Lime Peel Research Articles

Qualitative Analysis of Pectin Extracted Ultrasonically from Sweet Lime Peel

Qualitative Analysis of Pectin Extracted Ultrasonically from Sweet Lime Peel

Biogenic Synthesis, Characterization and Antimicrobial Activity of Silver Nanoparticles Mediated by Oil Extracted from Waste Seeds of Citrus Sinensis

Nanoparticles have gained significant importance due to their small size and effective surface area. Green synthesis of nanoparticles has become a significant tool in material synthesis due to its eco-friendly, economical and less time-consuming aspects. Sweet lime (Citrus sinensis) peel has been used for extract preparation and synthesis of AgNPs. Such nanoparticles have been shown to have excellent anti-microbial and detoxification properties. Though a large number of studies have shown synthesis of AgNP from peel and other parts of C. sinensis, there are no reports of AgNP synthesis from oil extracted from seeds. In this study, oil was extracted from C. sinensis seeds by solvent extraction. The extracted oil was used for the synthesis of AgNPs by bottom-up path approach. The synthesized AgNPs were found to be spherical in shape with size ranging from 30[Formula: see text]nm and above. SEM and TEM showed that they are uniformly dispersed particles with no aggregation. Different concentrations of AgNO3 (1[Formula: see text]mM, 3[Formula: see text]mM and 10[Formula: see text]mM) showed distinct UV spectra with UV showing sharp peaks for 10[Formula: see text]mM. XRD characterization showed distinct peaks for AgNP when compared to oil extracted. The synthesized AgNPs in this study showed distinct antibacterial activity against E. coli and S. aureus. The AgNPs showed antifungal activity against Pencillium and Rhizopus. Thus, the synthesized AgNPs could be effective antimicrobial agents that could have potential application in food storage and water detoxification.

Development of a Novel Low-Calorie Lime Juice-Based Prebiotic Beverage Using a Combined Design Optimization Methodology.

A novel lime-juice based low-calorie functional beverage was developed by using D-optimal combined design optimization. For the preparation of the beverage, the following functional ingredients were used: lime juice, lime peel essential oil (LEO) as a flavoring agent and bioactive component, sucralose as a low-calorie sweetener, an inulin/polydextrose (I/P) mixture as prebiotic fibers, pectin as a thickening agent and soluble dietary fiber, lutein as a carotenoid colorant and antioxidant, and peppermint extract (ME) as a flavoring agent and bioactive component. A combined design consisting of one mixture factor (LEO/ME ratio), one numeric factor (lutein concentration), and one categoric factor (presence or absence of prebiotics) was used for optimizing the functional beverage based on the sensory quality. Regression models were adequately fitted to the data of sensory acceptance with a determination coefficient >90%. The sample containing a mixture of prebiotics, 2:3 (v/v) ratio of LEO: ME, and 3 mg/100 mL lutein was selected as the best formulation among the six optimal beverages which was suggested by Design-Expert software. This final optimum sample showed the highest total phenolic (44.22 mg gallic acid equivalents/L) and flavonoid (25.49 mg quercetin equivalents/L) contents, and its antioxidant activity (as 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•) scavenging) was 38.30%. The newly designed beverage has the potential to promote health benefits and in therapeutic applications.

Utilization Of Lime Peel Waste (Citrus aurantifolia (Christm.) Swingle) As An Antibacterial Against Salmonella typhi

Typhoid fever is a digestive tract infection caused by Salmonella typhi. Treatment of typhoid fever can use natural ingredients such as lime peel waste. The purpose of this study was to determine the effect of the ethanol extract of lime peel waste on the growth of Salmonella typhi bacteria. The extraction method used the maceration technique, and the antibacterial activity test used the paper disc diffusion method. The results showed that the ethanol extract of lime peel waste at concentrations of 1 mg/ml, 3 mg/ml, 5 mg/ml and 7 mg/ml with inhibition zone diameters of 4.66 mm, 6.55 mm, 9.29 mm and 10.06 mm. The conclusion shows that there is an effect of lime peel ethanol extract in inhibiting the growth of Salmonella typhi bacteria in the weak to strong category.

