Level Of Protection Research Articles

Level of prooxidant-antioxidant status in highly productive cows with comorbid obstetric, gynecological and orthopedic pathology

The lipid peroxidation and antioxidant defense system is a balanced system responsible for the processing and utilization of lipids in the body’s cells. It plays an important role in lipid metabolism, cell protection and overall health of the body. Its proper functioning is necessary to ensure optimal functioning of cells and organs throughout the body. The article clinically and experimentally substantiates the pathogenetic role of lipid peroxidation products and the level of antioxidant protection in cows with endometritis and purulent-necrotic processes in the finger area, as well as with the comorbid course of endometritis and orthopedic pathology. It has been shown that multimorbid manifestation is accompanied by a more severe course than individual diseases. It has been established that the development of postpartum endometritis and purulent-necrotic lesions of the limbs in cows is accompanied by a highly significant increase in lipid peroxidation products in the blood serum against the background of a decrease in the amount of antioxidant enzymes, with the exception of ceruloplasmin. Moreover, these changes are accompanied by a sharp jump in the comorbid course of endometritis and orthopedic pathology in highly productive animals.

Composites in Ballistic Applications Focused on Ballistic Vests—A Review

The development of ballistic materials has improved very rapidly in the last few years. Body armor plays an important role in protecting individuals during military threats. Body armor can be divided into hard and soft variants depending on the trade-offs between protection levels and wearer agility. Current research aims to optimize strength-to-weight ratios by using different combinations of synthetic or natural fibers or their combinations to achieve increasingly demanding requirements for ballistic materials. Moreover, it examines the various types of fibers utilized in the construction of body armor, ranging from traditional materials like metal and ceramic to synthetic and natural fibers. This paper discusses ongoing research efforts aimed at further enhancing the performance of these materials, such as the incorporation of modified natural fibers into advanced composite systems. The review provides a comprehensive analysis of the current state of the materials utilized in ballistic protection.

BLOCKCHAIN AND ITS APPLICATION IN THE DEFENCE SECTOR

The digital transformation of the economy affects many sectors, including the defence sector, which is sensitive from both a national and international perspective. The digitalisation of the economy is based on technologies such as robotics, autonomy, additive manufacturing technologies (3D printing), augmented reality, big data, quantum computers, artificial intelligence and blockchains. In this article, we will take a closer look at the latter, namely blockchain technology. We will briefly outline its main properties and advantages, which make it particularly attractive for use in the defence sector, and dwell on its application to implement such a tool as smart contracts. One of the key advantages of blockchain in the context of military operations is ensuring transparency and traceability of supplies. In today's realities, logistical problems can cost lives, but blockchain helps prevent fraud and improve coordination between interacting communication nodes. Thanks to the use of blockchain technologies, each unit of supply can be recorded in an immutable register, which allows you to track its path from sender to recipient. This reduces the risk of loss or theft, and simplifies the process of monitoring and verifying the accuracy of information about deliveries. In addition, the decentralised nature of the blockchain enhances the security of military communications, protecting them from interception and attack. Each message is encrypted in such a way that it can only be read by the addressee. This ensures a high level of confidentiality and data protection, as information transmitted through the blockchain is distributed among numerous network nodes, making it difficult for attackers to access. By using cryptographic methods, the blockchain ensures that even in the event of a successful attack, attackers will not be able to alter or falsify information. Creating a tamper-proof ID system will help fight counterfeiting and improve the process of identifying wounded or fallen soldiers. By assigning unique and unchangeable authenticators to personnel, equipment or weapons, one hundred per cent identification of military entities can be ensured. This allows for accurate tracking of the movement and status of each soldier and equipment, which contributes to more efficient resource management. This system also increases the level of trust in the information provided, as it is recorded in the blockchain and cannot be changed or deleted. The introduction of blockchain technology in the defence sector can bring significant benefits, including increased security, transparency and efficiency. However, this process is accompanied by numerous challenges and risks that need to be considered and overcome for successful integration.

