Leopard Cat Research Articles

First photographic record of marbled cat Pardofelis marmorata Martin, 1837 (Mammalia, Carnivora, Felidae) in Nepal

The marbled catPardofelismarmoratais a Near Threatened small felid. The cat’s presence in Nepal is based on an anecdote. A camera trap-based study to access diversity and abundance of terrestrial mammals in eastern Nepal accumulated 3,014 camera trap days and resulted in 5,176 photographs of 17 medium-large sized mammal species. Amongst them, a marbled cat was captured at a single camera trap station in January 2018. The camera trap-capturing the marbled cat was located in the secondary forest at an altitude of 2,750 m a.s.l., dominated by free-ranging cattle close to a permanent human settlement (1.4 km) and a temporary cattle herding camp (0.4 km). This is the first photographic evidence of a marbled cat in Nepal. In this survey, we also recorded three other felid species: common leopardPantherapardus, Asiatic golden-catCatopumatemminckiiand leopard catPrionailurusbengalensis. We recommend detailed year-round camera trap surveys in the mid-hills of eastern Nepal along with research on adaptation of the small felids to human-dominated areas and assessment of immediate threats for preparing sound conservation management plans of the marbled cat and its sympatric species. Initiation of conservation programmes engaging localdokpa(herders) is necessary.

Dynamics of illegal wildlife trade in Indonesian markets over two decades, illustrated by trade in Sunda Leopard Cats

ABSTRACTWildlife trade is recognised as an impediment to conservation. Yet, long-term trade data are lacking for many species, precluding analysis of trends and trade dynamics. The Sunda Leopard Cat is endemic to insular Southeast Asia, where despite legal protection, it is openly traded. Data from Java and Bali (1996–2018, 393 surveys, 219 cats recorded in 21 markets) reveal that numbers of cats decreased (1.95/survey in the 1990s, 0.44/survey in the 2010s) and their prevalence decreased (from 46% to 30% of surveys recording cats). Invariably in the animal markets other protected wildlife was for sale, even if Sunda Leopard Cats were not present. Corrected for inflation, prices were higher in recent years (US$59 vs US$26) and higher in markets with low availability, possibly indicating an Anthropogenic Allee Effect whereby a premium is paid for rare species. Direct comparisons between markets reveal a complicated pattern with evidence of a significant decrease from only one market, but clear shifts of the trade between markets. Despite legal protection, we conclude that leopard cats are still commonly traded in Java, alongside other protected wildlife, albeit in smaller numbers than before.

Sex identification using ZFX and ZFY genes in leopard cats (Prionailurus bengalensis euptilurus) in Korea.

In this study, we used multiplex polymerase chain reaction (PCR) to determine the sex of leopard cats (Prionailurus bengalensis euptilurus) in Korea. When we applied PCR using primers based on the intronic region between ZFX and ZFY, only one DNA band of 143 bp was detected in females, compared with two specific bands of 143 and 310 bp in males, indicating that the band patterns were clearly different between females and males. Since the set of primers also proved compatible with tissue and fecal samples, the results of the present study demonstrate that the present PCR could be a convenient tool for identifying the sex of leopard cats.

Small cats in Nepal: Conservation status of felinae sub-family in the country

Twelve species of wild felids representing six lineages are found in Nepal, of which four are from Panthera lineages. Remaining eight species from non-panthera lineages is represented as poorly studied groups with limited information available for Nepal hindering effective conservation intervention. Thus, this review paper aimed to identify the status focusing on the distribution and threats of the cat species using review based approach. Among the small cats, jungle cat has the largest distribution range in Nepal, while occurrence of marbled cat has been verified recently from eastern Nepal. Fishing cats are recorded throughout low-lands of Nepal, while rusty spotted cats are recorded from western low-lands of Nepal and Asiatic golden cat have been documented from Mid-hills regions. Two species of small cat, i.e. Eurasian Lynx and Leopard Cat are protected by Nepalese law. Though there is a fair amount of information to make prediction about the distribution range, population level data are largely unavailable. Therefore, an explorative study on distribution followed by population level ecology in Nepal is essential.