Understanding association between methylene blue dye and biosorbent: Palmyrah sprout casing in adsorption process in aqueous phase

Water pollution caused by industrial dyes has become a severe problem in the modern world. Biosorbents can be used in an eco-friendly manner to remove industrial dyes. In this study, five biosorbents were selected: palmyrah sprout casing (PSC), manioc peel, lime peel, king coconut husk, and coconut kernel. Batch adsorption experiments were conducted to identify the best biosorbent with the highest ability to adsorb methylene blue (MB) from wastewater. The detailed mechanisms of PSC used in the adsorptive removal of MB in aqueous phase were investigated. Of the five biosorbents, PSC exhibited the best removal performance with an adsorption capacity at equilibrium (qe) of 27.67 mg/g. The qe values of lime peel, king coconut husk, manioc peel, and coconut kernel were 24.25 mg/g, 15.29 mg/g, 10.84 mg/g, and 7.06 mg/g, respectively. To explain the mechanisms of MB adsorption with the selected biosorbents, the Fourier transform infrared (FTIR) spectrometry and X-ray diffraction (XRD) analyses were performed to characterize functional properties, and isotherm, kinetic, rate-limiting, and thermodynamic analyses were conducted. The FTIR analysis revealed that different biosorbents had different functional properties on their adsorptive surfaces. The FTIR and XRD results obtained before and after MB adsorption with PSC indicated that the surface functional groups of carbonyl and hydroxyl actively participated in the removal process. According to the isotherm analysis, monolayer adsorption was observed with the Langmuir model with a determination coefficient of 0.998. The duration to reach the maximum adsorption capacity for MB adsorption with PSC was 120 min, and the adsorption process was exothermic due to the negative enthalpy change (−9.950 kJ/mol). Moreover, the boundary layer thickness and intraparticle diffusion were the rate-limiting factors in the adsorption process. As a new biosorbent for MB adsorption, PSC could be used in activated carbon production to enhance the performance of dye removal.

Extraction of bioactive coumarins from lime peel as sample pretreatment before chromatographic analysis

Abstract Analysis of lime peel was applied to assess their suitability for various intended purposes, e.g., application in perfumery, cosmetics, and cleaning products, or as a source of bioactive or other value-added compounds. Targeted analysis allows wider usability of the waste part of these natural materials. In the present study, a novel, efficient, lab-simple, time-saving analytical method for coumarins determination in lime peel, including sample pretreatment by extraction and quantification by HPLC with fluorescence detection (FLD), is introduced. Optimal conditions of ultrasound assisted extraction included water as extraction solvent, temperature of 40 °C, and extraction time of 10 min. A magnetic molecularly imprinted polymer was employed as solid phase extraction adsorbent for primary extract cleaning, isolation, and enrichment of coumarins before chromatographic analysis. Recovery of herniarin and umbelliferone was more than 86 % ((RSD ≤ 4.8 %). Linear range of 50—1000 ng/mL with correlation coefficient above 0.998 was obtained for the proposed HPLC-FLD method. The limit of quantification was 8.2 and 44 ng/mL for herniarin and umbelliferone, respectively. These results show that the proposed sample pretreatment procedure is suitable for analytical purposes and is perspective also for the analysis of other citrus samples, as well as for future scale-up preparative isolation of bioactive coumarins from citrus peel.

One stage hydrothermal treatment: A green strategy for simultaneous extraction of food hydrocolloid and co-products from sweet lime (Citrus Limetta) peels

The present study is focused on the potential of one stage hydrothermal (HT) treatment using water as a solvent for the simultaneous extraction of pectin and phenols from sweet lime (Citrus Limetta) peels. The effect of temperature (100–120 °C); time (5–25 min) and liquid to solid ratio (LSR) (10–20) were investigated for optimizing the HT process using response surface methodology. The experiments were designed using the Box-Behnken method. The optimum conditions obtained for the highest yield of pectin (23.8%) and TPC (1.18%) were at 112.2 °C, 17.1 min and LSR 14.3. Deviations between predicted and experimental values were statistically insignificant (p > 0.05). The HT process yielded higher pectin and TPC with a significant reduction in extraction time as compared to the conventional extraction process (CSE) i.e., 22.4 and 0.46% of pectin and TPC in 1.5 and 20 h respectively. The HT extracts were characterized at the optimum processing conditions. The degree of esterification (%) and molecular weight (KDa) obtained for HT extract (71.2 ± 1.0 and 330.9) was close to that of commercial citrus pectin (76.6 ± 0.6 and 423.8). The major flavonoids identified (mg/g dw) in HT extract were kaempferol (0.84), rutin (0.82), quercetin (0.71), ferulic (0.46), caffeic (0.4) and gallic (0.31) acid and found to be higher than CSE. The higher antioxidant activity of HT extract was indicated by lower IC 50 (0.62 mg/mL), higher FRAP (37.3 μM/g dw) and ABTS (119.6 μM TE/g dw) values. These results demonstrate the suitability of HT as a one-step green extraction process for simultaneous recovery of pectin and phenols. • Simultaneous extraction of pectin and phenols using hydrothermal (HT) technique. • Citrus Limetta peel was used as substrate and water as a medium of extraction. • Highest pectin (23.8%) and phenol (1.2%) obtained at 112.2 °C, 17.1min and LSR 14.3 • HT yielded high methoxy pectin with molecular weight closer to commercial pectin. • DPPH, FRAP, and ABTS values indicated higher antioxidant potential of HT extract.

