Root Level Research Articles

Socio-Managerial Framework of Health Governance: Empirical Evidence from India’s National Sanitation Program (SBM-G)

Given that citizen participation is considered the main pillar of ‘Development’, the political economy behind its practice (behaviour and utilisation) remains a question. To disentangle the complex web of relationships that the governance shares with the citizens’ interface, it would be worthwhile to examine the whole phenomenon at the grass root level. A review of issues surrounding democracy has led different schools of thought to realise the need for adopting a holistic development approach for ensuring citizens’ participation in development processes. One such school believes that it is only through addressing governance bottlenecks and ensuring spaces for participation in policy design, programme formulation and implementation supplemented with proper monitoring, that ‘real’ development can be achieved. It is also universally accepted that governance is an enabler for socio-economic transformation and this can help in the improvement of lives through the eradication of structural inequality. Hence, strengthening the local governments is critical for ensuring citizen empowerment, civic participation and better service delivery. Furthermore, governance is measurable and can be monitored; thus can ensure a measurable implementation, accountability and monitoring framework (Global Thematic Consultation on Governance and the Post-2015 Development Framework, 2013). Against these backdrops, the current study endeavours to unearth plausible factors influencing the health behaviour of rural people examining a case of India’s National Sanitation Program—Swachh Bharat Mission in Odisha villages. Analysis of primary data collected from six districts across different regions of the Odisha state shows that various managerial, governance and social factors have a significant effect on the health behaviour of people and present more insightful results.

A micro–computed tomographic analysis of the root canal systems in the permanent mandibular incisors in a Chinese population

BackgroundComprehensive understanding of the root canal system complexity is critical important for successful root canal therapy. A double root canal system may be present in permanent mandibular incisors with a variable incidence in different ethnic populations. Ignorance or improper management of this canal variation can lead to treatment failure. This in vitro study aimed to identify the anatomic features of root canal systems in the mandibular incisors in a Chinese population by using micro-CT.MethodsA total of 106 permanent mandibular incisors (53 central incisors and 53 lateral incisors) were collected from a native Chinese population. The teeth were scanned by a micro-CT scanner and then reconstructed three-dimensionally. The canal configurations were detected by Vertucci’s classification, and the number and location of the accessory canals were also identified. The long (D) and short diameters (d) of the main and accessory canals were measured and D/d ratio was calculated at different root levels (cemento-enamel junction [CEJ] level, mid-root level and 1, 2, 3 and 4 mm from the apex). The root canal curvatures in the double-canaled mandibular incisors were measured at the proximal view by using modified Schneider’s method. Chi-square test or Fisher's exact test was used for comparison of occurrence rates. Comparison of means from multiple groups was performed by using one-way ANOVA and LSD post-hoc test.ResultsIn regard to the occurrence of double root canals, gender difference was neither detected in the mandibular central (16.0% [male] vs 14.3% [female]; p = 0.862), nor in the mandibular lateral incisors (26.9% [male] vs 33.3% [female]; p = 0.611). Age group difference was also not detected in the mandibular central (p = 0.717) and lateral incisors (p = 0.521). The incidence of double root canals was 15.1% (8/53) in the central incisors, and 30.2% (16/53) in the lateral incisors, but the difference did not reach statistical significance (p = 0.063). The most frequent non-single canal type was the type III (1–2-1) (18.9% [20/106]), and the other types identified included 1 case of type II (2–1) and 3 cases of type V (1–2). The incidence of accessory canals was 17.9% (19/106), with a mean level of 1.92 ± 1.19 mm from the apex. The frequency of long-oval (2 ≤ D/d < 4) and flattened canals (D/d ≥ 4), as well as the mean value of D, d and D/d ratio increased from the apical 1 mm to the apical 4 mm level (the D/d ratio increased from 1.9 to 2.9 for the single canals, from 1.4 to 3.3 for the buccal canals and from 1.2 to 2.3 for the lingual canals), and the D/d ratio reached the peak at the mid-root level. Double curvatures were detected in 33.3% (8/24) of the buccal canals and 37.5% (9/24) of the lingual canals, and the difference has no statistical significance (p = 0.063). The degrees of the primary curvatures were 21.5 ± 7.1 degrees for the buccal and 30.1 ± 9.2 degrees for the lingual canals, and the degrees of secondary curvatures were 27.0 ± 11.4 degrees for the buccal and 30.5 ± 12.5 degrees for the lingual canals in the double curvatures. The degrees of the single curvatures were 14.2 ± 6.3 degrees for the buccal and 15.6 ± 6.0 degrees for the lingual canals. Significant difference was detected among above 6 groups of canal curvatures (p = 0.000), and severe curvatures (≥ 20 degrees) were more frequently detected in the double curved canals.ConclusionsDouble-canaled mandibular incisors were not uncommon in the Chinese population, and type 1–2-1 was the most frequent non-single canal type. Gender and age did not significantly impact the occurrence of a second canal in mandibular incisors. Long-oval and flattened canals were very common at different root levels and their incidence increased from apex to the mid-root level. Severe curvatures were frequently detected in the double canal systems, especially in those canals with double curvatures.

