Dissolved Oxygen Research Articles

Implementation of a novel bubble continuous positive airway pressure system with a blender in preterm infants in a low resource setting.

To determine if a novel CPAP system is associated with physiologic improvement in premature infants in a low resource setting and if the introduction of blended oxygen would reduce FiO2. Feasibility study of infants ≤2000 g or ≤32 weeks gestational age with early respiratory distress who were placed on Vayu CPAP with continuous pulse oximetry. Physiologic parameters were recorded prior to initiation and through the first 24 h. Seventy-six infants of birthweight 1360 ± 324 g and gestational age 31.2 ± 2.5 weeks were included. Compared to baseline, heart rate, respiratory rate, FiO2, and Silverman Anderson score significantly decreased while oxygen saturations significantly increased at one hour with persistence through 24 h. Utilization of Vayu CPAP in premature infants with respiratory distress was associated with immediate improvement in physiologic parameters. Use of blended oxygen coupled with pulse oximetry facilitates reduction in delivered oxygen.

High-resolution observations of the ocean upper layer south of Cape St. Vincent, the western northern margin of the Gulf of Cádiz

Abstract. This article presents an Eulerian physical and biogeochemical dataset from the Iberian Margin Cape St. Vincent Ocean observatory (IbMa-CSV), a facility of the European Multidisciplinary Seafloor and water column Observatory European Research Infrastructure Consortium (EMSO-ERIC), located 10 nautical miles south of Cape St. Vincent (Portugal), the southwest tip of the Iberian Peninsula and western limit of the northern margin of the Gulf of Cádiz (GoC). The observatory was installed on the shelf break, and the data time series spans 4 months for most of the variables. The upper 150 m were sampled intensively with a wave-powered vertical profiler at an average rate of 4.5 profiles per hour recording at 2 Hz when ascending at an approximate velocity of 0.2 m s−1 and 10 Hz when descending at a variable velocity. The vertical resolution was always higher than 0.2 m. Measured channels were conductivity, temperature, pressure, chlorophyll a, dissolved O2 concentration, and turbidity. Derived channels are sea pressure, depth, salinity, speed of sound, specific conductivity, dissolved O2 saturation, density anomaly, spiciness, and Brunt–Väisälä frequency. The acquired dataset includes the flow velocity and direction along the water column, taken from an upward-looking 300 kHz acoustic Doppler current profiler (ADCP) recorded every hour for 3 m depth bins extending the same depth range of the vertical profiler. A standard quality-control scheme was applied to the dataset. The dataset is preserved for multiple use and is accessible in the Sea Open Scientific Data Publication (SEANOE) repository via the following address: https://doi.org/10.17882/94769 (Rautenbach et al., 2022).

Prediction of the Flame Characteristics of an Industrial Gas Turbine Operated With CO2-Diluted Air Through a High-Fidelity Numerical Analysis: A Comparison Between Flamelet Generated Manifolds and Artificially Thickened Flame

Abstract In light of the global commitment to decarbonize industrial processes, carbon capture and storage (CCS) plays a pivotal role in mitigating greenhouse gas emissions from gas turbine (GT) power generation processes. Achieving an efficient GT–CCS coupling requires the employment of high percentages of exhaust gas recirculation (EGR) to maximize the CO2 content at the CCS inlet. Nevertheless, such operating conditions pose critical challenges for conventional combustion systems due to reduced oxygen levels associated with higher EGR, limiting engine operability. To address this challenge, the development of innovative technical solutions is essential to extend the combustor operational capabilities at high EGR rates. For this goal, a significant number of computational fluid dynamics (CFD) simulations are required to identify the flame stability limits across various EGR levels and burner designs. It is imperative, in this context, to minimize computational costs while maintaining high accuracy. In this work, a comprehensive comparative study of an extended version of the flamelet generated manifolds (FGM) and the artificially thickened flame (ATF) model is performed through a large eddy simulation (LES)-based CFD analysis. The investigation is performed within the context of an industrial lean-premixed burner manufactured by Baker Hughes, operating with natural gas and CO2-diluted air at atmospheric pressure. While the extended-FGM has been previously presented by the authors in a study on the same test rig under standard air conditions, the current work aims to extend its application to critical oxygen-depleted conditions, where near-blowout phenomena such as flame liftoff and length elongation may become significantly pronounced. Numerical validation is carried out through a direct comparison of the computed averaged heat release, representing the flame topology, with detailed OH* chemiluminescence images from a test campaign conducted by the technology for high temperature (THT) Lab of the University of Florence. The experimental data will serve as the primary benchmark for assessing the models’ effectiveness in capturing the main dynamics of such critical operating conditions. Furthermore, potential disparities in both thermal and flow fields at the burner exit region between the two models will be discussed.