Hedonic Ratings and Physicochemical Stability of Antiaging Cream Formulas with Natural Active Ingredients of Nanophytosome from Combination of Merbau Wood-Gotu Kola Leaves Extracts and Essential Oils

Nanophytosome from the combination of Merbau wood (Intsia sp.) and Gotu Kola leaves (Centella asiatica) extracts (NMG) have the potential as natural active ingredients of antiaging creams. This study aims to analyze the preference level of creams with the active ingredient of 4% NMG and the additions of essential oils and to test the physicochemical stability of the most preferred cream. The cream added the essential oil of nutmeg leaf, kaffir lime peel, and lime peel at consentration of 0%, 0.5%, 1%, and 1.5% (w/w). The preference level was measured by hedonic rating tests on the scent, texture, and viscosity by 30 non-standard panelists aged ≥25 years. The most preferred formula was tested for physicochemical stability by storage at low temperature (±4 °C), room temperature (±27 °C), and extreme temperature (±40 °C) for 21 days. The results showed that the most preferred formula was F9, which used 1% kaffir lime peel essential oil. The higher the temperature, the spreadability of the cream will increase; conversely, the higher the storage temperature, the lower the pH value, viscosity and stickiness of the cream, but it still meets the physicochemical standard of cosmetics cream. So this antiaging cream can be used for cosmetic applications.

Influence of sugarcane bagasse fibre on the properties of sweet lime peel- and polyvinyl alcohol-based biodegradable films

Sugarcane bagasse is a readily available agro-waste, it has a potential to be used as a composite material ingredient for development of biodegradable film.

Development of conductive textile fabric using Plackett–Burman optimized green synthesized silver nanoparticles and in situ polymerized polypyrrole

ABSTRACT Electronic textiles (e-textiles) are undergoing rapid technological advancements to attain e-textiles that look and feel like conventional textile fabrics. Research seeks to develop highly functionalized textile-based sensors, actuators, and energy storage devices that integrate seamlessly with current textile technologies. Presently, developments are limited by either low electrical performance, or high cost and complex construction. Additionally, negotiating the balance between high performing e-textiles and environmentally benign production is a challenge. In this report, green synthesized silver nanoparticles (AgNPs) are composited with the conjugated polymer, polypyrrole (Ppy), to create a low-cost conductive textile fabric. A Plackett–Burman design of experiment was used to optimize lime peel extract (LPE) mediated reduction for the synthesis of AgNPs. The results of this optimization process revealed silver nitrate concentration to be a significant factor in both size and UV-vis absorption maxima of the LPE-synthesized AgNPs, and reaction temperature also affecting UV-vis absorption maxima. The resultant optimized AgNPs were consistent in size (40–80 nm) and dispersity (PDI = 0.250). The LPE-synthesized AgNPs are used to form a AgNP-Ppy nanocomposite with a linen textile to produce an e-textile with low electrical resistance (37 Ω).

Formulation and Evaluation of Lime Peel Extract (Citrus aurantiifolia) Gel with Semi-Refined Carrageenan and Glucomannan as Gelling Agent

Lime peel extract contains quercetin with sunscreen activity. It could make into gel preparations, which has a transparent semisolid and generally gives a cooling sensation to the skin. A combination of semi-refined carrageenan and glucomannan as a gelling agent can be an alternative to form the strength and elasticity of the gel mass. This study aimed to determine the effect of concentration variations of gelling agents on the physical evaluation of gel preparation results and the optimal formulation for lime peel extract gel as sunscreen. Lime peel extract would make into gel preparation with variations in the concentration of the gelling agent, which are 1%, 1.25%, 1.5%, and 1.75%. Afterward, the physical evaluation included organoleptic, homogeneity, pH, viscosity, spreadability, and adhesion tests. The organoleptic, homogeneity, pH, and adhesion test results show that all formulations meet the requirements. Viscosity test results obtain the best formula III with 3940,00 cPs at 60 rpm . The results of the dispersibility test show the best formulas I and II with 6,10 cm and 5,50 cm. Based on the data analyzed, variations of gelling agents affect the pH, viscosity, spreadability, and adhesion test results. The optimal lime peel extract gel formulation has not defined from the physical evaluation results.