Reduction in prevalence of spousal physical violence against women in India: evidence from three national surveys

Spousal physical violence (PV) against women is considered to be major health issue in developing countries. Lifetime physical violence is a composite outcome consists of hit, kick, beat, slap and threatened with weapon, perpetrated by the husband. The study aims to examine changes in prevalence and specific risk factors for PV from 1998 to 2016 in India. This study analyzed data from a cross sectional epidemiological survey in 1998–1999, NFHS-3 (2005–2006) and NFHS-4 (2015–2016) data. There was a significant decline of about 10% (95% CI: 8.8%–11.1%) in PV. Major risk factors for change in PV were husband’s use of alcohol, illiteracy and socio-economic status of the household. The Protection of Women from Domestic Violence Act may have played a role in reducing the PV. Even though there was a decline in PV, actions have to be implemented from the root level to ensure women empowerment.

Understanding Social Accountability at Rural Local Governance in Bangladesh and the Way Forward: In the Case of Union Parishad

In the 21st Century social accountability has become one of the preconditions to create a more transparent and representative government and aid public institutions in meeting the expectations of the population through empowering citizens which is necessary for effective and sustainable development. Social accountability of the elected representatives at the rural level may help the government increase service quality, empower rural citizens, and promote good governance. At present Bangladesh, mostly known as the “Development Surprise”, has leaped towards lower middle-income country status from a poor developing one within four decades of its decisive victory. In this stage of growth and development, the persistence of social accountability at the rural local government would ensure the attainment of sustainable development through ensuring demand-based service delivery. Against this backdrop, this paper intends to explore the present status of social accountability mechanisms at the Union Parishad which is the lowest tier of rural local governance in Bangladesh. Based on the analysis, several recommendations for the way forward have been summarized. Secondary data analysis and empirical knowledge have been exerted which would refer to a clear view of the current status of social accountability at the grass root level. The first section mentions the importance of social accountability at the union level based on theoretical analysis. Existing mechanisms to ensure social accountability at Union Parishad have been delineated in the second section. The third section develops a compact understanding of the current status of social accountability at the Union Parishad level. The fourth section will share a discussion of the findings, recommendations, and conclusion. This study finds that the lack of interest among Union Parishad Functionaries, avoidance by citizens, irregularities in committee formation, irregular meetings, lack of resources, clientelism and Govt. supervision, etc. have made the social accountability mechanisms at the rural local government ineffective and fruitless. The political willingness of the Government, GO-NGO collaboration, allocation of resources to rural local government, awareness raising among citizens, educating citizens politically, and ensuring proper training for the officials in the Union Parishad can play an important role to revive social accountability through deliberative forums and committees in the rural local government.

Young Tomato Plants Respond Differently under Single or Combined Mild Nitrogen and Water Deficit: An Insight into Morphophysiological Responses and Primary Metabolism.