Hypoxic Effects of Heroin and Fentanyl and Their Basic Physiological Mechanisms.

Respiratory depression that diminishes oxygen delivery to the brain is the most dangerous effect of opioid drugs. While plethysmography is a valuable tool to examine drug-induced changes in respiration, the primary cause of brain abnormalities induced by opioids is the global decrease in brain oxygen levels. The primary goal of this review is to provide an overview and discussion on fluctuations in brain oxygen levels induced by opioids, with a focus on heroin and fentanyl. To evaluate fluctuations in brain oxygen levels we used oxygen sensors coupled with high-speed amperometry in awake, freely moving rats. First, we provide an overview of brain oxygen responses induced by natural physiological stimuli and discuss the mechanisms regulating oxygen entry into brain tissue. Then, we present data on brain oxygen responses induced by heroin and fentanyl and review their underlying mechanisms. These data allowed us to compare the effects of these drugs on brain oxygen regarding their latency, potency, time-dependency, and potential lethality at high doses as well as their relationships with peripheral oxygen responses. We also discuss data on the effects of naloxone on brain oxygen responses induced by heroin and fentanyl in the paradigms of both the pre-treatment and treatment, when naloxone is administered at different times after the primary opioid drug. Although most data discussed were obtained in rats, they may have clinical relevance for understanding the mechanisms underlying the physiological effects of opioids and developing rational treatment strategies to decrease acute lethality and long-term health complications of opioid misuse.

Effects of Teifairia occidentalis and Vernonia amygdalina Supplements on Growth Performance of Clarias gariepinus Fingerlings

This study examined the growth-enhancing potential of Telfaira occidentalis and Vernonia amygdalina leaf powder in Clarias gariepinus culture. The experiment was conducted at Tiddo Fish Farm Makurdi. One thousand juvenile catfish were obtained from the farm’s hatchery and allocated to five treatment groups identified as Control (0% supplement inclusion), T1 (10% fluted pumpkin leaf powder inclusion), T2 (20% fluted pumpkin leaf powder inclusion), T3 (10% bitter leaf powder inclusion) and T4 (20% bitter leaf powder inclusion). The fish were fed thrice daily for 8 weeks and water changed daily, to determine growth parameters of fish and physico-chemical parameters of water. Results obtained show that after the treatment period, Mean Weight gain and Specific Growth Rate was not significantly different (p>0.05). However, Specific Growth Rate and Percentage Survival rate differed significantly (p<0.05), highest (7.9% and 100%) in T3 respectively. Water quality parameters such as pH, Dissolve Oxygen and Temperature were within the recommended range and not significantly affected. Both plant species feed inclusions were utilized by fish, which was observed to facilitate the growth and survival of the fish species, with high percentage survival. In conclusion, T3 inclusion levels showed better growth performance.

Safety and tolerability of a Muse cell-based product in neonatal hypoxic-ischemic encephalopathy with therapeutic hypothermia (SHIELD trial).