ANALYSIS OF THE POSSIBILITY OF USING AN AUTONOMOUS MEASUREMENT SYSTEM AND REGISTRATION OF THE ASSESSMENT OF THE LEVEL OF PROTECTION OF THE ARMOURED VEHICLE CREW

In the context of large-scale armed aggression by the Russian Federation and growing losses from mine injuries, there is a significant need to obtain objective information about the level of protection provided by military armoured vehicles. At the same time, Ukrainian defence enterprises are constantly working to develop the latest methods to improve the protection of vehicle crews from the threat of mines and explosives. There is an acute problem and an essential need to quickly improve the mine resistance of standard models of armoured vehicles and, as a result, the need to quickly determine the effectiveness of the measures taken and make engineering decisions. The basis for the development of modern mine protection equipment should be to identify the impact of the shock wave not only on the vehicle structure, but primarily on the crew members. It is possible to evaluate the results, conduct comprehensive research and provide reasonable recommendations for further work only on the basis of the results of tests (experimental physical modelling) during which physical factors affecting the crew are measured. It is impossible to carry out the necessary measurements without the use of modern sensors (sensors) in conjunction with an information and measurement system that will ensure the reception, processing and registration of information. The main criteria for selecting sensors are sensitivity, resolution, linearity, frequency response, temperature response, noise level and long-term stability.

Assessment of PPMV-1 Genotype VI Virulence in Pigeons and Chickens and Protective Effectiveness of Paramyxovirus Vaccines in Pigeons.

Pigeon paramyxovirus serotype 1 (PPMV-1), an antigenic and host variant of avian paramyxovirus Newcastle disease virus (NDV), primarily originating from racing pigeons, has become a global panzootic. Egypt uses both inactivated PPMV-1 and conventional NDV vaccines to protect pigeons from disease and mortality. However, the impact of prevalent strains and the effectiveness of available vaccines in pigeons in Egypt are unclear. This study investigates the virulence of PPMV-1 (Pigeon/Egypt/Sharkia-19/2015/KX580988) and evaluates available paramyxovirus vaccines in protecting pigeons against a PPMV-1 challenge. Ten-day-old specific-pathogen-free (SPF) embryonated chicken eggs infected with this strain exhibited a mean death time (MDT) of 86.4 ± 5.88 h. The intracerebral pathogenicity index (ICPI) in day-old chickens was 0.8, while pigeons experienced an ICPI of 0.96 and an intravenous pathogenicity index (IVPI) of 2.11. These findings classify the strain as virulent and velogenic. Experimental infection of pigeons with this PPMV-1 strain at 106 EID50/0.1 mL resulted in a 62.5% mortality rate, displaying nervous and enteric distress. The virus caused extensive lesions in visceral organs, with strong immunohistochemistry signals in all examined organs, indicating the systemic spread of the virus concurrent to its neurotropic and viscerotropic tropism. Furthermore, vaccination using an inactivated PPMV-1 and live NDV LaSota vaccine regimen protected 100% of pigeons against mortality, while with a single NDV LaSota vaccine, it was 62.5%. The PPMV alone or combined with NDV LaSota induced protective levels of haemagglutination inhibition (HI) antibody titres and reduced virus shedding from buccal and cloacal cavities. Based on generalised linear gamma model analysis, both PPMV-1 and NDV LaSota are antigenically comparable by HI. These findings suggest that using both inactivated PPMV-1 (G-VI) and live attenuated NDV (LaSota) vaccines is an effective prophylactic regimen for preventing and controlling PPMV-1 and NDV in pigeons, thereby reducing the risk of interspecies transmission.

Improved influenza vaccine responses after expression of multiple viral glycoproteins from a single mRNA.

Influenza viruses cause substantial morbidity and mortality every year despite seasonal vaccination. mRNA-based vaccines have the potential to elicit more protective immune responses, but for maximal breadth and durability, it is desirable to deliver both the viral hemagglutinin and neuraminidase glycoproteins. Delivering multiple antigens individually, however, complicates manufacturingand increases cost, thus it would be beneficial to express both proteins from a single mRNA. Here, we develop an mRNA genetic configuration that allows the simultaneous expression of unmodified, full-length NA and HA proteins from a single open reading frame. We apply this approach to glycoproteins from contemporary influenza A and B viruses and, after vaccination, observe high levels of functional antibodies and protection from disease in female mouse and male ferret challenge models. This approach may further efforts to utilize mRNA technology to improve seasonal vaccine efficacy by efficiently delivering multiple viral antigens simultaneously and in their native state.

