Branch Level Research Articles

Incidence of flow-limiting coronary artery stenoses in patients with diabetes - a coronary angiography natural history study

Abstract Background Diabetes is an established risk factor for the development of coronary atherosclerosis. We sought to assess the association between diabetes and the incidence of coronary artery atherosclerosis progression on coronary angiography. Methods Using the Swedish Coronary Angiography and Angioplasty Registry (SCAAR), we included all coronary artery segments with mild to no atherosclerosis defined as <50% luminal obstruction in patients undergoing a first-time coronary angiography in Sweden between 1989 and 2017. Patients with two or more vessel disease were excluded as were patients with a previous history of coronary artery disease. In patients with one vessel disease, the diseased vessel including all its branches were excluded. Patients were followed for 15 years until a clinically indicated re-angiography was done, death occurred or end of follow-up (April 2018). The primary outcome was plaque progression to flow-limiting coronary artery stenosis defined as ≥50% luminal obstruction on repeat angiography assessed using epidemiological tables, Kaplan Meier event rates, and Cox proportional hazard models. The importance of selected risk factors (smoking, hypertension, hyperlipidemia, and established coronary artery disease) was assessed. Results A total of 2 661 245 coronary segments in 248 736 patients were included in the study. Diabetes was present in 30 533 patients and 320 815 coronary artery segments. Kaplan-Meier event rates and incidence rates (IR) per 1000 segment-years were higher in patients with diabetes (5.5%, IR 2.78 [95% CI 2.70–2.85] vs. 3.0%, 1.44 [1.42–1.46]) resulting in an almost two-folded hazard ratio (HR) of plaque progression (HR 1.93 [95% CI 1.87– 2.00]). The anatomical distribution of plaque progression was similar between the two cohorts on all levels of analysis (coronary artery level, main artery and branches level, and coronary segment level artery). Diabetic patients with established coronary artery disease had a significantly higher risk of future events compared to those without clinically significant coronary artery disease. Conclusions Diabetes was associated with an almost twofold higher incidence rate of plaque progression but did not appear to alter the anatomical location of plaque development compared to the absence of diabetes. Once coronary artery disease is established, the rate of atherosclerotic events accelerates. Therefore, adopting aggressive primary prevention strategies is crucial to reduce the risk of adverse outcomes in diabetic patients.Event rates and anatomical distributionProgression of stenoses by risk factor

Optimization of tree-shaped fin structures towards enhanced discharging performance of metal hydride reactor for thermochemical heat storage based on entransy theory

Integrating fin structures into metal hydride (MH) bed can enhance heat discharging performance of the reactor for thermochemical heat storage. In this study, based on the entransy theory, the entransy dissipation rate (Eh,st) or the entransy dissipation (Eh,tr) of a simplified two-dimensional (2D) steady-state or transient heat conduction model for the MH bed was used to be an objective to optimize the tree-shaped fin (TSF) structures. Furthermore, response surface method (RSM) was adopted to explore the relationship between Eh,st or Eh,tr and structural parameters (i.e., branch number N, branch level M, length ratio α, width index β, and angle ratio γ). The results show that RSM can effectively predict influences of structural parameters of TSFs on heat discharging performance. Based on RSM with entransy theory, the obtained optimal combination parameters were N = 8, M = 3, α = 1, β = 0.5, and γ = 1, corresponding to the maximum gravimetric exergy-output rate (GEOR) of 20.98 W/kg. Furthermore, the GEOR with optimized fins was 11.5 % higher than that with unoptimized TSFs. The optimization method based on the entransy theory was effective to optimize the TSF structures by solving 2D model instead of three-dimensional model, and shorten the calculation time for optimization.

Traits coordination at leaf and branch level in response to the urban environment

The response of urban trees to the environment is the key content to explain the functional toughness of urban green space. However, how urban greening trees adapt to the dry and hot environment of the city remains unclear due to the single index and the limitations of the research area. We selected four representative cities in northern China with different regional dryness and measured 18 economic, hydraulic and anatomical traits of the common greening tree species (Fraxinus chinensis Roxb). The results show that there was generally a significant divergence in plant functional traits across urban dryness, and the traits have strong plasticity. As urban dryness increases, the number of leaf veins closed loops increases, forming a more complete network system of veins. While a significant decrease in the vessel vulnerability index further indicates the increased drought tolerance of urban trees to resist dry and hot environments. The increase of dryness leads to the decrease of vessel hydraulic diameter and area. The reduction in the vessel vulnerability index indicates an increase in the drought resistance of the twigs, which may be related to the input and distribution of resources in the branches and leaves. There was a strong correlation between plant economic-anatomical-hydrological traits. Under the stress of a dry urban environment, plants prefer to invest resources in the water sparing capacity of the stem, as well as in the construction of defence structures and nutrient storage capacity of the leaves.

