Life History Research Articles

Variation in the Local Grey Mullet Populations (Mugil cephalus) on the Western Pacific Fringe

Background: Understanding population genetic structures is crucial for planning and implementing conservation programmes to preserve species’ adaptive and evolutionary potential and thus ensure their long-term persistence. The grey mullet (Mugil cephalus) is a globally distributed coastal fish. Its populations in waters surrounding Taiwan on the western Pacific fringe are divided into at least two stocks (migratory and residential), but questions remain regarding their genetic divergence and gene flow. Methods and Results: To cast more light on this, allozyme variations at 21 presumptive gene loci of 1217 adult grey mullets from 15 localities in Japan, Taiwan and mainland China, and four gene loci from 1470 juveniles from three localities in Taiwan were used to investigate patterns of genetic variation. The mean expected heterozygosity (He) was 0.128—ranging from 0.031 (Matsu) to 0.442 (Kaoping)—and the mean observed heterozygosity (Ho) was 0.086—ranging from 0.017 (Kaohsiung) to 0.215 (Kaoping). Both AMOVA and the high overall mean FST of 0.252 indicated enormous genetic differentiation among populations and the positive mean value of FIS was 0.328, indicating a deficiency of heterozygotes. PCoA indicated that the samples of M. cephalus could be split into three groups and STRUCTURE analysis showed that all individuals were grouped into three genetic clusters. The results of mutation-drift equilibrium tests did not suggest that the populations experienced any recent genetic bottleneck. The results from all localities in the present investigation showed significant change in the GPI-A genotype frequencies with latitudes—e.g., increases in GPI-A*135/135 homozygote frequencies and GPI-A*100/100 frequencies were highly correlated with latitudinal cline. All migratory populations with the GPI-A genotype were almost exclusively the GPI-A*100/100 homozygote. During the life history of M. cephalus, the GPI-A*100/135 heterozygote frequency significantly decreases with age. Conclusions: Based on these data, we suggest that each GPI-A genotype represents trait combinations of higher fitness in some portions of the environment. Furthermore, the genotypic frequencies change in accordance with life stages, suggesting that selection occurs throughout the life span.

Patterns of Ecological Specialisation Among Multiple Piscivorous Morphs of a Diverse Assemblage of Salvelinus Fishes

ABSTRACTAdaptive radiation is considered to be a major source of biodiversity in charrs of the genus Salvelinus. These fishes demonstrate numerous examples of diversification in postglacial lakes and rivers resulting in the emergence of different ecomorphs, including piscivorous. Usually, the number of fish feeders does not exceed two, suggesting the existence of ecological and/or internal factors that prevent further diversification. Under these circumstances, rare instances of increases in the number of piscivorous morphs are of particular interest. Here, we present a comprehensive study of the piscivorous charr assemblage in Lake Kronotskoe, residence of the most diverse species flock of Salvelinus fishes. To unravel the diversity and ecological relationships of piscivorous charrs, we analysed their morphology and microsatellite DNA polymorphism, distribution within the lake, trophic preferences and parasite load, as well as trace element accumulation, stable isotope and free fatty acid ratios. We revealed that, apart from a few strictly benthivorous morphs, the lake is inhabited by four closely related but morphologically and genetically distinct morphs that exhibit a range of piscivorous specialisation: from opportunistic secondary predation to ultra‐specialised primary piscivory. They effectively exploit the spatiotemporal structure of the ecosystem and experience diversifying selection due to niche discordance. Each piscivorous morph develops specific phenotypic, ecological and life history traits that allow it to terminate weakly overlapping branches of the trophic chain and share the energy flows of the lake ecosystem. Our data suggest that Lake Kronotskoe facilitates high level of ecological opportunity that favours niche expansion and allows the piscivorous lineage to simultaneously realise the archetypical modes of ecological diversification available to fishes in cold‐water freshwaters.

Germination responses to changing rainfall timing reveal potential climate vulnerability in a clade of wildflowers.

