This paper reveals lift-off behavior of jet diffusion flames in sub-atmospheric pressures less than 100kPa, in view of that the current knowledge on this topic is limited for normal pressure conditions. Physically, the variation of ambient pressure may have significant influence on the lift-off behavior of jet diffusion flames due to the change of some critical parameters such as laminar flame speed. In this work, experiments are conducted in a large pressure-controllable chamber of 3m (width)×2m (length)×2m (height) at different sub-atmospheric pressures of 60kPa, 70kPa, 80kPa, 90kPa as well as at normal pressure of 100kPa. Axisymmetric turbulent jet diffusion flames are produced by nozzles with diameters of 4mm, 5mm and 6mm using propane as fuel. It is revealed that the lift-off height increases as the pressure decreases and being much higher than that in normal pressure condition. The laminar flame speed with its dependency on pressure is introduced to interpret such behavior based on classic Kalghatgi model. It is found theoretically that the lift-off height has a power law dependency on pressure by P1−n, where n is overall reaction order of the fuel which is usually larger than 1 indicating a negative power law function with pressure (for example p−0.75 for propane as n=1.75) as well verified by the experimental correlation. Finally, a global model is proposed by including such pressure dependency function into the Kalghatgi model, which is shown to well collapse the experimental results of lift-off heights of different sub-atmospheric pressures.