In Silico Evaluation, Phylogenetic Analysis, and Structural Modeling of the Class II Hydrophobin Family from Different Fungal Phytopathogens.

The class II hydrophobin group (HFBII) is an extracellular group of proteins that contain the HFBII domain and eight conserved cysteine residues. These proteins are exclusively secreted by fungi and have multiple functions with a probable role as effectors. In the present study, a total of 45 amino acid sequences of hydrophobin class II proteins from different phytopathogenic fungi were retrieved from the NCBI database. We used the integration of well-designed bioinformatic tools to characterize and predict their physicochemical parameters, novel motifs, 3D structures, multiple sequence alignment (MSA), evolution, and functions as effector proteins through molecular docking. The results revealed new features for these protein members. The ProtParam tool detected the hydrophobicity properties of all proteins except for one hydrophilic protein (KAI3335996.1). Out of 45 proteins, six of them were detected as GPI-anchored proteins by the PredGPI server. Different 3D structure templates with high pTM scores were designed by Multifold v1, AlphaFold2, and trRosetta. Most of the studied proteins were anticipated as apoplastic effectors and matched with the ghyd5 gene of Fusarium graminearum as virulence factors. A protein-protein interaction (PPI) analysis unraveled the molecular function of this group as GTP-binding proteins, while a molecular docking analysis detected a chitin-binding effector role. From the MSA analysis, it was observed that the HFBII sequences shared conserved 2 Pro (P) and 2 Gly (G) amino acids besides the known eight conserved cysteine residues. The evolutionary analysis and phylogenetic tree provided evidence of episodic diversifying selection at the branch level using the aBSREL tool. A detailed in silico analysis of this family and the present findings will provide a better understanding of the HFBII characters and evolutionary relationships, which could be very useful in future studies.

Systematic review: Glycomics as diagnostic markers for hepatocellular carcinoma.

Hepatocellular carcinoma (HCC) is the most prevalent primary liver cancer with one of the highest cancer-related mortality rates worldwide. Early diagnosis is crucial for improving the therapeutic options and reducing the disease-related mortality. To investigate serum N-glycomics as diagnostic markers for HCC. We performed a comprehensive search in PubMed, EMBASE, Web of Science and Scopus through August 17, 2023. Eligible studies assessed the potential use of serum N-glycomics as diagnostic biomarkers for HCC. Study selection, data extraction and quality assessment were performed by two independent reviewers. Of the 48 articles included, 11 evaluated the utility of N-glycomics for the diagnosis of HCC in whole serum while the remaining articles focused on specific protein glycoforms or protein levels. Of these specific proteins, haptoglobin, alpha-fetoprotein (AFP), kininogen (Kin), α-1-antitrypsin and Golgi protein 73 (GP73) were the most frequently studied. Increased levels of fucosylation and branching presented as the most prevalent post-translational modifications of glycoproteins in patients with HCC compared to controls. Notably, glycomics-based biomarkers may provide a clinical benefit for the diagnosis of early HCC, as several algorithms achieved AUCs between 0.92-0.97. However, these were based on single studies with limited sample sizes and should therefore be validated. Alterations in serum N-glycomics, characterised by increased levels of fucosylation and branching, have potential as diagnostic biomarkers for HCC. Optimisation of study design, patient selection and analysing techniques are needed before clinical implementation will be possible.

