Life Table Experiments Research Articles

Shifts in competition outcomes between two Daphnia species in response to algal phosphorus content

Competition is one of the key drivers of cladoceran community dynamics. Competitive abilities can be measured as the Threshold Food Concentration (TFC), i.e., concentration of food at which population growth rate equals zero. Species with lower TFC should be superior competitors. However, population TFC can vary with environmental conditions. We hypothesized that algal phosphorus (P) content influences TFC and can alter the outcome of competition between large and small-bodied cladocerans. We conducted competition and life table experiments with single clones of large Daphnia magna and small Daphnia longispina to assess how algal P content affected their TFC and competitive interactions. We also conducted computer simulations to further explore competition between the small and large species. Our experiments showed that TFC varied with the algal P content. P limitation increased the TFC of both species, but this increase was more pronounced for the smaller D. longispina. For this reason, D. magna was a superior competitor at low P content, while D.longispina was superior at high P content. We also found that enhanced food abundance gave an additional advantage to the large-bodied D. magna due to higher reproduction potential at high food concentrations.

Comparative biology of chlorantraniliprole selected and unselected Chrysoperla carnea (Stephens) populations: Stability of resistance, inheritance mode, and realized heritability.

Insecticide resistance in natural enemies can be used as a positive trait in integrated pest management programs by increasing the compatibility of two important tools; biological and chemical control. In this experiment, a field population of Chrysoperla carnea (Stephens) (Neuroptera: Chrysopidae) was selected with chlorantraniliprole for 35 generations (Chlor-Sel) developed a 100.32-fold resistance level compared to an unselected population (Unsel). The dominance values of resistance for reciprocal crosses confirmed incomplete dominance inheritance of chlorantraniliprole resistance. The results on projected rate of chlorantraniliprole resistance showed that at 10% to 90% survival in each selection and a realized heritability value of 0.19, the Chlor-Sel will require only 3 to 26 generations (Slope=1.15) to increase a 10-fold level of resistance. The results of the age-stage life table experiment showed that fecundity, oviposition days, male and female longevity, net reproductive rate (Ro), and gross reproductive rate (GRR) of both crosses were significantly increased compared to their parents. The fecundity of reproductive females, male and female longevity, Ro, generation time (T), and GRR of Chlor-Sel were not significantly different than that of the Unsel strain of C. carnea. In conclusion, chlorantraniliprole resistance in C. carnea has an autosomal, polygenic, and incompletely dominant inheritance mode and C. carnea has potential to develop resistance that leads to fitness benefits. This information will help in management of insect pests by deploying resistant strains with selected insecticides and conservation of these natural enemies in fields with sustaining resistance genes.

Effect of microplastics on the demography of Brachionus calyciflorus Pallas (Rotifera) over successive generations

Microplastics (MPs) are becoming increasingly common in freshwater ecosystems. Rotifers may involuntarily ingest MPs through non-selective filtration, thus causing non-real satiety, leading to adverse effects on their demography. Here, we evaluated the acute and chronic effects of MPs (30 µm) on two successive generations of Brachionus calyciflorus s.s. at 20 and 25 °C. Demographic variables and ingestion rates were compared in the absence and presence of microplastics (550 spheres mL-1). For the life table experiments, cohorts of twenty neonates were introduced into 20 mL medium with four replicates per treatment. Ingestion rates of the rotifer were tested at both test temperatures in the absence and presence of the microplastics in 20 mL containers with 1 ind. mL-1 per container. The rotifer population growth rates ranged from 0.55 to 1.05 d-1 depending on the treatment. MPs decreased the fecundity by 20-24 %. The average lifespan of rotifers exposed to MPs in the F0 generation was lower than that in the F1 generation at both temperatures, indicating that pre-exposure of mothers may offer some protection to the offspring. The population growth rate was higher at 25 °C. The ingestion rates were significantly lower in the presence of the microplastics. Our observations indicated that this strain of B. calyciflorus was better adapted to 20 °C; microplastics and higher test temperature resulted in a pronounced changes in the demographic variables of the rotifer.

