Hitoshi Sohma1,2, Mami Yamaguchi1, Michitoshi Kimura1, Shin-ichi Imai1, Kayo Matsumoto1, Norio Takei1 and Yasuo Kokai1 1Department of Biomedical Engineering, Sapporo Medical University School of Medicine, Japan 2Department of Educational Development, Sapporo Medical University Center for Medical Education, Japan Objective: Alzheimer’s disease (AD) diff ers from other forms of dementia in its relation to amyloid beta peptide (Abeta42). Abeta42, a proteolytic product of amyloid precursor proteins (APP), has a toxic eff ect on neuronal cells. Th is eff ect implies that protein expression is changed in neuronal cells by Abeta42, which provides a molecular marker for this disease. In the present study, we used the mice primary culture neurons and investigated the proteins in the supernatant aft er incubation with or without Abeta42. Methods: In view of the appearance of an acidic phospholipid (phosphatidylserine (PS)) on the outer plasma membrane of an apoptotic cell, we used PS as a probe and proteins bound to PS-coated magnetic nano-beads in a Ca2+-dependent manner were identifi ed using a proteomic approach.