As a result of long term studies of collection material of five pulses (pea, soybean, bean, chickpea, lentil), samples were grouped into three classes, depending on their level of biological performance (low, medium and high).Purpose. Classification of collection samples of pulses from the base collection of plant genetic resources ofUkraine according to their biological performance, investigation of its structural organization, identification of valuable sources.Material and Methods. The results of long-term (17 years) studies in field and laboratory experiments in breeding crop rotation 1 at the Plant Production Institute nd. a V. Ya. Yuryev NAAS are presented. The study material was collection samples. Over the study period 4,813 samples were estimated in a three-year cycle, including 1,390 pea samples, 821 soybean samples, 1,126 bean samples, 836 chickpea samples, and 640 lentil samples. Collection samples of pulses were investigated according to the "Guidelines of the All-Russian Research Institute of Plant Breeding for Studying Pulses" (1975).Results and Discussion. We analyzed the formation of biological performance of collection samples of pulses, depending on weather conditions and ripeness groups. The studied samples were grouped into three classes (according to classifiers of the corresponding genera) – with low, medium and high yield capacity. Most of the pea collection samples had medium (47.8%) and high (38.6%) biological performance, soybean, chickpea and bean samples were characterized by low yield capacity, lentil samples – by medium yield capacity. Pea, bean, lentil, and chickpea samples belonging to the middle-ripening group and soybean samples from the early season group had a high level of biological performance.Conclusions. The studied collection samples from the base collection of five pulses in terms of biological performance and its constituent elements are a representative selection of source material for the creation of pea, soybean, bean, chickpea, and lentils varieties of various ripeness groups with high biological yield capacity.