Local Immune Factors Research Articles

Phototherapy is a pathogenetic method in the treatment of acute sinusitis

Currently, physical treatment methods, in particular use of photochromotherapy in the treatment of sinusitis, are of interest. Is to investigate the local effect of light in the visible range (450 nm) on the factors of local infection protection of the nasal mucosa. The study involved 50 patients with a diagnosis of acute rhinosinusitis at the age of 27.23±3.29 years. Exposure of the mucous membrane of the nasal cavity was carried out in accordance with the parameters: 450 nm wavelength (monochromatic non-polarized blue light), during 5 minutes for each nostril. Sessions of photochromotherapy were carried out according to the "Sanitary norms and rules for the design and operation of lasers" No. 5804-91. Inclusion of photochromotherapy in the complex of therapeutic measures for patients with acute rhinosinusitis helps to reduce the total number of neutrophils in nasal secretion, normalize their lysosomal activity, NBT-reducing activity, activity and intensity of phagocytosis. Restoration of local immunity factors in nasal mucosa, expressed in normalizing the functional and metabolic status of neutrophil granulocytes of the nasal secretion, can serve as one of the criteria for the effectiveness of treatment using photochromotherapy

Effect of Esberitox phytopreparation on factors of local immunity of the oral cavity

Effect of Esberitox phytopreparation on factors of local immunity of the oral cavity

LOCAL IMMUNITY STATUS OF OROPHARYNX IN PATIENTS WITH SCLEROMA

Scleroma is a rare chronic granulomatous disease of the upper respiratory tract caused by Klebsiella pneumoniae subsp. rhinoscleromatis. To date its pathogenesis is as yet little understood. At the same time, scleroma is associated with a number of immune system disturbances. The aim: To study local immunity status of oropharynx in patients with scleroma, and to compare its parameters in various clinical forms of the disease. 20 apparently healthy subjects and 92 patients with scleroma (33 males, 59 females) underwent clinical immunologic evaluation. There were 31 patients with dominating infiltrative form of scleroma, 30 – with dominating atrophic form, 31 – with dominating scarring form. Concentration of secretory and monomeric immunoglobulin A, immunoglobulin G, α-interferon, interleukin 1β in oropharyngeal secretion was determined by enzyme immunoassay. Patients with scleroma were found to have altered local immunity of oropharyngeal secretion. There was a strong tendency for decreased concentration of secretory immunoglobulin A - 1.3-2.0 times, and decreased immunoglobulin G level – 1.5-2.3 times (р < 0.05) as compared to the values in healthy subjects. Specific features of local immunity in oropharyngeal secretion in various forms of scleromatous inflammatory process in upper respiratory tract were found: the most significant decrease of α-interferon concentration in atrophic and scarring forms of the disease, and the largest increase of anti-inflammatory interleukin 1β and immune complex concentration in infiltrative form of scleroma. The study revealed deficiency of local immunity factors in oropharynx, being indicative of immunopathogenetic role of diagnosed disturbances in development and persistence of chronic inflammation in scleroma, and emphasizing the necessity of immunocorrection in complex therapy of the disease.

Association between interleukin-2, interleukin-10, secretory immunoglobulin A and immunoglobulin G expression in vaginal fluid and human papilloma virus outcome in patients with cervical lesions.