Individual and combined antagonism of aryl hydrocarbon receptor (AhR) and estrogen receptors (ERs) offers distinct level of protection against Bisphenol A (BPA)-induced pancreatic islet cell toxicity in mice.

Bisphenol A (BPA), a pervasive endocrine-disrupting chemical, is known to convey harmful impact on pancreatic islets through estrogen receptors (ERs). Conversely, BPA can activate aryl hydrocarbon receptor (AhR) in certain contexts and has raised concerns about potential toxicological effects. However, BPA-AhR interaction in the context of pancreatic islet toxicity is yet to be reported. We demonstrated the specific role of AhR and its interaction with ERs to mediate BPA toxicity in pancreatic islets. In vitro, isolated islet cells treated with BPA (1 nM), with or without CH22319 (10 mM) and ICI182780 (1 mM) and insulin release, glucose-stimulated insulin secretion (GSIS), cell viability, and pERK1/2 and pAkt expression were measured. In vivo, mice were treated with BPA (10 and 100 µg/kg body weight/day for 21 days) with or without intraperitonial co-treatment of CH22319 (AhR antagonist, 10mg/kg), and ICI182780 (ER antagonist, 500 µg/kg). Glucose homeostasis, insulin resistance, oxidative stress, and inflammatory markers were measured. In vitro data revealed the involvement of AhR in the BPA-mediated alteration in insulin secretion, GSIS, and pERK1/2 and pAkt expression which were counteracted by CH223191 (AhR antagonist) alone or with ICI182780 (ER antagonist). Further, CH223191 alone or with ICI182780 modulated BPA-induced oxidative stress and pro-inflammatory cytokines and alleviated islet cell dysfunction and impaired insulin secretion. In conclusion, therapeutic targeting of AhR and ER combined might be a promising target against diabetogenic action of BPA.

Biennial aerial application of Bacillus thuringiensis Berliner var. kurstaki is the most cost-effective approach of protection against spruce budworm (Choristoneura fumiferana [Clemens])

Key messageAerial application of Bacillus thuringiensis Berliner var. kurstaki (Btk) every second year to stands of white spruce (Picea glauca (Moench)Voss.), black spruce (Picea mariana Mill.) and balsam fir (Abies balsamea (L.) Mill.) is the most cost-effective spraying scenario for reducing the impact of spruce budworm (Choristoneura fumiferana Clemens) on wood production, providing a similar level of forest protection, but at lower cost, to the standard scenario currently used in which 50% of current year’s foliage is protected every year.ContextInsect outbreaks can have significant effects on forest productivity and various formulations of Bacillus thuringiensis Berliner var. kurstaki (Btk) are used to reduce their damage. In the Province of Québec, Canada, control programs aim to protect at least 50% of current-year foliage to limit tree mortality, but little information exists on the long-term cost-effectiveness of such programs.AimsOur goal was to evaluate the benefit/cost ratio and the efficacy of different Btk protection scenarios in reducing coniferous tree mortality and growth losses over a 11-year period. We hypothesized that less-intensive protection approaches (Btk applications every 2 or 3 years) may provide similar levels of protection but with higher cost-effectiveness ratios than the standard program currently used in Quebec.MethodsIn 2007, we established nineteen 100-ha experimental units in Quebec’s Côte-Nord region to determine the efficacy and cost-effectiveness of different Btk spraying scenarios for reducing tree mortality and volume losses in coniferous stands dominated by mature balsam fir trees (Abies balsamea (L.) Mill., with white spruce (Picea glauca (Moench) Voss.) and black spruce (P. mariana Mill.) as companion species. Tree mortality was monitored annually in three circular plots of 400 m2 within each experimental unit. Growth losses were evaluated using stem analyses.ResultsMortality was much higher in balsam fir than in black spruce and white spruce (respectively 74.4%, 13.8% and 5.9% in untreated stands) in all protection scenarios. The application of Btk every 2 years reduced balsam fir mortality to a level similar to the standard scenario (10.3% vs 7.15%, respectively) at a much lower cost. Growth losses have also been reduced but not to the same extent as in the standard scenario.ConclusionSpraying Btk every 2 years provides effective protection to balsam fir and is the most cost-effective scenario. A less intensive use of Btk would mitigate impact on non-target lepidoptera and allow protecting other areas, such as habitat of the woodland caribou, a threatened species which avoids disturbed areas.