Assessing the Allometric Scaling of Vectorized Branch Lengths of Trees with Terrestrial Laser Scanning and Quantitative Structure Modeling: A Case Study in Guyana

Allometric scaling is closely related to the morphology, function and behavior of trees, which are of great significance to the study of ecology. However, most of the traditional allometric scaling studies used the scalar attributes of trees, without considering the 3D vector mode of tree growth. In order to investigate the allometric relationships between branch lengths in 3D vector mode, in this study, an accurate and detailed quantitative structure model was used to reconstruct tree architectures from 3D point cloud data collected by terrestrial laser scanning and extract the structural parameters of each branch (length, branching level and zenith angle). The standardized major axis was used to establish and analyze the scalar and vector allometric relationships between branch lengths. Our results show that at the same branching level and using the same allometric model, the scaling exponents between the lengths of branches and the lengths of cumulative child (descendant) branches (no matter whether the lengths are in scalar or vector form) are similar among trees, and there is no significant difference between the scaling exponents of most trees. And the scaling exponents between the lengths of the horizontal components of branches and the cumulative lengths of the horizontal components of the child (descendant) branches are much larger than those between the lengths of the vertical components of branches and the cumulative lengths of vertical components of the child (descendant) branches. At different branching levels, the scaling exponents between the lengths of branches and the cumulative lengths of descendant branches tend to decrease with the increase in the branching level. The allometric models in terms of the cumulative lengths of horizontal components of the child (descendant) branches and the allometric models in terms of the cumulative lengths of child (descendant) branches have similar model accuracy and scaling exponents. The study results of allometric relationships between tree branch lengths in 3D mode are of great importance for understanding the crown morphology and the branching rule, which is helpful to further understand the growth strategies and adaptation mechanisms of trees and explain the growth and development mechanisms of trees from a physiological perspective.

Flowering induction in cassava using photoperiod extension premature pruning and plant growth regulators.

Cassava (Manihot esculenta Crantz) is a vital crop for food and economic security in many regions of the world. Despite the economic and social importance of cassava, challenges persist in developing superior varieties that meet the needs of farmers in terms of agronomic performance, nutritional quality, and resistance to pests and diseases. One of the main obstacles for genetic improvement is the lack of synchronization in flowering and the abortion of young flowers, making planned crosses and progeny production difficult. Therefore, the aim of this study was to evaluate the effect of photoperiod, premature pruning, and growth regulators on cassava flowering under low-altitude conditions in Brazil. Eight cassava clones with contrasting flowering capacity were assessed in Cruz das Almas, Bahia, using two photoperiods (ambient condition and extended photoperiod with red light for 12 hours), premature pruning at the first and second branching levels (with and without pruning), and the application of growth regulators: 0.5 mM 6-benzyladenine (BA) and 4.0 mM silver thiosulfate (STS) (with and without). Plots were assessed weekly for the number of female (NFF) and male (NMF) flowers, height of the first branching (H1B, in cm), number of days to the first branching (ND1B), and the number of branching events up to 240 days after planting (NOB). The extended photoperiod did not promote an increase in the number of flowers but allowed for precocity in cassava flowering, reducing the onset of flowering by up to 35 days, and significantly increasing the number of branches, which is closely related to flowering. The use of pruning and plant growth regulators (PGR) resulted in an increase in NFF from 2.2 (control) to 4.6 and NMF from 8.1 to 21.1 flowers. Therefore, under hot and humid tropical conditions at low altitudes in the Recôncavo of Bahia, manipulating the photoperiod and using premature pruning and plant growth regulators can accelerate cassava flowering, benefiting genetic improvement programs.

Akims of Villages of the Republic of Kazakhstan

The purpose is to study the mechanisms of improving the managerial efficiency of rural akims in Kazakhstan in the example of the Aktobe region. The ongoing reforms on the introduction of the institute of elections of rural akims, the decentralization of powers and the expansion of the competence of rural akims, and the introduction of independent rural budgets will strengthen the lower level of the executive branch of government. The effectiveness of the activity of akims is determined by the characteristics of the success of the professional activity of civil servants in terms of the degree of achievement of the planned result and the desired social effect, as well as the ratio of the results achieved with the resources spent on its implementation. The methods of observation, surveys, focus groups, analysis, systematization and generalization were used. In determining the effectiveness of the activities of akims by teaching methods - facilitation and brainstorming, a number of problems and ways to solve them have been identified. Materials on akims in the Department of Economy and Budget Planning and the Department of the Agency of Kazakhstan for Civil Service Affairs were also received. As the results of the study, the problematic issues identified during the training courses, seminars of rural akims of the Aktobe regional branch, and solutions identified by various active teaching methods are presented. Рossible solutions to the problematic issues identified during the survey and training are proposed. Research will be reflected in the scientific works of the doctoral student.

