Exposure of suckling rats to hexavalent chromium (CrVI) alters bone formation at the base of the alveolus causing a delay in tooth eruption

Abstract

ObjectivesHexavalent chromium (CrVI)-exposure of suckling rats has been shown to delay tooth eruption. However, the effects of CrVI-exposure on bone formation at the base of the alveolus, which provides a motive force for tooth eruption in the early stages of the process, remain unknown. The present work sought to evaluate the effect of CrVI-exposure on bone formation at the base of the alveolus during the intraosseous stage of tooth eruption in suckling rats, using histomorphometric and immunohistochemical studies. MethodsExperimental animals received 12.5 mg/kg-bw/day of potassium dichromate dissolved in saline solution by gavage starting on day 4 of the experiment; controls similarly received an equivalent volume of saline. All the animals were euthanized at the age of 9 days. The base of the developing alveolus at the level of the mesial root of the first lower molar was analyzed histomorphometrically and immunohistochemically. Data were statistically analyzed using student's t-test, with statistical significance set at p < 0.05. ResultsCrVI-exposed animals showed lower bone volume and height at the base of the alveolus, a significant preponderance of bone rest on the surface adjacent to the dental follicle, and a significantly lower percentage of positive Runt-related transcription factor-2 (RUNX2+) osteoblasts and a significantly higher number of mesenchymal-like RUNX2+ cells at the latter site. ConclusionCrVI-exposure during lactation affects bone formation at the base of the developing alveolus, delaying tooth eruption. These findings underscore the importance of controlling drinking water levels of toxic substances since their effects can alter the growth and development of individuals exposed during early childhood.