Local Cows Research Articles

Assessing the Potential Diagnostic Value of Indonesian Local Allergen Skin Prick Testing (SPT) for Cow’s Milk Allergy among Atopic Dermatitis Patients

Background: Atopic dermatitis (AD) is a multifactorial chronic, frequently recurrent, inflammatory skin condition. The development of IgE-mediated food allergies and food sensitivity are both associated with atopic dermatitis. Cow’s milk allergy (CMA) caused the most common hypersensitivity reaction during childhood; however, the prevalence in adults is around 0.5%. Patients with AD use the Skin Prick Test (SPT) to evaluate the specific sensitization process. Purpose: This study aimed to assess cow's milk allergy among adult AD patients using local allergen extract SPT, standard SPT, and specific IgE (sIgE), as well as evaluating the conformity and relevance of the test results. Methods: Using consecutive sampling technique, the study was conducted twice on 45 adult AD patients with a one-week interval between administrations. Result: Local cow's milk SPT showed 4 positive results, and standard SPT showed 5. No sIgE test was positive. Local SPT was negative for 86.67% of individuals without a history of suspected cow's milk allergies. Standard SPT results were positive for 2.22% of individuals with a history of suspected cow's milk allergies and negative for 86.67% of those without. The relevance between local and standard SPT was shown to be substantial (k = 0.384, p = 0.000). Conclusion: The result of the local cow’s milk SPT and the patient’s history had good conformity, and the relevance with standard SPT was significant in diagnosing cow’s milk allergy among AD patients.

Economics of Milk Production of Local Cows in Bhagalpur District of Bihar

"Given that most Indians consume vegetarian food, their demand for dairy products has been growing gradually and is anticipated to do so in the future. A list was made based on the cumulative square root and the size group of milk producers, namely small, medium, and large, in order to distinguish between Dairy Cooperative Society (DCS and Non-DCS) populations. The sample size was calculated using the Taro Yamane method to be 278 samples from 912 milk producer populations in the chosen six villages of each block. The overall farm costs C were estimated to be ` 36.28 thousand, with the small farm ` 36.50 thousand spending the most, followed by the medium farm spending in ` 36.47 and the large farm spending of ` 33.86 thousand. The overall farm cost of milk production was estimated to be 29.17 per Kg, which varied from ` 33.25 per Kg of small farms to ` 32.15 per Kg of medium farms to ` 28.47 per Kg of large farms. The average gross return per cow for the overall farm was in ` 44.85 thousand per year, which varied from ` 44.23 thousand for small farms to ` 45.86 thousand for medium farms to ` 48.58 thousand for large farms."

Enhancing irrigated forage crop production through water and nutrient management in the Ethiopian sub-humid highlands

IntroductionThe increasing pressure on land and water resources, fueled by high population growth and climate change, has profound implications for crop yield and quality. While studies thrive for various crops, a notable research gap exists in understanding the responses of forage crops to irrigation and nutrient management in developing countries. This study aims to address this gap by assessing the impact of irrigation and fertilizer application on forage production in the Ethiopian sub-humid highlands.MethodsThe experiment focused on four forage varieties, namely Napier grass (Cenchrus purpureus) cultivars, ILRI-16791, ILRI-16819, ILRI-16803, and Guinea grass (Megathyrsus maximus) ILRI-144 cultivated in experimental plots. Three irrigation levels designated as IR60 (60% of total available soil water), IR80 (80%), and IR100 (100%) were applied, along with three fertilizer rates: organic manure at 30 t ha−1, and Urea-N at 100 kg ha−1 and 300 kg ha−1. Agronomic data including growth performance, forage dry matter yield, and nutritional quality were collected during two trial years.Results and discussionAmong the various irrigation treatments, IR80 demonstrated the most favorable balance between forage yield, WUE, net benefit, and LWP. In addition, the highest DMY, WUE, net benefit, and LWP were obtained for UREA at the rate of 300 kg ha−1 while the lowest DMY and WUE were observed for UREA at the rate of 100 kg ha−1. Significant variations were observed among the four forage varieties, with Napier grass ILRI-16791 having the highest DMY (9.8 tons ha−1), WUE (39 kg ha−1 mm−1), LWP (0.28 USD m−3 for local cows, and 1.04 USD m−3 for crossbred cows), and net benefit (783 USD ha−1). For all forages combined, a 40 and 20% decrease in irrigation increased water use efficiency by 17 and 9.4%, respectively. These results indicate that a moderate level of deficient irrigation such as IR80 could be a viable water management strategy for irrigated forage, especially in water-scarce areas. The conserved water saved from the deficit irrigation can thus be used to irrigate additional land, contributing to a more sustainable and efficient water usage approach.

