Plants were regenerated in vitro from intact lamina of Rubus laciniatus Willd. `Thornless Evergreen' (TE), a chimeral thornless form of `Evergreen' blackberry, which carries a thornless gene only in its epidermal tissue. Leaves without petioles or axillary buds were harvested from proliferating cultures and explanted onto modified Murashige and Skoog (MS) medium with various levels of BA, NAA and thidiazuron (TDZ). TDZ gave more consistent regeneration than BA. Up to 100% regeneration was observed with 0.2 μM TDZ plus NAA (0.25 or 1.0 μM) or 0.1 μM TDZ plus 1.0 μM NAA.Regenerants from chimeral TE and a pure thornless somaclone (IL 6-6), were classified according to stem and petiole morphology and presence or absence of prickles. Most (74%) of the regenerants from chimeral TE showed marked stem and petiole twisting compared to 4% of the regenerants from IL 6-6. The plants with straightest petioles tended to have the most prickles. These regenerants have been field planted. Additional observations of these regenerants will be discussed.