The present study was designed to investigate the association between a change in vaginal local immunity and human papilloma virus (HPV) infection outcome in patients with cervical lesions, through the study of the expression of vaginal local immune factors, interleukin (IL)-2, IL-10, secretory immunoglobulin A (sIgA) and IgG, in patients with different grades of cervical lesions and different degrees of cervical lesions caused by HPV infection prior to and following treatment. The experimental group comprised 136 patients with low-grade squamous intraepithelial lesions, 236 patients with high-grade squamous intraepithelial lesions and 133 patients with cervical squamous cell carcinoma, while the control group comprised 100 time- and location-matched healthy women. The concentrations of sIgA, IgG, IL-2 and IL-10 in the vaginal lavage fluid, were detected using ELISA prior to treatment and at 3, 6 and 12 months after treatment. Prior to treatment, differences in HPV infection rate and changes in vaginal immune factors between patients with all grades of lesions and controls were statistically significant (P<0.05). Furthermore, IL-2 and IL-10 expression levels and the IL-2/IL-10 ratio in patients with different grades of lesions, with or without seroconversion, were significantly different to those in controls (P<0.05). However, the differences between changes in IgG and sIgA expression between patients with HPV seroconversion and patients with persistent HPV infection were not statistically significant (P>0.05). The results of the present study suggest that the restoration of humoral immune function promotes HPV seroconversion, and that IL-2 and IL-10 levels and their ratio may reflect the severity of cervical lesions and treatment effects to a certain extent.

Network pharmacology-based identification of the key mechanism of Qinghuo Rougan Formula acting on uveitis

BackgroundQinghuo Rougan Formula (QHRGF) is a traditional Chinese medicine (TCM) that has been widely apllied to treat uveitis for several decades. However, the inhibitory mechanism of QHRGF in uveitis has remained to be an enigma. MethodsThe Chinese herbal medicine pharmacology data and analysis platform wereused to search and screen for the effective components of the QHRGF compound injection and to analyse possible therapeutic targets based on network topology. In addition, various known disease target databases were enraolled, the therapeutic target proteins in uveitis were screened, and a protein-protein interaction (PPI) network was constructed. Enrichment analysis was performed on key nodes. Finally, the inhibitory effect of QHRGF on uveitis was verified by experiments. ResultsWe identified 259 major candidate targets of QHRGF and successfully constructed a ‘QHRGF-compound-target-uveitis’ network. Above-mentioned targets revealed by Gene enrichment analysis have played an significant role in the cell cycle, autoimmune disease, apoptosis and related signal pathways. We demonstrated that QHRGF attenuates local inflammation in experimental autoimmune uveoretinitis (EAU) rats by regulating natural killer T (NKT) cells and inhibiting MAPK signal pathways. ConclusionQHRGF may regulate the local immune response and inflammatory factors mainly through the MAPK signal pathway. For autoimmune uveitis, QHRGF may be a promising, long-lasting treatment strategy.

СОСТОЯНИЕ КЛЕТОЧНОГО И ГУМОРАЛЬНОГО ЗВЕНЬЕВ ИММУНИТЕТА ПРИ КАНДИДА-АССОЦИИРОВАННОМ ПАРОДОНТИТЕ СО СРЕДНЕЙ И ВЫСОКОЙ СТЕПЕНЬЮ КОЛИЧЕСТВЕННОЙ ОБСЕМЕНЕННОСТИ ПАРОДОНТАЛЬНЫХ КАРМАНОВ

According to many researchers, the change in the course of chronic generalized periodontitis is associated with changes in the patient's immune status. As a result, colonization resistance decreases, the etiological role of opportunistic pathogens, fungal associations, which act synergistically with periodontal pathogenic bacteria and contribute to the development of candida-associated periodontitis, increases.&#x0D; Due to the fact that this disease has little specific symptoms, relapsing course and does not respond to standard therapy, characteristic disorders in the immune system can serve as a diagnostic criterion as well as a criterion for evaluating the effectiveness of the treatment. However, to date, this issue has not been studied, taking into account the degree of quantitative contamination of periodontal pockets with fungi of the genus Candida, which was the subject of this study. &#x0D; The aim is to study the state of cellular and humoral immunity during the exacerbation of candida - associated periodontitis with an average and high degree of dissemination of periodontal pockets by Candida fungi. &#x0D; Methodology. A survey of cellular and humoral immunity units of 25 people with intact periodontal disease and 60 patients with exacerbation of candida-associated periodontitis, taking into account the degree of dissemination of periodontal pockets by Candida fungi. Modern and informative immunological methods of research have been used, such as T and B lymphocytes and their subpopulations, phagocytic unit, humoral factors of systemic and local immunity. &#x0D; Results. An immunological study revealed that the exacerbation of candida-associated periodontitis is accompanied by changes in cellular and humoral immunity. The severity and nature of the changes depend on the degree of dissemination of periodontal pockets by Candida fungi.&#x0D; Conclusions. The obtained data on changes in the indices of cellular and humoral immunity can serve as a diagnostic criterion and a criterion for evaluating the effectiveness of the treatment being carried out.