The Effectiveness of Medial Branch Blocks and Radiofrequency Neurotomy in Managing Chronic Thoracic Pain: A Systematic Review and Meta-Analysis

BACKGROUND: Extensive research into potential sources of thoracic pain with or without referred pain into the chest wall has demonstrated that thoracic facet joints can be a potential source of pain confirmed by precise, diagnostic blocks. The objective of this systematic review and meta-analysis is to evaluate the effectiveness of medial branch blocks and radiofrequency neurotomy as a therapeutic thoracic facet joint intervention. METHODS: Systematic review and meta-analysis of randomized controlled trials (RCTs) and observational studies of medial branch blocks and the radiofrequency neurotomy in managing thoracic pain utilizing the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) checklist was performed. A comprehensive literature search of multiple databases of RCTs and observational studies of medial branch blocks and radiofrequency neurotomy in managing chronic thoracic pain were identified from 1996 to December 2022 with inclusion of manual searches of the bibliography of known review articles and multiple databases. Methodologic quality and risk of bias assessment was also conducted. Evidence was synthesized utilizing principles of quality assessment and best evidence synthesis, with conventional and single meta-analysis. The primary outcome measure of success was 3 months of pain reduction for medial branch blocks and 6 months for radiofrequency thermoneurolysis for a single treatment. Short-term success was defined as up to 6 months and long-term was more than 6 months. RESULTS: This literature search yielded 11 studies meeting the inclusion criteria, of which 3 were RCTs and 8 were observational studies. Of the 3 RCTs, 2 of them assessed medial branch blocks and one trial assessed radiofrequency for thoracic pain. The evidence for managing thoracic pain with qualitative analysis and single-arm meta-analysis and GRADE system of appraisal, with the inclusion of 2 RCTs and 3 observational studies for medial branch blocks was Level II. For radiofrequency neurotomy, with the inclusion of one RCT of 20 patients in the treatment group and 5 observational studies, the evidence was Level III in managing thoracic pain. LIMITATIONS: There was a paucity of literature with RCTs and real-world pragmatic controlled trials. Even observational studies had small sample sizes providing inadequate clinically applicable results. In addition, there was heterogeneity of the available studies in terms of their inclusion and exclusion criteria, defining their endpoints and the effectiveness of the procedures. CONCLUSION: This systematic review and meta-analysis show Level II evidence of medial branch blocks and Level III evidence for radiofrequency neurotomy on a long-term basis in managing chronic thoracic pain. KEY WORDS: Chronic spinal pain, thoracic facet or zygapophysial joint pain, facet joint nerve blocks, medial branch blocks, controlled comparative local anesthetic blocks, diagnostic accuracy, radiofrequency neurotomy

MBL-1 and EEL-1 affect the splicing and protein levels of MEC-3 to control dendrite complexity.

Transcription factors (TFs) play critical roles in specifying many aspects of neuronal cell fate including dendritic morphology. How TFs are accurately regulated during neuronal morphogenesis is not fully understood. Here, we show that LIM homeodomain protein MEC-3, the key TF for C. elegans PVD dendrite morphogenesis, is regulated by both alternative splicing and an E3 ubiquitin ligase. The mec-3 gene generates several transcripts by alternative splicing. We find that mbl-1, the orthologue of the muscular dystrophy disease gene muscleblind-like (MBNL), is required for PVD dendrite arbor formation. Our data suggest mbl-1 regulates the alternative splicing of mec-3 to produce its long isoform. Deleting the long isoform of mec-3(deExon2) causes reduction of dendrite complexity. Through a genetic modifier screen, we find that mutation in the E3 ubiquitin ligase EEL-1 suppresses mbl-1 phenotype. eel-1 mutants also suppress mec-3(deExon2) mutant but not the mec-3 null phenotype. Loss of EEL-1 alone leads to excessive dendrite branches. Together, these results indicate that MEC-3 is fine-tuned by alternative splicing and the ubiquitin system to produce the optimal level of dendrite branches.