Hypoxic-ischemic encephalopathy (HIE), associated with high mortality and neurological sequelae, lacks established treatment except therapeutic hypothermia. Clinical-grade multilineage-differentiating stress-enduring (Muse) cells (CL2020) demonstrated safety and efficacy in nonclinical HIE rat models, thereby leading to an investigator-initiated clinical trial to evaluate CL2020 safety and tolerability in neonatal HIE as a single-center open-label dose-escalation study with 9 neonates with moderate-to-severe HIE who received therapeutic hypothermia. Each patient received a single intravenous injection of CL2020 cells between 5 and 14 days of age. The low-dose (3 patients) and high-dose (6 patients) groups received 1.5 × 106 and 1.5 × 107 cells/dose, respectively. The occurrence of any adverse event within 12 weeks following CL2020 administration was the primary endpoint of this trial. No significant changes in physiological signs including heart rate, blood pressure, and oxygen saturation were observed during or after administration. The only adverse event that may be related to cell administration was a mild γ-glutamyltransferase level elevation in one neonate, which spontaneously resolved without any treatment. All patients enrolled in the trial survived, and normal developmental quotients (≥ 85) in all 3 domains of the Kyoto Scale of Psychological Development 2001 were observed in 67% of the patients in this trial. CL2020 administration was demonstrated to be safe and tolerable for neonates with HIE. Considering the small number of patients, a randomized controlled confirmatory study is warranted to verify these preliminary findings and evaluate the efficacy of this therapy.

Effect of hypoxia in the post-hatching development of the salmon (Salmo salar L.) spinal cord

IntroductionHypoxia has a teratogenic effect on the fish during embryonic development. Nevertheless, the effects on the larval stage are not yet known. Therefore, the aim of this study was to assess the effects of hypoxia on the number of neurons and their apoptotic rate in the spinal cord of Salmo salar alevins after hatching.MethodsWe used a total of 400 alevins, establishing both hypoxia and control (normoxia) groups (n = 8), considering post-hatching days 1, 3, 5, and 7, each with 50 individuals. Transversal sections of 50 μm thickness were cut from the alevin body. We performed cresyl-violet staining and counted the spinal cord neurons. Also, immunohistochemistry for HIF-1α and caspase-3 were used. For statistical analysis ANOVA one-way and Tukey's Test were used.ResultsHIF-1α was expressed in spinal neurons in both the hypoxic and normoxic groups, with the former being significantly higher. Both the hypoxic and normoxic groups evidenced the process of neuronal apoptosis, with the hypoxic groups demonstrating a higher significance. The number of neurons in the spinal cord was significantly lower in the hypoxic group.DiscussionWe found that when oxygen levels in the aquatic environment were low in Salmo salar farming alevins post-hatch, the number of spinal neurons dropped by half. These results contribute to increasing our knowledge of the biological development of salmon, in particular the genesis of the spinal cord, and the effects of hypoxic conditions on the development of this structure of the nervous system.

Photobiomodulation therapy on puncture-associated pain: A controlled randomized double-blind clinical trial.

Dental fear and phobia are prevalent worldwide, with local anesthesia being the most feared procedure. This study aimed to determine whether photobiomodulation therapy (PBMT), used as a pre-anesthetic, could modulate puncture pain and enhance the effectiveness of local anesthesia. In this controlled, randomized, double-blind study, 49 participants were divided into an experimental group (n = 24), which received infrared laser therapy (100 mW, at 808 nm, 8 J, 80 s at a single point) immediately before standard anesthesia; and control group (n = 25), which received the standard anesthetic technique and sham laser. Pain levels were measured using the visual analog scale, and anesthetic efficacy was assessed through electrical tests (latency), percentage of failures, and cartridge usage. Anxiety levels were evaluated using the Beck Anxiety Inventory. Cardiovascular parameters were evaluated through blood pressure, oxygen levels, and heart rate. This randomized, double-blind study found no difference between groups in these experimental conditions. The bias toward a positive PBMT result was sufficiently removed. Autonomic responses of the PBMT group were maintained stable during the procedure.