ОЦЕНКА РЕЗУЛЬТАТОВ КОМПЛЕКСНОЙ ТЕРАПИИ КРАСНОГО ПЛОСКОГО ЛИШАЯ СЛИЗИСТОЙ ОБОЛОЧКИ РТА С ИСПОЛЬЗОВАНИЕМ АУТОЛОГИЧНОЙ ПЛАЗМЫ И ГИДРОЛИЗАТА ПЛАЦЕНТЫ ЧЕЛОВЕКА

Subject. On the basis of a comprehensive dental examination, the clinical condition of the oral mucosa was assessed. With the use of laboratory research methods, the parameters of the microta state from the surface of erosion, the state of local immunity of the oral fluid, as well as anamnesis data with the assessment of somatic status, clinical blood analysis, ultrasound examination of the state of internal organs, the tactics of the complex of phased local and General therapy were studied. In clinical conditions, the analysis of minimizing pain, the nature of the epithelialization of erosive elements and regeneration of the oral mucosa. Using the analysis of laboratory methods, the pronounced antibacterial effect, increase of local immunity factors of the oral cavity, a qualitative decrease in chronic inflammation in the oral mucosa and in the body as a whole, as well as a decrease in the depressive state of the patient were evaluated. Proven and proved the efficiency of application of this complex treatment in the early and late periods.&#x0D; The purpose ― is to draw the attention of practicing dentists and related specialists to the need for an effective phased scheme of diagnostic and therapeutic and preventive measures in patients with lichen planus.&#x0D; Material and methods. The article is based on the description of clinical observation of a patient with erosive-ulcerative form of lichen planus.&#x0D; Results. The use of the proposed complex of step-by-step local injection methods on the oral mucosa and General measures by dermatologists, therapists, gastroenterologists in the erosive and ulcerative form of lichen planus contributes to a better acceleration of the epithelialization process, regeneration, reducing the signs of chronic inflammation, qualitative improvement of the somatic status and quality of life of patients.&#x0D; Summary. The inclusion in the scheme of the complex of local and General therapeutic and preventive measures of erosive-ulcerative form of lichen planus by injection of autologous plasma (AP) and human placenta hydrolysate (HRH) is a more effective method of therapy in these patients.

Factors of local immunity in patients with rectal cancer after prolonged radiotherapy.