Comparative Efficacy of Aqueous Extracts of Lime (Citrus aurantifolia) and Grape (Citrus paradisi) Peel in the Control of Callosobruchus maculatus (F.)

A laboratory study was conducted in the Department of Crop Protection, University of Ilorin, to evaluate the single and mixed insecticidal action of aqueous extracts of grape (Citrus paradisi) and lime (Citrus aurantifolia) peel in the control of cowpea beetle, Callosobruchus maculatus. The experiment was laid out in a completely randomized design. There were four treatments with three replicates per treatment. The treatments were applied at the following rates, 0.5ml, 1.0ml, and 1.5ml per 100g of cowpea seeds. The following parameters were used to assess the efficacy of the treatments; adult mortality, oviposition, number of larvae, number of pupae and F1 progeny emergence. The findings of this experiment showed that 1.0 ml of aqueous peel extract of grape + lime had significantly higher female mortality compared to control at 3rd and 5th DAT. Aqueous peel extract of lime caused a significantly lower (70.67 + 4,3) oviposition rate compared to control (141.33+ 7.4) at 2 DAT with 1.5 ml being the most effective rate of application at 4 DAT. Aqueous peel extracts of lime was most effective in significantly reducing larval emergence (1.33 + 0.3) compared to control (8.33 + 0.5). The trend was similar for pupal emergence. Aqueous peel extracts of grape was most effective in reducing male F1 progeny emergence while lime was most effective in reducing female F1 progeny of C maculatus. Aqueous peel extract of lime could be included in control measures used by local farmers in storage of their grains for two months.

The Formulation and Analysis of Solid Bath Soap from Ethanol Extract Of Kaffir Lime Peel (Citrus hystrix DC.) With Several Concentrations

Solid bath soap is a cosmetic preparation to remove dirt from skin surfaces. Kaffir lime peel is a good natural ingredient for skin health. The aim of this study was to produce cosmetic preparation of solid bath soap from a thick extract kaffir lime peel with several concentrations. The thick extract of kaffir lime peel is made by maceration by dissolving 500 grams of powder with 96 % ethanol as solvent as much as 1,000 mL for 2x24 hours and concentrated using a rotary evaporator at 500 C. the method used in soap making is cold processing. The result of the study was a solid bath soap, smelling of fresh orange, bluish color to turquoise blue depends on the concentration of the active substance of kaffir lime extract, soft texture and homogeneous color, Formula 1 with concentration of kaffir lime peel extract of 1 % has pH 10.0, foam height of 5.0 cm, water content was 1.76% and free fatty acids was 2.51%. Formula 2 with concentration was kaffir lime peel extract of 3 % value pH 10.0, foam height of 5.0 Cm, moisture content of 1.95 % and free fatty acids was 2.51 % while formula 3 with an extract of 5 % has a pH 10.0 foam height was 5.0 cm, water content was 0.73 % and free fatty acid was 2.51 %. Solid bath soap with thick extract off kaffir lime peel meets the requirements of SNI number 3532 of 2016 for pH, and moisture content tests while the free fatty acid test does not meet these requirements of SNI

Nano antioxidant serum ethanol extract lime peel (Citrus aurantifolia S.)

Serum is a cosmetic that has received great attention from the industry, but the main challenges are appearance, transparency and public acceptance of herbal active ingredients. On the other hand, herbal cosmetics, such as those using lime peel, are also growing. This study aims to determine the antioxidant activity of lime peel made in a nano extract serum preparation. The method for making nano extracts uses ionic gelation with PSA (Particle Size Analyzer) and Zeta Potential characterization. The concentration of nano serum of lime peel ethanol extract used was 0.5%; 1%; and 3%. Meanwhile, the antioxidant activity testing method uses DPPH and data analysis applied one-way ANOVA then by Tukey test. The results of the nano extract serum formulation using organoleptic testing are transparent, thick and liquid, odorless, and feel moist on the skin. The results of nanoparticle characterization using PSA measurements with three replications were 295.1; 320.3; 385.4 nm and the results of testing the zeta potential of nanoparticles with three replications, namely 3.2; 3.4; 6.0mV. Furthermore, the results of the antioxidant activity test on the basis of the preparation formula are classified as weak (IC50 value of 156.22 μg/mL); Meanwhile, preparation formula with a concentration of 0.5%; 1%; 3% are considered very strong (IC50 value of 46.39 μg/mL; 41.06 μg/mL; 36.27 μg/mL). The results of data analysis consider significance of p value below 0.05. The conclusion is nano lime peel extract meets the requirements and the serum preparation meets the requirements and has antioxidant activity.