A Robust Core-Shell Nanofabric with Personal Protection, Health Monitoring and Physical Comfort for Smart Sportswear.

Smart textiles with a high level of personal protection, health monitoring, physical comfort, and wearing durability are highly demanded in clothing for harsh application scenarios, such as modern sportswear. However, seamlessly integrating such a smart clothing system has been a long-sought but challenging goal. Herein, based on coaxial electrospinning techniques, a smart non-woven textile (Smart-NT) integrated with high impact resistance is developed, multisensory functions, and radiative cooling effects. This Smart-NT is comprised of core-shell nanofibers with an ionic conductive polymer sheath and an impact-stiffening polymer core. The soft smart textile, with a thickness of only 800µm, can attenuate over 60% of impact force, sense pressure stimulus with sensitivity up to 201.5 kPa-1, achieve temperature sensing resolution of 0.1°C, and reduce skin temperature by ≈17°C under a solar intensity of 1kWm-2. In addition, the stretchable Smart-NT is highly durable and robust, retaining its multifunction features over 10000 bending and multiple washing cycles. Finally, application scenarios are demonstrated for real-time health monitoring, body protection, and physical comfort of smart sportswear based on Smart-NT for outdoor sports. The strategy opens a new avenue for seamless integration of smart clothing systems.

Cramer Distance: A Deep Learning Approach for Better Epileptic Seizure Prediction

Epilepsy is a neurological condition that is found in most people all over the world, and the ability to accurately anticipate seizures in epileptic patients has a significant impact on both their level of protection and their overall quality of life. This research proposes a unique patient specific seizure prediction approach based on Deep Learning (DL) using long-term scalp electroencephalogram (EEG) recordings to predict seizure onset. Preictal brain states should be adequately detected and differentiated from the prevalent interictal brain states as early as possible to make this technology acceptable for real-time use. A single automated system has been designed for the Features Extraction (FE) and classification processes. The raw EEG signal that has not been pre-processed is considered the input to the system, and the signal is further reduced using subsequent computations. An innovative reconstruction approach using Variational Auto-Encoder Generative Adversarial Networks (VAE+C+GAN) with the Cramer Distance (CD) and a Temporal-Spatial-Frequency (TSF) loss function is presented in this research work. The machine that discriminates receives instructions to differentiate between created tests and actual samples, while the generator is verified to produce false samples that the discriminator does not recognize as fake. The proposed VAE+C+GAN’s experimental results have been examined, and a classification accuracy of 95% has been achieved. According to the experiment's findings, the VAE-C-GAN performs better than the current EEG classification system and has excellent potential for real-time applications.

Multiregional transcriptomic profiling provides improved prognostic insight in localized non-small cell lung cancer