Intermittent sigh breaths during high-frequency oscillatory ventilation in preterm infants: a randomised crossover study

ObjectiveTo determine if combining high-frequency oscillatory ventilation (HFOV) with additional sigh breaths would improve end-expiratory lung volume (EELV) and oxygenation in preterm infants.DesignProspective interventional crossover study.SettingNeonatal intensive care unit.PatientsVentilated preterm...

COVID-19 vaccination and lethality reduction: a prospective observational study in Venezuela during the last two waves

BackgroundIn Venezuela, the predominant vaccines administered are BBIBP-CorV and Gam-COVID-Vac. Despite robust evidence from randomized clinical trials validating the effectiveness of COVID-19 vaccines in mitigating hospitalization and mortality, there is still a lack of post-authorization safety studies conducted within this demographic population.MethodsA prospective observational study from October 5, 2021 to March 31, 2022 encompassed COVID-19 vaccinated and unvaccinated patients from four sentinel hospitals in Venezuela. Patient lethality was predicted using Charlson Comorbidity index. Clinical outcomes were assessed through WHO’s COVID-19 Clinical Progression Scale.ResultsOut of the 175 patients assessed, 85 (48.6%) were vaccinated. The median Charlson Comorbidity index was 3 points, with no statistically significant differences observed between the groups (p = 0.2). A total of 50 (28.6%) patients died during the study period, with higher proportion of deaths in unvaccinated patients (35.6% vs. 21.2%, p = 0.035). Advanced age (OR = 1.043, 95% CI = 1.015–1.071, p = 0.002) was associated with increased death risk, whereas vaccination against COVID-19 (OR = 0.428, 95% CI = 0.185–0.99, p = 0.047), high oxygen saturation (OR = 0.964, 95% CI = 0.934–0.995, p = 0.024), and enoxaparin administration (OR = 0.292, 95% CI = 0.093–0.917, p = 0.035) were associated with decreased death risk.ConclusionDuring the third and fourth waves of the pandemic, COVID-19 vaccination was associated with a 57% reduction in lethality among patients in four public hospitals in Venezuela.

Hyperspectral imaging of human liver allografts for prediction of initial graft function

PurposeIschemia reperfusion injury represents a significant yet difficult to assess risk factor for short- and long-term graft impairment in human liver transplantation (LT). As a non-invasive, non-ionizing tool, hyperspectral imaging (HSI) is capable of correlating optical properties with organ microperfusion. Hence, we here performed a study of human liver allografts assessed by HSI for microperfusion and prediction of initial graft function.MethodsImages of liver parenchyma of 37 human liver allografts were acquired at bench preparation, during normothermic machine perfusion (NMP), if applicable, and after reperfusion in the recipient. A specialized HSI acquisition software computed oxygen saturation (StO2), tissue hemoglobin indices (THI), near infrared perfusion indices (NIR), and tissue water indices (TWI). HSI parameters were analyzed for differences with regard to preservation technique, reperfusion sequence and presence of early allograft dysfunction (EAD).ResultsOrgan preservation was performed by means of NMP (n = 31) or static cold storage (SCS; n = 6). Patients’ demographics, donor characteristics, presence of EAD (NMP 36.7% vs. SCS 50%, p = 0.6582), and HSI parameters were comparable between both groups of preservation method. In organs developing EAD, NIR at 1, 2, and 4 h NMP and after reperfusion in the recipient was significantly lower (1 h NMP: 18.6 [8.6–27.6] vs. 28.3 [22.5–39.4], p = 0.0468; 2 h NMP: 19.4 [8.7–30.4] vs. 37.1 [27.5–44.6], p = 0.0011; 4 h NMP: 26.0 [6.8–37.1] vs. 40.3 [32.3–49.9], p = 0.0080; reperfusion: 13.0 [11.5–34.3] vs. 30.6 [19.3–44.0], p = 0.0212).ConclusionHSI assessment of human liver allografts is feasible during organ preservation and in the recipient. NIR during NMP and after reperfusion might predict the onset of EAD. Larger trials are warranted for assessment of this novel technique in human LT.

