Live 3D Research Articles

Light-sheet autofluorescence lifetime imaging with a single-photon avalanche diode array.

Fluorescence lifetime imaging microscopy (FLIM) of the metabolic co-enzyme nicotinamide adenine dinucleotide (phosphate) [NAD(P)H] is a popular method to monitor single-cell metabolism within unperturbed, living 3D systems. However, FLIM of NAD(P)H has not been performed in a light-sheet geometry, which is advantageous for rapid imaging of cells within live 3D samples. We aim to design, validate, and demonstrate a proof-of-concept light-sheet system for NAD(P)H FLIM. A single-photon avalanche diode camera was integrated into a light-sheet microscope to achieve optical sectioning and limit out-of-focus contributions for NAD(P)H FLIM of single cells. An NAD(P)H light-sheet FLIM system was built and validated with fluorescence lifetime standards and with time-course imaging of metabolic perturbations in pancreas cancer cells with 10s integration times. NAD(P)H light-sheet FLIM in vivo was demonstrated with live neutrophil imaging in a larval zebrafish tail wound also with 10s integration times. Finally, the theoretical and practical imaging speeds for NAD(P)H FLIM were compared across laser scanning and light-sheet geometries, indicating a to acquisition speed advantage for the light sheet compared to the laser scanning geometry. FLIM of NAD(P)H is feasible in a light-sheet geometry and is attractive for 3D live cell imaging applications, such as monitoring immune cell metabolism and migration within an organism.

On-chip clearing for live imaging of 3D cell cultures.

Three-dimensional (3D) cell cultures provide an important model for various biological studies by bridging the gap between two-dimensional (2D) cell cultures and animal tissues. Microfluidics has recently provided controllable platforms for handling and analyzing 3D cell cultures. However, on-chip imaging of 3D cell cultures within microfluidic devices is hindered by the inherent high scattering of 3D tissues. Tissue optical clearing techniques have been used to address this concern but remain limited to fixed samples. As such, there is still a need for an on-chip clearing method for imaging live 3D cell cultures. Here, to achieve on-chip clearing for live imaging of 3D cell cultures, we conceived a simple microfluidic device by integrating a U-shaped concave for culture, parallel channels with micropillars, and differentiated surface treatment to enable on-chip 3D cell culture, clearing, and live imaging with minimal disturbance. The on-chip tissue clearing increased the imaging performance of live 3D spheroids with no influence on cell viability or spheroid proliferation and demonstrated robust compatibility with several commonly used cell probes. It allowed dynamic tracking of lysosomes in live tumor spheroids and enabled quantitative analysis of their motility in the deeper layer. Our proposed method of on-chip clearing for live imaging of 3D cell cultures provides an alternative for dynamic monitoring of deep tissue on a microfluidic device and has the potential to be used in 3D culture-based assays for high-throughput applications.

Acoustic prison for single live cell 3D multi-imaging enabled by light-sheet microscopy

Having a system capable of obtaining high resolution, quantitative, subcellular information of individual live cells through imaging, while minimizing cell damage, is of great importance in many fields of research. In recent years, optofluidic light-sheet fluorescent microscopy (LSFM) has emerged as a powerful tool providing a low photo-toxic imaging method utilising the fluidic environment offered by microfluidics. Here, the benefits of LSFM were integrated with an acoustic single cell prison for precise single cell handling and 3D multi-imaging in a semi-automated manner. The compact, monolithic, acousto-optofluidic platform employed standing surface acoustic waves (SSAWs) to sequentially trap an individual cell on either side of an imaging region, which gathered planar, cross-sectional images of the cell. A cytoplasmic stain was utilised to not only visualize the cell trajectory throughout the imaging process, but also to verify the cell viability post-acoustic exposure. A mitochondrial stain was also used to better demonstrate the resolution capabilities of the device. Through post-image processing, 3D volumetric images of the cell were reconstructed and the results between the first and second rounds of imaging were directly comparable. Furthermore, the acoustic prison advantageously positions the cells in the upper region of the channel, enabling the ability to accurately compare temporal changes in cell morphology; a capability that can lead to advancements in therapeutics and drug delivery to access responses of cells to stimuli over time.