e15164 Background: Immunological study of the blood and tumor tissues was performed in patients with rectal cancer receiving neoadjuvant chemoradiotherapy. Methods: 30 patients with rectal cancer (13 women and 17 men aged 37-68 years with stage II-III adenocarcinomas G1-G3) were divided into groups according to results of DNA cytometry and their response to neoadjuvant treatment. When tumor proliferative activity was stable for 4 weeks of treatment, patients received surgery on time (group 1), while patients with verified inhibition of tumor proliferation (the index decrease by 1.5 times and more) continued treatment for 6-8 weeks and then were operated on (group 2). The immune status of patients (T, B, NK, DN, Tregs) was assessed during treatment. Homogenates of tumor tissue samples obtained during surgery were studied for the levels of lymphocytes (flow cytometry) and cytokines TNF-α, IL-1ß, IL-1RA, IL-6, IL-8, IFN-α, IFN-γ (ELISA); tumor proliferation index was assessed by DNA cytometry. Results were analyzed by Statistica 10.0 program. Results: The dynamics of parameters of the cellular immunity was different in patients of two groups. In group 1, percentage of T lymphocytes in blood decreased (from 66.7±3.3 to 50.4±1.6%), as well as their main subsets (CD4+ and CD8+ cells: from 33.6±2.7 to 27.0±1.7% and from 26.7±2.4 to 20.7±1.7% respectively). Patients of group 2 developed an increase in levels of NK cells from 10.1±1.2 to 15.3±2.2%, and levels of CD3+, CD4+ and CD8+ cells were significantly higher than in group 1: 35.0±1.8% for CD4+ and 28.3±2.9% for CD8+ (p &lt; 0.05). The groups also differed in indices of local immunity: DN cells levels in group 2 were lower than in group 1 (5.8±1.0 vs. 18.4±5.4%) and CD4+ were higher (36.6±3.3 vs. 26.2±3.1%; p &lt; 0.05). Patients of group 2 showed lower levels of IL-1ß, IL-6, IL-10, while IFNγ was elevated by 5.4 times, indicating a more favorable local cytokine status of the patients, compared to group 1. Conclusions: In rectal cancer patients with effect confirmed by DNA cytometry, prolongation of chemoradiotherapy to 6-8 weeks provides the formation of a more favorable immunological microenvironment of the tumor, and in such cases it is considered appropriate.

The alpha-melanocyte-stimulating hormone acts as a local immune homeostasis factor in experimental allergic asthma.

Originally, the neuropeptide α-melanocyte-stimulating hormone (α-MSH) has been described as a mediator of skin pigmentation. However, recent studies have shown that α-MSH is able to modulate inflammation in various tissues including the lung. So far, it is still not clear whether α-MSH also plays a role in allergic bronchial asthma. This study aimed at investigating the role and regulatory mechanisms of α-MSH in asthma pathogenesis. α-MSH levels were measured in bronchoalveolar lavage (BAL) fluid of asthmatic and non-asthmatic individuals as well as of healthy mice and mice with experimental asthma. Wild-type mice were sensitized to ovalbumin (OVA) and exposed to an OVA aerosol in order to induce experimental allergic asthma. α-MSH was administrated intratracheally, the α-MSH antibody intraperitoneally prior each OVA challenge. Airway inflammation, cytokine production, mucus production, airway hyperresponsiveness and receptor expression were assessed. α-MSH levels in BAL of asthmatic individuals and mice were significantly higher compared to healthy controls. In a mouse model of experimental asthma, α-MSH neutralization increased airway inflammation and mucus production, whereas local administration of α-MSH significantly reduced inflammation of the airways. The beneficial effects were further associated with decreased levels of eosinophilic chemoattractant factors that are released by MC5R-positive T helper 2 and airway epithelial cells. α-MSH acts as a regulatory factor to maintain local immune homeostasis in allergic bronchial asthma.

Evaluation of influence several drugs with local antimicrobial activity against local immunity cells

Currently in clinical practice widely used drugs local antimicrobial drugs such as Tantum Verde (benzydamine) Tantum Rosa (benzydamine) Miramistin (benzyldimethyl-miristoilamino-propylammonium) Hexoral (hexetidin), chlorhexidine (chlorhexidine), Septolete total (benzydamine + cetylpyridinium chloride). The mechanism of action of these very similar. We evaluated the effect of these drugs on the viability of lymphoid tissue cells and their effect on the neutrophilic part of the immune system, which are the most important factors of local immunity and, at the same time, part of the immune system which is responsible for innate immunity. We used peripheral blood from 6 healthy donors and 6 patients with inflammatory diseases (abscess of the abdominal cavity). Evaluation of the viability of lymphocytes was performed in a test using trypan blue. The functional state of neutrophils was performed in a nitro-blue tetrazolium test. The final concentration of the studied drugs in all experiments was 10% of the initial recommended for local use. The results of the study showed that Miramistin, Hexoral, Chlorhexidine, Septolete Total cause the death of lymphocytes isolated from healthy donors and patients with severe inflammation process. Tantum Verde and Tantum Rose do not cause the death of lymphocytes. All studied drugs (except Tantum Verde and Tantum Rose in healthy donors) reduce the number of neutrophils containing formazan granules, which indicates the suppression of the activity of the NADPH oxidase system. Patients’ neutrophils witch were activated by inflammatory process under the influence of miramistin experiencing short-term excessive activation of the NADPH-oxidase system, which can lead to tissue damage in severe inflammation.

