Linyi City Research Articles

Analysis of the Coordination of Built-Up Area Expansion and Population Growth in the Shandong Peninsula Urban Agglomeration

Exploring the coordinated relationship between built-up area expansion and population growth in the urbanization process is important for the planning and sustainable development of cities. The article took Shandong Peninsula urban agglomeration as the study area, which was divided into the Provincial Capital Economic Circle, Jiaodong Economic Circle, and Lunan Economic Circle according to the plan named the Implementation Plan for Implementing the Opinions of the CPC Central Committee and the State Council on Establishing a New Mechanism for More Effective Regional Co-ordinated Development. The people–land coordination relationship in the region was analyzed from the perspective of spatio-temporal integration through indices such as fractal dimension, spatial autocorrelation, population sprawl, and city land expansion and population growth coordination index (CPI) The results showed that (1) the relationship between built-up area expansion and population growth in the Shandong Peninsula urban agglomeration was uncoordinated. The three economic circles mainly changed from rapid population growth to significant expansion of built-up areas. (2) The development patterns of the three economic circles were different. The Provincial Capital and Jiaodong showed a pattern with Jinan and Qingdao as the core, driving the periphery area. Lunan showed a multi-point scattered development; there was no central city, but Linyi City was developing faster. (3) Jiaodong had the biggest changes in terms of population and built-up area. This study provides a scientific reference for the urban planning and sustainable development of the Shandong Peninsula urban agglomeration.

Fungal aerosols in rabbit breeding environment: Metagenetic insight into PM2.5 based on third-generation sequencing technology

Fungal aerosols are a vital environmental hazard factor impeding the development of the rabbit breeding industry and threatening public health. This study aimed to determine fungal abundance, diversity, composition, diffusion, and variability in aerosols in rabbit breeding environments. Twenty PM2.5 filter samples were collected from five sampling sites (i.e. En5, In, Ex5, Ex15, and Ex45) in a modern rabbit farm in Linyi City, China. Fungal component diversity at the species level was analyzed in all samples using third-generation sequencing technology. Results revealed that fungal diversity and community composition in PM2.5 significantly differed across different sampling sites, and different pollution levels. The highest concentrations of PM2.5 and fungal aerosols (i.e., 102.5 μg/m3 and 18.8 × 103 CFU/m3, respectively) were found at Ex5, and these concentrations were found to decrease as the distance from the exit increased. However, no significant correlation was observed between the internal transcribed spacer (ITS) gene abundance and overall PM2.5 levels, except for Aspergillus ruber and Alternaria eichhorniae. Although most fungi are not pathogenic to humans, zoonotic pathogenic microorganisms that cause pulmonary aspergillosis (e.g., Aspergillus ruber) and invasive fusariosis (e.g., Fusarium pseudensiforme) were observed. The relative abundance of A. ruber was higher at Ex5 than that at In, Ex15, and Ex45 (p < 0.01), and the relative abundance of the fungal species decreased with an increase in distance from the rabbit houses. Moreover, four potential novel strains of Aspergillus ruber were discovered, with 82.9%–90.3% of the nucleotide and amino acid sequences similar to those of reference strains. This study highlights the importance of rabbit environments as a source in shaping fungal aerosol microbial communities. To the best of our knowledge, this is the first study to uncover the initial characteristics of fungal biodiversity and dispersion of PM2.5 in rabbit breeding environments, contributing to infectious disease control and prevention in rabbits.

Volatile Organic Compounds in the North China Plain: Characteristics, Sources, and Effects on Ozone Formation