Future of virtual education and telementoring

To summarize recent technological advancements in medical and surgical education and explore what the future of medicine might be as it relates to blockchain technology, the metaverse, and web3. Through the use of digitally assisted ophthalmic surgery and high dynamic range 3D cameras, it is now possible to record and live stream 3D video content. Although the 'metaverse' is still in its early stages, there are a variety of proto-metaverse technologies that exist to facilitate user interactions that can mimic the real world through the use of shared digital environments and 3D spatial audio. Advanced blockchain technologies can allow for further development of interoperable virtual worlds where a user has an on-chain identity, credentials, data, assets, and much more that they can carry across platforms seamlessly. As remote real-time communication becomes an integral part of human interaction, 3D live streaming has the potential to revolutionize ophthalmic education by removing traditional geographic and physical constraints of in-person surgical viewing. The incorporation of metaverse and web3 technologies has created new outlets for knowledge sharing that may improve how we operate, teach, learn, and transfer knowledge.

Abstract 6613: Label-free multimodal multiphoton microscopy for predicting DNA damage response in patient derived non-small cell lung cancer organoids

Abstract Background: DNA damage response (DDR) is a fundamental mechanism for evaluating efficacy of cancer therapeutics, many of which target DNA or associated processes (e.g., etoposide: inhibits DNA synthesis, cisplatin: crosslinks DNA). Current techniques to evaluate DDR rely on immunostaining for gamma H2AX foci (γH2AX), which is an indicator of DNA double-strand break formation. While γH2AX immunostaining can provide a snapshot of DDR in fixed cellular and tissue samples, this method is technically cumbersome as: 1) temporal monitoring of DDR requires timepoint replicates; 2) extensive assay development efforts for 3D samples such as organoids, and 3) protocols for γH2AX immunostaining and its evaluation are time-consuming. The goal of this project is to reduce overall burden on assay duration and development in NSCLC organoids by leveraging label-free multiphoton imaging. Method: To predict DDR in 3D organoid samples, label-free multimodal multiphoton techniques such as simultaneous label-free autofluorescence multiharmonic (SLAM) imaging can provide intracellular information based on endogenous contrast mechanisms. SLAM makes use of a single laser to excite autofluorescence in multiple cellular components including FAD and NAD(P)H, enabling the calculation of the optical redox ratio, which estimates the equilibrium of electron donating versus accepting species in cells. Moreover, label-free, multiphoton systems enable imaging of live samples without the need to add labels or generate sacrificial samples and have improved image acquisition in 3D space over conventional confocal microscopy. Using SLAM, we propose to predict DDR in non-small cell lung cancer (NSCLC) organoids. NSCLC organoids recapitulate histological and genetic features of their derived patient tissue, retaining tumor variability. Results: Predictive modeling via gradient boost regressor between handcrafted SLAM image features and γH2AX immunostained images on weakly-paired images confirmed strong correlation between SLAM image features and γH2AX speckles/nuclei. Across multiple patient derived NSCLC organoid lines and multiple DNA targeting chemotherapeutics, the optical redox ratio was demonstrated to robustly predict DDR. Conclusion: Label-free imaging via SLAM can be used to predict DDR in live 3D NSCLC organoids with minimal sample handling and without the need to add labelled reporters, enabling quicker and less technically involved DDR assaying. Citation Format: Terrence T. Roh, Aneesh Alex, Janet E. Sorrells, Prasanna Chandramouleeswaran, Marina Marjanovic, Steve R. Hood, BanuPriya Sridharan, Stephen A. Boppart. Label-free multimodal multiphoton microscopy for predicting DNA damage response in patient derived non-small cell lung cancer organoids [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 6613.