Polymorphism of the DGAT1 gene and its relationship to milk yield and some chemical properties of milk during the first stage of production in local cows and Holstein cows; a comparative study

In present study, 50 dairy cows of the local and Holstein breeds were used (34 local cows and 16 Holsteins). Milk samples were collected from 50 cows once every two weeks from the morning milking (to estimate the daily amount of milk, multiply the amount by 2). One-day test to calculate the daily and monthly milk yield. Samples were taken at a rate of 50 ml for each cow, and the proportions of the main milk components (fat, protein, lactose and non-fat solids) were estimated. Blood samples were drawn from cows for the purpose of DNA extraction. Then the DGAT1 gene was amplified using the primers of the studied piece, which was 411 bp in size. The gene was cut using Taq1 restriction enzyme. The results showed the presence of three genotypes: AA, AK, and KK. In terms of the quantity of daily, weekly, monthly, and first stage milk production, the findings also demonstrated that the genotypes AK and KK of the Holstein breed were superior (P< 0.05) than the two genotypes AA and AK of the local breed. While the genotype AA of the local breed was superior to the genotype AK in terms of daily, weekly, and monthly milk production as well as the first stage, it was also discovered that there is a correlation between the genotypes and the chemical components of milk, as the results showed that the genotype AA was significantly superior (P<0.05) by the percentage of fat in the local breed on the AK and KK genotypes of both breeds.

Conception rates of cows inseminated with frozen semen of Rural Development Academy, Bogura, Bangladesh

Background: The first service conception rate (FSCR) is an important parameter for any artificial insemination (AI) programme. The present study was conducted to determine the FSCR and its influencing factors in cows inseminated with frozen semen produced by Rural Development Academy, Bogura. Methods: Data were collected from 372 cows at 39 villages under Sherpur Upazilla of Bogura district on breed, age, body condition score (BCS), parity, milk yield, suckling, AI season and the interval between oestrus to AI using a questionnaire. Results: The overall FSCR of cows was 66.4% and the service per conception was 1.53. The FSCR was higher (74.1%) in local cows compared to the Holstein-Friesian (HF) cross counterpart (64.2%). The FSCR was higher (70.4%) in 24-47 months old cows compared to 84-98 months old counterpart (61.5%). The highest FSCR (81.6%) was observed in cows with 3.0 to 3.5 BCS and the lowest (25.7%) was observed in cows with 2.5 BCS. The highest FSCR (68.3%) was observed in the 0 (zero) parity (heifers) group of cows and the lowest (62.5%) was observed in the 4-7 parity counterpart. The highest FSCR (72.4%) was observed in cows yielded daily >15-22 L milk and the lowest (62.1%) was observed in cows yielded ≤5 L milk. The highest FSCR (72.7%) was observed in suckling restricted cows and the lowest (60.9%) was observed in cows that had several times suckling daily. The highest FSCR (72.1%) was observed in cows received inseminations in Rainy (July-October) season and the lowest (58.4%) was observed in cows received inseminations in Winter (November-February). The highest FSCR (67.1%) was observed in cows received inseminations between 12-18 hrs of detection of oestrus and the lowest (59.1%) was observed in cows received insemination between 8-11 hrs of detection of oestrus. However, the difference in FSCR in cows with different BCS groups was significant (p<0.01). Conclusion: The current FSCR in cows can be considered to be satisfactory. The BCS of cows significantly influences the FSCR in cows.