Enhanced volatile organic compounds (VOCs) observations were made on ozone-exceeding days in June 2020 in Linyi, China. A total of 69 VOCs were collected (1 alkyne, 29 alkanes, 10 alkenes, 14 aromatic hydrocarbons, and 15 oxygenated volatile organic compounds (OVOCs)). The average concentration of total VOCs (TVOCs) was 36.0 ± 0.66 ppb, and the top three VOCs components were alkanes, OVOCs, and aromatic hydrocarbons, which accounted for 40.75%, 27.02%, and 11.30%, respectively. Based on the positive matrix factorization (PMF) model, the main sources of VOCs in Linyi City were divided into vehicle exhaust sources (39.11%), biomass combustion sources (21.82%), oil and gas volatilization sources (21.46%), and solvent use sources (17.61%). The ozone formation potential (OFP) contribution rate was dominated by alkenes, OVOCs, and aromatics, with contribution rates of 26.37%, 25.30%, and 23.65%, respectively. The top six VOCs that contributed the most to the OFP were formaldehyde, acetaldehyde, 1-butene, butadiene, trans-2-butene, and propylene. The empirical kinetic modelling approach (EKMA) curve indicated that the in situ ozone (O3) production was limited by VOCs, and reducing the concentration of O3 precursors in accordance with the VOCs/NOx concentration ratio of 1.15 can control O3 pollution more effectively.

Swot Analysis and Feasibility of Museum Cluster Development in Linyi City, China

Based on the realistic background of the development of museums in Linyi City, Shandong Province, this paper makes a SWOT analysis of the development of the museum cluster in Linyi City. The development advantages of the museum cluster in Linyi City mainly include superior geographical location, the emergence of a red tourism brand, the promotion of resource sharing and complementation, and the realization of collaborative promotion and marketing. However, the development of the museum cluster may inevitably involve unfavourable factors and disadvantages in popularity and influence, construction and operation costs, competition and cooperation, and interest distribution. Even so, developing museum clusters in Linyi City is beneficial to realize opportunities such as resource sharing and complementarity, collaborative promotion and marketing, forming cultural tourism destinations, and promoting academic exchanges and cooperation. Meanwhile, it is necessary to coordinate the cooperative relationship among multiple museums, which may lead to challenges in the distribution of benefits, cultural differences and ideas, resource sharing and mutual assistance, and organization and management in promoting the museum clusters in Linyi City. In future development, Linyi City can improve the visibility and influence of the city through the development of museum clusters, attract more tourists and funds, and promote the innovation and development of cultural and tourism undertakings. The government should also increase its support for cultural undertakings and provide more policy support for developing museum clusters.

Exploring the Management of School Security: A Case Study in a China High School

This study is a case study that aims to explore the current situation, issues, and strategies faced by leaders and managers of a regular high school in Linyi City, Shandong Province, China, about security management. This study uses qualitative method, with semi-structured interviews conducted with three faculty members and managers of a high school in Linyi City. This study reveals that safety management in this school focuses on safety education and has set up monitoring systems, alarm systems, etc., and conduct safety drills from time to time. And regular feedback is given through summary meetings and other forms. There are still issues such as inadequate staffing, lack of cooperation between teachers and students, and lack of home-school contact and monitoring systems. As a result, various measures such as increasing security guards, setting up online monitoring platforms, and enhancing security knowledge education have been proposed. The findings of this study have implications for enhancing the level of security management in schools. In future studies, the sample should be expanded and the level of security management in elementary schools and universities should also be assessed.

Research on the Management of Street Stall Economy during the Regular Epidemic Prevention and Control

This study uses literature research method, comparative research method and qualitative analysis method, from the perspective of economics, with the background of Linyi City's normalized epidemic prevention and control period, to collect and analyze the urban areas of Linyi City before and after the normalized epidemic prevention and control. The relevant data on the economic management of street stalls sorted out three problems in the economic management of street stalls during the period of normalized epidemic prevention and control: high management costs and low returns; low level of third-party management; and lack of support from the masses. Conclusion is to improve government management, standard third-party institutions, and strengthen publicity and guidance to provide countermeasures and suggestions to improve the effectiveness of the economic management of street stalls in Linyi City during the period of normalized epidemic prevention and control. Urban management under other public health crisis conditions has certain reference value.