MP25-01 AN ETHICAL ROBOTIC SURGICAL TRAINING CURRICULUM USING SIMULATION AND 3D PRINTED SYNTHETIC ORGANS

You have accessJournal of UrologyCME1 Apr 2023MP25-01 AN ETHICAL ROBOTIC SURGICAL TRAINING CURRICULUM USING SIMULATION AND 3D PRINTED SYNTHETIC ORGANS Daniel Costello, Dean Driscoll, Ahmed Ghazi, and Anthony Costello Daniel CostelloDaniel Costello More articles by this author , Dean DriscollDean Driscoll More articles by this author , Ahmed GhaziAhmed Ghazi More articles by this author , and Anthony CostelloAnthony Costello More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000003253.01AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: It is no longer necessary to rely on live animals or cadavers for surgical training. We used 3D printing technology and injection moulding to produce realistic hydrogel synthetic organs for robotic surgery training. These synthetic organ models were a key pillar in a novel multistep robotic surgery curriculum. Phase 1 included an online learning program the first robotic surgery course accredited by the Royal Australasian College of Surgeons. This course included access to a catalogue of live 3D video accessible through VR headsets. Participants then proceeded to VR simulation training, dry lab exercises and human factors training in preparation for procedural training on hydrogel organs. When proficiency was achieved finally live human surgery. Following are results from the synthetic organ robotic procedures. METHODS: Three different synthetic organ models were fabricated for this study; an anatomically correct insufflatable anterior abdominal wall for port placement, a robot assisted partial nephrectomy model and a robot assisted radical prostatectomy model. These models were fabricated using 3D digital models segmented from patient CT images using computer aided-design software. These models were uploaded to 3D printers to generate negative moulds, which were injected with polyvinyl alcohol, a low-cost hydrogel. These positive casts went through freeze thaw cycles achieving different tissue consistencies. These casts were layered in anatomically correct positions creating multi-step procedural models with clinically relevant objective surgical performance metrics. Metrics include blood loss, nerve injury measured by digital strain gauges, cancer margins using fluorescence, anastomosis leak tests, operative time and video based skill assessment. RESULTS: Twenty surgeons performed robotic surgery on 5 synthetic abdomens, 10 hydrogel partial nephrectomies and 30 radical prostatectomies. Participants ranged in experience from novice to experts. Clinically relevant performance metrics were recorded and GEARS video tool was used to assess proficiency. Preliminary validity assessments demonstrate face, content, construct, concurrent and predictive validity. CONCLUSIONS: We demonstrated feasibility and preliminary educational validity of realistic synthetic human organ models for urological robotic surgery. These models offer a viable alternative to live animal and cadaver surgery training, without associated cost, ethical and accessibility drawbacks. Source of Funding: An equipment grant was provided by Intuitive Surgical for simulation surgeries © 2023 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 209Issue Supplement 4April 2023Page: e342 Advertisement Copyright & Permissions© 2023 by American Urological Association Education and Research, Inc.MetricsAuthor Information Daniel Costello More articles by this author Dean Driscoll More articles by this author Ahmed Ghazi More articles by this author Anthony Costello More articles by this author Expand All Advertisement PDF downloadLoading ...

3D bioprinting of human mesenchymal stem cells-laden hydrogels incorporating MXene for spontaneous osteodifferentiation

Contemporary advances in three-dimensional (3D) bioprinting technologies have enabled the fabrication of tailored live 3D tissue mimetics. Furthermore, the development of advanced bioink materials has been highlighted to accurately reproduce the composition of a native extracellular matrix and mimic the intrinsic properties of laden cells. Recent research has shown that MXene is one of promising nanobiomaterials with osteogenic activity for bone grafts and scaffolds due to its unique atomic structure of three titanium layers between two carbon layers. In this study, the MXene-incorporated gelatin methacryloyl (GelMA) and hyaluronic acid methacryloyl (HAMA) (i.e., GelMA/HAMA-MXene) bioinks were prepared to explore if they have the potential to enable the spontaneous osteodifferentiation of human mesenchymal stem cells (hMSCs) when the hMSCs-laden GelMA/HAMA-MXene bioinks were 3D printed. The physicochemical and rheological characteristics of the GelMA/HAMA-MXene hydrogels were proven to be unprecedentedly favorable supportive matrices suited for the growth and survival of hMSCs. Furthermore, hMSCs were shown to spontaneously differentiate into osteoblasts within GelMA-HAMA/MXene composites to provide favorable microenvironments for osteogenesis. Therefore, our results suggest that the remarkable biofunctional advantages of the MXene-incorporated GelMA/HAMA bioink can be utilized in a wide range of strategies for the development of effective scaffolds in bone tissue regeneration.