Spatial organization and habitat selection of Geoffroy’s cat in the Espinal of central Argentina

We report data on the spatial ecology and habitat selection of eight adult Geoffroy’s cat Leopardus geoffroyi (five males and three females) that were radiotracked in an area of the central Argentine Espinal. Mean home range size varied from 2.2 ± 1.9 km2 (Kernel95%) to 2.8 ± 2.4 km2 (MCP100%), with male home ranges 4.1 larger than those of females. Core areas (Kernel50%) averaged 0.7 ± 0.7 km2 and were 3 times smaller for females. Daily movement patterns were consistent with the variations in home range sizes being greater in males (590.2 m ± 476.6) than females (413.5 m ± 288.1). Home range overlap averaged 38%, and was highly variable between males and females and reached its highest value for intersexual pairs. At second-order resolution, most of individuals had a strong selection for open woodland, while the other habitats were generally avoided. At the home range level, although the electivity index values for the open woodland were positive for all animals, habitat selection showed a high inter-individual variation. Our data support previous studies that suggest that Geoffroy’s cats show a certain degree of flexibility in their spatial behavior. Finally, we argue that natural woodland patches or habitats with dense vegetation are important for L. geoffroyi in the Argentinean Espinal and their alteration can affect the conservation status of this cat.

Periodic resource scarcity and potential for interspecific competition influences distribution of small carnivores in a seasonally dry tropical forest fragment

Small, isolated, and disturbed forest fragments potentially offer valuable habitats for small carnivore conservation. Yet the influence of resource availability—critical for survival and reproduction—on small carnivore habitat use within these modified environments is poorly understood. We conducted camera-trap surveys within a seasonally dry tropical forest fragment (148 km2) in northeastern Thailand to better understand the influence resource availability has on small carnivore habitat use within fragmented areas. Species occupancy models including both habitat and resource variables were assessed across periods of high and low resource availability (fruit abundance and rodent biomass). Species with similar resource or habitat preferences may also compete when resources are low. Therefore, we tested species with shared preferences for patterns of spatial co-occurrence and temporal overlap. Fruit availability influenced the distribution of common palm civet (Paradoxurus hermaphroditus), while rodent biomass influenced Asiatic jackal (Canis aureus), leopard cat (Prionailurus bengalensis), and small Indian civet (Viverricula indica), but only during annual periods of scarcity. In contrast, small Asian mongoose (Herpestes javanicus) was highly selective for a specific habitat (dry dipterocarp forest) regardless of seasonal fluctuations in food availability. Occupancy increased for all species during periods of resource scarcity, with leopard cat and Asiatic jackal experiencing the greatest increases (139% and 58%, respectively). Species with shared resource and habitat preferences appeared to avoid each other, either spatially or temporally. For example, leopard cat and Asiatic jackal co-occurred spatially less than would be expected, though only when rodents were scarce. Similarly, small Indian civet and small Asian mongoose, which used the same habitat and co-occurred spatially at a rate greater than expected, were active at different times. Our results indicate that seasonal resource fluctuations and interspecific interactions strongly influence the distribution of sympatric small carnivores in a fragmented forest.

Evaluating connectivity for two mid-sized mammals across modified riparian corridors with wildlife crossing monitoring and species distribution modeling

The movement of wildlife can be constrained by river development projects owing to the presence of artificial structures. This study analyzes the spatial riparian crossing patterns of two mammal species, the leopard cat (Felis bengalensis euptilura) and water deer (Hydropotes inermis). We examine their ability to traverse from habitat on one side of the river to the other, through riparian vegetation, embankments, and the river,. We used 34 months of monitoring on 104 stream segments with varying levels of human modification on the Seom river, South Korea, to analyze their riparian crossing preferences across the river between suitable habitats on either side of the steam. Environmental variables were used for two scales of analysis: a watershed scale and a stream segment scale that examined both riparian habitat suitability and riparian crossings. At the watershed scale, habitat suitability was determined using species distribution modeling. The resulting maps were inputted with other predictor variables to logistic regression models to predict areas of high probability for the species' occurences. For the river segment analyses, we determined species’ presences and potential crossings from snow tracking, heat sensor cameras, and scat. We compared the predicted crossing locations from the watershed scale model to observed crossings. The watershed models indicate that leopard cats prefer upland forest, while water deer prefer the forest edge and riparian corridor. For both target species, the best riparian habitats were characterized by the presence of vegetation cover on the embankment and by at least one side of an embankment being adjacent to farmland or forest cover. The two species showed different riparian crossing preferences. Water deer crossed through large culverts or under bridges, whereas leopard cats utilized drainage pipes and smaller culvert boxes. Stream reaches located close to a river tributary had the highest connectivity values, and areas modeled as good habitats were linked near riparian and watershed areas with high density for both species as well as culvert boxes with a high openness ratio. Artifacts such as steep banks, concrete embankments, and adjacent roads were found to degrade the riparian connectivity of wildlife. These outcomes can be used to identify suitable riparian wildlife habitats of mammals, to evaluate lateral connectivity of riparian corridors for mammals, and to develop criteria for river conservation and restoration in urbanizing areas and developing countries.