The seasonal timing of life history transitions is often critical to fitness, and many organisms rely upon environmental cues to match life cycle events with favorable conditions. In plants, the timing of seed germination is mediated by seasonal cues such as rainfall and temperature. Variation in cue responses among species can reflect evolutionary processes and adaptation to local climate and can affect vulnerability to changing conditions. Indeed, climate change is altering the timing of precipitation, and germination responses to such change can have consequences for individual fitness, population dynamics, and species distributions. Here, we assessed responses to the seasonal timing of germination-triggering rains for eleven species spanning the Streptanthus/Caulanthus clade (Brassicaceae). To do so, we experimentally manipulated the onset date of rainfall events, measured effects on germination fraction, and evaluated whether responses were constrained by evolutionary relationships across the phylogeny. We then explored the possible consequences of these responses to contemporary shifts in precipitation timing. Germination fractions decreased with later onset of rains and cooler temperatures for all but three Caulanthus species. Species' germination responses to the timing of rainfall and seasonal temperatures were phylogenetically constrained, with Caulanthus species appearing less responsive. Further, four species are likely already experiencing significant decreases in germination fractions with observed climate change, which has shifted the timing of rainfall towards the cooler, winter months in California. Overall, our findings emphasize the sensitivity of germination to seasonal conditions, underscore the importance of interacting environmental cues, and highlight vulnerability to shifting precipitation patterns with climate change, particularly in more northern, mesic species.

From RNA to DNA: Emergence of reverse transcriptases from an ancestral RNA-dependent RNA polymerase

The transition from RNA as the informational molecule of primordial biological systems to the DNA genomes of modern organisms represents one of the greatest evolutionary transitions in the history of life. One way to understand this transition is to comprehend the origin of the enzymes responsible for the metabolism of nucleic acid polymers. In the present work, we reconstructed the ancestral sequence of RNA-dependent DNA polymerase (RdDp) and modeled its structure. The data demonstrate that, in terms of primary sequence, the ancestral sequences exhibit characteristic elements of RdDp; however, structurally, they are more similar to RNA-dependent RNA polymerase (RdRp). The presented data suggest that RdDp may have originated through modifications and neofunctionalization from an RdRp-like ancestor.

Palaeohistology of Macrospondylus bollensis (Crocodylomorpha: Thalattosuchia: Teleosauroidea) from the Posidonienschiefer Formation (Toarcian) of Germany, with insights into life history and ecology.

The Posidonienschiefer Formation of southern Germany has yielded an array of incredible fossil vertebrates. One of the best represented clades therein is Teleosauroidea, a successful thalattosuchian crocodylomorph group that dominated the coastlines. The most abundant teleosauroid, Macrospondylus bollensis, is known from a wide range of body sizes, making it an ideal taxon for histological and ontogenetic investigations. Previous studies examining thalattosuchian histology provide a basic understanding of bone microstructure in teleosauroids, but lack the taxonomic, stratigraphic, and ontogenetic control required to understand growth and palaeobiology within a species. Here, we examine the bone microstructure of three femora and one tibia from three different-sized M. bollensis individuals. We also perform bone compactness analyses to evaluate for ontogenetic and ecological variation. Our results suggests that (1) the smallest specimen was a young, skeletally immature individual with well-vascularized-parallel-fibered bone and limited remodeling in the midshaft periosteal cortex; (2) the intermediate specimen was skeletally immature at death, with vascularized parallel-fibered bone tissue interrupted by at least 10 LAGs, but no clear external fundamental system (EFS), and rather extensive inner cortical bone remodeling; and (3) the largest specimen was skeletally mature, with parallel-fibered bone tissue interrupted by numerous LAGs, a well-developed EFS, and extensive remodeling in the deep cortex. Macrospondylus bollensis grew relatively regularly until reaching adult size, and global bone compactness values fall within the range reported for modern crocodylians. The lifestyle inference models used suggest that M. bollensis was well adapted for an aquatic environment but also retained some ability to move on land. Finally, both larger specimens display a peculiar, localized area of disorganized bone tissue interpreted as pathological.

Morphological and Phylogenetic Analyses Reveal Dictyostelids (Cellular Slime Molds) Colonizing the Ascocarp of Morchella.

