The purpose of this paper is to analyse the effect that on aerobics online courses will have on university students’ learning satisfaction, and the mediating role that learning engagement and learning motivation will play between aerobics online courses and university students. 1,000 university students majoring in “aerobics” were used as the research subjects. Afterwards, the explanatory variable was “aerobics”, the explanatory variable was “university student learning satisfaction”, and the mediating variables were “learning engagement” and “learning motivation”. The data obtained from the questionnaire survey were analyzed empirically using descriptive statistics, reliability test, analysis of variance, correlation analysis, regression analysis and validation analysis of mediating variables. Based on the results, first, there are significant gender and grade differences in college students, college students’ learning satisfaction, college students’ learning engagement and college students’ motivation. Second, there are negative correlations between college students’ and college students’ learning satisfaction, college students’ learning satisfaction, college students’ learning engagement and college students’ learning motivation. Third, the regression results showed that the stress of the online aerobics course has a negative impact on the satisfaction of the students, and the higher the stress felt by the students in the online aerobics course, the lower the learning engagement and learning motivation of the students and the lower the learning satisfaction of the students. As the conclusion, universities should focus on improving the teaching format of aerobics online courses to relieve the stress of university students, improve their commitment, motivate them to learn and achieve an increase in their satisfaction.