A temperature-dependent phenology model for Bemisia tabaci MEAM1 (Hemiptera: Aleyrodidae).

The sweetpotato whitefly, Bemisia tabaci (Gennadius) Middle East-Asia Minor 1 (MEAM1), is widespread across tropical and subtropical regions, affecting hundreds of cultivated and wild plant species. Because the species transmits a variety of viruses, the whitefly has become one of the most economically significant insect pests in the world. Determining a pest's population growth potential as a function of temperature is critical for understanding a species population dynamics, predicting the potential range of the species and its associated diseases, and designing adaptive pest management strategies. The life history of B. tabaci MEAM1 was studied in life-table experiments at 7 constant temperatures ranging from 12 to 35 °C. Nonlinear equations were fitted to development, mortality, and reproduction data and compiled into an overall phenology rate-summation model using Insect Life Cycle Modeling (ILCYM) software, to simulate life-table parameters based on temperature. Life tables of B. tabaci MEAM1 observed at naturally variable temperature in La Molina, Lima, during different seasons, covering the entire temperature range of the species' predicted performance curve, were used to validate the model. Simulations predicted population growth within temperature between 13.9 and 33.4 °C, revealing a maximum finite rate of population increase (λ = 1.163), with a generation time of 33.3 days at 26.4 °C. Predicted species performance agreed well when compared against observed life tables and published data. The process-based physiological model presented here for B. tabaci MEAM1 should prove useful to predict the potential spatial distribution of the species based on temperature and to adjust pest control measures taking different population growth potentials due to prevailing temperature regimes into account.

Urban runoff mortality syndrome in zooplankton caused by tire wear particles

Stormwater runoff from roadways is a global threat to water quality, aquatic organisms, and ecosystems. Tire tread wear particles (TWP) from roadway runoff may lead to urban runoff mortality syndrome (URMS) in some aquatic organisms. We tested the hypothesis that urban runoff from roadways can kill zooplankton. Both roadway runoff and TWP leachate were acutely lethal to a model species, the water flea Daphnia pulex. Life table experiments further revealed the lowered survival rates, intrinsic rate of increase, average life span, and net productive rate of D. pulex when exposed to roadway runoff and TWP leachate. The tire rubber antioxidant N-(1,3-dimethylbutyl)-N'-phenyl-p-phenylenediamine (6PPD) mainly contributed to the TWP toxicity. The toxicity of TWP and 6PPD extracted varied with time in nature. Cladocerans and rotifers were more sensitive to TWP and 6PPD than copepods. These results demonstrate the presence of URMS in zooplankton, which may cascade through food webs and affect aquatic ecosystems.

Copper mediates life history responses of Daphnia pulex to predation threat

A key challenge for ecological and ecotoxicological risk assessment is to predict the risk of organisms when exposed simultaneously to multiple stressors in sub-lethal concentrations. Here, we assessed whether sub-lethal concentrations of an anthropogenic stressors, the heavy metal copper (Cu), mediates the impacts of a natural ecological threat to species, predation risk, among six distinct Daphnia pulex clones. We investigated the interaction between the two stressors on morphological defenses and on several life-history traits including maturation time, size at maturity, somatic growth rate and survival rates. Combining a life table experiment on a response surface design, we found no evidence that the heavy metal copper mediates the effects of predator cue induced morphological responses in the tested D. pulex clones. However, our data indicate that copper can mediate several key life-history responses to predation risk. For age at maturity, we found also clear evidence that the observed interaction between predation risk and copper varied by whether clones were strong or weak morphological responders. Specific exploration of the relationship between morphological responses and life history traits under predation risk and copper suggest a strong hypothesis for multiple strategies to deal with multiple stressors. While interactions between different stressors make it harder to predict their outcomes, and ultimately assess water quality regulations about the effects of such stressors, our study provides evidence that life history theory can aid in understanding and predicting their impacts.