Research on Energy Saving and Economy of Old Buildings Based on Parametric Design: A Case Study of a Hospital in Linyi City, Shandong Province

With the need for a green economy and the introduction of low-carbon policies, more and more old buildings are being transformed to improve the thermal performance of buildings and reduce energy consumption. However, people ignore the village buildings and the old public buildings. Based on the investigation of a typical health center in Linyi City, this paper proposed improvement measures using EnergyPlus simulation software and JePlus parameter chemical tools. The various factors analyze the various parts of the enclosure, and the energy-saving effect of the roof is better than that of the exterior wall: When the thickness of the insulation layer gradually increases from 0 cm to 30 cm, the unit energy consumption of the building gradually decreases from 57 kW·h/m2 to 42.8 kW·h/m2, while that of the roof is gradually reduced to 24.41 kW·h/m2. Double exterior windows effectively reduce the energy consumption of the building in all renovation schemes. The optimal scheme is selected through the parameterized design of all the overall enclosure reconstruction schemes and the initial investments. The results showed that the investment recovery of the selected scheme was between 1.36–5.28 years. Plan 5 (12 cm XPS is used as insulation material for the exterior wall and roof, and the exterior window is replaced with 6 mm medium transmittance Low-e + 12 mm air + 6 mm clear) had the highest energy efficiency (91.11%). Plan 2 (6 cm XPS is used as insulation material for the exterior wall and roof, and the exterior window is replaced with 12 mm single medium transmittance heat-reflecting glass) had the shortest investment recovery period (2.04 years).

Towards Improving Rural Living Environment for Chinese Cold Region Based on Investigation of Thermal Environment and Space Usage Status

In recent years, many scholars worldwide have carried out research related to the relationship between the construction of rural dwellings and the indoor thermal environment in response to the issue of building sustainability. China’s rural revitalization has developed rapidly in recent years. Although previous research showed that the poor indoor thermal environment of vernacular dwellings has negative effects on residents’ thermal comfort and health, it is poorly studied in terms of residents’ space usage habits, their thermal comfort satisfaction, and their thermal sensation. Linyi, the largest and most populous city in Shandong, China, was selected as the research area to investigate the thermal environment and space usage by using in-depth questionnaire interviews and systematical measurements. The results show that there are seasonal differences in space usage in a typical vernacular dwelling. The field measurements show that the indoor temperature is 0–5 °C in winter if there is no heating and 26 °C in summer. By comprehensive comparison, it is found that there is a contradiction between residential space usage and the functional layout; residents choose to use rooms with worse thermal conditions in winter. The analysis of the questionnaires shows that residents have higher satisfaction with the indoor thermal environment in summer than in winter, and the thermal comfort range of residents is 23.3~30 °C in summer and 11.25~17.5 °C in winter. Additionally, the neutral thermal temperature of the indoor space is 26.7 °C in summer and 14.4 °C in winter. Although residents have better adaptability to the cold climate, the measurement data in winter are still beyond the comfort range. We suggest that these results can be used as a reference and data base for improving the living environment of rural vernacular dwellings in Chinese cold regions.

Rickettsia sp. and Anaplasma spp. in Haemaphysalis longicornis from Shandong province of China, with evidence of a novel species “Candidatus Anaplasma Shandongensis”

Haemaphysalis longicornis is one of the most dominant and widespread tick species in China. This species mainly infests wild animals and occasionally attacks humans, and has been associated with the transmission of a variety of zoonotic pathogens including spotted fever group Rickettsia (SFGR), severe fever with thrombocytopenia syndrome virus (SFTSV), Anaplasma phagocytophilum, Babesia spp. and Theileria spp.. Although there are increasing reports of various pathogens associated with H. longicornis, some neglected pathogens in certain areas still need to be studied. In this study, a total of 171 H. longicornis ticks were collected from goats in three locations of Shandong Province, Eastern China (Zibo, Linyi, and Qingdao cities), and subsequently screened for the presence of Rickettsia, Anaplasma, and Ehrlichia bacteria. A total of four bacterial species were identified and characterized. “Candidatus Rickettsia jingxinensis” was detected in one tick specimen from Zibo city. Of 98 ticks from Linyi city, 63.27% (62/98) were tested positive for Anaplasma capra and 5.10% (5/98) were positive for Anaplasma bovis. Interestingly, a novel Anaplasma species was detected and characterized in one tick specimen from Zibo and one other from Linyi, respectively. Genetic and phylogenetic analysis based on the 16S, gltA, groEL, and msp4 genes indicated that it was divergent from all known Anaplasma species but mostly related to A. phagocytophilum and “Cadidatus Anaplasma boleense”. Based on where it was first detected, we named it “Candidatus Anaplasma shandongensis”.

