Most of studies dealing with hantaviruses have focused on people affected by this disease. Few studies have dedicated in the role of the land use and land cover (LULC) type in the hantavirus occurrences. The objective of this study is to analyze the influence of environmental factors in the incidence of hantavirus in the Federal District of Brazil. Probable sites of infection were identified based on the global Moran index (Moran I), local Moran index, and Getis-Ord Gi* spatial statistics analysis. Results showed that the Paranoá, Planaltina, and São Sebastião administrative regions are the critical regions in terms of occurrence of hantaviruses in the study area. altitude, categories of transition, LULC type, and geomorphology were the most important parameters related to the occurrence of the hantavirus disease. This study provides information on the effects of landscape and climatic factors on the incidence of hantavirus. The information can be used to better understand how hantavirus cardiopulmonary syndrome (HCPS) behaves in the Federal District, providing assistance for public health monitoring strategies and epidemiological surveillance.