This study aimed to understand the morphophysiological responses and primary metabolism of tomato seedlings subjected to mild levels of nitrogen and/or water deficit (50% N and/or 50% W). After 16 days of exposure, plants grown under the combined deficit showed similar behavior to the one found upon exposure to single N deficit. Both N deficit treatments resulted in a significantly lower dry weight, leaf area, chlorophyll content, and N accumulation but in a higher N use efficiency when compared to control (CTR) plants. Moreover, concerning plant metabolism, at the shoot level, these two treatments also responded in a similar way, inducing higher C/N ratio, nitrate reductase (NR) and glutamine synthetase (GS) activity, expression of RuBisCO encoding genes as well as a downregulation of GS2.1 and GS2.2 transcripts. Interestingly, plant metabolic responses at the root level did not follow the same pattern, with plants under combined deficit behaving similarly to W deficit plants, resulting in enhanced nitrate and proline concentrations, NR activity, and an upregulation of GS1 and NR genes than in CTR plants. Overall, our data suggest that the N remobilization and osmoregulation strategies play a relevant role in plant acclimation to these abiotic stresses and highlight the complexity of plant responses under a combined N+W deficit.

Cadmium effect on growth, ammonium assimilation, and amino acids levels in roots of Arabidopsis thaliana

Cadmium effect on growth, ammonium assimilation, and amino acids levels in roots of Arabidopsis thaliana

Adjunctive use of different lasers Er, Cr: YSGG, femtosecond, potassium titanyl phosphate and photodynamic therapy on radicular disinfection bonded to glass fiber post.

The aim of this study was to evaluate and compare the effect of different laser prototypes [Er, Cr: YSGG laser (ECYL), potassium titanyl phosphate laser (KTP), and Femtosecond laser (FSL)]and curcumin photosensitizer (CP) activated by Photodynamic therapy (PDT) on the bond strength of Pre-fabricated fiber reinforced composite (PFRC) post-bonded to radicular dentin. A total of fifty mandibular single-rooted closed apex teeth were extracted carefully, assembled, and decoronated up to the cementoenamel junction. The working length of all specimens was determined by using a 10 K patency file and later, were cleaned and shaped with Protaper NiTi system using the crown down approach, dried, and obturated with gutta-percha using an AH Plus sealer. Post space was prepared by guiding peeso-reamer. Based on the method of disinfection, the samples were allocated to five groups (n=10) at random: samples in group 1: curcumin photosensitizer (CP) activated by PDT, samples in group 2 disinfected using 5.25% NaOCl+17% EDTA, samples in group 3 disinfected using 5.25% NaOCl+17% EDTA+FSL, specimens in group 4 sterilized using 5.25% NaOCl+17% EDTA+KTP and samples in group 5 cleaned with 5.25% NaOCl+17% EDTA+ECYL. The fiber post was cemented via self-etch resin cement into the post space. All specimens with posts were dissected perpendicularly into apical, middle, and coronal dentin and subjected to the universal testing machine for push-out bond strength (PBS) testing. Statistical analysis was performed using a One-Way analysis of variance and Post Hoc Tukey multiple comparison tests. The highest PBS was corroborated when the radicular canal was disinfected with 5.25% NaOCl +17% EDTA+ ECYL at all three root levels (coronal, middle, and apical) and the lowest was adjudicated by decontamination with CP activated by PDT at all inspected root levels. Intergroup comparison presented that specimens in group 2: 5.25% NaOCl+17% EDTA (control) and group 4: 5.25% NaOCl+17% EDTA+KTP revealed comparable PBS outcome to group 5 (p>0.05) while samples in group 3 revealed the equivalent PBS values to group 1 (p<0.05) at all three root levels. Er, Cr: YSGG laser and potassium titanyl phosphate laser when used in combination with the conventional canal disinfection 5.25% NaOCl and 17% EDTA demonstrated the highest push-out bond strength values at coronal, middle, and apical levels of the root.

The Roles of Aerobic Exercise and Folate Supplementation in Hyperhomocysteinemia-Accelerated Atherosclerosis.