Morchella spp. (true morels) are precious edible mushrooms consumed around the world, with a delicious taste, rich nutritional value, and unique healthcare effects. Various fungi and bacteria have been reported to colonize the ascocarps of Morchella, damaging their fruiting bodies and leading to serious economic losses in cultivation. The species identification of these colonizing organisms is crucial for understanding their colonization mechanisms on morels. Slime molds, which have characteristics of both "fungi" and "animals", can occasionally colonize crops and edible fungi. However, there have been no reports of dictyostelid cellular slime molds (dictyostelids) colonizing plants and fungi to date. In this study, we discovered that dictyostelids colonized the surface of one wild ascoma of Morchella in the forest of Chongqing, China, with the tissues being black and rotten. Macro- and micro-morphological observations, along with molecular phylogenetic analyses, identified the specimens investigated in this study as Dictyostelium implicatum and Morchella sp. Mel-21. The results provide new knowledge of dictyostelid colonization on organisms and contribute to the diversity of species colonizing true morels. Moreover, this is also the first report of dictyostelids distributed in Chongqing, China. This study enhances our insights into the life history and potential ecological significance of dictyostelids and updates their distribution area in China. Further research will be conducted to uncover the mechanisms behind the colonization observed in this study.

Side effects of semi-synthetic insecticide spinetoram on the whitefly parasitoid Encarsia formosa.

Insecticide risk assessment for biological control agents is essential for implementing integrated pest management (IPM) programs. Spinetoram is a naturally derived insecticide used to control a variety of agricultural sap-sucking insect pests, and Encarsia formosa Gahan (Hymenoptera: Aphelinidae) plays a key role in the biocontrol of greenhouse whiteflies. Despite its presumed safe ecotoxicological profile, the side effects of spinetoram on E. formosa are partially unknown, especially at sublethal doses. In the laboratory, we estimated the lethal concentrations (LC) of spinetoram on E. formosa by topical exposure of pupae and 48 h residual contact exposure of emerged adults. Afterwards, we assessed the impact of spinetoram LCs on the life history traits (juvenile development, adult longevity, parasitism, adult emergence) and population growth parameter (the instantaneous rate of increase, ri) of the parasitoid. Probit analysis allowed the estimation of LC10 = 0.10 mg/L, LC50 = 0.56 mg/L, and LC90 = 3.28 mg/L. Spinetoram at LC10 had no impact on the observed parameters of E. formosa. Conversely, spinetoram at LC50 and LC90 significantly reduced adult longevity by 19.2% and 40.6%, total parasitism by 33.4% and 38.7%, and total adult emergence by 29.6% and 39.1%, respectively. Also, the ri of E. formosa was significantly affected by spinetoram LC50 and LC90 with a reduction of 8.17 and 29.83%, respectively. Our results suggested that spinetoram significantly threatens the effectiveness of E. formosa when applied at the field recommended rate. However, further validation of spinetoram under greenhouse conditions is required for a comprehensive risk assessment for E. formosa in IPM programs. © 2024 Society of Chemical Industry.

Brain gene expression reveals pathways underlying nocturnal migratory restlessness

Migration is one of the most extreme and energy demanding life history strategies to have evolved in the animal kingdom. In birds, champions of long-distance migrations, the seasonal emergence of the migratory phenotype is characterised by rapid physiological and metabolic remodelling, including substantial accumulation of fat stores and increases in nocturnality. The molecular underpinnings and brain adaptations to seasonal migrations remain poorly understood. Here, we exposed Common quails (Coturnix coturnix) to controlled changes in day length to simulate southward autumn migration, and then blocked the photoperiod until birds entered the non-migratory wintering phase. We first performed de novo RNA-Sequencing from selected brain samples (hypothalamus) collected from birds at a standardised time at night, either in a migratory state (when restlessness was highest and at their body mass peak), or in a non-migratory state and conducted differential gene expression and functional pathways analyses. We found that the migratory state was associated with up-regulation of a few, yet functionally well defined, gene expression networks implicated in fat trafficking, protein and carbohydrate metabolism. Further analyses that focused on candidate genes (apolipoprotein H or APOH, lysosomal associated membrane protein-2 or LAMP2) from samples collected during the day or night across the entire study population suggested differences in the expression of these genes depending on the time of the day with the largest expression levels being found in the migratory birds sampled at night. We also found that expression of APOH was positively associated with levels of nocturnal activity in the migratory birds; such an association was absent within the non-migratory birds. Our results provide novel experimental evidence revealing that hypothalamic changes in expression of apolipoprotein pathways, which regulate the circulatory transport of lipids, are likely key regulatory activators of nocturnal migratory movements. Our study paves the way for performing deeper functional investigations on seasonal molecular remodelling underlying the development of the migratory phenotype.