Effect of esberitox phytopreparation on factors of local immunity of the oral cavity

Introduction: Among a large number of immunomodulation agents, a special role belongs to herbal medicines. They differ in relative harmlessness and high efficiency, both with system and local use. Clinical and immunological efficacy of Esberitox phytopreparation (Schaper&amp;Brummer, Germany) in a number of diseases of viral and microbial origin has been shown. This report presents data on the effect of Esberitox on the factors of local immunity of the oral cavity in patients with chronic pharyngitis, tonsillitis and periodontitis with systemic use of Esberitox in monotherapy. Methods: The oropharyngeal secret of 10 patients with chronic tonsillitis (CT), 11 patients with chronic pharyngitis (CP), (all patients after tonsillectomy), 10 patients with chronic periodontitis was investigated. Patients were given monotherapy with «Esberitox» for 10 days (CP), according to the manufacturer's instructions for use of the drug. In addition, 9 practically healthy persons (K) of the same age (18-50 years) were examined. The ELISA method was used to determine the class A immunoglobulins (secretory and monomeric), IgG, α-interferon, interleukin-1β, levels of immune complexes. Statistics: Mann-Whitney U-test. Results: Studies have shown that in patients with chronic inflammatory diseases of the oral cavity there is a decrease in the protective factors of local immunity – the level of secretory IgA, antiviral factor – α-interferon and an increase in the level of pro-inflammatory cytokine – interleukin-1β, monomeric form of IgA and the number of immune complexes. The use of «Esberitox» as a monotherapy was accompanied by an increase in the concentration of class A secretory immunoglobulin, the «normalization» of the α-interferon content (increase in concentration) and interleukin-1β (decrease in content). Conclusion: The resulting positive effects from the use of esberitox allow us to recommend the drug as part of the complex therapy of chronic diseases of the oropharynx, and in the form of monotherapy.

Does histoid leprosy represent a locally hyperimmune variant of lepromatous leprosy?

Histoid leprosy is a subtype of leprosy with distinct clinical presentation and histologic features. It accounts for <4% of leprosy cases. The exact location of histoid leprosy along the immune spectrum and its relation to other subtypes is unclear. To evaluate the local immune phenomenon which define histoid leprosy. Parallel skin biopsies from histoid lesions and from unremarkable skin in patients with histoid leprosy were evaluated and the histologic findings compared. Formalin fixed, paraffin embedded tissue sections from lesional and non-lesional biopsies were assessed for changes in epidermis and dermis; type and extent of infiltrate, presence or absence of pseudocapsule and associated reactions. Bacillary index was evaluated using Wade Fite stain for lepra bacilli. Amongst 208 leprosy cases, six cases of histoid leprosy were identified (2.88%). The cases showed presence of nodules, patches and plaques overlying clinically unremarkable skin. Fourteen skin biopsies were evaluated of which the lesional biopsies showed equal proportion of fusocellular, fusocellular epithelioid and fusocellular-vacuolated histology. A greater circumscription was noted in lesional biopsies; however the cellular content of the infiltrate was similar in lesional and non-lesional biopsies. A case of erythema nodosum leprosum in histoid leprosy was also seen. Ours is the first study comparing normal and lesional skin in histoid leprosy. Though the histoid lesions appear to have a derivation from lepromatous leprosy, the local histologic and clinical alterations may be a result of heightened local immunity or reactive local modifying factors.