Comparison of diurnal biting activity, life table, and demographic attributes of Aedes albopictus (Asian tiger mosquito) from different urbanized settings in West Java, Indonesia

The Asian tiger mosquito, Aedes albopictus has well-adaptive behavior to environmental changes, including human urbanization, and has an essential role as the main vector of important pathogenic arboviruses. This study aims to analyze the biology and life table of the Ae. albopictus populations collected from urban and peri-urban areas of Bogor, West Java, Indonesia under laboratory conditions. Mosquito eggs collection was carried out in urban and peri-urban areas using ovitraps. The observation of the life table experiment that followed the development of Ae. albopictus started from the emergence of the first individual to the last surviving individual. Several biological parameters comparing Ae. albopictus from two collection sites based on life table analysis were shown to be significantly different. Biting activity of all mosquitoes from urban and peri-urban areas showed a clear bimodal activity with morning peak at 09:00-10.00 and evening peak at 16:00-17:00. Ae. albopictus from the urban area have higher fecundity, considerably longer lifespan, more gonotrophic cycles, and a higher net reproduction rate (R0) than Ae. albopictus from the peri-urban area. These findings will provide valuable information about the well-adapted Ae. albopictus in urban areas and assist in providing basic reproductive data to improve vector control and current surveillance strategies especially in the study area.

Snail juvenile growth rate as a rapid predictor of the transmission potential of parasitizing human schistosomes

Host and parasite traits that are sensitive to environmental perturbations merit special attention in the mitigation of diseases. While life table experiments allow a practical evaluation of variability of these traits with environmental change, they are cost and resource intensive. Here, we use a model snail host-trematode parasite system to test the efficacy of an expeditious alternative. Rapidly changing host traits (such as juvenile growth rate) can be used as effective predictors of parasite transmission potential across a range of environmental factors. This approach can be applied to anticipate epidemiological changes under diverse environmental scenarios.

Assessing the toxicity of polystyrene beads and silica particles on the microconsumer Brachionus calyciflorus at different timescales

Environmental pollution by microplastics has become a severe problem in terrestrial and aquatic ecosystems and, according to actual prognoses, problems will further increase in the future. Therefore, assessing and quantifying the risk for the biota is crucial. Standardized short-term toxicological procedures as well as methods quantifying potential toxic effects over the whole life span of an animal are required. We studied the effect of the microplastic polystyrene on the survival and reproduction of a common freshwater invertebrate, the rotifer Brachionus calyciflorus, at different timescales. We used pristine polystyrene spheres of 1, 3, and 6 µm diameter and fed them to the animals together with food algae in different ratios ranging from 0 to 50% nonfood particles. As a particle control, we used silica to distinguish between a pure particle effect and a plastic effect. After 24 h, no toxic effect was found, neither with polystyrene nor with silica. After 96 h, a toxic effect was detectable for both particle types. The size of the particles played a negligible role. Studying the long-term effect by using life table experiments, we found a reduced reproduction when the animals were fed with 3 µm spheres together with similar-sized food algae. We conclude that the fitness reduction is mainly driven by the dilution of food by the nonfood particles rather than by a direct toxic effect.

Combined Effects of Warming and Imidacloprid on Survival, Reproduction and Population Growth of Brachionus calyciflorus (Rotifera).

Global warming and pesticide contamination are two stressors of high concern, but their combined effects on freshwater biota are controversial. This study investigated the combined effects of warming and imidacloprid (IMI) on survival (measured as life expectancy at hatching), reproduction (net reproductive rate), population growth (intrinsic rate of population increase) and sexual reproduction (proportion of sexual offspring) of Brachionus calyciflorus using a life table experiment. The results showed that compared with controls, treatments with IMI at 50-100mg/L significantly decreased survival, reproduction and population growth of the rotifers at 20℃. The inhibiting effect at higher IMI concentrations on survival increased with increasing temperatures, but those on reproduction and population growth increased only when the temperature increased from 25℃ to 30℃. The proportion of sexual offspring decreased with increasing temperatures. When monitoring the ecological effects of pollutants, environmental temperature and the possible adaptation of rotifers to it should be taken into consideration.

