Leaf Powder Research Articles

Antibacterial and antioxidant activities of Alcea kurdica flower, leaf and root aqueous and organic extracts

Global concerns are rising due to complications associated with the use of chemical agents and antibiotic resistance. Consequently, research focus has shifted towards the quest for effective agents of biological origin. The aim of the present study was to assess the antioxidant and antimicrobial potentials of aqueous and organic extracts derived from various parts of Alcea kurdica. Different parts of A. kurdica were obtained and prepared into leaf, flower and root powders. The powders were extracted with aqueous and organic solvents. The antimicrobial activity of these extracts was assessed against bacterial pathogens using the agar well-diffusion assay. Additionally, the antioxidant effects of the extracts were evaluated using the DPPH (2,2-diphenyl-1-picrylhydrazyl) and resazurin dye scavenging assays. The results showed dose-dependent antibacterial activity against Staphylococcus aureus and Escherichia coli for both the organic and aqueous leaf and floral extracts. Furthermore, an antioxidant effect (>80%) was also observed for the organic and water extracts of the flowers, leaves and roots of the plant at the highest concentration (500 µg/ml), as compared to ascorbic acid, which served as the positive control using both the DPPH and resazurin methods. The findings of this study highlighted that A. kurdica can be considered a rich source of potential antioxidant and antibacterial agents, warranting future investigation to identify its active ingredients.

Upholding Seed Quality with Mid-storage Invigoration Treatments in Rice (Oryza sativa L.)

The groundbreaking research investigated the impact of mid-storage invigoration treatments on seed quality parameters in rice. The experimental design used in the study was Completely Randomized Design with three replications. The experiment was conducted at the Regional Agricultural Research Station, Pattambi, Kerala Agricultural University. The objective of the study was to understand the effect of mid-storage invigoration treatments on seed storage under ambient conditions. An experiment was conducted to evaluate the effect of ten different treatments on the quality of seeds, specifically the germination percentage, seed vigour index I, electrical conductivity, and mean germination time. The treatments tested were control- untreated seeds, hydration and dehydration treatment 1l/kg, ascorbic acid 10g/kg, calcium chloride 10g/kg, albizzia leaf powder 2g/kg, neem cake 5g/kg, neem leaf powder 2g/kg, neem oil 2ml/kg, red chilli powder 1g/kg and vayambu leaf powder 2g/kg. The rice variety Jyothi was used. Results showed that all the mid-storage invigoration treatments significantly improved the germination rate of the treated seeds when compared to the control under ambient storage conditions. Neem oil when used showed the highest germination percentage after five months of storage. In the last month of storage, neem oil showed a significantly higher vigour index which was comparable to hydration dehydration treatment and red chilli powder. Germination percentage and seedling vigour index I are positively associated with seed quality. Electrical conductivity and mean germination time are negatively correlated with seed quality. The least electrical conductivity was recorded in hydration dehydration treatment. Neem oil and hydration dehydration treatment had low mean germination time. Based on the research conducted, neem oil, hydration-dehydration, and red chili powder are the most effective treatments for improving the longevity of seeds. These eco-friendly and cost-effective mid-storage invigoration seed treatments can help farmers enhance the quality of their seeds and extend their storage life.

Influence of Integrated Nematode Management Modules against Meloidogyne incognita Infecting Cucumber in Poly-house

Nematodes management in poly-house through a single practice is a very typical task in present agricultural scenario so, an integrated nematode management trial was conducted for the management of Meloidogyne incognita on cucumber in poly-house. In the investigation different combinations of physical, cultural and chemical treatments were applied for effective management of hidden enemy of cucumber, M. incognita. The combinations of hot water at 1 litre per poly bag, organic amendment (tea waste, tobacco churi, poultry manure, water hyacinth powder and lantana leaf powder at 20 g/plant) and carbofuran (0.25 g a.i./plant and 0.50 g a.i./plant) were applied. Standard (Hot water at 1 litre + Neem cake 50 g/plant + phorate at 0.5 g a.i./plant) and untreated checks were also maintained to compare experimental findings. The results of investigation shows that all the applied treatments significantly reduced nematode infection and increase plant growth over untreated check. Among all treatment combinations, Highest yield of cucumber (4.171 kg/plant) was obtained with integration of Hot water at 1 litre + Neem cake 50 g/plant + phorate at 0.5 g a.i./plant followed by Hot water + Tobacco churi + carbofuran at 0.5 g a.i./plant (3.924 kg/plant) and Hot water + Tea waste + carbofuran at 0.5 g a.i./plant (3.781 kg/plant) as compared to untreated check (1.092 kg/plant).