Hyperhomocysteinemia (HHcy) is an independent risk factor for atherosclerosis. Effective interventions to reduce HHcy-accelerated atherosclerosis are required. This study aimed to investigate the effects of aerobic exercise (AE) and folate (FA) supplementation on plasma homocysteine (Hcy) level and atherosclerosis development in a mouse model. Six-week-old female apoE-/- mice were grouped into five groups (N = 6-8): HHcy (1.8 g/L DL-homocysteine (DL-Hcy) in drinking water), HHcy + AE (1.8 g/L DL-Hcy and aerobic exercise training on a treadmill), HHcy + FA (1.8 g/L DL-Hcy and 0.006% folate in diet), HHcy + AE + FA (1.8 g/L DL-Hcy, 0.006% folate, and aerobic exercise training on a treadmill), and a control group (regular water and diet). All treatment was sustained for 8 weeks. Triglyceride, cholesterol, lipoprotein, and Hcy levels were determined enzymatically. Plaque and monocyte chemoattractant protein-1 (MCP-1) expression levels in mouse aortic roots were evaluated by immunohistochemistry. Compared to the HHcy group (18.88 ± 6.13 μmol/L), plasma Hcy concentration was significantly reduced in the HHcy + AE (14.79 ± 3.05 μmol/L, p = 0.04), HHcy + FA (9.4 ± 3.85 μmol/L, p < 0.001), and HHcy + AE + FA (9.33 ± 2.21 μmol/L, p < 0.001) groups. Significantly decreased aortic root plaque area and plaque burden were found in the HHcy + AE and HHcy + AE + FA groups compared to those in the HHcy group (both p < 0.05). Plasma MCP-1 level and MCP-1 expression in atherosclerotic lesions were significantly decreased in the HHcy + AE and HHcy + AE + FA groups compared to the HHcy group (all p < 0.05). AE reduced atherosclerosis development in HHcy apoE-/- mice independently of reducing Hcy levels. FA supplementation decreased plasma Hcy levels without attenuating HHcy-accelerated atherosclerosis. AE and FA supplementation have distinct mechanisms in benefiting atherosclerosis.

Commissural Alignment With ACURATE neo2 Valve in an Unselected Population.

Commissural alignment has become an important topic in transcatheter aortic valve replacement (TAVR) because it may improve coronary access, facilitate future valve procedures, and possibly improve valve durability. The efficacy of commissural alignment with ACURATE neo2 has not yet been shown in a large population. The authors sought to determine the feasibility and success of attempting commissural alignment in an unselected TAVR population treated with the ACURATE neo2 prosthetic heart valve. A total of 170 consecutive patients underwent TAVR with a dedicated implantation technique to align the TAVR valve to the native valve. Using right-left overlap and 3-cusp views, valve orientation was adjusted by rotation of the unexpanded valve at the level of the aortic root. Effectiveness was assessed postprocedure as the degree of misalignment determined by analyzing fluoroscopic valve orientation to corresponding cusp orientation on preprocedural computed tomography. Safety endpoints included mortality, stroke/transient ischemic attack, and additional complications through 30days. Of 170 patients, 167 (98.2%) could be analyzed for alignment, and all 170, for safety outcomes. Most patients (97%) had successful alignment (≤ mild misalignment), with 80% with commissural alignment, while the degrees of misalignment were 17% mild, 1.2% moderate, 1.8% severe. In this large evaluation of a commissural alignment technique, alignment was achieved in nearly all patients without safety concerns or impact to procedure duration. Commissural alignment appears effective and safe across all patients with this novel technique.