Evaluation of the Physicochemical Properties of Different Imported Milk Brands Offered in Afghanistan Markets

Milk is an enriched food that humans widely consume. It is the source of many nutrients like protein, fat, carbohydrates, vitamins, and minerals. The content of high-quality milk should match that of natural milk and national and international standards. In the current study, the quality of selected imported milk brands (Pak1, Pak2, Pak3, Pak4, and Ir1) and local cow milk in the markets of Kabul City was evaluated. For this purpose, the levels of protein, fat, total solid, solid not fat, acidity, pH, and specific gravity were measured. Data analysis was conducted using the Kruskal-Wallis test in GraphPad Prism software. Our findings showed that the protein level in imported brands was significantly lower than the standard of cow milk (p<0.01). Among imported brands, the protein level in Ir1 and Pak4 was higher than other brands and the standard (p<0.05). The level of fat in Pak2 and Pak3 was higher than the standard and other brands (p<0.05), the level of total solid in Ir1 was lower than the standard (p<0.05), and the level of solid not fat in Pak2 and Pak3 was lower than the standard (p<0.05). Our results showed that local cow milk has a higher quality than imported milk brands, and among the brands, Pak4 has a higher quality than other brands, where its quality is almost the same as the composition of local cow milk.

Molecular Characterization of Vigna unguiculata (L.) Walp Collection based on ISSR Markers

Background: In Azerbaijan, cow pea is one of the fundamental legumes. This study was carried out at the Institute of Genetic Resources, Azerbaijan National Academy of Sciences to evaluate genetic diversity in 28 cowpea accessions from Azerbaijan national gene bank. The aim of this study was to evaluate the genotypic diversity, relatedness and population structure among local cow pea resources available in Azerbaijan and the cultivated VIR accessions based on ISSR markers. Methods: The studies were conducted in 2017-2019 at the Institute of Genetic Resources (IGR) of the National Academy of Sciences (NAS) of Azerbaijan. 28 samples were used as research material: 10 of them were local forms and 18 were samples obtained from VIR. Sowing of collection samples was carried out in duplicate with an area of food of one plant 10 ´ 45 cm at the optimum time, in the at the end of April. Result: In the study, 28 alleles were synthesized for the collection with five ISSR markers, with an average polymorphism index of 75%. The rich genetic diversity (GMO = 0.80; PIC = 0.34) was found in the collection. Statistical analyzes have shown that the efficiency of the UBC 835 and the UBC 818 premieres was high. The average genetic distance index in the cow pea collection was 0.33. 71% of the samples were genetically identifiable and 29% revealed that the genotype was identical across the studied loci. PCoA and cluster analysis identified 3 genetically distinct groups within the collection, with the first two coordinates explaining 57% of this genetic variation.

REVIEW ON FERTILITY PERFORMANCES OF LOCAL AND CROSSES OF EXOTIC BREED DAIRY CATTLE IN ETHIOPIA.

Review was carried out on fertility or reproductive performances of Ethiopian locals’ cattle and their Friesian and Jersey crosses. The objectives were to review research results on age of puberty, age at first calving, days open, postpartum anestrus interval, Service period, Number of Services per Conception and calving interval. The available review result showed that F1 Jersey and Friesian crosses had 7.65 and 5.89months shorter AFC than their F2 counterparts, respectively. Likewise, these crosses also had 12.63 and 11.35-months shorter AFC than that of local cows, respectively. Crossbred cows having 75% Friesian inheritance had comparable age at first calving with that of F1 Friesian and Jersey crosses. F1 Friesian crosseswere observed to have 37.67 and 33.96 days shorter calving interval as compared to crosses with 75% Frisian inheritances and F2 Friesian crosses, respectively. F1 Friesian and Jersey crosseshad 33.96 and 54.91 days shorter calving interval than their F2 counterparts, respectively. Crossbred cows having 75% Jersey had better reproductive performances than that of other crosses in reproductive traits considered except in age at first calving. Therefore, if the reproductive performance of local cows is to be improved, cows must be genetically upgraded to the level 50% exotic inheritances (Friesian/Jersey). Upgrading local cattle up to 75% exotic inheritance could also be done for better reproductive efficiency provided that the level of management is good enough to meet the relatively higher managerial demand of these animals. F1 crossbred dairy cattle is suitable to be used as dairy cattle under small holder management conditions and crossbred having 75% exotic blood level for urban and peri-urban dairy producers.