Analysis of Molecular Docking Chemical Content of Lime (Citrus aurantiifolia (Christm.) Swingle) Against Diabetes Mellitus Therapy Targets and Prediction of Pharmacokinetic Profiles and Toxicity

Diabetes mellitus drugs currently available are sulfonylureas, biguanides, thiazolidines, and alpha glucoside inhibitors which are widely used to control hyperglycemia. These drugs cannot prevent complications of diabetes, and these drugs should not be used continuously because it causes undesirable pathological conditions. The essential oil in lime peel is rich in phenolics, especially flavonoids, which can prevent oxidative stress. This research was carried out computationally to determine the affinity of the compound mechanism in lime, pharmacokinetic profile, and toxicity of the chemical content of lime which is thought to have antihyperglycemic activity using chemoinformatics studies. The hardware used is an Asus laptop X441UB-GA502T Intel Core I5 – 8250 DDR 4 4GB HD 1TB VGA mix 110 2GB screen 14 "DVD-RW WIN 10 ORI. The software used is PLANTS (PROTEIN-LIGAND ANT SYSTEM), YASARA, Marvin sketch, Swisstargetprediction, SwissADME, and Toxtree. All compounds in lime were most active against the target protein PPARγ with an average value of -78.0092, while the positive control value of thiazolidinediones was -90.3393. The highest inhibitory affinity of the compound contained in lime was hesperidin with the target protein DPP-4 of -113.614, higher than the positive control sitagliptin with an inhibitory affinity value of -107.591. Hesperidin absorption in the digestive tract is low, the topology polar surface area (TPSA) value is 234.29Å2, and low polarity and high lipophilicity. There are unexpected heterocyclic compounds, so it becomes a warning against the potential for genotoxic carcinogenicity, namely oxygen element "o".

Effective control of Alphitobius diaperinus using natural bioinsecticides

Pest insects known as darkling beetles (Alphitobius diaperinus) are common in poultry farms. These pests can develop into a variety of disease vectors, causing damage to chicken coops, decreased chick performance, and financial losses to farmers. In Indonesia, synthetic insecticides are still used for pest control, which is detrimental to non-target creatures and the environment. The purpose of this study is to analyze the effectiveness of biopesticides from cinnamon bark extract (Cinnamomum aromaticum), nutmeg seeds (Myristica fragrans), lavender flowers (Lavandula angustifolia), and lime peel (Citrus aurantifolia), as well as synthetic insecticides alpha-cypermethrin against A. diaperinus larvae and imago. Bioinsecticide levels are 2.5%, 5%, 7.5%, and 10%, while alpha-cypermethrin levels are 1%, 4%, 7%, and 10%, respectively, based on LC50 values. The analysis was repeated three times and the treatment was carried out every two days for a total of 14 days. The data were analyzed with paired sample t-tests to determine the average difference in each treatment when there was an average difference and calculated N-Gain score to determine the effectiveness of biopesticides and synthetic insecticides. The average value of N-Gain essential oil from cinnamon bark extract was successful in causing A.diaperinus death in the imago stage (81.14) and the highest larvae (80) of other test solutions. Cinnamon bark extract and nutmeg seed extract are effective in imago (95.14) and larvae (78.91), respectively, and can be used as a natural biopesticide to replace synthetic insecticides in the control of A.diaperinus in imago and larvae stages. Keywords: Biological control, Biopesticides, Environmental management, Essential oils

Organoleptic and Morphological Analysis of Citrus Peel Powder

Aim: Processing of citrus fruits results in the production of a >40 million tons of by-products worldwide and a major part of this by product is its peels. These peels have the potential of acting as a good nutraceutical resource owing to its high dietary fibre content and phenolic content. However, these peels are under-utilised despite their claimed health benefits. This study evaluates the morphological structure and sensory attributes of sweet lime peel and orange peel powder.
 Study Design: The present study was conducted in 3 phases-
 
 Collection of Raw Material
 Pretreatment & dehydration
 Processing into powder form.
 