Assistive mobile application for real-time 3D spatial audio soundscapes toward improving safe and independent navigation

Assistive technologies are used in a variety of contexts to improve the quality of life for individuals that may have one or more vision impairments. This paper describes a novel assistive technology platform that utilizes real-time 3D spatial audio to aid its users in safe and efficient navigation. This platform leverages modern 3D scanning technology on a mobile device to digitally construct a live 3D map of a user's surroundings as they move about their space. Within the digital 3D scan of the world, spatialized, virtual audio sources (i.e., speakers) provide the navigator with a real-time 3D stereo audio "soundscape." As the user moves about the world, the digital 3D map, and its resultant soundscape, are continuously updated and played back through headphones connected to the navigator's device. This paper details (1) the underlying technical components and how they were integrated to produce the mobile application that generates a dynamic soundscape on a consumer mobile device and (2) a methodology for analyzing the usage of the application. It is the aim of this application to assist individuals with vision impairments to navigate and understand spaces safely, efficiently, and independently.

Non-fitting FLIM-FRET facilitates analysis of protein interactions in live zebrafish embryos.

Molecular interactions are key to all cellular processes, and particularly interesting to investigate in the context of gene regulation. Protein-protein interactions are challenging to examine in vivo as they are dynamic, and require spatially and temporally resolved studies to interrogate them. Foerster Resonance Energy Transfer (FRET) is a highly sensitive imaging method, which can interrogate molecular interactions. FRET can be detected by Fluorescence Lifetime Imaging Microscopy (FLIM-FRET), which is more robust to concentration variations and photobleaching than intensity-based FRET but typically needs long acquisition times to achieve high photon counts. New variants of non-fitting lifetime-based FRET perform well in samples with lower signal and require less intensive instrument calibration and analysis, making these methods ideal for probing protein-protein interactions in more complex live 3D samples. Here we show that a non-fitting FLIM-FRET variant, based on the Average Arrival Time of photons per pixel (AAT- FRET), is a sensitive and simple way to detect and measure protein-protein interactions in live early stage zebrafish embryos.

Virtual 3D garment draping as a service

This research details a collaborative project jointly funded by Numerion Software and a Future Fashion Factory innovation challenge grant. The aim was to develop a pioneering, virtual 3D garment draping, cloud-hosted service that was fit for fashion industry needs and could be leveraged with ease. A focus group, consisting of leading fashion designers, brands, manufacturers, educationalists and fashion students, was sent a link to Numerion’s initial prototype together with a questionnaire. This asked participants how the software could be developed; how valuable the proposed service would be, exactly how would individual designers, brands and educationalists use the service; how they would prefer to interact with it and how would it integrate with their current workflow, etc. The data were used to improve and develop the prototype with the intention to trial this in real time with the focus group. The outcomes identified the challenges facing the fashion industry as they seek to embrace live 3D simulation. This influenced the development work required to address any concerns around functionality and frictionless operability to the software as well as to identify a solid path to commercialization for a 3D garment draping service.

3D Printing of Skin Equivalents with Hair Follicle Structures and Epidermal-Papillary-Dermal Layers Using Gelatin/Hyaluronic Acid Hydrogels.

Recent advances in three-dimensional (3D) bioprinting technologies have enabled the fabrication of sophisticated live 3D tissue analogs. Despite the existing hydrogel-based bioinks, the development of advanced bioink materials that can accurately reproduce the composition of a native extracellular matrix and mimic the intrinsic properties of laden cells remains challenging. In this study, 3D printed skin equivalents incorporating hair follicle structures and epidermal/papillary dermal layers were fabricated using gelatin methacryloyl/hyaluronic acid methacryloyl (GelMA/HAMA) bioink. The composition of collagen and glycosaminoglycan in native skin was recapitulated by adjusting the combination of GelMA and HAMA. The GelMA/HAMA bioink exhibited excellent viscoelastic and physicochemical properties, 3D printability, cytocompatibility, and functionality to maintain hair-inductive potency while facilitating spontaneous hair pore development. The results indicate that GelMA/HAMA hydrogels are promising candidates as bioinks for the 3D printing of skin equivalents. Furthermore, they may serve as useful models for skin tissue engineering and regeneration.

709 Stem cell niche architecture dictates hair progenitor distribution and differentiation

Stem cells and their nearby niche cells generate an organ architecture essential to its function. For hair production, the hair follicle remodels its epithelial architecture through stem cell behaviors under the instruction of a juxtaposed fibroblast niche -- dermal papilla. Although dermal papilla’s signaling crosstalk with hair follicle epithelium is well-characterized, little is known about how dermal papilla’s physical architecture is organized to support epithelial stem cells. Here, we leveraged live mice imaging, 3D reconstruction, and genetic manipulations to understand how dermal papilla fibroblasts remodel its niche architecture in orchestration with the stem cell progeny -- hair progenitors.

