Abstract
Shoot regeneration was obtained from leaf tissues of American (Ulmus americana) and Siberian elm (U. pumila) seedlings germinated in vitro and in greenhouse. Murashige and Skoog (MS, 1962) media supplemented with 4 levels of BA (0, 5, 10, 15, 20 μM) and 3 levels of IBA (0, 2.5, 5.0 μM) were tested in a factorial design to find an optimal hormonal combination for shoot regeneration. Shoot regeneration was obtained from both species within 3-4 weeks in a wide range of media. The highest regeneration rate (50%) of American elm was in the medium containing 10 μM BA and 2.5 μM IBA. Incubation under the light was essential for a higher rate of regeneration. Gelrite was found as a better solidifying agent than agar. The progress is under way to achieve transgenic elms by combining this regeneration system with Agrobacterium-mediated transformation.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