Efficacy of probiotic application in the treatment and prevention of peri-implant mucositis

The aim of this study was to evaluate the effectiveness of probiotics in the treatment and prevention of peri-implant complications. Clinical, immunological and microbiological studies were conducted before, 1 and 6 months after the periodontal treatment in 42 patients (120 implants) with peri-implant mucositis. The use of probiotics in the treatment of peri-implant mucositis led to a significant and more pronounced improvement in oral health state in terms of an increased functional activity of the salivary glands, local immunity factors and microbiocenosis.

The Local Immunity of the Oral Cavity in Women with Recurrent Aphthous Stomatitis Associated with Urogenital Infection

This paper studied the dynamics of local immunity factors of the oral cavity in women with recurrent aphthous stomatitis against the background of urogenital infection. The research proves the maximum efficacy of hydroxyzine hydrochloride, aminodihydrophthalasindione sodium and Eplan used in multiple treatments of recurrent aphthous stomatitis in patients with urogenital infection as evidenced by a marked reduction in the coefficient of local immunity factors balance, which is observed as early as on day 7 of the therapy provided. Moreover, the findings show the growth of immunological parameters of oral fluid (lysozyme and SIgA), the normalization of the coefficient of local immunity factors balance in the treatment of recurrent aphthous stomatitis in women with urogenital infection, the improvement being observed 3 months after the treatment suggesting a favorable local immunity of the oral cavity.

Improving the efficiency of treatment of inflammatory periodontal diseases in professional athletes

Aim. Evaluation of the effectiveness of the medication «Pomegranate balm» in the treatment of inflammatory periodontal diseases in professional athletes. Methods. Among 475 professional athletes the prevalence of periodontal diseases was studied using the Community Periodontal Index of Treatment Needs. Silness-Löe plaque index, periodontal index, Muhlemann sulcus bleeding index modified by I. Cowell and biochemical analyses were determined in 51 freestyle and classical style wrestlers divided into three groups: group 1 - 20 athletes with intact periodontium, group 2 - 16 athletes with chronic catarrhal gingivitis, group 3 - 15 wrestlers with the signs of mild periodontitis. Sialometry was performed, the concentration of secretory immunoglobulin A and interleukin-8 in oral fluid was measured by the method of enzyme linked immunosorbant assay (ELISA). Results. The maximum values of gingival bleeding were recorded in groups 1 and 2 of the examined athletes - 20.9±3.79 and 34.5±3.21%, respectively. The incidence of moderate and severe periodontal diseases in the age group of 24-32 years was in average 14.1±2.35 and 5.5±1.53%, respectively. Use of the drug sharply increased the rate of basic salivation (from 1.98±0.031 to 2.23±0.023 ml/min, p &lt;0.001), and by the end of the investigation, in most of the athletes the earlier observed signs of hyposalivation resolved, and the analysis of laboratory data revealed markedly increased level of secretory immunoglobulin A from 260.2±1.47 to 544.0±6.33 mg×mg-1 protein and decreased levels of interleukin-8 from 2829.6±15.8 to 1224.2±18.0 pg×mg-1 protein (p &lt;0.001). Conclusion. Effective and biologically active preparation based on propolis «Pomegranate balm» can significantly improve the dental status of professional athletes against the background of improving the state of factors of local oral immunity.

Factors of local immunity in esophageal tumors of various stages.

e16013Background: The purpose of the study was a comparative assessment of parameters of the local cellular immunity in patients with esophageal tumors of various stages. Methods: We studied tissue...

