Purpose: This research is aimed to identify the mediating role of psychological safety and life satisfaction in the effect of organizational support on organizational trust from the healthcare workers' perspective. Methodology: Cross-sectional design was preferred in line with the purpose of the study. This cross-sectional study was carried out on all healthcare professionals of a private healthcare organization which operates as a branch of a chain hospital group in Ankara. A total of 450 people expressed their willingness to participate in the questionnaire. However, 53 people could not finish the survey due to overcrowding. Thus, the final sample comprised of 397 people. Data were collected by face-to-face survey method. Path analysis, one of the structural equation modeling (SEM) methods, was used in the analysis of the data set. Findings: This research finds that organizational support directly and indirectly affects organizational trust in a significant way and that organizational trust will rise as organizational support grows. When the mediating variables of psychological safety and life satisfaction are entered into the model together, the effect of organizational support on organizational trust is not significant. Thus, psychological safety and life satisfaction are full mediating variables. These results indicate that as the level of organizational support increases, the level of organizational trust can be indirectly increased through the mediation of psychological safety and life satisfaction. Originality: These findings suggest that organizational support directly and indirectly significantly impacts organizational trust and that psychological safety and life satisfaction mediate the association between organizational support and organizational trust.