Evaluation of influence several drugs with local antimicrobial activity against local immunity cells

Abstract

Currently in clinical practice widely used drugs local antimicrobial drugs such as Tantum Verde (benzydamine) Tantum Rosa (benzydamine) Miramistin (benzyldimethyl-miristoilamino-propylammonium) Hexoral (hexetidin), chlorhexidine (chlorhexidine), Septolete total (benzydamine + cetylpyridinium chloride). The mechanism of action of these very similar. We evaluated the effect of these drugs on the viability of lymphoid tissue cells and their effect on the neutrophilic part of the immune system, which are the most important factors of local immunity and, at the same time, part of the immune system which is responsible for innate immunity. We used peripheral blood from 6 healthy donors and 6 patients with inflammatory diseases (abscess of the abdominal cavity). Evaluation of the viability of lymphocytes was performed in a test using trypan blue. The functional state of neutrophils was performed in a nitro-blue tetrazolium test. The final concentration of the studied drugs in all experiments was 10% of the initial recommended for local use. The results of the study showed that Miramistin, Hexoral, Chlorhexidine, Septolete Total cause the death of lymphocytes isolated from healthy donors and patients with severe inflammation process. Tantum Verde and Tantum Rose do not cause the death of lymphocytes. All studied drugs (except Tantum Verde and Tantum Rose in healthy donors) reduce the number of neutrophils containing formazan granules, which indicates the suppression of the activity of the NADPH oxidase system. Patients’ neutrophils witch were activated by inflammatory process under the influence of miramistin experiencing short-term excessive activation of the NADPH-oxidase system, which can lead to tissue damage in severe inflammation.