Assessing the spatiotemporal interactions of mesopredators in Sumatra's tropical rainforest.

Co-occurrence between mesopredators can be achieved by differentiation of prey, temporal activity, and spatial habitat use. The study of mesopredator interactions is a growing area of research in tropical forests and shedding new light on inter-guild competition between threatened vertebrate species that were previously little understood. Here, we investigate sympatry between the Sunda clouded leopard (Neofelis diardi) and Asiatic golden cat (Pardofelis temminckii) living in the Sumatran rainforests of Indonesia. We investigate: i) spatial overlap of predator-prey species using a combination of single-species occupancy modelling and Bayesian two-species modelling, while controlling for the possible influence of several confounding landscape variables; and, ii) temporal overlap between mesopredators and their shared prey through calculating their kernel density estimate associations. From four study areas, representing lowland, hill, sub-montane and montane forest, 28,404 camera trap nights were sampled. Clouded leopard and golden cat were respectively detected in 24.3% and 22.6% of the 292 sampling sites (camera stations) and co-occurred in 29.6% of the sites where they were detected. Golden cat occupancy was highest in the study area where clouded leopard occupancy was lowest and conversely lowest in the study area where clouded leopard occupancy was highest. However, our fine-scale (camera trap site) analyses found no evidence of avoidance between these two felid species. While both mesopredators exhibited highest spatial overlap with the larger-bodied prey species, temporal niche separation was also found. Clouded leopard was more nocturnal and, consequently, had higher temporal overlap with the more nocturnal prey species, such as porcupine and mouse deer, whereas the more diurnal golden cat had higher overlap with the strictly diurnal great argus pheasant. The Bayesian two species occupancy modelling approach applied in our study fills several important knowledge gaps of Sumatra’s lesser known mesopredators and provides a replicable methodology for studying interspecific competition for other small-medium sized carnivore species in the tropics.

Efficient immortalization of cells derived from critically endangered Tsushima leopard cat (Prionailurus bengalensis euptilurus) with expression of mutant CDK4, Cyclin D1, and telomerase reverse transcriptase

Tsushima leopard cat is the subspecies of Amur cats, and it is classified as the most highest class of critically endangered animals. Although the protection activity is highly recognized, the number of animals is decreasing due to the human activity and invasion of domestic cats and infectious disease. In this study, we succeeded primary culture of normal fibroblasts derived from the Tsushima leopard cat (Prionailurus bengalensis euptilurus). Furthermore, we introduced the human derived mutant Cyclin Dependent Kinase 4, Cyclin D1, and telomere reverse transcriptase. We showed that the expression of these three genes efficiently immortalized cells derived from Tsushima leopard cat. Furthermore, we showed that the chromosome pattern of the established cells is identical with the original one. These data indicate that our method of immortalization is useful to establish cell lines from critically endangered cats, which potentially contributes to the re-generation of critically endangered animals from cultured cell with reproductive technique, such as somatic cloning.

Molecular Genetic Findings of Spirometra decipiens and S. ranarum in Korea.

The taxonomy of Spirometra species has been controversial despite the medical and veterinary importance. Currently, only a few Spirometra species are considered valid species in the genus Spirometra. In the present study, the distribution of Spirometra species obtained from animals in Korea were identified by molecular analysis of the mitochondrial cytochrome c oxidase I (cox1) gene. A total of 28 Spirometra species specimens were analyzed. These were all collected between 1973 and 2008 in the Republic of Korea. Mitochondrial cox1 sequences were examined for a total of 28 specimens comprising 14 S. decipiens and 14 S. ranarum. The difference in partial cox1 sequences (316 bp) between S. erinaceieuropaei (KJ599680) and S. ranarum (this study) was 9.3%, while that between S. decipiens (KJ599679) and S. ranarum (this study) was 2.2%. Genetic analyses identified 2 Spirometra species in animals such as cat, leopard cat, dog, duck and snake in Korea as S. decipiens and S. ranarum. S. decipiens and S. ranarum were present in Gyeongnam Province (P), Jeonnam P, Gangwon P, Chungbuk P, and Seoul. S. decipiens was found in tadpoles, snakes, ducks, cats, leopard cats and dogs, while S. ranarum was found in cats and dogs. The ratio of S. decipiens:S. ranarum calculated from the molecular data was 14:14 (or 1:1). These results indicate that S. decipiens and S. ranarum are sympatrically distributed in Korea.