The exploration of indigenous plant-based coagulant for sumbawanese cheese production as probiotic source

Sumbawa cow's milk processing is still limited even though it has the potential to be developed. Therefore, a diversification product like cheese as a source of probiotic is required. Cheese derived from milk that is coagulated using rennet which is expensive and has high halal critical point. Thus, an alternative coagulant that is affordable with low halal critical point such as local plant-based material is needed. This study used Sumbawa local cow’s milk to produce cheese which coagulated using pineapple and gooseberry and the addition of Lactobacillus lactis as the lactic acid bacteria. Observed parameters were clotting time, yield, hardness, protein, total fat, total lactic acid bacteria, and sensory quality. This experiment showed that the cheese yielded using pineapple was higher than that of gooseberry, while the cheese coagulated using gooseberry (26714 N/m2) was having firmer texture. Meanwhile, clotting time, protein content and total fat content of the cheese coagulated using both coagulants were the same. The total lactic acid bacteria of the cheese coagulated using pineapple and gooseberry was similar 10.80 Log CFU/mg and 10.84 Log CFU/mg, respectively. Sensory evaluation using quantitative descriptive analysis showed that the cheese coagulated using pineapple extract has higher aftertaste and bitter taste. Meanwhile, the cheese coagulated using gooseberry extract was dry, grainy, yellowish, and its overall acceptance was higher than that of pineapple.

A REVIEW ON EFFICIENCY OF ARTIFICIAL INSEMINATION IN CATTLE BREEDING IN ETHIOPIA

Ethiopia has large cattle population in Africa. The output of decades of crossbreeding programme in Ethiopia through AI service was quite insignificant because the exotic breeds and their crossbreds of the country accounted for about 1.44%. The objective of this review is to present the efficiency of Artificial Insemination (AI) service in cattle breeding in Ethiopia. The optimum recommended mean number of services per conception (NSC) for profitable dairy cow is about 1.4. However, conventional cattle AI breeding indicated that the mean NSC of different studies ranged from 1.14 in local cows up to 2.47 in different genotypes of cows kept under different management systems and conception rate at first insemination (CR1) ranged from 7.14% to 75.5% in different genotypes of dairy cows kept under extensive and intensive management systems. Estrus synchronization followed AI breeding indicated that CR1 ranged from 24.69% to 70.6% in Zebu cows kept under semi-intensive management system. Calving rate (CR) is the most appropriate measure of fertility of dairy cows which is defined as the number of calves born per 100 services. The poor efficiency of the country cattle AI service is a huge biological economic loss in cattle production and managerial monetary losses. Strategic interventions on cattle AI service efficiency improvement options should be identified and practiced considering breed type, production system and agro-ecology.

Suitability of substrate and vegetation for tropical green roofs

This study investigates the performance of green roofs with three different types of substrate, which are topsoil, a mix of 60% topsoil and 40% biochar, and a mix of 60% topsoil and 40% cocopeat in terms of saturated bulk density and water retention efficiency with a roof slope of 16.7°. The substrate has a thickness of 35 mm and was supported by a timber green roof prototype with Zoysia Japonica, Local cow grass and 9 o’clock flower as vegetation. It was also observed from the experiment that with respect to vegetation, the water retention efficiency is 50.3% (Zoysia Japonica), 49.3% (Local cow grass), and 57.4% (9 o’clock flower) with topsoil and cocopeat for a rainfall of 10 mm for a period of 20 min. Based on this research, a 9 o’clock flower with substrate combining topsoil and coco peat is recommended for green roofs in tropical climates.

Deceitful Catchment Area for the Development of Agriculture and Horticulture Aspects in the Himalayan Region