Interfaces of Malignant and Immunologic Clonal Dynamics in Ovarian Cancer

High-grade serous ovarian cancer (HGSC) exhibits extensive malignant clonal diversity with widespread but non-random patterns of disease dissemination. We investigated whether local immune microenvironment factors shape tumor progression properties at the interface of tumor-infiltrating lymphocytes (TILs) and cancer cells. Through multi-region study of 212 samples from 38 patients with whole-genome sequencing, immunohistochemistry, histologic image analysis, gene expression profiling, and T and B cell receptor sequencing, we identified three immunologic subtypes across samples and extensive within-patient diversity. Epithelial CD8+ TILs negatively associated with malignant diversity, reflecting immunological pruning of tumor clones inferred by neoantigen depletion, HLA I loss of heterozygosity, and spatial tracking between Tcell and tumor clones. In addition, combinatorial prognostic effects of mutational processes and immune properties were observed, illuminating how specific genomic aberration types associate with immune response and impact survival. We conclude that within-patient spatial immune microenvironment variation shapes intraperitoneal malignant spread, provoking new evolutionary perspectives on HGSC clonal dispersion.

CLINICAL AND LABORATORY EFFICIENCY OF TREATMENT OF WOMEN WITH PERIODONTAL DISEASES ON THE BACKGROUND OF BACTERIAL VAGINOSIS

Introduction. There is currently no tactic for dental examination and treatment of women suffering from bacterial vaginosis (BV), although chronic catarrhal gingivitis, periodontitis of initial and first degree of severity in patients with this pathology has been proven. The frequency of detection of BV in modern women aged 18-45 is 67-89%. The main pathogens of BV are Gardnerella vaginalis and Atopobium vaginae.&#x0D; Aim. To determine clinical and laboratory effectiveness of the treatment scheme for women with periodontal diseases on the background of bacterial vaginosis.&#x0D; Materials and methods. Diagnosis of BV was established by an obstetrician-gynaecologist based on the Amsel criteria. 54 women aged 18-45 were examined. The examined women were divided into two groups, depending on the intended scheme of integrated treatment. Determination of the degree of inflammation of the periodontal period was carried out according to the PMA indices (modified by S. Parma), KPI by Leus. The quality control of the treatment was performed by PCR method, the presence of Gardnerella vaginalis and Atopobium vagina, the amino test of oral liquid, the quantitative activity of alpha amylase and the concentration of sIgA in saliva. Representatives of the first control group (26 women) were prescribed the common course of treatment, namely: local treatment therapy (oral baths of the solution of the medicine “Stomatophyte” after morning and evening hygiene procedures, application of “Mergagil-denta” (7 days); general therapy (metronidazole 250 mg every 8 hours for every 7 days, probiotic “Symbiter acidophilic concentrate” for 21 days). Women of the second experimental group (28 patients) were treated according to the scheme we patented. Their local treatment included: oral baths with the medicine “Stomatophyte” after morning and evening hygiene procedures (7 days), tablets for resuscitation “Lizak” for 1 tab. every 6 hours (5 days), application of “Metrogil-denta” for 20 minutes twice a day. After using “Stomatophyte” (10 days), use “Symbiter omega” for 21 days in silicone caps for the night after the completion of the use of “Metrogil-Denta”. General medicines for patients in the 2nd group: clindamycin 150 mg every 6 hours (7 days), probiotic “Symbiter acidophilic concentrate” per unit (21 days). Evaluation of the treatment results was carried out 14 days, 3, 6 and 12 months after the end of the course treatment.&#x0D; Results. In the examined two groups, there was a disorder in the condition of periodontal tissues. In women of the first group chronic catarrhal gingivitis was registered in 11.5%, chronic generalized periodontitis of initial degree - in 57.69%, chronic generalized periodontitis - in 30.77%; in patients of the 2nd group – in 10.71%, 64.29% and 25% respectively. None of the 54 patients had clinically intact periodontal disease. The PMA and Leish KPI indices in women of the 1st group after the treatment did not have a significant difference with the initial results, while the rates of patients in the second group decreased significantly, indicating a decrease in inflammation in the periodontal tissues. The concentration of sIgA after the end of the treatment course was higher in women of the 2nd group, which suggests a higher efficacy of the proposed method of treatment.&#x0D; Conclusions. Comparing the conventional and patented treatment regimen, it has been determined that traditional treatment is significantly less effective. Clinical methods of examination are insufficient to control the quality of treatment of women with BV and manifestations of periodontal disease, since a specific microflora appears in the oral cavity of this contingent of patients. In order to objectify clinical results, it is advisable to use a study of local immunity factors, such as saliva alpha-amylase and sIgA. To control the presence of IB pathogens as an express method, an amino test should be used, and in the case of its positive result, PCR should be used to detect Gardnerella vaginalis and Atopobium vaginae.

