The simplest form of description for the distribution, penetration and intensity of daylight is called the Daylight Factor (DF) which results from natural light (sunlight), the DF value is written as a percentage. This value is the ratio of the internal brightness at a point in a room to the natural external brightness on a horizontal surface. As a guide, the brightness inside the building and the associated distribution can be classified according to the natural daylight factor. The necessary natural daylight factors are lighting, glare and thermal comfort. Aspects of classroom lighting and decoration that can promote discomfort and affect task performance due to glare, and flicker (illumination waves above 100 Hz) that cannot be seen from fluorescent lighting. The focus of the study is to measure the level of natural daylight in the classroom. The main purpose of the study was due to students' complaints about the glare and discomfort of the lecture room. The equipment used is lux meter, anemometer and measuring meter. The results of the study show that the lecture room has a level of illumination that meets the standards, glare that cannot be seen and is acceptable for thermal comfort. The sky condition during the study was 100% cloudy with cloud cover. The average illuminance in the lecture room is 276.5lux in a cloudy sky situation (standard 5000-20000 lux) and the daylight factor (DF) of the day is 1.2 in the range of 1.00 to 3.5. The conclusion for this study shows that the daylight factor from natural light in the lecture room at the academic building of Politeknik Kuching Sarawak achieves acceptable standards for lighting, glare and thermal comfort, referring to the MS1525 and CIBSE A standards.