The glaciers and annual snowfalls in the Himalayan region feed rivers serving 47% of the world’s population. No other region in the world has the comparable number of people, scarcity of rainfall, dependence on agriculture, tempting sites for mega-projects and vulnerability to climate change. Being a part of north-western Himalayan region, the catchment development (CD) block of Banjar in the district Kullu of Himachal Pradesh has 49 Micro Watersheds (MWs) out of which 4 are snow bound and are inaccessible to carry out any developmental activity. In order to do socio-economic study and assess the extent of Horticultural, Agricultural, Vegetable Production and Animal Husbandry development, NERIL conducted field survey in all these Community Development Blocks in 2012. For the purpose of this survey, the random sampling size comprised of minimum 10% of the revenue villages and 5 nos. of households up to a total of 250 households. In case of households exceeding 250 nos. an additional household was surveyed for an increase of every 50 households thereafter. As per these criterion 177 households in CD Block Kullu. The block has 10858 ha area owned by the farmers in which various agricultural activities including vegetable cultivation and animal husbandry are carried out. Additionally, 7254 ha area is available for the cultivation of different fruit crops viz. apple, plum, pomegranate, peanut, persimmon and walnut. The Block has 6935 ha area on which various cereal and vegetable crops are grown. Among the cereal crops, Maize dominates during Kharif season whereas Wheat and Barley are the main crops during Rabi season. Themaize accounts for 84.2% of the cropped area during Kharif season whereas wheat accounts for 82.2 % of the cropped area during Rabi season. The vegetables are grown on 1360 ha (cropped) area in the block. The most preferred vegetable crops are peas, garlic, cauliflower and cabbage. They are grown by 60.7, 45.6, 24.4 and 11.2 % farmers respectively. The farmer’s feed good amount (3.5 kg) of concentrate per cattle per day.The average milk yield of improved breeds is 5.6 liter per day whereas that of local cows is about 2.7 liter perday. The most discouraging feature of the animal husbandry activity has been negligible availability of green fodder. On the above survey data were concluded that, a total sum of Rs. 592.52 lacs will be required to bring the desired improvement in the economy of farmers at least partially and conserve the soil and water to reduce the silt load of the river water system in near future.

Effect of Liquid to Solid Ratio in Rumen Content on Feedstuffs’ in Vitro Fermentability and Digestibility

The amount of fibrous feed consumed by tropical cattle has an impact on the rumen’s liquid-to-solid ratio and feed passage rate. Feed fermentability and digestibility are influenced by ratio. Several in vitro procedures use varying liquid-to-solid ratios, which may or may not be applicable to the local situation. The purpose of this study is to compare the local cow rumen conditions with the liquid-to-solid ratio employed in the in vitro investigation. There were two experiments done for the study. First, the liquid-to-solid ratios in various in vitro experiments (Tilley and Terry = T1, Theodorou = T2, and Sutardi = T3) were compared to the circumstances in the rumen of local cattle (T0). Second, it was determined whether the various liquid-to-solid ratios used in various in vitro techniques were similar in terms of the feedstuffs’ fermentability (pH, NH3, VFA), digestibility (DMD and OMD), and fermentability (DMD and OMD) (F1 = Napier grass, F2 = maize, and F3 = soybean oil meal). The first experiment was fully utilized, and a 3 x 3 block factorial randomized design was used (second experiment). The liquid-to-solid ratio of T3 (1: 40) was discovered to be more comparable to the rumen state of nearby cattle (1:46). Different in vitro techniques had no discernible impact on pH, VFA, DMD, or OMD, but T3 produced more NH3 than T1 or T2 did. With F3 higher than F2 or F1, the major effects of fermentability and digestibility were discovered inside the feed. It is found that every feed studied produced higher NH3 concentrations thanks to the Sutardi method’s liquid-to-solid ratio, which is more akin to the rumen of local cattle. As a result, it is advised to evaluate feedstuff fermentability and digestibility for local cattle using the Sutardi method.

Influence of selected lactic acid bacteria on the sensory characteristics of soft cheese with natural rind

Cheese is obtained by the coagulation of milk, followed by maturation under the action of microorganisms which are responsible for the development of physicochemical and organoleptic characteristics of cheese. Among various microorganisms, lactic acid bacteria (LAB) play an essential role in establishing sensory characteristics of the cheese. In Cameroon, the effect of isolated LAB on the sensory characteristics of cheese produced from local cow milk has not yet been investigated. Therefore, the present work aimed to isolate LAB and evaluate their influence on the physicochemical and sensory characteristics of locally produced soft cheeses. Four strains of LAB were isolated and identified as Enterococcus sp.1, Enterococcus sp.2, Pediococcus sp., and Leuconostoc sp. These isolates were combined in batches for production of cheeses, including Frm 741 (Enterococcus sp.1 and Enterococcus sp.2), Frm 891 (Enterococcus sp.2 and Leuconostoc sp.), Frm 683 (Pediococcus sp. and Enterococcus sp.1), Frm 425 (Pediococcus sp. and Leuconostoc sp.), Frm 503 (Pediococcus sp. and Enterococcus sp.2), Frm 439 (Leuconostoc sp. and Enterococcus sp.1), and Frm 625 (Enterococcus sp.1, Enterococcus sp.2, Leuconostoc sp., and Pediococcus sp.). Cheese produced from the combination of Enterococcus sp.1 and Enterococcus sp.2 (Frm 741), unlike the others, did not drain properly throughout the production process. However, all products showed excellent microbiological quality in relation to biological contaminants, such as Salmonella, staphylococci, and coliforms. Moreover, cheese Frm 425 was most appreciated by consumers for its sweet taste, yellow colour, pleasant smell, and low acidity (51.7°D). On the other hand, cheese Frm 439 was least appreciated by consumers owing to its bad odour, bitter taste, and high acidity (144.9°D). Taken together, the present work demonstrated that the isolated LAB could be used to produce soft cheese with a natural rind and unique sensory characteristics that are appreciated by Cameroonian consumers.

