphasize a U-shape dose-response relationship with Pb concentrations in locomotor activity and transcriptomic changes in the aging brain.

Influence of Physical Characteristics of Obstacles on the Locomotor Pattern of Older Adults at Higher Risk of Falling

ObjectiveThis study aimed to investigate the locomotor behavior of older adults with and without a history of falls as they avoided obstacles with different physical characteristics. MethodsTwenty-one older adults participated in this study. The group was divided into higher risk of falling (n = 10) and lower risk of falling (n = 11). The following conditions were carried out: (1) walking and avoiding a solid obstacle and (2) walking and avoiding a fragile obstacle. ResultsOlder adults at higher risk of falling had worse performance during the obstacle approach phase. Both groups performed worse when avoiding the fragile obstacle than when avoiding the solid obstacle. Older adults at higher risk of falling had their feet closer when avoiding the obstacle, and both groups raised their limbs higher to avoid a fragile obstacle. Older adults at higher risk of falling were closer to obstacles after avoiding them. ConclusionFor the participants in this study, the physical characteristics of the obstacle did not interfere with the locomotor performance of older adults during obstacle avoidance. This study found that older adults at higher risk of falls modulated their locomotor pattern before avoiding the obstacles, presenting lower velocity, shorter step length, and greater step width compared with older adults at lower risk of falling.

Magnetically Driven Quadruped Soft Robot with Multimodal Motion for Targeted Drug Delivery.

Untethered magnetic soft robots show great potential for biomedical and small-scale micromanipulation applications due to their high flexibility and ability to cause minimal damage. However, most current research on these robots focuses on marine and reptilian biomimicry, which limits their ability to move in unstructured environments. In this work, we design a quadruped soft robot with a magnetic top cover and a specific magnetization angle, drawing inspiration from the common locomotion patterns of quadrupeds in nature and integrating our unique actuation principle. It can crawl and tumble and, by adjusting the magnetic field parameters, it adapts its locomotion to environmental conditions, enabling it to cross obstacles and perform remote transportation and release of cargo.

Developing Engaging Audio-Visual Learning Media for Basic Locomotor Patterns through Play-Based Activities for Early Learners

Developing Engaging Audio-Visual Learning Media for Basic Locomotor Patterns through Play-Based Activities for Early Learners

Cortical networks of parkinsonian gait: a metabolic and functional connectivity study.

Locomotion is an automated voluntary movement sustained by coordinated neural synchronization across a distributed brain network. The cerebral cortex is central for adapting the locomotion pattern to the environment and alterations of cortical network dynamics can lead to gait impairments. Gait problems are a common symptom with a still unclear pathophysiology and represent an unmet therapeutical need in Parkinson's disease. Little is known about the cortical network dynamics of locomotor control in these patients. We studied the cortical basis of parkinsonian gait by combining metabolic brain imaging with high-density EEG recordings and kinematic measurements performed at rest and during unperturbed overground walking. We found significant changes in functional connectivity between frontal, sensorimotor, and visuomotor cortical areas during walking as compared to resting. Specifically, hypokinetic gait was associated with poor information flow from the supplementary motor area (SMA) to precuneus and from calcarine to lingual gyrus, as well as high information flow from calcarine to cuneus. Our findings support a role for visuomotor integration processes in PD-related hypokinetic gait and suggest that reinforcing visual information may act as a compensatory strategy to allow SMA-mediated feedforward locomotor control in PD.

Mycotoxin zearalenone induces multi-/trans-generational toxic effects and germline toxicity transmission via histone methyltransferase MES-4 in Caenorhabditis elegans

Zearalenone (ZEN), an endocrine-disrupting mycotoxin, is prevalent and persists in the environment. ZEN has the potential to cause adverse health impacts extending over generations, yet there is a lack of relevant research. Therefore, we explored the ZEN-induced multi-/trans-generational locomotive and reproductive toxicities, as well as the underlying epigenetic mechanisms in Caenorhabditis elegans. In multi-generational analysis, the evolution tendency and toxicity latency were observed under sustained exposure to 0.1 and 1 μM ZEN across five generations (P0-F4). The toxic effects were found in filial generations even if the initial parental exposure showed no apparent effects. Trans-generational results indicated the toxic inheritance phenomenon of 10 and 50 μM ZEN, where a single generation of ZEN exposure was sufficient to affect subsequent generations (F1-F3). Additionally, the pattern of locomotion was relatively sensitive in both generational studies, indicating varying sensitivity between indicators. Regarding epigenetic mechanism of toxicity transmission, ZEN significantly decreased the parental expression of histone methyltransferase encoded genes set-2, mes-2, and mes-4. Notably, the downregulation of mes-4 persisted in the unexposed F1 and F2 generations under trans-generational exposure. Furthermore, the mes-4 binding and reproduction-related rme-2 also decreased across generations. Moreover, parental germline specific knockdown of mes-4 eliminated the inherited locomotive and reproductive toxic effects in offspring, showing that mes-4 acted as transmitter in ZEN-induced generational toxicities. These findings suggest that ZEN is an epigenetic environmental pollutant, with a possible genetic biomarker mes-4 mediating the germline dependent transmission of ZEN-triggered toxicity over generations. This study provides significant insights into ZEN-induced epigenotoxicity.