Food quantity and quality shapes reproductive strategies of Daphnia.

In freshwater environments, one of the challenges aquatic grazers face are periods of suboptimal food quantity and quality. In a life table experiment, the effects of food quantity (a gradient of algae concentration) and quality (a diet of cyanobacteria) on the life histories and resource allocation strategy in Daphnia magna were tested. Growth‐related traits were similarly affected under different food regimes while the reproductive strategies differed in animals exposed to low food quantity and quality. The per‐clutch investment (clutch volume) did not differ between Daphnia fed with cyanobacteria and underfed mothers, but resources were differently allocated; underfed mothers increased their per‐offspring investment by producing fewer, but larger eggs, whereas cyanobacteria‐fed mothers invested in a greater number of eggs of smaller size. I argue that both strategies of resource allocation (number vs. size of eggs) may be adaptive under the given food regime. The results of the study show that the cyanobacteria diet‐driven fitness losses are comparable to losses caused by food quantity, which is only slightly above the growth capability threshold for Daphnia.

Competitive consequences determined by phenotypic but not genetic distance: A study with asexual water flea genotypes

Abstract How evolutionary relatedness influences the strength of competitive interactions among genetically isolated populations has been a long‐standing interest in ecology. Darwin's ‘Competition‐Relatedness Hypothesis (CRH)’ states that, since closely related species should compete more strongly, they are less likely to coexist, while Herbert's ‘Bimodal Competition Hypothesis (BCH)’ predicts that competitive exclusion is less likely to occur when the competing species are genetically close or distant. To test these hypotheses, we experimentally examined the difference in the competitive ability and life tables of fecundity and survivorship among four different genotypes of asexual Daphnia cf. pulex that diverged from a single ancestral genotype. The experiments showed that the competitive consequences differed depending on the pairing of the competing genotypes, and that the degree of the competitive exclusion was lower when the competing genotypes were genetically closer to each other. These results partially supported the BCH but not the CRH at all. More importantly, the degree of competitive exclusion was better predicted by the phenotypic rather than genetic distances between the competing genotypes. The life table experiments revealed that competitively inferior genotypes had higher early reproduction rates, but survival rates decreased with age and thus body size, probably a result of selection by predation pressures found in nature. These results indicate that competitive superiority is highly dependent on selection pressures that given organisms have been evolutionally subjected to, and, that genetic similarity is not necessarily an appropriate measure for predicting the completive exclusion on an ecological time‐scale. To predict competitive relationships among the organisms, it is essential to comprehend their phenotypic differences rather than simply knowing their genetic or phylogenetic relationships. Read the free Plain Language Summary for this article on the Journal blog.

Rapid adaptation of Brachionus dorcas (Rotifera) to tetracycline antibiotic stress.

Although natural populations can rapidly adapt to selection pressures, the fitness consequences of selection are controversial. In this study, a selection experiment was conducted with replicate populations of Brachionus dorcas that were exposed to two sublethal concentrations (26.8 and 78.3mg/L) of oxytetracycline (OTC), followed by two common garden experiments (population growth and life table experiments). During the 102-day (approximately 36 asexual generations) selection experiment, a markedly increased growth rate but a significantly decreased mictic ratio over time in the populations exposed to OTC when compared to the control populations suggested that the former adapted to the selection pressures and that a trade-off exists between asexual and sexual reproduction. The high and stable population growth rates after 90 days of OTC selection illustrate an example of evolutionary rescue. After 102 days of selection, OTC-selected populations showed higher population growth rates than the control populations when exposed to OTC, indicating significantly increased tolerance. OTC-selected populations showed a lower average growth rate, longer average generation time and life expectancy at hatching, and higher average net reproduction rate and proportion of mictic offspring than the control populations in the absence of OTC, which indicate that OTC selection results in two fitness costs and three fitness gains and that the effect of OTC selection on fitness differs with the measured fitness variables. Both the evolutionary potential of populations under the stress of higher concentrations of OTC and the fitness costs and gains of selection in the absence of OTC indicate that past exposures to pollutants cannot be neglected when evaluating the effects of current stressors on natural populations.