ENTREPRENEURIAL SUPPLY CHAIN STRATEGIES AT THE BOTTOM OF THE PYRAMID: A SUPPLY CHAIN RESOURCE ORCHESTRATION PERSPECTIVE

Bottom-of-the-pyramid (BOP) markets include an estimated 689 million people living in extreme poverty and, with some controversy, are frequently lauded as the “next frontier” for economic prosperity. In this context, supply chain resource orchestration (SCRO) could be a game changer; however, not enough is known about SCRO at the BOP. We examine SCRO theory and practice and identify strategies to enable more BOP players to achieve sustainable social and economic benefits. Our methodology involves: (1) a critical review of extant supply chain BOP literature to develop a SCRO model, (2) a content analysis with interview data triangulation that examines 21 case studies of business ventures at the BOP, (3) abstraction of a typology of common SCRO strategies, (4) development of a classification pyramid and SCRO process model that illustrate SCRO tactics across pyramid levels and (5) creation of a classification matrix, which encapsulates our observed connections among motivations, strategies, tactics and outcomes across the case studies. The research sheds light on opportunities for future scholars to examine supply chains and SCRO at the BOP level. It also provides practical guidance on how to orchestrate a successful BOP supply chain and suggests the SCRO strategies propagating from the grass roots level tend to benefit the most people.

A Study on Socio-economic Status of Tomato Growers in Solan District of Himachal Pradesh

Tomato is one of the most consumed and produced horticulture crops in the world. It is more economical than food grains due to their higher production per unit area in less time as they provide a good source of income to farmers. The present study has been conducted to study the socio-economic status of tomato growers in the Solan district of Himachal Pradesh. The multi-stage sampling technique and pre-tested interview schedule were used for data collection for the study. A sample was collected from eight villages in Solan district to make a total sample size of 80 tomato growers in the study area for the agricultural year 2021-2022. Mean, percentage, frequency, and standard deviation were used to find out the socio-economic status of tomato growers. The result revealed that majority i.e., 80 per cent of tomato growers belonged to middle aged group and 33.75 per cent had primary school education. About 76.25 per cent of tomato growers had joint family with 3 to 7 family members. 77.5 per cent of tomato growers had 0.5 to 3.17 bigha under tomato cultivation with 11 to 42 years of farming experience. The gross income of tomato growers was Rs. 60,000 per bigha and net income was Rs. 38,353 per bigha. The output input ratio worked out to be 1.77. The majority i.e., 55 per cent of tomato growers had medium level of socio-economic class. This study suggests the appointment of more extension personnel at grass root level. This study will be helpful in developing suitable and effective extension programmes to increase tomato production.

Taper 0.06 Versus Taper 0.04: The Impact on the Danger Zone

IntroductionThis study aimed to evaluate the effects of root canal enlargement on the danger zone (DZ) of mandibular molars. MethodsThirty mesial roots of mandibular first molars were scanned in micro-computed tomography (S1). Canals were sequentially enlarged with rotary instruments up to sizes 30/0.04 (S2) and 30/0.06 (S3). The dentin thickness was measured at 0.1-mm intervals after each preparation step (n = 2964 slices). Root level and position of the DZ were also recorded. Data were compared using analysis of variance with Bonferroni pairwise comparison, Cochran's Q method, and Pearson's test (α = 5%). ResultsComparing the specimens before (S1) and after (S2 and S3) preparations showed a significant reduction in the thickness of the DZ (P < .05), as well as between S2 and S3 steps (P < .05). At S1, the DZ was mostly located in the middle third of the root, but after preparation, it shifted toward the coronal direction (P < .05). Both S2 (P = .004, r = 0.508) and S3 (P = .004, r = 0.506) preparation steps showed a positive correlation between canal length and the root level of the DZ. At S1, the DZ was positioned toward the distal and mesial in 73.4% (n = 22) and 26.6% (n = 8) of the specimens, respectively. After S3, the number of specimens with DZ positioned toward the mesial aspect of the root significantly reduced to 3.3% (n = 1), whereas none of the specimens with DZ positioned toward the distal changed its position after root canal enlargements (P > .05). ConclusionOverall, the enlargement of mesial canals of mandibular first molars with final instruments sizes 30/0.04 and 30/0.06 affected the thickness, root level, and position of the DZ.

