This study aimed to test whether three different cement layer thicknesses (60, 120 and 180 μm) would provide the same bonding capacity between adhesively luted lithium disilicate and human dentin. Ceramic blocks were cut with a low-speed diamond saw with water cooling into 20 blocks, which were cemented to human flat dentin with adhesive protocol. The assembly was sectioned into 1 mm 2 cross-section beams composed of ceramic/cement/dentin. Cement layer thickness was measured, and three groups were formed. Half of the samples were submitted to aging simulation and the other half was immediately tested to evaluate the long-term bond strength. The microtensile test was performed in a universal testing machine. Bond strength (MPa) was calculated. The fractured specimens were examined under stereomicroscopy. Applying the finite element method, the residual stress of polymerization shrinkage according to cement layer thick-ness was also calculated using First Principal Stress as analysis criteria. ANOVA showed that the cement layer thickness factor significantly influenced the bond strength results for the aged samples (p=0.040) without difference between the groups for the immediately tested groups (p=0.772). The higher the cement layer thickness the higher the residual stress generated at the adhesive interface due to cement polymerization shrinkage. In conclusion, the cement layer thickness does not affect the immediate bond strength in lithium disilicate restorations; however, thinner cement layers are most stable in long-term showing constant bond strength and lower residual stress.