Kaempferol Reduces Cardiopulmonary Load and Muscular Damage in Repeated 400‐m Sprints: A Double‐Blind, Randomized, Placebo‐Controlled Trial

ABSTRACTPlants exposed to hypoxic conditions have been suggested to produce more biologically active phytochemicals than those exposed to normal oxygen levels. Previously, we investigated 314 highland crop species and showed that the flavonoid kaempferol extracted from a highland quinoa grain markedly increased mitochondrial metabolism and ATP production in a hypoxic environment in vitro. Thus, we hypothesized that kaempferol would be effective during exercise under harsh conditions, in which anaerobic metabolism occurs. This study adopted a double‐blind, placebo‐controlled, crossover design to investigate the effect of a single oral dose of kaempferol (10 mg) on the athletic performance‐related indicators of 13 male university athletes (20.8 ± 0.7 years) who performed two consecutive 400‐m runs with the shortest 90‐min interval assuming qualifying and main races. Although no significant differences were observed in the 400‐m race times between the placebo and kaempferol groups, kaempferol intake markedly reduced the respiratory and heart rates during the second run (p < 0.05). In addition, kaempferol intake reduced the levels of muscle damage markers, myoglobin, and aspartate transaminase (p < 0.05). A single oral dose of kaempferol reduced the cardiopulmonary burden and muscle damage in individuals participating in 400‐m runs. Kaempferol may be a useful supplement for relieving the physical load, particularly in individuals performing strenuous exercises with high oxygen demand.Trial Registration: UMIN Clinical Trials Registry in Japan: UMIN000049588

Understanding Diabetic Wounds: A Review of Mechanisms, Pathophysiology, and Multimodal Management Strategies

Introduction: Diabetic wounds are a prevalent and impairing consequence of diabetes mellitus that significantly impacts people's lives and global healthcare systems. Because of disturbances in the wound-healing cascade, these intricate, persistent wounds frequently refuse to heal. Focusing on diabetic wound formation, this study seeks to clarify the complex mechanisms and pathophysiology involved while offering a thorough overview of modern multimodal therapy techniques. The etiology of diabetic wounds entails a complex interplay between tissue destruction caused by hyperglycemia, neuropathy, ischemia, and compromised immune response. Extended inflammation, abnormal protease activity, and low oxygen levels in the tissue exacerbate the healing process. Comprehending these pathogenic mechanisms is essential for formulating efficacious therapeutic strategies. Methodology: A thorough evaluation of the literature was done. Databases like SciFinder, ScienceDirect, PubMed, Google, Google Scholar, and the Egyptian Knowledge Bank were used to find pertinent publications. More than 200 articles and databases were studied to constitute this paper. The accuracy of the retrieved data was carefully reviewed and cross-checked. The current review aims to define wounds, various methods of classification, and various advancements for wound management as mentioned in scheme 1. Several multidisciplinary strategies, including debridement, unloading, antimicrobial stewardship, and innovative therapeutics, are currently needed to manage diabetic wounds. Debridement—the excision of non-viable tissue—is necessary to create an environment that is conducive to recovery. Biomechanical interventions and offloading help to prevent additional tissue damage caused by repetitive stress. Antimicrobial treatments fight infections, which are a common diabetic wound consequence. Promising supplementary treatments are provided by developments in cellular and tissue-based products, ozone therapy growth factors, bioengineered skin substitutes, and hyperbaric oxygen therapy. Result: After applying article selection criteria and reviewing the quality of the methodology a total of 200 articles were selected to be included in the review. In this review, intricate interactions between peripheral neuropathy, vascular insufficiency, and hyperglycemia in the pathophysiology of diabetic wounds are explained. The efficacy of multimodal therapies is discussed in detail. Discussion: A thorough comprehension of the complex mechanisms that underlie diabetic wounds is essential for efficient therapy. This review emphasizes how important multimodal approaches are to treating the complex pathophysiology of these wounds. Clinicians can greatly enhance the prognosis of patients with diabetic foot ulcers by addressing vascular insufficiency, neuropathy, infection, and poor healing. Conclusion: Timely wound resolution remains a key difficulty despite the implementation of multimodal methods. To customize therapies, personalized medicine strategies utilizing genetic and proteomic biomarkers must be the main focus of future research. Furthermore, cutting-edge biotechnologies with the potential to transform diabetic wound treatment include optogenetics and nanomedicine.

