The novelty of this research lies in its investigation of the initiation of thermal instability within a Darcy–Brinkman porous layer containing a Jeffrey nanofluid under the influence of rotation. While non-Newtonian fluids complex flow patterns and heat transfer enhancements due to thermal gradients are known, this study addresses a specific and previously unexplored aspect, the impact of rotation on Darcy–Brinkman porous layer containing a Jeffrey nanofluid. When a fluid is subjected to rotation, it can induce additional forces and flow patterns within the fluid. The linear stability theory and normal mode analysis method are used to analyze the stability of the system analytically and are computed numerically using the software Mathematica version 11.3. The investigation focuses on understanding the influence of various factors, namely, the Taylor number, the Lewis number, the Jeffrey parameter, the modified diffusivity ratio, nanoparticles Rayleigh number, the Darcy–Brinkman number, and medium porosity on the onset of thermal instability in the physical system. This novel perspective can contribute to a deeper understanding of heat transfer processes in non-Newtonian fluids and has potential applications in improving the efficiency of industrial systems, such as cooling systems, heat exchangers, and microfluidic devices where efficient heat transfer is crucial for performance improvement.