Primary study of seroprevalence to virus pathogens in wild felids of South Primorie, Russia

Seroprevalence to nine different virus pathogens was estimated for Russian big cats (Amur tiger (Panthera tigris altaica Temminck, 1844) and far-eastern leopard (Panthera pardus orientalis (Schiegel, 1857))) in Southern Primorie, Russia (n = 25), in 2008–2016. Serum samples from smaller cats (Eurasian lynx (Lynx lynx (Linnaeus, 1758)) and far-eastern wildcat (leopard cat) (Prionailurus bengalensis euptilurus (Elliot, 1871))) were also tested for these pathogens (n = 19) during the same period. Felids of Russian Southern Primorie showed seroprevalence to eight out of nine tested pathogens, including highly dangerous feline immunodeficiency virus, feline leukemia virus, and canine distemper virus. Antibodies to feline panleukopenia virus were found to be much more widespread in cats (45%) than antibodies to any other virus. They were detected in samples taken from tigers, leopards, and far-eastern wildcats but not lynxes. Antibodies to pseudorabies virus were detected only in Amur tiger (29%), whose main prey is the most common carrier of the virus (wild boar), unlike for the other studied cats’ species.

<b>Small carnivores of Silent Valley National Park, Kerala, India</b>

A study on the small carnivores in Silent Valley National Park (SVNP), southern Western Ghats, Kerala, India was conducted from September 2015 to April 2016, using the camera trap technique. Seven species of small carnivores were recorded during the study. The most common species of small carnivore of SVNP was Viverricula indica (44%) followed by Paradoxurus jerdoni (20%) and Herpestes vitticollis (17%). The other small carnivores found at SVNP were Herpestes fuscus (7%), Prionailurus bengalensis (6%), Aonyx cinereus (5%) and Martes gwatkinsii (1%). P. jerdoni and M. gwatkinsii are endemic to the Western Ghats. We discuss the niche partitioning among small carnivores in SVNP.

Identification of Felis catus Gammaherpesvirus 1 in Tsushima Leopard Cats (Prionailurus bengalensis euptilurus) on Tsushima Island, Japan.

Felis catus gammaherpesvirus 1 (FcaGHV1) is a widely endemic infection of domestic cats. Current epidemiological data identify domestic cats as the sole natural host for FcaGHV1. The Tsushima leopard cat (TLC; Prionailurus bengalensis euptilurus) is a critically endangered species that lives only on Tsushima Island, Nagasaki, Japan. Nested PCR was used to test the blood or spleen of 89 TLCs for FcaGHV1 DNA; three (3.37%; 95% CI, 0.70–9.54) were positive. For TLC management purposes, we also screened domestic cats and the virus was detected in 13.02% (95% CI, 8.83–18.27) of 215 cats. Regarding phylogeny, the partial sequences of FcaGHV1 from domestic cats and TLCs formed one cluster, indicating that similar strains circulate in both populations. In domestic cats, we found no significant difference in FcaGHV1 detection in feline immunodeficiency virus-infected (p = 0.080) or feline leukemia virus-infected (p = 0.163) cats, but males were significantly more likely to be FcaGHV1 positive (odds ratio, 5.86; 95% CI, 2.27–15.14) than females. The higher frequency of FcaGHV1 detection in domestic cats than TLCs, and the location of the viral DNA sequences from both cats within the same genetic cluster suggests that virus transmission from domestic cats to TLCs is likely.

Characterization and cryopreservation of Amur leopard cats (Prionailurus bengalensis euptilurus) semen collected by urethral catheterization