Effects of Guangzhou seasonal climate change on the development of Aedes albopictus and its susceptibility to DENV-2.

The susceptibility of Asian tiger mosquitoes to DENV-2 in different seasons was observed in simulated field environments as a reference to design dengue fever control strategies in Guangzhou. The life table experiments of mosquitoes in four seasons were carried out in the field. The susceptibility of Ae. albopictus to dengue virus was observed in both environments in Guangzhou in summer and winter. Ae. albopictus was infected with dengue virus by oral feeding. On day 7 and 14 after infection, the viral load in the head, ovary, and midgut of the mosquito was detected using real-time fluorescent quantitative PCR. Immune-associated gene expression in infected mosquitoes was performed using quantitative real-time reverse transcriptase PCR. The hatching rate and pupation rate of Ae. albopictus larvae in different seasons differed significantly. The winter hatching rate of larvae was lower than that in summer, and the incubation time was longer than in summer. In the winter field environment, Ae. albopictus still underwent basic growth and development processes. Mosquitoes in the simulated field environment were more susceptible to DENV-2 than those in the simulated laboratory environment. In the midgut, viral RNA levels on day 7 in summer were higher than those on day 7 in winter (F = 14.459, P = 0.01); ovarian viral RNA levels on day 7 in summer were higher than those on day 7 in winter (F = 8.656, P < 0.001), but there was no significant difference in the viral load at other time points (P > 0.05). Dicer-2 mRNA expression on day 7 in winter was 4.071 times than that on day 7 in summer: the viral load and Dicer-2 expression correlated moderately. Ae. albopictus could still develop and transmit dengue virus in winter in Guangzhou. Mosquitoes under simulated field conditions were more susceptible to DENV-2 than those under simulated laboratory conditions.

Artificial Light Pollution with Different Wavelengths at Night Interferes with Development, Reproduction, and Antipredator Defenses of Daphnia magna.

Artificial light at night (ALAN) dramatically alters the natural daily cycle of light and may cause a suite of physiological and behavioral responses of freshwater crustacean Daphnia. Here, we conducted a life table experiment to investigate the effect of different wavelengths [white (L-L, 400-800 nm), red (R, 614 nm), green (G, 527 nm), and blue (B, 447 nm)] and flashing modes [three colors flash alternately (3-Flash), seven colors strobe alternately (7-Strobe)] of ALAN on the development, reproduction, and predator-induced defenses of Daphnia magna. The results showed that G inhibited the development of the body and tail spine of Daphnia at sexual maturity and decreased their reproduction. The 7-Strobe promoted Daphnia to develop a larger eye. There was an interaction between fish kairomones and ALAN, which intensified over time. ALAN enhanced the response of the relative tail spine length to fish kairomone at sexual maturity. In addition, L-L and R inhibited the body length of 10th instar D. magna in response to fish kairomone. Through the hierarchical cluster method, the 3-Flash and B are recommended as friendly artificial lighting to Daphnia. Our results highlight the interference effects of ALAN on Daphnia, which are helpful to assess the potential impact of light pollution on zooplankton.