Vascular optimality dictates plant morphology away from Leonardo’s rule

Metabolic scaling theory (MST) provides an understanding of scaling in organismal morphology. Empirical data on the apparently universal pattern of tip-to-base conduit widening across vascular plants motivate a set of generalized MST (gMST) relationships allowing for variable rates of conduit coalescence and taper and a transition between transport and diffusive domains. Our model, with coalescence limited to the distalmost part of the conductive system, reconciles previous MST-based models and extends their applicability to the entire plant. We derive an inverse relationship between stem volume taper and conduit widening, which implies that plant morphology is dictated by vascular optimality and not the assumption of constant sapwood area across all branching levels, contradicting Leonardo's rule. Thus, energy efficiency controls conduit coalescence rate, lowering the carbon cost needed to sustain the vascular network. Our model shows that as a plant grows taller, it must increase conduit widening and coalescence, which may make it more vulnerable to drought. We calculated how our gMST model implies a lower carbon cost to sustain a similar network compared to previous MST-based models. We also show that gMST predicts the cross-sectional area of vessels and their frequency along the relative length better than previous MST models for a range of plant types. We encourage further research obtaining data that would allow testing other gMST predictions that remain unconfirmed empirically, such as conduit coalescence rate in stems. The premise of energy efficiency can potentially become instrumental to our understanding of plant carbon allocation.

Multi-objective optimization of fractal-tree microchannels in a rectangular heat sink by a distributed-adaptive genetic algorithm

Recently, an innovative heat dissipation technique of fractal-tree structures has attracted attention due to its excellent high heat transfer efficiency and low pumping power. Though the impact of geometrical parameters such as branching level and dimension ratios of successive branches on the heat transfer efficiency and pumping power are considered to be critical, the impact mechanisms are still not well studied and formulated. Hence, there is still no clear method to optimally arrange the fractal-tree microchannels (FTMCs) in a rectangular heat sink to achieve better thermal and hydraulic performance. Therefore, we developed a multi-objective optimization algorithm (distributed-adaptive genetic algorithm, DAGA) to optimize the different types of novel FTMCs concerning achieving an optimal COP (coefficient of performance, heat transfer/ pumping power). The developed DAGA can improve the computational efficiency and the quality of optimum by around 87.63% and 12.48%, respectively in the present work. Then, taking conventional rectangular parallel microchannels (RPMCs) as a reference, the COP of the optimized FTMCs in the same heat sink shows an enhancement of around 0.09 ∼ 0.57. The highest COP is achieved by the three-level branching FTMC. Furthermore, the optimized results reveal that branching level, bifurcation number, and microchannel width at the first branching level are sensible, while dimension ratio factors and microchannel length at the first branching level show low sensibility to the COP of FTMCs.

Requisite variety theory and third‐party guarantee loans: How local bank branches can reduce loan defaults

AbstractRequisite variety theory posits that internal variety is essential to develop responses to external variety. Facing increased local competition local bank branches could lower the odds of Small Business Administration (SBA) loan defaults by increasing knowledge variety through variations in geographic distances and industries among SBA loans. Our analysis shows that although local competition increases the odds of default, variations in industries represented in the SBA portfolio, the variation in geographic distances among loans in the portfolio lowers the odds of default. Our study emphasizes the importance of requisite variety at the branch level and presents important implications for policymakers and practitioners in the third‐party loan guarantee milieu.

Segregation patterns and transgressive segregation for agronomic characters in red chili (Capsicum annuum L.) Laris Variety of M3 generation after gamma-irradiation

The information on genetic parameters need to be known in relation to the selection process for breeding of high yielding plant genotypes. This study aims to estimate the frequency distribution, segregation pattern, and transgressive segregation for agronomic characters in M3 generation of red chili “Laris” variety after gamma-ray irradiation. The research was conducted at the Integrated Field Laboratory, Faculty of Agriculture, University of Lampung from September 2018 to May 2019. This research used single plant method. Data were analyzed using the chi-square test at the 1% level of significance for normal distribution fit and segregation pattern analysis. Transgressive segregation can be seen through the frequency distribution of M3 generation plants compared to M0. Results of this study indicated that the characters of dichotomous height, flowering age, harvesting age, plant height, branching level, number of fruits per plant, and fruit weight per plant had a normal distribution and associated with quantitative characters. The number of primary branches had a non-normal distribution and termed as a qualitative character. The segregation pattern of the number of primary branches follows a 15:1 ratio which indicates that the controlling gene is regulated by two genes that work in a double dominant epistatic manner. Transgressive segregation which has a positive value was found in the characters of dichotomous height, number of fruits per plant, and fruit weight per plant.