Exposure of suckling rats to hexavalent chromium (CrVI) alters bone formation at the base of the alveolus causing a delay in tooth eruption

ObjectivesHexavalent chromium (CrVI)-exposure of suckling rats has been shown to delay tooth eruption. However, the effects of CrVI-exposure on bone formation at the base of the alveolus, which provides a motive force for tooth eruption in the early stages of the process, remain unknown. The present work sought to evaluate the effect of CrVI-exposure on bone formation at the base of the alveolus during the intraosseous stage of tooth eruption in suckling rats, using histomorphometric and immunohistochemical studies. MethodsExperimental animals received 12.5 mg/kg-bw/day of potassium dichromate dissolved in saline solution by gavage starting on day 4 of the experiment; controls similarly received an equivalent volume of saline. All the animals were euthanized at the age of 9 days. The base of the developing alveolus at the level of the mesial root of the first lower molar was analyzed histomorphometrically and immunohistochemically. Data were statistically analyzed using student's t-test, with statistical significance set at p < 0.05. ResultsCrVI-exposed animals showed lower bone volume and height at the base of the alveolus, a significant preponderance of bone rest on the surface adjacent to the dental follicle, and a significantly lower percentage of positive Runt-related transcription factor-2 (RUNX2+) osteoblasts and a significantly higher number of mesenchymal-like RUNX2+ cells at the latter site. ConclusionCrVI-exposure during lactation affects bone formation at the base of the developing alveolus, delaying tooth eruption. These findings underscore the importance of controlling drinking water levels of toxic substances since their effects can alter the growth and development of individuals exposed during early childhood.

Diagnostic accuracy of Non-ECG Gated Chest CT (NEGCT) for cardiac chambers’ dimensions in comparison with echocardiography

Objective: To analyze the accuracy of Non-ECG Gated Chest CT (NEGCT) for cardiac chambers’ dimensions, keeping Echocardiography as the gold standard.&#x0D; Methods: After IRB approval, all patients that underwent concurrent contrast chest CT/CTPA and transthoracic 2D echocardiography exam at our institute within 1 month interval comprised the study population. Data was archived from HMIS PACS Database. The sizes of right ventricle (RV), left ventricle (LV) and left atrium (LA) was recorded in shot axis at axial CT sections and at echocardiography. RV and LV-transverse diameter was measured at basal third of the heart, from the inner to inner myocardium perpendicular to the inter-ventricular septum and LA-max. Anterior-posterior dimension was measured at the level of the aortic root.&#x0D; Results: Total 213 subjects were studied, with average age of 52.1±15.8 years. The CT-Echocardiography measurement concordance rates were significantly high for LA, LV and RV measuring 93.8%, 96.7% and 69.01% respectively. Using CT threshold for LA, LV, RV enlargement &gt; or equal to 45, 55, 35mm, the AUC via ROC curve analysis was estimated to be 0.912, 0.992, 0.650 respectively. The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and diagnostic accuracy of NEGCT was found to be 66.67%, 95.10%, 37.5%, 98.5% and 93.90% for LA; 100%, 96.67%, 30.0%, 100%, 96.7% for LV and 33.33%, 74.86%, 17.86% 87.26% and 69.01% for RV respectively.&#x0D; Conclusion: Overall, the accuracy of chamber dimensions at NEGCT was excellent with high CT-Echocardiography concordance, making it an alternative modality for examining chamber sizes, in the absence of cardiac MRI. Through it, a radiologist can also add more value to CT reporting.&#x0D; Keywords:&#x0D; Echocardiography, NEGCT, Dimensional Measurement

Seed priming by application of Microbacterium spp. strains for control of Botrytis cinerea and growth promotion of lettuce plants