Exposure to toxic Microcystis via intact cell ingestion and cell crude extract differently affects small-bodied cladocerans

Cyanobacterial blooms are increasingly common in aquatic environments worldwide. These microorganisms cause concern due to their ability to produce cyanotoxins. Aquatic organisms, especially zooplankton, are exposed to cyanobacterial toxins by different routes, depending on the bloom phase. During cyanobacterial dominance, zooplankton is exposed to cyanotoxins through the ingestion of cyanobacterial cells, while at the bloom senescence, dissolved toxins are the most representative route. In this study, we assessed the effects of a microcystin-producing strain of Microcystis aeruginosa (NPLJ-4) on clones of the tropical small cladocerans Macrothrix spinosa (two clones) and Ceriodaphnia cornuta (one clone) exposed to intact cells and aqueous cell crude extracts. Short-term toxicity assays and life-table experiments were performed to assess the effects of the toxic M. aeruginosa on the survival and life history of the cladocerans. In the short-term toxicity assay, we found that cladocerans were more affected by intact cells. Both clones of M. spinosa were more affected when exposed to intact cells, while C. cornuta displayed about 5-fold more resistance. On the other hand, crude extracts had a low impact on cladocerans' survival. Also, we observed a significant decrease in survival, fecundity, and growth of animals exposed to sublethal and environmentally relevant concentrations of M. aeruginosa cellular biomass. However, even at high concentrations of dissolved microcystins, the crude extract did not have significant effects on the life history parameters of the cladocerans. Although they can be found during cyanobacterial bloom events, small-bodied cladocerans are still affected by toxic Cyanobacteria depending on the exposure route.

Aedes albopictus life table: environment, food, and age dependence survivorship and reproduction in a tropical area

BackgroundEnvironmental conditions affect the biology of mosquito vectors. Aedes albopictus is a major vector of many important diseases including dengue, Zika, and chikungunya in China. Understanding the development, fecundity, and survivorship of Ae. albopictus mosquitoes in different environmental conditions is beneficial for the implementation of effective vector control measures.MethodsAedes albopictus larval and adult life-table experiments were conducted under natural conditions in indoor, half-shaded, and fully shaded settings, simulating the three major habitat types in Hainan Province, a tropical island in the South China Sea. Temperature, humidity, and light intensity were recorded daily. Larval rearing used habitat water and tap water, with and without additional artificial food. Development time, survivorship, pupation rate, and adult emergence rates were monitored. Adult mosquito survivorship and fecundity were monitored daily and reproductive rates were determined, and age-dependent survivorship and reproduction were analyzed.ResultsThe pupation time and male and female emergence times were significantly shorter in indoor conditions than in shaded and half-shaded conditions for both tap water with added food and habitat water with added food groups. For habitat water with added food, the shaded environment had the lowest pupation rate among the settings. For tap water with added food group, the shaded environment had the lowest pupation rate. The mean survival time of females was 27.3 ± 0.8 days in the indoor condition, which was significantly longer than that in the half-shaded (18.4 ± 0.6 days) and shaded (13.8 ± 1.2 days) conditions. Adult mortality was age-dependent, and the rate of change in mortality with age was not significantly different among different environmental conditions. The mean net replacement rate (R0) of female mosquitoes showed no significant difference among the three conditions, whereas the per capita intrinsic growth rate (r) in the shaded condition was 42.0% and 20.4% higher than that in the indoor and half-shaded conditions, respectively. Female daily egg mass was also age-dependent in all the settings, decaying exponentially with age.ConclusionsOur results imply that half-shaded conditions are likely the best natural condition for adult emergence and female reproduction, and food supply is crucial for larval development and pupation. The results provide new avenues for integrated mosquito management in indoor and outdoor areas, especially in half-shaded areas.Graphical

Effect of elevated temperature on the population dynamics of fall armyworm, Spodoptera frugiperda