Three-dimensional spatial quantitative analysis of cardiac lymphatics in the mouse heart.

Three-dimensional (3D) microscopy and image data analysis are necessary for studying the morphology of cardiac lymphatic vessels (LyVs) and their association with other cell types. We aimed to develop a methodology for 3D multiplexed lightsheet microscopy and highly sensitive and quantitative image analysis to identify pathological remodeling in the 3D morphology of LyVs in young adult mouse hearts with familial hypertrophic cardiomyopathy (HCM). We developed a 3D lightsheet microscopy workflow providing a quick turn-around (as few as 5-6 days), multiplex fluorescence detection, and preservation of LyV structure and epitope markers. Hearts from non-transgenic and transgenic (TG) HCM mice were arrested in diastole, retrograde perfused, immunolabeled, optically cleared, and imaged. We built an image-processing pipeline to quantify LyV morphological parameters at the chamber and branch levels. Chamber-specific pathological alterations of LyVs were identified, and significant changes were seen in the right atrium (RA). TG hearts had a higher volume percent of ER-TR7+ fibroblasts and reticular fibers. In the RA, we found associations between ER-TR7+ volume percent and both LyV segment density and median diameter. This workflow and study enabled multi-scale analysis of pathological changes in cardiac LyVs of young adult mice, inviting ideas for research on LyVs in cardiac disease.

A STUDY ON CUSTOMER SATISFACTION ON SBI YONO WITH REFERENCE TO STONEHOUSE PET, NELLORE

In India, Banking industries has elaborated various financial rearrangements that would createa shift from all the traditional banking to digitalized banking services to gratify the customer’srequirement. Mobile banking increasing rapidly in India because customers find it more easy and comfortable way for doing banking transactions. Thus digitalized banking services has taken a step further in its digital platform through applications to access account information and other services provided by banks. This type of digitalized banking services will diminish branch level transaction and in due course of time it will make ease for customer services pressure, thus declines the operating cost for banks. SBI provides a lot of applications can be downloaded to the smart phones from app store and are free to experience the freedom to access anywhere anytime the banking services. SBI has several applications for their customers for ease transactions such as SBI ANYWHERE, SBI E-PAY, SBI QUICK, SBI BUDDY and SBIYONO. YONO is the latest application of SBI where the user’s savings bank account can be opened digitally using new YONO app and portal SBI. YONO is new app launched by State Bank of India.

THE SETTLEMENT OF MEDICAL ETHICS CODE INFRACTIONS AT RSUD Dr. SOEWANDHIE SURABAYA

Indonesian Honorary Council of Medical Ethics (MKEK) is one of the autonomous institutions of the Indonesian Medical Association that is formed especially at the national, regional, and branch levels to perform professional ombudsman duties, foster professional ethics and/or other institutional and ad hoc duties at their respective levels. The Medical Ethic Code in Indonesia has been regulated in the Decree of the Health Minister Number 434/2002 by the IDI management which has revised and established changes to the results of the national working meeting on medical ethics in 2001. The type of research used is empirical jurisdiction which in its research uses a problem approach regarding medical ethics code infractions at RSUD Dr. Soewandhie Surabaya. The data sources used are (1) primary data sources, such as observation and interviews; and (2) secondary data sources, such as data sources that support the primary data sources (law books, scientific papers, articles, and so on). This research technique used purposive sampling technique. The research results indicated that legal protection against violation of the medical ethic code can be resolved in two ways, such as the criminal process (litigation) and the civil process (non-litigation) in which the initial settlement process is assisted by the Ethics Honorary Council, then further handled by the Medical Ethics Committee through mediation related to the provision of sanctions.

Interactive effect of branch source-sink ratio and leaf aging on photosynthesis in pistachio.