Lung Cancer remains the leading cause of cancer deaths in the USA and worldwide. Non-small cell lung cancer (NSCLC) harbors high transcriptomic intratumor heterogeneity (RNA-ITH) that limits the reproducibility of expression-based prognostic models. In this study, we used multiregional RNA-seq data (880 tumor samples from 350 individuals) from both public (TRACERx) and internal (MDAMPLC) cohorts to investigate the effect of RNA-ITH on prognosis in localized NSCLC at the gene, signature, and tumor microenvironment levels. At the gene level, the maximal expression of hazardous genes (expression negatively associated with survival) but the minimal expression of protective genes (expression positively associated with survival) across different regions within a tumor were more prognostic than the average expression. Following that, we examined whether multiregional expression profiling can improve the performance of prognostic signatures. We investigated 11 gene signatures collected from previous publications and one signature developed in this study. For all of them, the prognostic prediction accuracy can be significantly improved by converting the regional expression of signature genes into sample-specific expression with a simple function—taking the maximal expression of hazardous genes and the minimal expression of protective genes. In the tumor microenvironment, we found a similar rule also seems applicable to immune ITH. We calculated the infiltration levels of major immune cell types in each region of a sample based on expression deconvolution. Prognostic analysis indicated that the region with the lowest infiltration level of protective or highest infiltration level of hazardous immune cells determined the prognosis of NSCLC patients. Our study highlighted the impact of RNA-ITH on the prognostication of NSCLC, which should be taken into consideration to optimize the design and application of expression-based prognostic biomarkers and models. Multiregional assays have the great potential to significantly improve their applications to prognostic stratification.

Ochrona informacyjna konsumenta w umowach ubezpieczenia

The article discusses issues related to consumer information protection in insurance contracts. The consumer’s right to information is explained and the legal nature of the insurer’s information obligation is presented. As a result of analyzing the information obligations constituting the basic instrument of consumer protection, appropriate conclusions were made regarding the implementation of these obligations. According to the author, the consumer information protection system is only partially implemented because the practice has shown shortcomings in its implementation. However, it must be agreed that the idea of protection through information is an appropriate assumption. Undoubtedly, the right direction is to increase the level of consumer information protection by clarifying regulations and such educational and information activities as recommendations issued by designated bodies.

Ionizable Cationic Lipids and Helper Lipids Synergistically Contribute to RNA Packing and Protection in Lipid-Based Nanomaterials.

Lipid-based nanomaterials are used as a common delivery vehicle for RNA therapeutics. They typically include a formulation containing ionizable cationic lipids, cholesterol, phospholipids, and a small molar fraction of PEGylated lipids. The ionizable cationic lipids are considered a crucial element of the formulation for the way they mediate interactions with the anionic RNA as a function of pH. Here, we show, by means of molecular dynamics simulation of lipid formulations containing two different ionizable cationic lipids (DLinDMA and DLinDAP), that the direct interactions of those lipids with RNA, taken alone, may not be sufficient to determine the level of protection and packaging of mRNA. Our simulations help and highlight how the collective behavior of the lipids in the formulation, which determines the ability to envelop the RNA, and the level of hydration of the lipid-RNA interface may also play a significant role. This allows the drawing of a hypothesis about the experimentally observed differences in the transfection efficiency of the two ionizable cationic lipids.

Topological protection revealed by real-time longitudinal and transverse transport measurements

Topology is essential for achieving unchanged (or protected) quantum properties in the presence of perturbations. A challenge facing the application is the variable protection levels displayed in real systems associated with the reconstructive behaviors of the dissipationless modes. Despite various insights on potential causes of backscattering, the edge-state-based approach is incomplete because the bulk states also contribute indispensably. This study investigates sample-scale reconstruction where dissipationless modes are global objects instead of being restricted to the sample edge. An integer quantum Hall effect hosted in a Corbino geometry is adopted and brought to the verge of a breakdown. Two independent and simultaneous detections are performed to capture transport responses in both longitudinal and transverse directions. The real-time correspondence between orthogonal results confirms two facts. 1. Dissipationless modes undergo frequent reconstruction in response to electrochemical potential changes, causing dissipationless current paths to expand transversely into the bulk while preserving chirality. A breakdown only occurs when a backscattering emerges between reconstructed dissipationless current paths bridging opposite edge contacts. 2. Topological protection is subject to an interplay of disorder, electron-electron interaction, and topology. The proposed reconstruction mechanism qualitatively explains the robustness variations, beneficial for protection optimization.

A Twin Transition in Regulatory Toxicology-moving towards Chemicals 2.0 and phasing out animal testing.