Prediction of urban surface water quality scenarios using hybrid stacking ensembles machine learning model in Howrah Municipal Corporation, West Bengal

In the pursuit of understanding surface water quality for sustainable urban management, we created a machine learning modeling framework that utilized Random Forest (RF), Cubist, Extreme Gradient Boosting (XGB), Multivariate Adaptive Regression Splines (MARS), Gradient Boosting Machine (GBM), Support Vector Machine (SVM), and their hybrid stacking ensemble RF (SE-RF), as well as stacking Cubist (SE-Cubist), to predict the distribution of water quality in the Howrah Municipal Corporation (HMC) area in West Bengal, India. Additionally, we employed the ReliefF and Shapley Additive exPlanations (SHAP) methods to elucidate the underlying factors driving water quality. We first estimated the water quality index (WQI) to model seven water quality parameters: total hardness (TH), pH, total dissolved solids (TDS), dissolved oxygen (DO), biochemical oxygen demand (BOD), calcium (Ca), magnesium (Mg). Then six independent factors were utilized (i.e. Precipitation (Pr), Maximum Temperature (Tmax), Minimum Temperature (Tmin), Normalized Difference Turbidity Index (NDTI), Normalized Difference Chlorophyll Index (NDCI), and Total Dissolved Solids (TDS)) for predicting the WQI mapping through the different ML models. This study demonstrated that the SE-Cubist model outperforms other ML models. During the testing phase, it achieved the best modeling results with an R2 = 0.975, RMSE = 0.351, and MAE = 0.197. The ReliefF and SHAP analyses identified Pr and Tmax as the most significant factors influencing WQI within the study area.

Seasonal Distribution of Key Small-Sized Fish in the South Inshore of Zhejiang, China

Small-sized fish are a vital food source for large predatory commercial fish and play a key role in marine food webs, bridging lower and higher trophic levels. They are indispensable in maintaining the energy flow and material cycling within aquatic ecosystems. This study utilized bottom-trawl survey data from 2017 to 2020 along the south inshore of Zhejiang, China, complemented by concurrent environmental data, to examine the influence of environmental factors on the resource density and seasonal distribution patterns of four dominant small-sized fish species. The research findings indicated that SSH (sea surface height) and Chl (chlorophyll-a concentration) emerged as the key environmental factors influencing resource densities, with all four species exhibiting similar preferences toward these variables. However, significant disparities were observed in their preferences for SST (sea surface temperature), SSS (sea surface salinity), and DO (dissolved oxygen). The various species’ resource density and distribution patterns underwent significant seasonal variations. Additionally, the seasons and regions with the highest resource densities consistently aligned, occurring predominantly in autumn within the northern waters of the study area. This research further elucidated the environmental predilections and seasonal spatial distribution traits of small-sized fish in the south inshore of Zhejiang, an important feeding ground for economic fish species in the East China Sea. This provides scientific backing for forecasting alterations in coastal fishery resources under environmental and climate change scenarios and supports ecosystem-based fisheries management strategies.

Guideline-recommended basic parameter adherence in neurocritical care stroke patients: Observational multicenter individual participant data analysis.