Frontoparietal-Striatal Network and Nucleus Basalis Modulation in Patients With Parkinson Disease and Gait Disturbance.

Neural computations underlying gait disorders in Parkinson disease (PD) are multifactorial and involve impaired expression of stereotactic locomotor patterns and compensatory recruitment of cognitive functions. This study aimed to clarify the network mechanisms of cognitive contribution to gait control and its breakdown in patients with PD. Patients with PD were instructed to walk at a comfortable pace on a mat with pressure sensors. The characterization of cognitive-motor interplay was enhanced by using a gait with a secondary cognitive task (dual-task condition) and a gait without additional tasks (single-task condition). Participants were scanned using 3-T MRI and 123I-ioflupane SPECT. According to gait characteristics, cluster analysis assisted by a nonlinear dimensionality reduction technique, t-distributed stochastic neighbor embedding, categorized 56 patients with PD into 3 subpopulations. The preserved gait (PG) subgroup (n = 23) showed preserved speed and variability during gait, both with and without additional cognitive load. Compared with the PG subgroup, the mildly impaired gait (MIG) subgroup (n = 16) demonstrated deteriorated gait variability with additional cognitive load and impaired speed and gait variability without additional cognitive load. The severely impaired gait (SIG) subgroup (n = 17) revealed the slowest speed and highest gait variability. In addition, group differences were found in attention/working memory and executive function domains, with the lowest performance in the SIG subgroup than in the PG and MIG subgroups. Using resting-state functional MRI, the SIG subgroup demonstrated lower functional connectivity of the left and right frontoparietal network (FPN) with the caudate than the PG subgroup did (left FPN, d = 1.21, p < 0.001; right FPN, d = 1.05, p = 0.004). Cortical thickness in the FPN and 123I-ioflupane uptake in the striatum did not differ among the 3 subgroups. By contrast, the severity of Ch4 density loss was significantly correlated with the level of functional connectivity degradation of the FPN and caudate (left FPN-caudate, r = 0.27, p = 0.04). These findings suggest that the functional connectivity of the FPN with the caudate, as mediated by the cholinergic Ch4 projection system, underlies the compensatory recruitment of attention and executive function for damaged automaticity in gait in patients with PD.

Genomic and single-cell analyses reveal genetic signatures of swimming pattern and diapause strategy in jellyfish

Jellyfish exhibit innovative swimming patterns that contribute to exploring the origins of animal locomotion. However, the genetic and cellular basis of these patterns remains unclear. Herein, we generated chromosome-level genome assemblies of two jellyfish species, Turritopsis rubra and Aurelia coerulea, which exhibit straight and free-swimming patterns, respectively. We observe positive selection of numerous genes involved in statolith formation, hair cell ciliogenesis, ciliary motility, and motor neuron function. The lineage-specific absence of otolith morphogenesis- and ciliary movement-related genes in T. rubra may be associated with homeostatic structural statocyst loss and straight swimming pattern. Notably, single-cell transcriptomic analyses covering key developmental stages reveal the enrichment of diapause-related genes in the cyst during reverse development, suggesting that the sustained diapause state favours the development of new polyps under favourable conditions. This study highlights the complex relationship between genetics, locomotion patterns and survival strategies in jellyfish, thereby providing valuable insights into the evolutionary lineages of movement and adaptation in the animal kingdom.