Automatic Pavement Crack Detection Fusing Attention Mechanism

Pavement cracks can result in the degradation of pavement performance. Due to the lack of timely inspection and reparation for the pavement cracks, with the development of cracks, the safety and service life of the pavement can be decreased. To curb the development of pavement cracks, detecting these cracks accurately plays an important role. In this paper, an automatic pavement crack detection method is proposed. For achieving real-time inspection, the YOLOV5 was selected as the base model. Due to the small size of the pavement cracks, the accuracy of most of the pavement crack deep learning-based methods cannot reach a high degree. To further improve the accuracy of those kind of methods, attention modules were employed. Based on the self-building datasets collected in Linyi city, the performance among various crack detection models was evaluated. The results showed that adding attention modules can effectively enhance the ability of crack detection. The precision of YOLOV5-CoordAtt reaches 95.27%. It was higher than other conventional and deep learning methods. According to the pictures of the results, the proposed methods can detect accurately under various situations.

Relationship between Soil Organic Carbon, Soil Nutrients, and Land Use in Linyi City (East China)

The distribution characteristics of soil organic carbon (SOC) and soil nutrients under different land-use types in Linyi City, East China, were studied. The spatial distribution of SOC under different land-use types and the relationship between SOC and soil nutrients were analyzed using remote sensing interpretation and soil sample analyses. The results showed that SOC in Linyi was mainly stored in drylands and paddy fields. SOC and total nitrogen (TN) levels were positively correlated for most land use types. There was a positive linear correlation between SOC and total K in the paddy fields. The coefficients of variation for SOC and TN differed greatly among the land use types studied. Total SOC storage was 8772.73 × 103 kg in the surface 0.2 m soil layer. The order of total SOC storage was drylands (6771.45 × 103 ton) &gt; paddy field (764.67 × 103 ton) &gt; nurseries (510.79 × 103 ton) &gt; forest land (413.49 × 103 ton) &gt; low-cover grasslands (238 × 103 ton) &gt; bare land (74.35 × 103 ton). With the largest SOC storage, and C density, respectively, drylands and paddy fields are conducive to enhancing soil C sequestration, achieving low-carbon goals, and mitigating climate change.

Genetic evolution of fowl adenovirus serotype 4 and its pathogenicity to Cherry Valley ducks in China

Hydropericardium hepatitis syndrome (HHS), a novel poultry disease, is caused by fowl adenovirus 4 (FAdV-4). It mainly infects 3–5-week-old broilers. In July 2015, the first outbreak of HHS occurred in the broilers in east China, which caused great economic losses to the poultry industry. In June 2019, infectious disease was detected with suspected HHS symptoms on a duck farm in Linyi City, Shandong Province. The main necropsy lesions included pericardial effusion and hepatitis. In this study, we isolated a strain of FAdV-4 from naturally infected ducks and named it SDLY190604, and the hexon gene sequence was amplified and analyzed using polymerase chain reaction (PCR). In order to study the effect of FAdV-4 on Cherry Valley ducks, we inoculated three-week-old ducks with 0.2 ml of FAdV-4 virus fluid (TCID50 of 10-6.3/0.1 ml) by orally, subcutaneously and intramuscularly. Clinical signs, gross lesions and histopathological changes, cytokines and viral load were detected and recorded within 15 days after infection. The results showed that ducks in the experimental groups exhibited typical symptoms of hydropericardium and hepatitis. The histopathological sections showed multiple-organ damage, including serious liver and kidney damage with elevated levels of inflammatory cytokines, probably due to the infection and innate immune response. Later, immunosuppression occurred, resulting in decreased levels of cytokines. The viral load indicated that the virus could be present in several organs of the ducks, with the highest viral DNA found in the liver, followed by the kidney. Compared to the subcutaneous and oral groups, the intramuscular group exhibited the highest viral load. In summary, this study can increase our understanding of the pathogenicity of FAdV-4 in ducks and provide a basis for further understanding of the virus, imparting new insights into disease research.