The European regulatory framework on chemicals is at a crossroads. There are calls for the framework to be more effective, by better protecting people and the environment. There is also room for it to be more efficient and cost-effective, by harmonising assessment practices across sectors and avoiding the need for unnecessary testing. At the same time, there is a political commitment to phase out animal testing in chemical safety assessments. In this commentary, we argue that these needs are not at odds with other. On the contrary, the roadmap to phase out animal testing could also be the transition pathway to a more efficient, effective and sustainable regulatory ecosystem. Central to our proposal is a framework based on biological reasoning in which biological questions can be answered by a choice of methods, with non-animal methods progressively becoming the only choice. Within this framework, a tiered approach to testing and assessment allows for greater efficiency and effectiveness, while also introducing considerations of proportionality and cost-effectiveness. Testing strategies, and their component methods, should be developed in tandem and judged in terms of their outcomes, the protection levels they inform, rather than their ability to predict the outputs of animal tests.

Семья как субъект правореализации на современном этапе развития российского законодательства

Today family as a collective subject of law enforcement performs a number of important for government functions: upbringing, educational, reproductive. Creation of conditions for full realization families` rights and opportunities becomes the State`s task. The article considers the problem of family recognition as an independent subject of law enforcement, reviews forms of rights realization by the family and its members, characterizes the main measures of state support, its influence on the level of legal protection and family well-being.

B-087 Sars-cov-2 Nt Chip test performance: a novel microarray-based enzymatic immunoassay for in vitro identification of neutralizing antibodies against emerging variants

Abstract Background The COVID-19 pandemic has created a need for reliable and adaptable diagnostic tools to detect exposure and neutralizing antibodies to emerging SARS-CoV-2 variants. To address this need, we evaluated the performance of the SARS-CoV-2 NT Chip Test, an ELISA-like immunoblot assay that incorporates a customizable panel of purified antigens from the variants of interest. This assay detects antibodies that neutralize the interaction between the receptor-binding domain (RBD) of the Wuhan strain and the Omicron BA1 variant with the cellular ACE-2 receptor and provides a marker of prior infection through the nucleocapsid protein. Methods We analyzed 73 clinical samples from six distinct groups: GROUP 1: Pre-pandemic samples (33); GROUP 2: Samples from children naive to Wuhan and infected with the Omicron variant (10); GROUP 3: Samples from individuals infected only with the original Wuhan strain (10); GROUP 4: Samples from individuals vaccinated and/or infected with the Wuhan strain and the Omicron variant (10); GROUP 5: Samples collected from individuals with 0-2 days after Omicron BA.1 infection (5) and GROUP 6: Samples collected from the same individuals 10 days after Omicron BA.1 infection (5). Evaluation utilized a proprietary non-numerical (qualitative) validation spreadsheet. Performance metrics included sensitivity, specificity, observed agreement (≥90%), and kappa index (≥0.70). Results were compared against the manufacturer's validation using the PRNT viral neutralization assay. Results Our comparative evaluation showed the following results for each parameter: 1) Wuhan neutralizing antibodies (sensitivity and specificity of 92%, agreement of 92%, and kappa index of 0.84); 2) Omicron neutralizing antibodies (sensitivity and specificity of 100%, agreement of 100%, and kappa index of 1.00); 3) Anti-nucleocapsid antibodies (sensitivity of 97%, specificity of 98%, agreement of 97%, and kappa index of 0.94). Three samples from GROUP 1 showed positive results for Wuhan RBD and one for Nucleocapsid, all close to the cutoff value. Four samples from GROUP 2 showed positive results for Wuhan RBD, which may be attributed to maternal antibodies. Three samples from GROUP 3 and three samples from GROUP 4, showed negative results for nucleocapsid, agreeing with the manufacturer's validation. Samples from GROUP 5 and GROUP 6 showed expected results, with Omicron RDB negative in GROUP 5, becoming positive in GROUP 6. Conclusions The test showed excellent performance in the verification of the three parameters in the populations studied, being approved in our laboratory. Due to its ability to incorporate antigens from emerging variants, it enables sensitive tracking of changes in neutralizing antibody profiles over time. The automated and customizable nature of the test provides an important tool for both public health epidemiological studies, monitoring changes in humoral immunity as this virus evolves, and for individual diagnostics, revealing the level of humoral protection of a patient against circulating strains in a timely manner to guide care. Although further studies are still warranted, the assay's ability to map evolving strain-specific serological immunity may offer valuable insights.