The abusive use of chemical pesticides to control plant diseases has caused the loss of soil biodiversity as well as the emergence of resistance by plant pathogens. This effect has forced an urgent search for other environmentally sustainable alternatives that serve to control plant pathogens safely and efficiently. In this sense, the search for new biopesticides is currently a booming field within the field of agro-biotechnology. Volatile compounds produced by plant-associated microorganisms represent a diverse resource to promote plant growth and health. In this work, a collection of 29 strains of the genus Microbacterium from the composting of plant remains was studied. The objective was to determine whether the production of volatile compounds of microbial origin could contribute to the mitigation of symptoms caused by the fungus Botrytis cinerea (gray mold) as well as contribute to the lettuce plant growth in seedbeds. It should be noted that in both the B. cinerea inhibition and seed priming assays, the microorganisms did not come into direct contact with either the pathogen or the seeds. In all cases, incubation was carried out in a closed environment to expose the seeds to the microbial volatiles. Five strains capable of inhibiting the in vitro growth of B. cinerea at levels greater than 30% were pre-selected. Only 3 of these 5 strains, identified as M. aerolatum, M. foliorum and M. profundi showed positive results in the in vitro germination promotion test in lettuce seeds, increasing the radicle weight at levels close to 45%. These strains finally were tested in planta, showing two of them significant palliative effects of B. cinerea and promoting the development of lettuce seedlings both at aerial and root levels.

Enzymatic and Non-Enzymatic Antioxidant Responses of Young Tomato Plants (cv. Micro-Tom) to Single and Combined Mild Nitrogen and Water Deficit: Not the Sum of the Parts.

This study aims to perform a broad analysis of the antioxidant (AOX) responses of young tomato plants exposed to single and combined mild nitrogen (N) and water deficits through the evaluation of oxidative biomarkers, non-enzymatic and enzymatic AOX components. 'Micro-Tom' seedlings were subjected to four treatments: control (CTR; 100%N + 100%W), N deficit (N; 50%N), water deficit (W; 50%W), and combined deficits (N + W; 50%N + 50%W). An enhancement of several non-enzymatic and enzymatic components was found in plants subjected to N + W deficit, which presented higher anthocyanins accumulation (up to 103%) as well as higher levels of superoxide dismutase (SOD) transcripts at root level and of ascorbate peroxidase (APX) and catalase (CAT) transcripts at shoot level. This increase in the gene expression was also translated in augmented SOD (up to 202%), APX (up to 155%) and CAT (up to 108%) activity compared to CTR plants and the single deficits. Overall, tomato plants were able to employ defense strategies to cope with this combined deficit, as demonstrated by the higher total AOX capacity (up to 87%) compared to the single deficits, which contributed to the maintenance of their redox homeostasis, with unchanged values of lipid peroxidation and hydrogen peroxide compared with CTR plants.

The transcription factor IbNAC29 positively regulates the carotenoid accumulation in sweet potato.

Carotenoid is a tetraterpene pigment beneficial for human health. Although the carotenoid biosynthesis pathway has been extensively studied in plants, relatively little is known about their regulation in sweet potato. Previously, we conducted the transcriptome database of differentially expressed genes between the sweet potato (Ipomoea batatas) cultivar 'Weiduoli' and its high-carotenoid mutant 'HVB-3'. In this study, we selected one of these candidate genes, IbNAC29, for subsequent analyses. IbNAC29 belongs to the plant-specific NAC (NAM, ATAF1/2, and CUC2) transcription factor family. Relative IbNAC29 mRNA level in the HVB-3 storage roots was ~1.71-fold higher than Weiduoli. Additional experiments showed that the contents of α-carotene, lutein, β-carotene, zeaxanthin, and capsanthin are obviously increased in the storage roots of transgenic sweet potato plants overexpressing IbNAC29. Moreover, the levels of carotenoid biosynthesis genes in transgenic plants were also up-regulated. Nevertheless, yeast one-hybrid assays indicated that IbNAC29 could not directly bind to the promoters of these carotenoid biosynthesis genes. Furthermore, the level of IbSGR1 was down-regulated, whose homologous genes in tomato can negatively regulate carotene accumulation. Yeast three-hybrid analysis revealed that the IbNAC29-IbMYB1R1-IbAITR5 could form a regulatory module. Yeast one-hybrid, electrophoretic mobility shift assay, quantitative PCR analysis of chromatin immunoprecipitation and dual-luciferase reporter assay showed that IbAITR5 directly binds to and inhibits the promoter activity of IbSGR1, up-regulating carotenoid biosynthesis gene IbPSY. Taken together, IbNAC29 is a potential candidate gene for the genetic improvement of nutritive value in sweet potato.

