The spectral properties of a singular left-definite Sturm-Liouville operator JA are investigated and described via the properties of the corresponding right-definite selfadjoint counterpart A which is obtained by substituting the indefinite weight function by its absolute value. The spectrum of the J -selfadjoint operator JA is real and it follows that an interval .a; b/ � R C is a gap in the essential spectrum of A if and only if both intervals .� b; � a/ and .a; b/ are gaps in the essential spectrum of the J -selfadjoint operator JA. As one of the main results it is shown that the number of eigenvalues of JA in .� b; � a/ ( .a; b/ differs at most by three from the number of eigenvalues of A in the gap .a; b/; as a byproduct results on the accumulation of eigenvalues of singular left-definite Sturm-Liouville operators are obtained. Furthermore, left-definite problems with symmetric and periodic coefficients are treated, and several examples are included to illustrate the general results.