Neurocritical care patients with neurovascular disease often face poor long-term outcomes, highlighting the pivotal role of evidence-based interventions. Although International Guidelines emphasize managing basic physiological parameters like temperature, blood glucose, blood pressure, and oxygen levels, physician adherence to these targets remains uncertain. This study aimed to assess adherence to guideline-based treatment targets for basic physiological parameters in neurocritical care. This multicenter observational study was conducted across eight tertiary University Hospitals in Germany analyzed 474 patients requiring mechanical ventilation (between January 1st and December 31st, 2021). Adherence was defined as the rate of measurements within therapeutic ranges for systolic blood pressure (situation-adapted), mean blood pressure (MAP, 60-90 mmHg), glucose levels (80-180 mg/dl), body temperature (<37.5°C), partial arterial pressure of oxygen (PaO2) 80-120 mmHg und partial arterial pressure of carbon dioxide (PaCO2) 35-45 mmHg during the initial 96 h of hospitalization in 4 hour-intervals. Overall, 70.7% of all measurements were within the predetermined therapeutic ranges including SBP (71.3%), temperature (68.3%), MAP (71.4%), PaO2 (65.2%), PaCO2 (75.0%) and blood glucose (80.7%). This multicenter study demonstrates adherence to guideline-based treatment targets, underscoring the high standards maintained by neurological intensive care units. Our study offers valuable insights into adherence to guideline-based treatment targets for neurocritical care patients in Germany. To improve patient care and optimize therapeutic strategies in neurovascular diseases, further research is needed to examine the impact of these adherence parameters on long-term outcomes.

The oxidative stress mechanism of Bacillus cereus spores induced by atmospheric pressure plasma jet

Emerging plasma technology has demonstrated its effectiveness in inactivating bacteria and spores, showing wide applicability in environment cleaning and in the food supply chain. Studies have revealed that the main reason of plasma-mediated bacterial inactivation were contributed to the oxidative damage on inner membranes (IMs) of cell, while few research paid attention to the oxidation stress process of lipid in spores’ IMs. This study investigated the impact of atmospheric pressure plasma jet (APPJ) treatment on spore inactivation, focusing on oxidative stress mechanisms and changes in membrane lipid composition. Results demonstrated that APPJ treatment induced significant oxidative stress within spores, characterized by elevated levels of reactive oxygen and nitrogen species (RONS). This oxidative stress led to the peroxidation of glycerophospholipids, particularly phosphatidylglycerol, phosphatidylcholine, and cardiolipin, major components of spore membranes. Subsequently, the rearrangement of membrane lipid and the formation of lipid hydroperoxide, caused by the released cations and RONS, accelerated membrane instability. These changes correlated with increased membrane permeability and structural damage observed via flow cytometry, indicating the critical role of membrane lipid peroxidation in spore inactivation. Furthermore, the study highlighted the potential of APPJ as a robust technology for enhancing food safety by targeting spore-forming pathogens throughout the food supply chain.

Protective effects of PDGF-AB/BB against cellular senescence in human intervertebral disc.