The Amur leopard cat (Prionailurus bengalensis euptilurus) is a globally endangered species, and there is thus an urgent need to increase its population. The objectives of this study were to: (1) confirm the utility of urethral catheterization (UC) for semen collection from Amur leopard cats; (2) investigate proper dilution media for fresh semen; and (3) investigate the effectiveness of sperm cryopreservation, including examining the effect of glycerol concentration. Six adult males in captivity (mean weight 5.03 ± 0.44 kg, aged 2–6 years) were included. All study procedures were performed during the breeding season (February to April) over two consecutive years. Semen samples (n = 28) were collected four or five times from each animal (four times for two animals and five times for four animals) by UC under general anaesthesia, and their characteristics (including sperm motility) were evaluated. First, the sperm motility in semen diluted in Ham's F-10 or phosphate buffer saline (PBS) was compared. Next, semen diluted with TEST-yolk buffer containing 2%, 4%, or 6% glycerol was frozen in a liquid nitrogen tank, and sperm motility and acrosome integrity were evaluated after thawing. No difference in motility was observed between sperm diluted in Ham's F-10 and PBS. The percentages of sperm motility and kinetic values in semen frozen in 2% or 4% glycerol were higher than those in semen stored in 6% glycerol. In conclusion, the UC method for semen collection is recommendable for Amur leopard cats and should be useful for artificial insemination. Although sperm motility decreased after thawing, samples thus preserved may be usable for advanced reproductive techniques, such as intracytoplasmic sperm injection or in-vitro fertilization.

Sperm cryopreservation in the Far-Eastern wildcat (Prionailurus bengalensis euptilurus).

The Far-Eastern wildcat (Prionailurus bengalensis euptilurus) is a rare and poorly investigated nondomestic felid species. An attempt of freezing and cryopreserving Far-Eastern wildcat spermatozoa in CaniPlus Freeze (CPF) medium is reported. Sperm was collected by electroejaculation from five adult Far-Eastern wildcat captive-born males. Epididymal spermatozoa from five adult randomly bred domestic cat males were used as a reference. The viability of frozen-thawed spermatozoa evaluated by double staining with SYBR Green I and PI followed by the subsequent confocal laser scanning microscopy (CLSM) was 38.2%±3.0% for the domestic cat and 38.0%±10.2% for the Far-Eastern wildcat. The motility of frozen-thawed spermatozoa was 30.8%±9.8% for the domestic cat and 33.7%±15.1% for the Far-Eastern wildcat. Sperm morphology was assessed by light microscopy. The total percentage of normal spermatozoa after freezing and thawing was 51.9±5.9 for the domestic cat and 55.0%±6.4% for the Far-Eastern wildcat. Defects of flagella were the most frequently observed abnormalities in both species (32.2%±4.8% and 30.8%±4.4% of all reported anomalies for the domestic cat and Far-Eastern wildcat, respectively). Domestic cat epididymal and Far-Eastern ejaculatory spermatozoa fertilized in vitro-matured oocytes of the domestic cat (30.0%±5.5% and 35.5%±15.0%, respectively). Taken together, these results suggest that the freezing of Far-Eastern wildcat spermatozoa with CPF medium is a suitable method for Felidae cryopreservation.

Dermatopatias associadas a micose e sarna superficiais em dois gatos-maracajás (Leopardus wiedii) no Zoológico Nacional da Nicarágua

The margay cat inhabits preserved tropical forests. Its geographical distribution extents from the north of Mexico, Central America, Peru, Paraguay, Ecuador and Brazil. We report the therapeutic management and resolution of clinical signs in two margay cats (Leopardus wiedii), who presented skin lesions characterized by areas of alopecia predominantly located on the back, ears, neck and front legs. Microscopic evaluation of skin scrapings revealed the presence of mites identified as Notoedres sp and Sarcoptes sp, as well as the presence of artrospores. Treatment consisted of administration of itraconazole (5 mg/kg PO, 24 h) and ivermectin (300 mcg SC). The animals were transferred to a new enclosure previously treated with iodine and chlorine. After 11 weeks of treatment there was complete remission of lesions, and no recurrence was observed.

The method matters: The effect of handling time on cortisol level and blood parameters in wild cats.

Blood analysis has recently become a popular tool to assess the welfare of the wild cats. However, the estimates of blood parameters may depend on the sampling method. We have tested (1) if the sampling procedure influences blood parameters and (2) what parameters are the most efficient in assessing the physiological status in wild cat species. We assessed the effect of handling time on red blood cells (RBC) and white blood cells (WBC) counts, the ratio of neutrophils to lymphocytes (N/L ratio), and serum cortisol level within 1hr after the capture of the animal in six far-east wild cats (Prionailurus bengalensis euptilura). Also, we analyzed literature data in 17 cat species to assess the effect of place of study, type of immobilization, and handling time on WBC count and N/L ratio. Serum cortisol level varied significantly with the handling time. RBC and WBC counts were strongly affected by the handling time. N/L ratio was very robust and did not depend on the handling time. However, the analysis of literature data has shown that the prolonged handling time (over 1hr) and the type of immobilization significantly influence the N/L ratio, whereas the WBC count does not depend on any of considered factors. We conclude that while most blood parameters of cats are affected by routine handling time, the N/L ratio does not vary if the samples are collected within 1hr after the capture of the animal. All other tested parameters should be treated with caution.