Improving the Impact Resistance of Anti-Ram Bollards Using Auxetic and Honeycomb Cellular Cores

Security is a crucial matter, and when it comes to road safety, barriers are increasingly needed to protect assets and pedestrians from intentional and accidental vehicular impacts. Hollow steel tubes are commonly used to produce bollards; however, their impact resistance and energy absorption are limited. Hence, the aim of this study is to investigate whether the addition of honeycomb and auxetic cellular cores can improve the energy absorption and protection level of existing bollards. Hollow bollard, a honeycomb–core bollard and an auxetic-core bollard were numerically modeled and tested (using Simulia Abaqus software, version 2019) against the impact of M1-class vehicles (of 1500 kg mass) at five different speeds (following PAS 68:2013 British standard). Hence, 15 cases/numerical models were considered, with 5 cases for each bollard type. The results revealed that the addition of an auxetic cellular core to the bollard system could increase its energy dissipation by 52% compared to the hollow steel bollard. Moreover, the proposed auxetic anti-ram bollard system was capable of stopping an M1-class vehicular impact of 64 km/h compared to only 32 km/h when using a hollow steel bollard. To the authors’ knowledge, the use of an auxetic core, explicitly for anti-ram bollards, can be considered the novel part of this research.

Immunogenicity and immune persistence of Zagreb 2-1-1 regimen of rabies vaccine in Chinese healthy individuals: A randomized, parallel-controlled of homologous vaccine with different immune procedure study

ABSTRACT This study was a randomized, parallel-controlled of homologous vaccines with different immune procedure research to evaluate the immunogenicity and immune persistence of Zagreb 2-1-1 regimen of rabies vaccine in Chinese healthy individuals. 240 subjects aged ≤ 20、21-50、≥51 y were randomly divided into 2 groups (1:1), Zagreb 2-1-1 regimen receivers as experimental group and Essen 5 regimen receivers as controlled group. Researchers collected venous blood of each subject before vaccine injection and on the day 7, 14, 42, 180, 365 after first dose. The immunogenicity and immune persistence was assessed by neutralizing antibody. The positive rate of neutralizing antibody in experimental group was 14.53% on the 7th day, and raised to 100% on the 14th day. It showed no significant difference between experimental and controlled group (P>0.05). Either in experimental or controlled group, GMC of neutralizing antibody was up to the peak on the 14th day, and it showed no significant difference between two groups (P>0.05). On the 42nd day, the antibody positive rate remained 100% with both Zagreb 2-1-1 and Essen 5 regimens, and the GMC of antibodies also remained high level. Then, on the 180th and 365th day with both regimens, the GMC of antibodies dropped dramatically, although it remained above the protective level of 0.5 IU/ml, the positive rates dropped to 84.40% and 84.11% (on the 180th day), and 61.29% and 58.62% (on the 365th day). Rabies vaccine injected by Zagreb 2-1-1 regimen can produce neutralizing antibody fastly and perdurably. Registration: ClinicalTrials.gov #NCT01821911and NCT01827917

GNSS positioning uncertainty prediction over a planned path considering signal attenuation for urban autonomous driving

Autonomous driving requires path planning to choose an optimal path to destination. Most works on path planning focus on the shortest travelling time, but neglect positioning condition of a path while positioning accuracy is significant for autonomous driving. In this work, we predict GNSS protection level (PL), which is positioning uncertainty upper bound, over planned paths to help choose the optimal path. We propose an improved stochastic model that considers the signal attenuation caused by trees and buildings using laser scanning point cloud. An adaptive strategy is proposed to linearly correlate the improved model and the carrier-to-noise-density ratio to make our stochastic model more practical. Finally, pseudorange-based RTD PL is calculated by advanced receiver autonomous integrity monitoring (ARAIM) using the planned path information and GNSS ephemeris. The experiments demonstrate that our method reduces the misleading rate by over 90% both in horizontal and vertical directions compared to the original stochastic model, with the lowest false alarm rates.