Near-Infrared Fluorescent Carbon Nanotube Sensors for the Plant Hormone Family Gibberellins.

Gibberellins (GAs) are a class of phytohormones, important for plant growth, and very difficult to distinguish because of their similarity in chemical structures. Herein, we develop the first nanosensors for GAs by designing and engineering polymer-wrapped single-walled carbon nanotubes (SWNTs) with unique corona phases that selectively bind to bioactive GAs, GA3 and GA4, triggering near-infrared (NIR) fluorescence intensity changes. Using a new coupled Raman/NIR fluorimeter that enables self-referencing of nanosensor NIR fluorescence with its Raman G-band, we demonstrated detection of cellular GA in Arabidopsis, lettuce, and basil roots. The nanosensors reported increased endogenous GA levels in transgenic Arabidopsis mutants that overexpress GA and in emerging lateral roots. Our approach allows rapid spatiotemporal detection of GA across species. The reversible sensor captured the decreasing GA levels in salt-treated lettuce roots, which correlated remarkably with fresh weight changes. This work demonstrates the potential for nanosensors to solve longstanding problems in plant biotechnology.

Molecular characterization of SPL gene family during flower morphogenesis and regulation in blueberry

The SPL gene is a plant-specific transcription factor involved in the regulation of plant growth and development, which have been identified in woody plants. The process of floral bud differentiation affects the timing of flowering and fruit set and regulates plant growth, however, the mechanism of regulation of flower development by SPL genes is less studied. In this study, 56 VcSPL genes were identified in the tetraploid blueberry. The VcSPL gene family was classified into six subfamilies, and analysis of cis-elements showed that VcSPL genes were regulated by light, phytohormones (abscisic acid, MeJA), and low temperature. In the evolutionary analysis, segmental replication may play an important role in VcSPL gene amplification. Interestingly, we also studied diploid blueberry (Bilberry), in which 24 SPL genes were identified, and 36 homologous pairs were found, suggesting a high degree of convergence in the syntenic relationship between blueberry (Vaccinium corymbosum L) and bilberry (Vaccinium darrowii). Based on the expression profile, VcSPL genes were expressed at high levels in flowers, shoots, and roots, indicating a diversity of gene functions. Then we selected 20 differentially-expressed SPL genes to further investigate the role of VcSPL in floral induction and initiation. It showed that the genes VcSPL40, VcSPL35, VcSPL45, and VcSPL53 may play a crucial role in the blueberry floral transition phase (from vegetative growth to flower initiation). These results provided important information for understanding and exploring the role of VcSPLs in flower morphogenesis and plant growth.

Changes in root hydraulic conductivity in wheat (Triticum aestivum L.) in response to salt stress and day/night can best be explained through altered activity of aquaporins.

Salt stress reduces plant water flow during day and night. It is not known to which extent root hydraulic properties change in parallel. To test this idea, hydroponically grown wheat plants were grown at four levels of salt stress (50, 100, 150 and 200 mM NaCl) for 5-8d before harvest (d14-18) and subjected to a range of analyses to determine diurnal changes in hydraulic conductivity (Lp) at cell, root and plant level. Cell pressure probe analyses showed that the Lp of cortex cells was differentially affected by salt stress during day and night, and that the response to salt stress differed between the main axis of roots and lateral roots. The Aquaporin (AQP) inhibitor H2 O2 reduced Lp to a common, across treatments, level as observed in salt-stressed plants during the night. Analyses of transpiring plants and exuding root systems provided values of root Lp which were in the same range as values modeled based on cell-Lp. The results can best be explained through a change in root Lp in response to salt stress and day/night, which results from an altered activity of AQPs. qPCR gene expression analyses point to possible candidate AQP isoforms.