Factors Associated to the Presence of Isthmocele Diagnosed by Pelvic Ultrasound, Magnetic Resonance Imaging or Diagnostic Hysteroscopy: A Cross-Sectional Study.

We aimed to analyze the prevalence of isthmocele and factors associated with its onset within a cohort of women with previous history of cesarean section. A cross-sectional study with 90 women assessed from 2020 to 2022. Isthmocele was a composite variable diagnosed by transvaginal ultrasound (TVUS), magnetic resonance imaging (MRI) and/or diagnostic hysteroscopy (DxHys) and were asked about clinical symptoms, sociodemographic and obstetrical history and quality of life by the WHO-QOL questionnaire. Univariate and multivariate analysis (odds ratio (OR) plus 95% confidence intervals-CI) were performed to seek factors associated with the presence of isthmocele (5% significance level).The prevalence of isthmocele after combining MRI, TVUS and DxHys was 63.3% (n = 57). Women with isthmocele presented a higher body mass index (BMI) measured during delivery (32.70 ± 6.07 vs. 28.28 ± 9.86kg/m2;p < 0.05) than women without isthmocele. Other sociodemographic variables, obstetrical history and WHO-QOL subdomains did not differ between groups. Within women with isthmocele, the residual myometrial mantle had an average of 4.97 ± 1.57cm. Uterine volume was higher in the isthmocele group (103.95 vs. 81.34 cm3; p = 0.08), but with no statistical difference. Multivariate analysis (logistic regression) has reported that the factors associated with isthmocele were: higher BMI during delivery (aOR = 1.26[1.07-1.49];p < 0.05); longer interpartum interval (aOR = 1.22[1.03-1.46];p = 0.02) and presence of more than two cesarean sections (aOR = 2.16[1.16-4.01];p = 0.02). We concluded that a high prevalence of isthmocele was found. Women with previous cesarean section, with higher BMI during delivery and longer interdelivery interval were risk factors for the presence of isthmocele.

Toward sustainability: mechanism between work–family balance and fertility intention with decent work as the mediating role

The relationship between work and family is essential to sustainability, affecting the fertility intention and fertility behavior of childbearing age. This study aims to explore the following research questions to fill the research gaps: (1) Can WFB improve individuals’ fertility intentions (FI)? (2) How does DW affect individuals’ FI? Drawing on the job demands-resources theory (JD-R) and life history theory, we have developed a theoretical framework of work–family balance (WFB), decent work (DW), fertility intention (FI), organizational support (OS), and family support (FS). Data was collected through a questionnaire in China, and hypotheses were tested. We find that WFB significantly and positively affects DW; DW significantly positively affects FI; WFB significantly influences FI, and DW is a mediator between WFB and FI. However, OS and FS have no significant moderating effects on WFB and DW. The theoretical implications of this study are that it preliminarily explores the spillover effects of DW on family life, enriching the research on DW. The findings can be applied to formulating government policies on fertility support, human resource management practices, and personal work–family development.

Inferring the origin of new D-loop haplotypes of loggerhead sea turtles (Testudinata: Cheloniidae) from the Southwest Atlantic lineage

The populations of the loggerhead turtles, Caretta caretta, present four main D-loop mitochondrial haplogroups that are distributed across the Indo-Pacific, Mediterranean, and Atlantic oceans. The Southwestern Atlantic (SWA) is one of the Regional Management Units (RMUs) of loggerheads, characterized by unique haplotypes, high nest density, and distinct life history traits. Detecting new D-loop haplogroups is important, particularly endemic ones, as they can enhance our understanding of their life history within the RMUs and contribute to the resolution of mixed stock analysis. In this study, we conducted a series of phylogenetic delimitation and network analyses to identify, validate, and infer the origin of four new D-loop haplotypes detected in the loggerhead populations from the SWA. Our findings demonstrate that these new D-loop haplotypes are valid and unique to the SWA lineage, potentially aiding in the delimitation of individuals’ origins and the inference of their lineage.