A Study on the Different Values Assigned to Bulls and Cows under the Structure of Male and Female Workpoints in Collectivist China: The Practice of Lingqian Village in Shandong Province

Abstract During the collectivization period, the price of the bull was higher than the price of the cow on the fixed asset registration form in Lingqian Village, Linyi City, Shandong Province. The workpoints of male labor were also higher than those for female labor. Therefore, there was a gender difference that applied to both cattle and humans, but the former was not simply the social projection and cultural metaphor of the latter; rather they shared the same natural difference in terms of economic value in that given system. Namely, the values of humans and cattle depended entirely on their contribution to agricultural labor, not on gender discrimination. The reason for this phenomenon is that the ecological environment and living conditions shaped people’s psychology, which then determined the internal classification structure of gender in humans as well as animals. The classification attitude of the values of humans and animals was thus a result of adaptation to the natural environment and living conditions, and, as such, was a collective survival strategy, but it was also the product of a particular economic system. This phenomenon resulted from the fact that the privately owned land of individual families had been collectivized, revealing some characteristics of the cattle-raising mode under the collective economic system. The case of Lingqian Village in Shandong Province contributes to world anthropology by illustrating one particular system of social livelihood, with cattle as the reference point.

Research on the Current Characteristics and Countermeasures of Population Aging—A Case Study of Linyi City

Research on the Current Characteristics and Countermeasures of Population Aging—A Case Study of Linyi City

Source Identification and Health Risk Assessment of Heavy Metals in Soil: A Case Study of Lintancang Plain, Northeast China.

To investigate the concentration, source, and potential health risk of soil heavy metals (V, Cr, Ni, Cu, Zn, Pb, Hg), this study determined the concentration of these seven metals in 37 soil samples from Linyi City, southeast of Shandong Province, China. The mean concentrations of the investigated heavy metals followed the sequence: Cr (76.2 mg/kg) > V (70.5 mg/kg) > Zn (70.1 mg/kg) > Ni (34.0 mg/kg) > Pb (31.4 mg/kg) > Cu (23.2 mg/kg) > Hg (1.7 mg/kg). The enrichment factor (EF) and geo-accumulation index (Igeo) indicated an extreme enrichment of Hg (EF > 10, Igeo > 4) within the study area, while a slight enrichment of other metals. According to the toxic risk index (TRI), Hg accounted for the strongest soil toxicity (TRI = 8.07, 64.3%). The risk assessment with hazard index (HI) suggested that the health risks of all metals were acceptable, and the HI of adults was generally lower compared with that of the children. In addition, two principal components (PC) calculated by principal component analysis (PCA) were used to identify the sources of these heavy metals, which were 57.73% for PC 1 (Pb, Cr, Zn, Ni, Hg, Cu and V) and 21.63% for PC 2 (Hg, Cu and V), respectively. Moreover, PC 1 was mainly controlled by anthropogenic inputs, while PC 2 was contributed to by natural sources. Combined with the correlation matrix, it was concluded that there were three different sources for all seven heavy metals.

First Report of Postharvest Fruit Rot Disease of Yellow Peach Caused by Diaporthe eres in China.

