Abstract The elemental micro-segregation characteristic within the weld zone for ytterbium fiber laser welded Hastelloy C-276 sheet was investigated. The analysis of segregation ratio and equilibrium distribution coefficient of elements, determined through EDS data, indicate the reduction in micro-segregation of elements compared with the previous reported literatures for laser welded Hastelloy C-276. High melting efficiency of ytterbium fiber laser, reduction in the amount of linear heat input, and high cooling rate of the mushy zone lead to the reduction in micro-segregation. The melting efficiency of ytterbium fiber laser for welding of Hastelloy C-276 of 64% is higher than that (48%) of conventional welding methods. High melting efficiency leads to the reduction in the linear heat input required for welding. Hence, in the present investigation, the same was found to substantially reduce as compared to the previous reported literature. The cooling rate from liquidus temperature to solidus temperature at the weld centerline was found to be in the order of 103 °C/s. Cellular dendritic substructure that constituted for lower micro-segregation was formed at the weld centerline.