Evaluating the Effects of Epichloë Fungal Endophytes of Perennial Ryegrass on the Feeding Behaviour and Life History of Rhopalosiphum padi.

The bird cherry-oat aphid, Rhopalosiphum padi (L.), is an economically significant pest of pasture grasses, the latter being capable of hosting several fungal endophyte-perennial ryegrass symbiota rich in alkaloids and toxic to vertebrates and invertebrates. Measuring aphid feeding behaviour can provide insights into the effectiveness and mode of action of different fungal endophytes. This study investigated the effects of different Epichloë-perennial ryegrass symbiota on the feeding behaviour of R. padi using the electrical penetration graph technique while also assessing the aphid life history. In most cases, endophytes had significant feeding deterrence and paired fecundity and mortality effects. But, in some instances, endophytes with the highest aphid mortality did not significantly deter feeding, suggesting a more complicated scenario of interactions between the relative concentration of metabolites, e.g., host plant defence response metabolites and alkaloids, and/or physical changes to leaf morphology. Overall, this study sheds light on the mode of action of Epichloë endophytes against aphids and highlights the importance of Epichloë-perennial ryegrass symbiota in the management of insect pests such as aphids in pasture-based grazing systems.

Stable introduction of Wolbachia wPip into invasive Anopheles stephensi for potential malaria control.

The spread and invasion of the urban malaria vector Anopheles stephensi has emerged as a significant threat to ongoing malaria control and elimination efforts, particularly in Africa. The successful use of the maternally inherited endosymbiotic bacterium Wolbachia for arbovirus control has inspired the exploration of similar strategies for managing malaria vectors, necessitating the establishment of a stable Wolbachia-Anopheles symbiosis. In this study, we successfully transferred Wolbachia wPip into An. stephensi, resulting in the establishment of a stable transinfected HP1 line with 100% maternal transmission efficiency. We demonstrate that wPip in the HP1 line induces nearly complete unidirectional cytoplasmic incompatibility (CI) and maintains high densities in both somatic and germline tissues. Despite a modest reduction in lifespan and female reproductive capacity, our results suggest the Wolbachia infection in the HP1 line has little impact on life history traits, body size, and male mating competitiveness, as well as the ability of its larvae to tolerate rearing temperatures up to 38°C, although wPip densities moderately decrease when larvae are exposed to a constant 33°C and diurnal cyclic temperatures of 27-36°C and 27-38°C. These findings highlight the potential of the HP1 line as a robust candidate for further development in malaria control.

Effects of Adverse Life History on Oxidative Stress and Cytokine Concentration in Domestic Dogs

ABSTRACT Early-life stress has been well studied in humans and laboratory animals; however, the impacts of similar adversity on the welfare of domestic dogs has recently begun to be addressed. For example, associations between processes linked to mitochondrial function, such as oxidative stress (OS) and proinflammatory immune systems, have been under-researched. Yet, mitochondria are targets and mediators of stress pathologies. This study investigates the impact of early-life stress on OS and proinflammatory immune responses in shelter dogs compared to client-owned dogs. We measured OS markers, including total antioxidant capacity (TAC), lipid oxidative damage, catalase (CAT) activity, glutathione peroxidase (GPx) activity, and superoxide dismutase (SOD) concentration, as well as inflammatory cytokines IL-1β, IL-6, and TNF-α. Shelter dogs exhibited significantly higher lipid oxidative damage (p = 0.0265), lower CAT activity (p = 0.002), higher SOD concentration (p < 0.001), and increased IL-1β levels (p = 0.027) compared to client-owned dogs. Compared to client-owned dogs, shelter dogs showed increased OS and inflammation, suggesting higher susceptibility to zoonotic and chronic diseases.