Microsatellite DNA analysis reveals lower than expected genetic diversity in the threatened leopard cat (Prionailurus bengalensis) in South Korea.

To optimize conservation efforts, it is necessary to determine the risk of extinction by collecting reliable population information for a given species. We developed eight novel, polymorphic microsatellite markers and used these markers in conjunction with twelve existing markers to measure genetic diversity of South Korean populations of leopard cat (Prionailurus bengalensis), a species for which population size and habitat area data are unknown in the country, to assess its conservation status. The average number of alleles and the observed heterozygosity of the species were 3.8 and 0.41, respectively, and microsatellite diversity was lower than the average genetic diversity of 57 populations of 12 other felid species, and lower than that of other mammal populations occurring in South Korea, including the raccoon dog (Nyctereutes procyonoides), water deer (Hydropotes inermis), and endangered long-tailed goral (Naemorhedus caudatus). Furthermore, analysis of genetic structure in the national leopard cat population showed no clear genetic differentiation, suggesting that it is not necessary to divide the South Korean leopard cat population into multiple management units for the purposes of conservation. These results indicate that the genetic diversity of the leopard cat in South Korea is unexpectedly low, and that the risk of local extinction is, as a result, substantial. Thus, it is necessary to begin appropriate conservation efforts at a national level to conserve the leopard cat population in South Korea.

<b>Small carnivores of Wayanad Wildlife Sanctuary, the southern Western Ghats, India</b>

The small carnivores of Wayanad Wildlife Sanctuary were surveyed using camera traps. The study was conducted between November 2016 and February 2017 at 111 camera trap locations. Twenty-five mammal species were recorded, including nine small carnivores from the Mustelidae, Viverridae, Herpestidae and Felidae families. Species included the Asian Small-clawed Otter Aonyx cinereus, Brown Palm Civet Paradoxurus jerdoni, Common Palm Civet Paradoxurus hermaphroditus, Small Indian Civet Viverricula indica, Indian Grey Mongoose Herpestes edwardsii, Ruddy Mongoose Herpestes smithii, Stripe-necked Mongoose Herpestes vitticollis, Jungle Cat Felis chaus and Leopard Cat Prionailurus bengalensis. The most common small carnivore was Viverricula indica, followed by Herpestes vitticollis and Paradoxurus hermaphroditus. Time activity patterns revealed that the viverrids were nocturnal while the herpestids were diurnal. The sanctuary supports one endemic species of small carnivore, Paradoxurus jerdoni, and one threatened species, Aonyx cinereus, which is Vulnerable according to the IUCN Red List of Threatened Species. This documentation highlights the biodiversity significance of Wayanad Wildlife Sanctuary and the Nilgiri mountain range in the Western Ghats.

Short Communication: Javan Leopard Cat (Prionailurus bengalensis javanensis Desmarest, 1816) in the Cisokan non-conservation forest areas, Cianjur, West Java, Indonesia

Shanidah SS, Partasasmita R, Hudoso T. Parikesit, Meganatara EN. 2018. Short Communication: Javan Leopard Cat (Prionailurus bengalensis javanensis Desmarest, 1816) in the Cisokan non-conservation forest areas, Cianjur, West Java, Indonesia. Biodiversitas 19: 37-41. Javan Leopard Cat (Prionailurus bengalensis javanensis Desmarest, 1816) in the Cisokan non-conservation forest areas, Cianjur, West Java, Indonesia. Biodiversitas 19: xx, xxx.There is no accurate data about the number of leopard individuals in Java Island in both conservation and non-conservation areas. Besides, human continue to deforestation for the necessities of life, such as agriculture, plantation, etc. Therefore, it is important to research about the existence of leopard cat in Cisokan of Cianjur Regency. The aim of this research is to investigate evidence of the leopard cat’s existence, encounter rate of leopard cat and estimation of leopard cat individual in the non-conservation forest areas, Cisokan, Cianjur. Indirect observation sign survey and direct observation with camera trap were carried out to find evidence of the existence of leopard cat (feces and footprint). The results of the study showed that the evidence of leopard cat’s existence are 6 footprints, 27 feces, and 1 individual which was able to observe. The encounter with camera traps was 29.16 independent detections /100 camera days, resulting in 7-12 estimated individuals.