Software Tool for Automatic Quantification of Sarcomere Length and Organization in Fixed and Live 2D and 3D Muscle Cell Cultures In Vitro.

Sarcomeres are the structural units of the contractile apparatus in cardiac and skeletal muscle cells. Changes in sarcomere characteristics are indicative of changes in the sarcomeric proteins and function during development and disease. Assessment of sarcomere length, alignment, and organization provides insight into disease and drug responses in striated muscle cells and models, ranging from cardiomyocytes and skeletal muscle cells derived from human pluripotent stem cells to adult muscle cells isolated from animals or humans. However, quantification of sarcomere length is typically time consuming and prone to user-specific selection bias. Automated analysis pipelines exist but these often require either specialized software or programming experience. In addition, these pipelines are often designed for only one type of cell model in vitro. Here, we present an easy-to-implement protocol and software tool for automated sarcomere length and organization quantification in a variety of striated muscle in vitro models: Two dimensional (2D) cardiomyocytes, three dimensional (3D) cardiac microtissues, isolated adult cardiomyocytes, and 3D tissue engineered skeletal muscles. Based on an existing mathematical algorithm, this image analysis software (SotaTool) automatically detects the direction in which the sarcomere organization is highest over the entire image and outputs the length and organization of sarcomeres. We also analyzed videos of live cells during contraction, thereby allowing measurement of contraction parameters like fractional shortening, contraction time, relaxation time, and beating frequency. In this protocol, we give a step-by-step guide on how to prepare, image, and automatically quantify sarcomere and contraction characteristics in different types of in vitro models and we provide basic validation and discussion of the limitations of the software tool. © 2022 The Authors. Current Protocols published by Wiley Periodicals LLC. Basic Protocol: Staining and analyzing static hiPSC-CMs with SotaTool Alternate Protocol: Sample preparation, acquisition, and quantification of fractional shortening in live reporter hiPSC lines Support Protocol 1: Finding the image resolution Support Protocol 2: Advanced analysis settings Support Protocol 3: Finding sarcomere length in non-aligned cells.

Automated high-speed 3D imaging of organoid cultures with multi-scale phenotypic quantification.

Current imaging approaches limit the ability to perform multi-scale characterization of three-dimensional (3D) organotypic cultures (organoids) in large numbers. Here, we present an automated multi-scale 3D imaging platform synergizing high-density organoid cultures with rapid and live 3D single-objective light-sheet imaging. It is composed of disposable microfabricated organoid culture chips, termed JeWells, with embedded optical components and a laser beam-steering unit coupled to a commercial inverted microscope. It permits streamlining organoid culture and high-content 3D imaging on a single user-friendly instrument with minimal manipulations and a throughput of 300 organoids per hour. We demonstrate that the large number of 3D stacks that can be collected via our platform allows training deep learning-based algorithms to quantify morphogenetic organizations of organoids at multi-scales, ranging from the subcellular scale to the whole organoid level. We validated the versatility and robustness of our approach on intestine, hepatic, neuroectoderm organoids and oncospheres.

63‐4: A Novel Real‐Time Full‐Color 3D Holographic (Diffractive) Video Capture, Processing, and Transmission Pipeline Using Off‐the‐Shelf Hardware

This paper details the world's first live 3D holographic (diffractive) video call using off‐the‐shelf hardware. We introduce a novel pipeline that facilitates the capture, processing, and transmission of RGBZ data, using an iPhone for image and depth capture with VividQ's SDK for hologram generation and hardware for display.

Quantifying cell-cycle-dependent chromatin dynamics during interphase by live 3D tracking

SummaryThe study of cell cycle progression and regulation is important to our understanding of fundamental biophysics, aging, and disease mechanisms. Local chromatin movements are generally considered to be constrained and relatively consistent during all interphase stages, although recent advances in our understanding of genome organization challenge this claim. Here, we use high spatiotemporal resolution, 4D (x, y, z and time) localization microscopy by point-spread-function (PSF) engineering and deep learning-based image analysis, for live imaging of mouse embryonic fibroblast (MEF 3T3) and MEF 3T3 double Lamin A Knockout (LmnaKO) cell lines, to characterize telomere diffusion during the interphase. We detected varying constraint levels imposed on chromatin, which are prominently decreased during G0/G1. Our 4D measurements of telomere diffusion offer an effective method to investigate chromatin dynamics and reveal cell-cycle-dependent motion constraints, which may be caused by various cellular processes.