Aim: The present study was conducted to determine the growth, survival, fecundity and mortality rate of Spodoptera frugiperda in maize at elevated temperature. Methodology: Biology and life table experiments were carried out in custom made Open Top Chambers (4m x 4m x 4m size) fitted with SCADA under increasing temperature regimes viz., 32˚C, 33˚C, 34˚C, 35˚C and 36˚C. Each stage of the insects was examined daily and the fertility and life-table parameters were calculated by using TWOSEX-MS chart. Results: The overall developmental time for egg, larva, pupa and adult stages (total lifespan) at different elevated temperatures were in the order of 32˚C &gt; 33˚C &gt; 34˚C &gt; 35˚C &gt; 36˚C. The fecundity rate of S. frugiperda at different elevated temperatures were in the order of 34˚C &gt; 33˚C &gt; 32˚C &gt; 35˚C &gt; 36˚C. Interpretation: The current study confirms that the elevated temperature regimes had a positive correlation with fecundity rate upto 34˚C and negative correlation with the survival rate of S. frugiperda.

Survivorship of Anopheles gambiae sensu lato in irrigated sugarcane plantation scheme in Ethiopia

BackgroundTo ensure food security, sub-Saharan Africa has initiated massive water resource development projects, such as irrigated agriculture, in recent years. However, such environmental modifications affect the survivorship and development of mosquitoes, which are vectors of different diseases. This study aimed at determining the effects of irrigation practices on development and survivorship of Anopheles gambiae s.l. in Ethiopia.MethodsA life table experiment was conducted to examine the effect of environmental modification on survivorship of both immature and adult An. gambiae s.l. in irrigated and non-irrigated areas. The pupation rate and development time of the immatures and adult longevity and fecundity were compared between the two settings.ResultsThe estimated mean survival time of female An. gambiae s.l. in the irrigated and non-irrigated areas was 37.9 and 31.3 days, respectively. A survival analysis showed that adult females of An. gambiae s.l. placed in an irrigated area lived significantly longer than those in a non-irrigated area (χ2 = 18.3, df = 1, P <0.001), and An. gambiae s.l. females lived significantly longer than males in both areas (P < 0.001).ConclusionsAdult An. gambiae s.l. survivorship was found to be enhanced in the irrigated area compared to non-irrigated area. Longer survival of adult mosquitoes in irrigated areas could have important implications for vectorial capacity and hence malaria transmission.Graphical

Long-term effects of elevated pCO2 levels on the expression of Chaoborus-induced defences in Daphnia pulex

Increased carbon dioxide from fossil fuel combustion results in an enrichment of CO2 in the global carbon cycle. Recent evidence indicates that rising atmospheric CO2 impacts the partial pressure of carbon dioxide (pCO2) in freshwaters. This affects freshwater biota by disrupting chemical communication between predator and prey. One such well-described predator-prey interaction is the phantom midge larva Chaoborus preying on the freshwater crustacean Daphnia pulex. To counter Chaoborus predation, D. pulex develops defensive neckteeth in response to chemical cues. The strength of neckteeth expression is reduced when D. pulex experience elevated pCO2 levels. This is discussed to directly impair predator perception and results in reduced defence expression. However, it is not known whether there are also long-term effects associated with continuous elevated pCO2. Here, we investigated the effect of long-term exposure of D. pulex to elevated pCO2 levels in a life-table experiment over three generations. Using a flow-through system, we continuously exposed D. pulex to cues released by the predatory larva Chaoborus and control or elevated pCO2 levels. We determined morphological defence expression in the 2nd juvenile instar and the number of neonates as a measure for life-history traits over three successive generations. We detected that elevated pCO2 significantly reduces the expression of predator-induced morphological defences (i.e. neckteeth) and life-history parameters (i.e. number of neonates) in successive generations. Our data clearly show that at least three generations become more vulnerable to predation without indications of transgenerational acclimation. As Daphnia is a keystone grazer of freshwater ecosystems, this may destabilise population growth rates. In conclusion, long-term effects of pCO2-induced reduction of predator-induced plasticity may significantly affect trophic interactions.