The development of ecological systems along paths of least resistance

A long-standing question in biology is whether there are common principles that characterize the development of ecological systems (the appearance of a group of taxa), regardless of organismal diversity and environmental context.1,2,3,4,5,6,7,8,9,10,11 Classic ecological theory holds that these systems develop following a sequenced, orderly process that generally proceeds from fast-growing to slow-growing taxa and depends on life-history trade-offs.2,12,13 However, it is also possible that this developmental order is simply the path with the least environmental resistance for survival of the component species and hence favored by probability alone. Here, we use theory and data to show that the order from fast- to slow-growing taxa is the most likely developmental path for diverse systems when local taxon interactions self-organize in light of environmental resistance. First, we demonstrate theoretically that a sequenced development is more likely than a simultaneous one, at least until the number of iterations becomes so large as to be ecologically implausible. We then show that greater diversity of taxa and life histories improves the likelihood of a sequenced order from fast- to slow-growing taxa. Using data from bacterial and metazoan systems,14,15,16,17,18,19 we present empirical evidence that the developmental order of ecological systems moves along the paths of least environmental resistance. The capacity of simple principles to explain the trend in the developmental order of diverse ecological systems paves the way to an enhanced understanding of collective features of life.

Life history of different giant water bug, Appasus japonicus (Belostomatidae: Heteroptera), populations throughout the Japanese Archipelago

AbstractAs temperatures in the Japanese Archipelago vary, species widely distributed in Japan should have different active periods, ecologies, and life histories. Information on developmental zero and effective accumulated temperature is necessary to estimate life history. The giant water bug, Appasus japonicus, found in rice paddies, ponds, and other lentic waters, is distributed throughout Japan except for the Ryukyu Islands, and is a representative taxon in which the male cares for the eggs. In this study, we investigated the life history, including the length of the breeding season, of different A. japonicus populations from Hokkaido, Ibaraki, Hyogo, and Nagasaki. The developmental period, developmental zero, and effective accumulated temperature of the eggs and nymphs showed no significant differences among the populations. The life history for each population was estimated based on climate data. This showed that the Hokkaido population had a shorter and more extremely limited breeding season than the other populations. The estimated number of generations during one breeding season was approximately one in Hokkaido, two in Ibaraki and Hyogo, and three in Nagasaki. The estimated number of times males cared for eggs during the breeding season was less than one in Hokkaido, eight in Ibaraki and Hyogo, and twelve in Nagasaki. Although Belostomatidae may carry multiple egg masses during the breeding season, this study shows that the male of the Hokkaido population does not care for multiple egg masses.

Daphnia stress response to environmental concentrations of chloramphenicol—multi-omics approach

Commonly used medicines, when discarded or improperly disposed of, are known to contaminate freshwater ecosystems. Pharmaceuticals can be toxic and mutagenic, and can modify freshwater organisms, even at environmentally relevant concentrations. Chloramphenicol (CAP) is an antibiotic banned in Europe. However, it is still found in surface waters around the world. The aim of this study was to evaluate the impact of chloramphenicol contamination in freshwater on the model organism Daphnia magna. Specific life history parameters, proteome, and host-associated microbiome of four D. magna clones were analyzed during a three-generation exposure to CAP at environmental concentrations (32 ng L−1). In the first generation, no statistically significant CAP effect at the individual level was detected. After three generations, exposed animals were smaller at first reproduction and on average produced fewer offspring. The differences in D. magna’s life history after CAP treatment were in accordance with proteome changes. D. magna’s response to CAP presence indicates the high stress that the tested organisms are under, e.g., male production, upregulation of ubiquitin-conjugating enzyme E2 and calcium-binding protein, and downregulation of glutathione transferase. The CAP-exposed D. magna proteome profile confirms that CAP, being reactive oxygen species (ROS)-inducing compounds, contributes to structural changes in mitochondria. Microbiome analysis showed a significant difference in the Shannon index between control and CAP-exposed animals, the latter having a more diverse microbiome. Multilevel analyses, together with long exposure in the laboratory imitating conditions in a polluted environment, allow us to obtain a more complete picture of the impact of CAP on D. magna.

Context-specific variation in life history traits and behavior of Aedes aegypti mosquitoes.