Advances in Procedural Echocardiographic Imaging in Transcatheter Edge-to-Edge Repair for Mitral Regurgitation.

Transcatheter edge-to-edge repair (TEER) therapy is recommended by the American College of Cardiology/American Heart Association (ACC/AHA) guidelines for selected patients with symptomatic severe or moderate-severe mitral regurgitation (MR). Echocardiography, in particular transesophageal echocardiography (TEE), plays a critical role in procedural planning and guidance for TEER. Recent innovations and advances in TEE techniques including three-dimensional (3D) imaging, unlimited x-plane imaging, live 3D multiplanar reconstruction, as well as transillumination imaging with color Doppler and transparency rendering have further enhanced procedural imaging for TEER, especially for complex diseases including commissural defects, clefts, and multi-segment pathologies. This review discusses the technology of these advanced procedural imaging techniques and provides a “step-by-step” guide on how to apply them during the TEER procedure with a focus on their added values in treatment of complex valve lesions.

High-fidelity 3D real-time facial animation using infrared structured light sensing system

The acquisition of 3D facial models is crucial in the gaming and film industries. In this study, we developed a facial acquisition system based on infrared structured light sensors to obtain facial-expression models with high fidelity and accuracy. First, we employed time-multiplexing structured light to obtain an accurate and dense point cloud. The template model was then warped to the captured facial expression. A model-tracking method based on optical flow was applied to track the registered 3D model displacement. Finally, the live 3D mesh was textured using high-resolution images captured by three color cameras. Experiments were conducted on human faces to demonstrate the performance of the proposed system and methods.

Live 3D imaging and mapping of shear stresses within tissues using incompressible elastic beads.

To investigate the role of mechanical constraints in morphogenesis and development, we have developed a pipeline of techniques based on incompressible elastic sensors. These techniques combine the advantages of incompressible liquid droplets, which have been used as precise in situ shear stress sensors, and of elastic compressible beads, which are easier to tune and to use. Droplets of a polydimethylsiloxane mix, made fluorescent through specific covalent binding to a rhodamin dye, are produced by a microfluidics device. The elastomer rigidity after polymerization is adjusted to the tissue rigidity. Its mechanical properties are carefully calibrated in situ, for a sensor embedded in a cell aggregate submitted to uniaxial compression. The local shear stress tensor is retrieved from the sensor shape, accurately reconstructed through an active contour method. In vitro, within cell aggregates, and in vivo, in the prechordal plate of the zebrafish embryo during gastrulation, our pipeline of techniques demonstrates its efficiency to directly measure the three dimensional shear stress repartition within a tissue.

A no-Reference Stereoscopic Image Quality Assessment Network Based on Binocular Interaction and Fusion Mechanisms.

In contemporary society full of stereoscopic images, how to assess visual quality of 3D images has attracted an increasing attention in field of Stereoscopic Image Quality Assessment (SIQA). Compared with 2D-IQA, SIQA is more challenging because some complicated features of Human Visual System (HVS), such as binocular interaction and binocular fusion, must be considered. In this paper, considering both binocular interaction and fusion mechanisms of the HVS, a hierarchical no-reference stereoscopic image quality assessment network (StereoIF-Net) is proposed to simulate the whole quality perception of 3D visual signals in human cortex, including two key modules: BIM and BFM. In particular, Binocular Interaction Modules (BIMs) are constructed to simulate binocular interaction in V2-V5 visual cortex regions, in which a novel cross convolution is designed to explore the interaction details in each region. In the BIMs, different output channel numbers are designed to imitate various receptive fields in V2-V5. Furthermore, a Binocular Fusion Module (BFM) with automatic learned weights is proposed to model binocular fusion of the HVS in higher cortex layers. The verification experiments are conducted on the LIVE 3D, IVC and Waterloo-IVC SIQA databases and three indices including PLCC, SROCC and RMSE are employed to evaluate the assessment consistency between StereoIF-Net and the HVS. The proposed StereoIF-Net achieves almost the best results compared with advanced SIQA methods. Specifically, the metric values on LIVE 3D, IVC and WIVC-I are the best, and are the second-best on the WIVC-II.