 Place and Duration of the Study: The present study was conducted in the Department of Food &Nutrition of Babasa.heb Bhimrao Ambedkar University Lucknow. The duration of the study is 6 months i.e. from Jan, 2022- July, 2022.
 Methodology: Peels of sweet lime and orange were transformed into powder through solar drying followed by grinding. In the present study, we have compared the morphology and organoleptic constituents of orange peel powder (OPP) and sweet lime peel powder (SLPP). The morphology of the two samples was studied by Scanning Electron Microscopy (SEM) and element composition was studied and analysed by Energy Dispersive X-Ray Analysis (EDX). Organoleptic and sensory attributes were analyzed by a 9 pointer hedonic scale rating. Peel powders were also compared for two functional properties i.e. their Solubility Index and Swelling Power.

THE EFFECTIVENESS OF KAFFIR LIME PEEL EXTRACT (Citrus hystrix) ON PLASMA MALONDIALDEHYDE LEVEL IN DEMENTIA MICE

ABSTRACTBackground: Antioxidant is a substance that can catch and neutralize free radicals so that the process of oxidative stress can be stopped and cell damage can be avoided. Kaffir lime peel contains antioxidant compounds that have the potential to be the neuroprotective agent and can protect neurons from free radical-induced damage.Objective: To evaluate the effect of kaffir lime peel extract (Citrus hystrix) on the plasma malondialdehyde (MDA) level of mice induced by Scopolamine (SCM).Method: This research was a true experimental study with a post-test-only controlled group design. Thirty mice were randomly divided into 5 groups consisting of a healthy control (K+), Negative Control (K-), extract 5 mg/20gBW group (P1), 10 mg/20gBW group (P2), 20 mg/20gBW group (P3). SCM was injected intraperitoneally on days 1-7 and kaffir lime peel extract was given orally on days 2-7, the MDA levels testing of mice using TBARS method on day 8. Data analysis used One-Way ANOVA test and continued with Post Hoc LSD test.Results: The mean MDA levels of K+, K-, P1, P2, P3 were 4,212; 4,644; 3,481; 4,555; 4,733 nmol/mL, respectively. MDA levels of P1 were lower than K-, even though there were no statistically significant differences.Conclusion: There was no effect of administration kaffir lime peel extract (Citrus hystrix) on MDA levels in mice with scopolamine-induced dementia.Keywords: Citrus hystrix, malondialdehyde, dementia, mice

(+)‐Limonene‐Lactam: Synthesis of a Sustainable Monomer for Ring‐Opening Polymerization to Novel, Biobased Polyamides

Front Cover: Article 2200185 by Paul N. Stockmann, Malte Winnacker, and co-workers highlights the scalable synthetic route to the novel monomer limonene lactam and its polymer limonene polyamide. The lime peel represents not only the bio-based but also non-food based source of the monomer. Subsequent ring-opening polymerization gives access to a sustainable, “green” material, symbolized by a polymer chain leading into a “principles of green chemistry” seal.

Effects of Lime (Citrus aur ects of Lime (Citrus aurantifolia Swingle) P antifolia Swingle) Peel Extr eel Extract Addition act Addition to Periodontal Dressing on the Number of Blood Vessels in the Gingival Wound Healing Process

Background: Periodontal dressings that are currently available can only protect wounds from mechanical trauma, but have no ability to accelerate wound healing. Lime (Citrus aurantifolia Swingle) peel extract added to periodontal dressing contains flavonoids which can stimulate the formation of new blood vessels, thus accelerating the healing process. Objective: To determine the effects of adding lime (Citrus aurantifolia Swingle) peel extract to periodontal dressing on the number of blood vessels in the gingival wound healing process. Methods: 32 rabbits with 2 mm wound made on the gingiva were divided into 8 groups. For the control groups of K1(n=4) and K2(n=4) periodontal dressings were applied without the addition of lime peel extract. Meanwhile, the treatment groups P1(n=4) and P4(n=4), P2 (n=4) and P5(n=4), and P3(n=4) and P6(n=4), 5%, 10%, and 15% lime peel extract, respectively, were added to the periodontal dressing. Groups K1, P1, P2, and P3 were scarified after 3 days, whereas groups K2, P4, P5, and P6 were scarified after 5 days. Histological observations of tissue by Hematoxylin Eosin (HE) staining were carried out to count the number of blood vessels. Result: The results of the statistical tests show that group P5 had the highest number of new blood vessels. Conclusion: The addition of lime peel extract to periodontal dressing can increase the number of blood vessels in the gingival wound healing process.