Fatty acid profile of cows milk from regions of Maopolska available on the Polish market

The aim of the study was to compare the percentage of fatty acids in market milk from producers in different regions of Maopolska, during the summer and winter seasons. The study was conducted on pasteurized milk with a fat content of 3.2%, produced in six dairies in the Maopolskie voivodeship. The products were labelled as milk from mountainous areas (M1, M2, M3 and M4), and milk from other areas (M5 and M6). The fatty acid profile was determined using a gas chromatograph. The analysis included saturated fatty acids (SFA), unsaturated fatty acids (UFA), including monounsaturated (MUFA) and polyunsaturated (PUFA), the content of hypocholesterolemic fatty acids (DFA) and hypercholesterolemic fatty acids (OFA), and gave the DFA/OFA and UFA/SFA fatty acid ratios. The results indicate that drinking milk produced from raw material from local cows grazing in mountainous regions had a favourable health-promoting fatty acid profile. It contained more PUFA n-3 acids, including -linolenic acid C18:3 n-3, as well as vaccenic acid C18:1 n-7 and CLA compared to milk from cows from other regions of Maopolska. Milk from cows from mountainous regions also had significantly higher (P<0.05) DFA content and DFA/OFA ratios. Higher levels of desirable DFA acids and DFA/OFA ratios were found in drinking milk during the summer season.

DIETARY DICALCIUM PHOSPHATE SUPPLEMENTATION ENHANCES PRODUCTIVE AND REPRODUCTIVE PERFORMANCES OF CROSSBRED AND LOCAL DAIRY COWS

This study was carried out to investigate the productive and reproductive performances following oral supplementation of dicalcium phosphate (DCP) in different crossbred and local dairy cows. A total of 64 dairy cows of different genetics were selected for the study. All the cows were divided into four different groups and each group consists of 16 cows, namely Local (L), Local ×Holstein Friesian (L×HF), Local × Sindhi (L×S) and Local ×Sahiwal (L×SA). Each group of dairy cows were again subdivided into two subgroups like control and DCP supplement group. We assessed the productive performances such as milk production and lactation length, as well as reproductive performances such as service per conception, post-partum heat period and calving intervals among different breeds of cows as well as in the matching DCP supplement group. The productive performance among the different groups of cows i.e. L×HF, L×S, L×SA and Local cows were found significantly (p<0.01) different. Interestingly, DCP supplement group showed a significant increase of productive performances compared with the control group. Likewise, reproductive performance such as service per conception in different crossbred and Local cows also decreased significantly (p<0.01) with DCP supplement group than the control. Contrarily, the post-partum heat period and calving intervals in different crossbred and Local cows were slightly lower in the DCP supplemented group than control that did not differ significantly. Therefore, the study reveals that L×HF crossbred were found to be better performer followed by L×SA, L×S and Local cows, and DCP supplementation improve all the parameters studied irrespective of genetic variation.

Evaluation Succes Of Program Artificial Insemination On Local Cattle Female In Kuala District Sumatera Utara Province

The purpose of this study was to analyze the success of the artificial insemination (AI) program in local cattle in Kuala District, Langkat, Sumatera Utara Province. It was conducted in five villages: Bekiung, Beruam, Blankahan, Lau Kersik dan Raja Tengah. The survey method was employed in this study. The respondents were selected by using purposive sampling method. There were 41 breeders and 3 inseminators included as the respondents. To be involved in the study, the breeders had to meet certain criteria, such as having an experience of breeding local cows, at least twice using the AI system. As for the inseminators, they were required to have a permit from the Langkat Livestock Service. The results showed that the conception rate (CR) was 71.88%; the service per conception (S/C) was 1.2; the calving interval (CI) was 12.6 months, and the calving rate (CvR) was 96,66%. This study concluded that the implementation of the artificial insemination program in Kuala, Langkat, has been running effectively.