Aedes aegypti, the vector for dengue, chikungunya, yellow fever, and Zika, poses a growing global epidemiological risk. Despite extensive research on Ae. aegypti's life history traits and behavior, critical knowledge gaps persist, particularly in integrating these findings across varied experimental contexts. The plasticity of Ae. aegypti's traits throughout its life cycle allows dynamic responses to environmental changes, yet understanding these variations within heterogeneous study designs remains challenging. A critical aspect often overlooked is the impact of using lab-adapted lines of Ae. aegypti, which may have evolved under laboratory conditions, potentially altering their life history traits and behavioral responses compared to wild populations. Therefore, incorporating field-derived populations in experimental designs is essential to capture the natural variability and adaptability of Ae. aegypti. The relationship between larval growing conditions and adult traits and behavior is significantly influenced by the specific context in which mosquitoes are studied. Laboratory conditions may not replicate the ecological complexities faced by wild populations, leading to discrepancies in observed traits and behavior. These discrepancies highlight the need for ecologically relevant experimental conditions, allowing mosquito traits and behavior to reflect field distributions. One effective approach is semi-field studies involving field-collected mosquitoes housed for fewer generations in the lab under ecologically relevant conditions. This growing trend provides researchers with the desired control over experimental conditions while maintaining the genetic diversity of field populations. By focusing on variations in life history traits and behavioral plasticity within these varied contexts, this review highlights the intricate relationship between larval growing conditions and adult traits and behavior. It underscores the significance of transstadial effects and the necessity of adopting study designs and reporting practices that acknowledge plasticity in adult traits and behavior, considering variations due to larval rearing conditions. Embracing such approaches paves the way for a comprehensive understanding of contextual variations in mosquito life history traits and behavior. This integrated perspective enables the synthesis of research findings across laboratory, semi-field, and field-based investigations, which is crucial for devising targeted intervention strategies tailored to specific ecological contexts to combat the health threat posed by this formidable disease vector effectively.

Biotic and abiotic factors associated with genome size evolution in oaks.

The evolutionary processes that underlie variation in plant genome size have been much debated. Abiotic factors are thought to have played an important role, with negative and positive correlations between genome size and seasonal or stressful climatic conditions being reported in several systems. In turn, variation in genome size may influence plant traits which affect interactions with other organisms, such as herbivores. The mechanisms underlying evolutionary linkages between plant genome size and biotic and abiotic factors nonetheless remain poorly understod. To address this gap, we conducted phylogenetically controlled analyses testing for associations between genome size, climatic variables, plant traits (defenses and nutrients), and herbivory across 29 oak (Quercus) species. Genome size is significantly associated with both temperature and precipitation seasonality, whereby oak species growing in climates with lower and less variable temperatures but more variable rainfall had larger genomes. In addition, we found a negative association between genome size and leaf nutrient concentration (found to be the main predictor of herbivory), which in turn led to an indirect effect on herbivory. A follow-up test suggested that the association between genome size and leaf nutrients influencing herbivory was mediated by variation in plant growth, whereby species with larger genomes have slower growth rates, which in turn are correlated with lower nutrients. Collectively, these findings reveal novel associations between plant genome size and biotic and abiotic factors that may influence life history evolution and ecological dynamics in this widespread tree genus.

Voice of women's freedom: Women’s perspective through movie Before, Now &amp; Then (2022)

&lt;p&gt;&lt;em&gt;The movie Before, Now &amp;amp; Then (2022) raises the theme of Nana's life history as she breaks away from patriarchal hegemony through women's perspectives. Inspired by director Kamila Andini's anxiety about the role of women in movies, she invites the audience to experience the story from a woman's perspective. This study aims to analyze the voice of women's freedom from the female gaze through the movie Before, Now &amp;amp; Then (2022) using Baudrillrad's simulacra method and a postcolonial perspective. This approach seeks to uncover the colonialism practices behind the movie as a superstructure of power, providing a basis for analyzing the movie. The main female character is portrayed as someone who speaks little but takes decisive action to liberate herself from patriarchy by using women's language and point of view. The movie’s storyline focuses more on the life story of Nana to escape from the patriarchal restraints inherent in people's lives, especially since the movie's storyline was in the post-ideological war West Java in 1960. The study concludes that this movie can depict women’s perspectives through its characterization and storyline.&lt;br /&gt;&lt;br /&gt;&lt;/em&gt;&lt;em&gt;&lt;/em&gt;&lt;/p&gt;