The yellow peach (Amygdalus persica), is a fruit crop native to China with golden peel and pulp that is of particular interest in the fruit markets. In August of 2021, yellow peaches showing fruit rot symptoms were purchased from a commercial market in Linyi city, Shandong province, China. The symptoms included circular, tan to brown in color, rotten, necrotic lesions, and whitish mycelium mass in the center of the lesions. The infected fruit were surface disinfected with 1% NaClO for 30 s and rinsed with sterile distilled water three times. Diseased tissues from the infected fruits were cut into small segments, aseptically shifted onto potato dextrose agar media containing petri plates and incubated at 25℃ for 5 days. Eight isolates were obtained in total from two isolation experiments. Fungal colonies were initially white, aerial, fluffy at first, and gradually turned brown to gray, with black stromata at maturity. Alpha conidia were aseptate，hyaline，fusiform to ellipsoidal，and ranged in size from 4.16 to 7.76 µm × 1.95 to 3.14 µm (n=30). Beta conidia were aseptate, hyaline, filiform, curved to hamate, and 15.91 to 22.55 µm × 0.82 to 1.66 µm (n=30). The morphological characteristics were consistent with those of Diaporthe species (Gomes et al. 2013). For further identification, a multigene phylogenetic analysis was carried out. The internal transcribed spacer (ITS) region, translation elongation factor 1-α (TEF1-α), histone H3 (HIS), calmodulin (CAL), and β-tubulin (TUB) genes of two representative isolates were amplified by using primers ITS1/ITS4, EF1-728F/EF1-986R, CYLH3F/H3-1b，CAL228F/CAL737R, and Bt2a/Bt2b (Chaisiri et al. 2021), respectively. The sequences were deposited in GenBank (Accession No. OL375154 for ITS; OL406409 for TEF1-α; OL406410 for HIS; OL106407 for CAL; OL406408 for TUB). phylogenetic analyses were conducted using the concatenation of multiple sequences (ITS, TEF1-α, HIS, CAL, TUB) with Maximum Likelihood (ML) in IQtree v1.5.6 (Nguyen et al. 2015). Based on the morphological and phylogenetic characters, the isolates were identified as D. eres. A Pathogenicity test was performed by wound inoculation on harvested fruits of A. persica Variety 'Jinxiu'. Mature and healthy yellow peaches purchased from Shandong, Anhui, and Hunan Provinces in China were surface sterilized with 1% NaClO solution for 1 minute, rinsed with sterile water and dried. Each fruit was wounded with a sterile scalpel creating a 2-3 mm incision on the peel. A 5 mm agar disc with mycelium grown on PDA at 28℃ for 7 days was placed on wound and sealed with parafilm. Sterile PDA plugs were used as controls. Ten fruit were used for each treatment and the assays were repeated three times. Inoculated fruit were placed in sterilized transparent plastic cans containing wet, sterile paper towels. After 5 days of incubation at 25℃, the same rot symptoms were observed on fruits inoculated with mycelium and the control remained symptomless. D. eres was re-isolated from the lesions of inoculated fruits and the pathogen identification was confirmed by molecular analysis, thus fulfilling complete Koch's postulates. Although D. eres was previously reported on peach trees of causing shoot blight (Thomidis and Michailides 2009) and stem canker (Prencipe et al. 2017). To our knowledge, this is the first report of D. eres causing postharvest fruit rot of yellow peach in China and it may lead to considerable economic losses in the peach industry should post-harvest disease management practices not be implemented.

Simulation of adult-child matching behavior: A case study of primary school in Linyi city, China

Pedestrian evacuation at the gate of primary school after school is always a tricky question bothering us. In order to improve the safety and efficiency of pedestrian evacuation at the gate of primary school, adult–child matching behavior after school is analyzed. We propose four adult–child matching strategies considering order or random of adults and children in the matching process. Fusing the matching strategy into a cellular automata (CA)-based pedestrian simulation model and taking the Linyi No. 40 middle school, China as the case study, the adult–child matching behavior is simulated on the platform of MATLAB software. The simulation results indicate that evacuation efficiency of the strategy (adults are orderly distributed in the first waiting area and children are orderly queuing up) reaches the maximum value among the four strategies. Effective matching strategies could reduce pedestrian conflicts. The randomly children and randomly adults are all the negative impacts for evacuation. Orderly evacuation process could reduce pedestrian conflicts. Compared with the randomly children, the effect on evacuation efficiency of the randomly adults is larger. A reasonable number of evacuated pedestrians at a time could improve the whole evacuation efficiency, and we advise the Linyi No. 40 middle school to evacuate three classes at a time.

First Report of New Bacterial Leaf Streak of Rice Caused by Pantoea ananatis in China.