Studies on embryo development and larval feeding of clam Paphia textile

The present study investigated the embryonic development, growth, metamorphosis, locomotory patterns, and feeding behaviors of Paphia textile larvae, offering dietary suggestions based on observed patterns. The larvae were subjected to a dietary regimen consisting of nine microalgae species at various developmental stages (1, 4, 7, 10, 13, and 16 days post-hatching). One hour after each feeding session, their feeding patterns were carefully documented. To assess digestion, larvae that had consumed the microalgae were filtered, rinsed, and placed in filtered seawater. The embryonic development of P. textile encompassed distinct stages, including the fertilized egg, emergence of the first and second polar bodies, progression through the 2–32 cell stage, morula stage, blastula stage, gastrula stage, trochophore stage, and ultimately the D-larvae stage. Movement patterns revealed that the larvae primarily utilized their cilia for locomotion and feeding. From a transverse perspective, they moved clockwise, tracing an irregular circular path. Vertically, they exhibited spiral movements, ascending or descending as needed. As they entered the late stage, they transitioned to using reciprocal expansion of their axe feet for crawling and filtering food through their gills. Throughout their development, the larvae were fed a variety of microalgae species, including Chlorella vulgaris, Nannochloropsis oculata, Pavlova viridis, Dicrateria zhanjiangensis, and Isochrysis galbana. At 4 days old, they began consuming Dunaliella salina, and at 7 days, they incorporated Platymonas subcordiformis into their diet. By 10 days of age, they had expanded their food choices to include Chaetoceros muelleri. However, it was noted that larvae frequently suffered from indigestion or incomplete digestion when fed excessive amounts of C. vulgaris and N. oculata. Therefore, it is recommended to commence the larval culture of P. textile using P. viridis, D. zhanjiangensis, and I. galbana as the primary dietary sources. Furthermore, a feeding strategy emphasizing small, frequent meals is recommended to promote optimal digestion and larval health.

Sleep benefits perceptual but not movement-based learning of locomotor sequences

Practicing complex locomotor skills, such as those involving a step sequence engages distinct perceptual and motor mechanisms that support the recall of learning under new conditions (i.e., skill transfer). While sleep has been shown to enhance learning of sequences of fine movements (i.e., sleep-dependent consolidation), here we examined whether this benefit extends to learning of a locomotor pattern. Specifically, we tested the perceptual and motor learning of a locomotor sequence following sleep compared to wake. We hypothesized that post-practice sleep would increase locomotor sequence learning in the perceptual, but not in the motor domain. In this study, healthy young adult participants (n = 48; 18–33 years) practiced a step length sequence on a treadmill cued by visual stimuli displayed on a screen during training. Participants were then tested in a perceptual condition (backward walking with the same visual stimuli), or a motor condition (forward walking but with an inverted screen). Skill was assessed immediately, and again after a 12-h delay following overnight sleep or daytime wake (n = 12 for each interval/condition). Off-line learning improved following sleep compared to wake, but only for the perceptual condition. Our results suggest that perceptual and motor sequence learning are processed separately after locomotor training, and further points to a benefit of sleep that is rooted in the perceptual as opposed to the motor aspects of motor learning.

3D Printed Swordfish‐Like Wireless Millirobot

Inspired by the efficient swimming capabilities of swordfish, a novel wireless soft swordfish‐like robot with programmable magnetization has been developed, integrating direct‐ink‐writing (DIW) 3D printing and assembly technology. This 20 mm long robot features a streamlined form and magnetically programmable movements, enabling biomimetic locomotion patterns such as straight‐line swimming and turning swimming. The robot includes a silicone‐based torso (body, abdomen, and pectoral fin) and a crescent‐shaped tail fin made from a magnetically programmable polymer embedded with neodymium‐iron‐boron (NdFeB) particles. The tail fin, fabricated by multi‐material alternating printing to achieve a gradient magnetism distribution, is controlled by an external magnetic field to mimic the rapid oscillation of a swordfish's tail, achieving a swimming speed of 0.51 BL/ s. The tail fin's asymmetric oscillation amplitudes, adjusted by magnetic field control, allow the robot to transition seamlessly from high‐speed straight swimming to agile turning. The robot can perform tracking swimming along specific planned paths, such as “C” and “Z” shaped trajectories. Potential applications include environmental monitoring and targeted drug release. The multi‐material 3D printing technology enhances the robot's efficiency and sensitivity in simulating natural biological movements, extending to the design and development of various flexible devices and soft robots.

Footfall patterns and stride parameters of Common hippopotamus (Hippopotamus amphibius) on land.

Common hippopotamuses (hippos) are among the largest extant land mammals. They thus offer potential further insight into how giant body size on land influences locomotor patterns and abilities. Furthermore, as they have semi-aquatic habits and unusual morphology, they prompt important questions about how locomotion evolved in Hippopotamidae. However, basic information about how hippos move is limited and sometimes contradictory. We aimed to test if hippos trot at all speeds and if they ever use an aerial (suspended) phase, and to quantify how their locomotor patterns (footfalls and stride parameters) change with approximate speed. We surveyed videos available online and collected new video data from two zoo hippos in order to calculate the data needed to achieve our aims; gathering a sample of 169 strides from 32 hippos. No hippos studied used other than trotting (or near-trotting) footfall patterns, but at the fastest relative speeds hippos used brief aerial phases, apparently a new discovery. Hippos exhibit relatively greater athletic capacity than elephants in several ways, but perhaps not greater than rhinoceroses. Our data help form a baseline for assessing if other hippos use normal locomotion; relevant to clinical veterinary assessments of lameness; and for reconstructing the evolutionary biomechanics of hippo lineages.