Tree source-sink ratio has a predominant and complex impact on tree performance and can affect multiple physiological processes including vegetative and reproductive growth, water and nutrient use, photosynthesis, and productivity. In this study, we manipulated the branch level source-sink ratio by reduction of photosynthetic activity (partial branch defoliation) or thinning branch fruit load early in the growing season (after fruit set) in pistachio (Pistacia vera) trees. We then characterized the leaf photosynthetic light response curves through leaf aging. In addition, we determined changes in leaf non-structural carbohydrates (NSC) and nitrogen (N) concentrations. In leaves with high source-sink ratios, there was a gradual decrease in maximum net photosynthetic rate (ANmax) over the growing season, while in branches with low source-sink ratios, there was a sharp decline in ANmax in the first two weeks of August. Branches with high-sink showed an up-regulation (increase) in photosynthesis toward the end of July (at 1,500 growing degree days) during the period of rapid kernel growth rate and increased sink strength, with ANmax being about 7 μmol m-1 s-1 higher than in branches with low-sink. In August, low source-sink ratios precipitated leaf senescence, resulting in a drastic ANmax decline, from 25 to 8 μmol m-1 s-1 (70% drop in two weeks). This reduction was associated with the accumulation of NSC in the leaves from 20 to 30 mg g-1. The mechanisms of ANmax reduction differ between the two treatments. Lower photosynthetic rates of 8-10 μmol m-1 s-1 late in the season were associated with lower N levels in high-sink branches, suggesting N remobilization to the kernels. Lower photosynthesis late in the season was associated with lower respiration rates in low-source branches, indicating prioritization of assimilates to storage. These results can facilitate the adaptation of management practices to tree crop load changes in alternate bearing species.

Investigation into a perfect matching between fracture transfer performance and vertical well productivity based on multi-scale and total factor characteristic models

In fractured tight gas reservoirs, complex hydraulically induced fracture networks determine fluid flowing abilities of reservoirs. Thus, characterizing the complex fracture networks and revealing fracture transfer performances become a challenging work because of fracture branching, bending, and reversing in fractured reservoirs. In this paper, a fractal-like tree-shaped fracture model for gas transfer is proposed to optimize flow conductivity, pressure distribution at fracture intersection points, and flow transfer in such a complex fracture network considering the multi-scale effect, and total factor characteristics, including the length ratio, width ratio, branching angle, and branching level. The model is implemented to conduct validation against the experiment data in the literature. Subsequently, applying the proposed fracture model, the productivity equation for a vertical gas well intercepted by the fractal-like tree-shaped fracture network is further formulated. In addition, the good match with productivity simulation validates the accuracy of the developed productivity model for a vertical gas well. As a result, the impacts of the Knudsen number, fracture total factor characteristics, reservoir parameters, etc. on fracture transfer performances and well productivity are examined using the two proposed models. The results demonstrate that optimum fracture transfer performances do not match with the maximum well productivity. In contrast, the well productivity is jointly determined by both inlet flow volume of the fracture network and matrix permeability. Hence, a new formula considering both fracture characteristics and reservoir properties is proposed to match with the optimum fracture total length and maximum well productivity, thereby contributing to high-efficient and economic exploration of tight gas reservoirs.

Preliminary Results of Branch Level, Brachial Plexus Peripheral Nerve Stimulation on a Non-Human Primate.

Restoring functional hand control is a priority for those suffering from neurological impairments. Functional electrical stimulation (FES) is commonly applied to assist with rehabilitation. However, FES applied intramuscularly typically results in complex surgeries requiring many implants. This paper presents the preliminary findings from a feasibility study focused on evaluating the potential to access the upper extremity peripheral nerves through a single surgical approach (axillary approach). A single Japanese macaque (macaca fuscata) monkey was used to validate the feasibility of this study. Four of the five peripheral nerves which control the upper extremity were exposed, and had multi-contact epineural cuffs implanted: median, radial, ulnar and musculocutaneous. The axillary nerve was not accessible for epineural cuff placement with the current surgical approach used in this study. Electrical stimuli were used to produce movement contraction patterns of muscles relevant to the innervated peripheral nerves. In addition, to assist in quantifying the outcome, evoked potentials were simultaneously recorded from five extrinsic forearm flexors during median nerve stimulation. This feasibility study demonstrated that the axillary approach enables electrode placement to four of the five peripheral nerves required for upper extremity control through a single skin incision.Clinical relevance- This study demonstrated that the electrode placement to most of the peripheral nerves that control the arm and hand can be done by a single surgical approach: axillary approach.