Rice (Oryza sativa L.) is the largest grain crop, accounting for about 40 % of the total grain production in China. In mid-July 2021, bacterial leaf streak-like disease emerged in rice varieties Chunyou584 and Yongyou2604 in Linyi city, Shandong Province, China. Disease incidences of the disease ranged from 80% to 90% in the surveyed fields. Infected rice leaves displayed dark green to yellowish-brown water-soaked thin streaks, and a large amount of beaded yellow oozes were observed on the lesions. After drying, there were gelatinous granules that were not easy to fall off and spread between leaf veins (Fig.S1A). According to the field symptoms of this disease, it was preliminarily suspected to be rice bacterial leaf streak caused by Xanthomonas oryzae pv. oryzicola (Xoc), which is a guaranteed disease in China. To isolate the causal agent, leaf discs (~1 cm2) of diseased leaves were collected from the margins of the lesions, surface sterilized and ground into pieces in sterile double distilled water. The 10-3, 10-4 and 10-5 dilutions were spread onto peptone sugar agar (PSA) and incubated at 28°C for 36 hours. Yellow mucous bacterial colonies were consistently obtained on PSA medium. To identify the pathogen, fragments of the 16S rDNA, leuS and rpoB were amplified and sequenced using the primers previously reported (Yu et al. 2021). Three strains (LY01, LY02 and LY03) showed identical colony morphology and LY01 was used for further analyses. Sequence analyses showed that the fragments of 16S rDNA (955 bp, GenBank accession number: OK261898), leuS (755 bp, GenBank accession number: OK298387) and rpoB (926 bp, GenBank accession number: OK298388) of strain LY01 shared 99.16%, 99.46% and 100% similarities with those of Pantoea ananatis TZ39 (GenBank accession numbers: CP081342.1 for 16S rDNA, MW981338.1 for leuS and MW981344.1 for rpoB), respectively, which suggest the pathogenic bacterial strain LY01 isolated is P. ananatis. In addition, the single colony of P. ananatis LY01 was shown as Fig. S2B. Furthermore, pathogenicity tests were also performed according to the following steps. Bacterial suspension at OD600=0.1 was inoculated into eight rice leaves of four healthy rice plants (Chunyou 584) at 25-33°C and 60%-80% relative humidity in the field using a clipping method (Yang et al. 2020) or spraying methods, and sterile distilled water was as negative control. The clipped leaves (Fig. S1B) and spray-inoculated leaves (Fig. S1C) showed dark green water-soaked streaks at 14 days after inoculation, respectively, which showed similar symptoms with those samples collected from the fields (Fig. S1A). On contrary, the control rice leaves remained healthy and symptomless (Fig. S2A). The bacterium was re-isolated in the inoculated rice leaves and the re-isolated bacterial isolates, which was confirmed by sequencing 16S rDNA, leuS and rpoB, incited the same symptoms as in fields, which fulfills Koch's postulates. In the past decade, P. ananatis was reported to result in grain discoloration and leaf blight in China (Yan et al. 2010; Xue et al. 2020, Yu et al. 2021), which could result in 40% - 60% yield losses. To our best knowledge, this is the first report of the bacterial leaf streak-likely disease occurred in Shandong Province caused by P. ananatis, so we named it as Pantoea leaf streak of rice. Although P. ananatis was also reported in Zhejiang province and Jiangxi province, which caused leaf streak lesions on rice, the disease symptoms are completely different from those of Pantoea leaf streak of rice. To the best of our knowledge, this is the first report of Pantoea leaf streak of rice caused by P. ananatis. This study provides sloid evidence that Pantoea leaf streak of rice in Eastern China can be caused by the new pathogen, P. ananatis, rather than Xoc as traditionally assumed. Disease development and quarantine of the new Pantoea leaf streak of rice disease caused by P. ananatis on rice need more attention in the near future.

