In the present article, we analyzed the unsteady bio-convective hybridized micropolar nanofluid flow over vertical exponentially stretching surface under the stratification conditions. Hybrid nanoparticles (AA7075 + AA7072 alloys) with a base fluid of water are deliberated in the current study. The Cattaneo–Christov heat flux and activation energy is taken into account to examine the heat and mass transfer rate. The appropriate transformations are used to transfer the flow model into the coupled non—linear ODEs. The Bvp4c Matlab technique is used to solve the coupled equations. The conclusion of various parameters is examined graphically. The physical quantities like skin friction, local Sherwood number and local microorganisms number observed via graphs. It is worth noticing that the axial and angular velocity reduces near the boundary and enhances away from the boundary with the escalation of solid volume fraction single and multi wall carbon nanotubes. Further, the skin factor, Sherwood number, and microorganism number show increasing behavior as increasing stretching parameter and solid volume fraction.