Cervical opening during estrus in Aceh Local Cows based on camera-equipped artificial insemination endoscope

Twelve healthy and not pregnant local cows, aged 3-6 years, 1-3 parity. The cows were synchronized using the PGF2α of 5 ml/cow intramuscularly, twice at 11 days intervals. The Estrus detection based on the scoring of van Eerdenburg was conducted 24 hours after the second prostaglandin injection three times daily for three days. The changes in cervical dilatation were observed at 0, 6, and 12 hours from the onset of estrus. The result showed that 8 of 12 cows were estrus after twice PGF2α injection. All the estrus cows showed mild cervical dilatation with liquid and transparent mucus (opening cervix score 1) at 0-hour observation. Six hours later, 62.50% of cows remained at opening cervix score 1, and 37.50% changed to opening cervix score 2 (optimum cervical dilatation with thick and transparent mucus). At 12 hours after the onset of estrus, 25% of estrus cows had cervical closure (opening cervix score 0, closed cervical with thick and turbid mucus), and 75% changed to opening cervix score 2. In this study, 75% of the cows experienced an optimum open cervix at 12 hours from the onset of estrus. It could be concluded that opening the external cervical canal of local Aceh cows reaches optimum at 12 hours from the onset of estrus.

Cervical opening during estrus in Aceh Local Cows based on camera-equipped artificial insemination endoscope

Twelve healthy and not pregnant local cows, aged 3-6 years, 1-3 parity. The cows were synchronized using the PGF2α of 5 ml/cow intramuscularly, twice at 11 days intervals. The Estrus detection based on the scoring of van Eerdenburg was conducted 24 hours after the second prostaglandin injection three times daily for three days. The changes in cervical dilatation were observed at 0, 6, and 12 hours from the onset of estrus. The result showed that 8 of 12 cows were estrus after twice PGF2α injection. All the estrus cows showed mild cervical dilatation with liquid and transparent mucus (opening cervix score 1) at 0-hour observation. Six hours later, 62.50% of cows remained at opening cervix score 1, and 37.50% changed to opening cervix score 2 (optimum cervical dilatation with thick and transparent mucus). At 12 hours after the onset of estrus, 25% of estrus cows had cervical closure (opening cervix score 0, closed cervical with thick and turbid mucus), and 75% changed to opening cervix score 2. In this study, 75% of the cows experienced an optimum open cervix at 12 hours from the onset of estrus. It could be concluded that opening the external cervical canal of local Aceh cows reaches optimum at 12 hours from the onset of estrus.

Effect of level of Interleukin 1 beta (Il-1B) and Luteinizing hormone (LH) impacts on the size of the ovarian follicles of local cows

This study was conducted in the laboratories of the Department of Animal Production Techniques at the Technical College in Mussaib (TCM) from September 2021 to January 2022 to study the relationship between Interleukin 1 beta (il-1B) and Luteinizing hormone (LH) with ovarian follicle size on local cows. 150 reproductive systems were collected from non-pregnant local cows, which slaughtered in the abattoirs of Babylon Province and were transported to the laboratory within two hours. Ovaries were removed (300 ovaries) and their dimensions were measured. The follicles were measured and classified into three categories. Follicular fluid was drawn from the visible follicles (2-8) on the surface of each ovary and placed in an Eppendorf tube to measure the concentration level of Interleukin 1 beta (il-1B) and Luteinizing hormone (LH) in the follicular fluid. The results showed a significant high superiority (P<0.01) of an increase in the level of interleukin-1beta over the size of large, medium and small follicles. A significant high effect (P<0.01) of increasing the level of the ovulation hormone on follicle size was observed for all small, medium and large groups. We conclude from this study that the concentrations of cytokines (interleukin-1beta) and hormones (Luteinizing hormone) are influenced by the size of the ovarian follicle.