Epidemiological and etiological analysis of two infantile cases of serogroup B epidemic cerebrospinal meningitis epidemics in Shandong Province, 2021

Two cases of epidemic situation of serogroup B meningitis in infants in Shandong Province in 2021 were investigated. Samples of cases and their close contacts were collected for isolation, culture and identification of Neisseria meningitides (Nm). The isolates were subjected to multi-locus sequence typing, outer membrane protein porA and fetA genotyping and drug sensitivity test. Two laboratory-confirmed outbreaks of serogroup B meningitis were reported from Yantai city and Linyi city. The indicated cases were infants aged 5 months and 2 months old respectively. They were not vaccinated with meningitis vaccine. Their epidemiological characteristics and clinical manifestations were similar and the prognosis was good. The same sequence type (ST) of serogroup B Nm strains as the indicated cases was detected in the samples of close family contacts, but without subsequent cases. Among them, Yantai strain was were identified as the type ST-8920, belonging to CC4821 clonal complex, and the genotypes of porA and fetA were p1.21-2, 23 and F3-1. Linyi strain was a new type, belonging to CC4821 clonal complex and the genotypes of porA and fetA were p1.20, 23 and F1-91. The above strains were resistant to penicillin, ciprofloxacin, levofloxacin and Chemitrim, and their sensitivity to cephalosporin decreased. Two cases of infant serogroup B epidemic were relatively rare in China, which were different from the epidemiological and pathogenic characteristics of other Nm serogroups in the past.

Temporal and Spatial Variation in O3 Concentration Near the Surface of Shandong Peninsula and Analysis of Potential Source Areas

The purpose of this study was to explore the temporal and spatial distribution characteristics and potential sources of ozone (O3) in the Shandong Peninsula over a long period of time based on the analysis of the temporal and spatial changes in O3 concentration in Shandong Peninsula from 2005 to 2020. We used wavelet analysis, the entropy weight method, and correlation analysis to discuss O3 and its influencing factors and researched the potential sources of O3 in Shandong Peninsula. The results showed that:① in terms of the time pattern, the near-surface O3 in Shandong Peninsula showed a "triple peak" trend from 2005 to 2020, reaching the maximum value of[(40.48±7.64) μg·m-3] in 2010 and a minimum value of[(36.63±5.61) μg·m-3] in 2013. The season was expressed as:summer[(42.49±1.7) μg·m-3]>spring[(40.65±0.6) μg·m-3]>autumn[(36.47±0.7) μg·m-3]>winter[(36.46±0.3) μg·m-3]. ② In terms of the spatial pattern, the O3 concentration of Shandong Peninsula gradually increased with the increase in latitude from 2005 to 2020, showing the characteristics of high concentrations in the east and west and low in the middle region. During the 16-year evolution of the O3 concentration, there was a 1.5 a main oscillation period. ③The analysis of meteorological conditions revealed that O3 concentration was positively correlated with temperature, precipitation, relative humidity, and sunshine hours, whereas pressure and wind speed were negatively correlated. In the analysis of social factors, soot (dust) emissions were the most obvious factor affecting the third indicator, with a weight of 0.25. ④ Through simulating the trajectory of airflow from different regions (Ji'nan and Qingdao), it was found that the ocean airflow contributed 10.69% to Jinan and 48.94% to Qingdao. There was 64.04% of the long-distance air mass transmission path coming from the northwest, and 43.69% of the short-distance air mass transmission path was from the Bohai Sea and the Yellow Sea, followed by Shandong Province with 21.01%. ⑤ The analysis of potential sources of O3 showed that the potential sources of Ji'nan were mainly distributed in Jinzhou, Liaoning Province, northern Jiangsu Province, Hubei Province, and Anhui Province, with a WPSCF value >0.6, and Qingdao's WPSCF value of >0.6 was mainly distributed in the Yellow Sea area. The O3 contribution of Jining City, Linyi City, Xuzhou City, Huaibei City, and Lianyungang City was >40 μg·m-3. The area with >45 μg·m-3 in Qingdao was mainly in the Yellow Sea. Through the analysis of potential sources in the Shandong Peninsula, particular attention should be paid to the supply of industrial sources in the surrounding areas and the marine sources provided by marine air pollution.