Possibility of predicting the efficiency of soft tissue sarcoma treatment on the basis of features of their immunological microenvironment

Цель. Изучить возможность использования оценки факторов локального клеточного иммунитета для прогнозирования эффективности лечения сарком мягких тканей.&#x0D; Методы. В исследование вошли 38 пациентов с саркомой мягких тканей: 22 с первичными опухолями и 16 с рецидивными, находившихся на хирургическом лечении в 2014-2016 гг. У всех пациентов с целью оценки показателей локального иммунитета исследовали полученные интраоперационно ткани опухоли, перитуморальной зоны и условно здоровые ткани, соответствующие линиям резекции (всего 114 образцов). Образцы тканей гомогенизировали, субпопуляции лимфоцитов определяли на проточном цитометре.&#x0D; Результаты. Исследованы характеристики лимфоцитарного микроокружения сарком мягких тканей в связи с клиническим эффектом комплексного лечения больных. При первичных саркомах развитию у больных бессобытийной выживаемости более 12 мес после операции соответствовало более высокое содержание NK-клеток в ткани опухоли и более высокое соотношение уровней NKT-лимфоцитов в перитуморальной зоне и опухоли, а также более низкий уровень Tregs, чем у больных с бессобытийной выживаемостью менее 12 мес. При рецидивных саркомах короткая бессобытийная выживаемость была связана с высокими показателями уровня DN-клеток в опухоли и соотношения доли CD3+-лимфоцитов в перитуморальной зоне и опухоли. Все указанные различия статистически значимы (р &lt;0,05).&#x0D; Вывод. Клинический эффект комплексного лечения сарком мягких тканей связан с исходным состоянием иммунологического микроокружения опухоли; ряд показателей локального клеточного иммунитета можно рассматривать в качестве прогностических факторов, различных для первичных и рецидивных сарком.

Clinical and immunological features of oral and vermillion border precancer diseases

Research objective was assessment of a possibility of primary diagnosis of viruses Epstein-Burr, a cytomegalovirus, a virus of herpes of the 6th type in oral liquid and also influences of herpes infection on development and the clinical course of precancer diseases of the oral mucosa and the red border of lips (RBL). Profound clinic-immunological examination is conducted to 60 patients: the first group are have made 20 patients with an erosive and ulcer form of the lichen planus, the second - 20 people with an erosive form of a leukoplakia, a third - 20 people without diseases of the oral mucosa. As a result of work detection herpes infection is revealed high (90%). The combined infection with a virus Epstein-Burr and a virus of herpes of the 6th type was observed more than at a half of patients. The imbalance of factors of local immunity of the oral cavity in the form of increase in the IgG profile, decrease in concentration of IGA, lysozyme, and increase in an indicator of Ksb three times in comparison with norm and also substantial increase of level of pro-inflammatory cytokin IL 1β and FNO-α is found in patients with precancer diseases of the oral mucosa. Characteristic clinical feature of the precancer diseases associated with latent herpes infection is the long, recidivous current, persistent to traditional therapy. The research of oral liquid and blood on herpes viruses and consultation of the infectiologist is recommended to all patients with precancer diseases.