Cellular senescence, characterized by a permanent state of cell cycle arrest and a secretory phenotype contributing to inflammation and tissue deterioration, has emerged as a target for age-related interventions. Accumulation of senescent cells is closely linked with intervertebral disc (IVD) degeneration, a prevalent age-dependent chronic disorder causing low back pain. Previous studies have highlighted that platelet-derived growth factor (PDGF) mitigated IVD degeneration through anti-apoptosis, anti-inflammation, and pro-anabolism. However, its impact on IVD cell senescence remains elusive. In this study, human NP and AF cells derived from aged, degenerated IVDs were treated with recombinant human (rh) PDGF-AB/BB for 5 days and changes of transcriptome profiling were examined through mRNA sequencing. NP and AF cells demonstrated similar but distinct responses to the treatment. However, the effects of PDGF-AB and BB on human IVD cells were comparable. Specifically, PDGF-AB/BB treatment resulted in downregulation of gene clusters related to neurogenesis and response to mechanical stimulus in AF cells while the downregulated genes in NP cells were mainly associated with metabolic pathways. In both NP and AF cells, PDGF-AB and BB treatment upregulated the expression of genes involved in cell cycle regulation, mesenchymal cell differentiation, and response to reduced oxygen levels, while downregulating the expression of genes related to senescence associated phenotype, including oxidative stress, reactive oxygen species (ROS), and mitochondria dysfunction. Network analysis revealed that PDGFRA and IL6 were the top hub genes in treated NP cells. Furthermore, in irradiation-induced senescent NP cells, PDGFRA gene expression was significantly reduced compared to non-irradiated cells. However, rhPDGF-AB/BB treatment increased PDGFRA expression and mitigated the senescence progression through increased cell population in the S phase, reduced SA-β-Gal activity, and decreased expression of senescence related regulators including P21, P16, IL6, and NF-κB. Our findings reveal a novel anti-senescence role of PDGF in the IVD, demonstrating its ability to alleviate the senescent phenotype and protect against the progression of senescence. This makes it a promising candidate for preventing or treating IVD degeneration by targeting cellular senescence.

An algorithmic approach to arterial blood gas analysis for cardiologists

Abstract: An arterial blood gas (ABG) is a laboratory test to monitor the patient’s acid base balance. The body regulates pH closely to maintain homeostasis. This test is used to determine the magnitude of the compensation by the buffer system and includes the measurements of the acidity (pH), levels of oxygen, and carbon dioxide in arterial blood. Acid base disorders can lead to severe complications in many disease states. ABG interpretation is paramount in critically ill patients. Many methods already exist to guide the interpretation of the ABG. This article does not include methods such as analysis of base excess or Stewart’s strong ion difference. It is paramount for cardiologists to understand ABG interpretation to manage their patients effectively. Interpreting ABGs may seem complex, but a systematic approach is required to diagnose and treat disorders by quick and easy ABG interpretation.

Retinal oxygen metabolic function in choroideremia and retinitis pigmentosa.

To measure the retinal oxygen metabolic function with retinal oximetry (RO) in patients with choroideremia (CHM) and compare these findings with retinitis pigmentosa (RP) patients and controls. Prospective observational study including 18 eyes of 9 molecularly confirmed CHM patients (9♂; 40.2 ± 21.2years (mean ± SD), 77 eyes from 39 patients with RP (15♀ 24♂; 45.6 ± 14.7years) and 100 eyes from 53 controls (31♀ 22♂; 40.2 ± 13.4years). Main outcome parameters were the mean arterial (A-SO2; %), venular (V-SO2; %) oxygen saturation, and their difference (A-V SO2; %) recorded with the oxygen saturation tool of the Retinal Vessel Analyzer (IMEDOS Systems UG, Germany). Statistical analyses were performed with linear mixed-effects models. Eyes suffering from CHM differed significantly from both RP and control eyes, when the retinal oxygen metabolic parameters were taken into account. While RP showed significantly higher A-SO2 and V-SO2 values when compared to controls, CHM showed opposite findings with significantly lower values when compared to both RP and controls (P < 0.001). The A-V SO2, which represents the retinal oxygen metabolic consumption, showed significantly lower values in CHM compared to controls. The retina in CHM is a relatively hypoxic environment. The decrease in oxygen levels may be due to the profound choroidal degeneration, leading to decreased oxygen flux to the retina. RO measurements may help understand the pathogenesis of CHM and RP. These findings may provide useful details to inform the planning of clinical trials of emerging therapies for CHM. What was known before? Retinal oxygen metabolic function measured with retinal oximetry (RO) shows significant alterations in patients with retinitis pigmentosa. RO function in choroideremia is significantly altered when compared to controls. Furthermore, RO in choroideremia shows opposing findings within different oxygen metabolic parameters to those that were so far known for retinitis pigmentosa. By providing insights into the retinal oxygen metabolic mechanisms, RO can help understand the underlying pathophysiology in choroideremia.