Influence of Mulberry Leaf Powder Additive on Chemical and Physical Characteristics of Wheat and Rice Flour Butter Cookies.

People of all age groups consume cookies every day. Consumers' preferences for cookies supplemented with functional plant raw materials have recently increased. Therefore, this research aimed to investigate the influence of a mulberry leaf additive on the proximate and mineral compositions, total phenolic and total chlorophyll content, antioxidant activity, and the hardness and color properties of butter cookies. Wheat and rice flour butter cookies were prepared by replacing the flour with mulberry leaf powder at 0, 4, 8, and 12% (w/w). The results revealed that the investigated chemical and physical characteristics of butter cookies depend on the flour used (rice or wheat) and the addition of mulberry leaf powder. Wheat and rice flour cookies with 12% mulberry leaf powder had the significantly highest contents of fiber (20.34 and 20.23%, respectively), ash (1.73 and 1.75%, respectively), K (170.22 and 160.22 mg 100 g-1, respectively), and Ca (170.45 and 160.68 mg 100 g-1, respectively). The rice flour cookies enriched with 12% leaf powder had the greatest amounts of total phenolics (1.48 mg 100 g-1), Zn (12.25 mg kg-1), Mn (6.28 mg kg-1), Cu (1.95 mg kg-1), and antioxidant activity (67.98%). However, the wheat cookies without mulberry leaf powder contained the most B (9.12 mg kg-1), while the no-added rice cookies contained the most Fe (14.30 mg kg-1). Replacing flour with leaf powder increased the cookies' hardness and decreased their lightness. In conclusion, enriching butter cookies with freeze-dried mulberry leaves can improve their nutritional value and antioxidant activity.

Green tea supplementation prevented oxidative stress, fibrosis, and myocardial damage in isoproterenol-induced Swiss albino mice

IntroductionOne of the main causes of death and morbidity in people with cardiovascular disorders is myocardial infarction. This study investigated the impact of green tea (GT) leaf powder on isoproterenol (ISO)-induced myocardial infarction in Swiss albino mice. Methods and materials4 to 5 weeks male Swiss albino mice were divided into four groups with 6 mice in every group: Control, ISO, Control + GT and ISO+ GT leaves powder. All of the mice were sacrificed at the end of the investigation. Heart organ and blood plasma samples were taken, and they were tested for oxidative stress indicators and several biochemical parameters. To investigate the formation of mononuclear cells and fibrosis in the heart histopathological staining of tissue sections was also carried out. ResultsIn this experiment, supplementation with green tea leaf powder in ISO-administered mice prevented the increased activity of the AST, ALT, and ALP enzymes. Additionally, three more tests were performed such as CK-MB, Creatinine, and Uric acid whose levels were increased by isoproterenol, and treatment of green tea reduced these elevated levels. Due to the supplementation of green tea leaf powder, ISO-administered mice had lower levels of lipid peroxidation product, nitric oxide, advanced protein oxidation product, and myeloperoxidase. Mice exposed to ISO had considerably higher levels of oxidative stress markers and lower levels of cellular antioxidants including catalase activity, SOD activity, and reduced glutathione concentration. Additionally, the heart of ISO-induced mice revealed substantial fibrosis and inflammatory cell infiltration. Furthermore, green tea leaf powder supplementation prevented inflammatory cell infiltration and fibrosis in ISO-administered mice. ConclusionIn conclusion, the findings imply that supplementing with green tea leaf powder might lower the risk of myocardial infarction in mice given ISO, potentially by reducing oxidative stress, inflammation, and fibrosis.

Minerals and dietary fibre source snack made from Moringa leaves enriched with ginger

The findings from the 2021 Indonesian Nutritional Status Study reveal a substantial 24.4% prevalence of child stunting, emphasizing the need for diverse strategies to achieve the desired target. One such strategy involves ensuring the nutritional well-being of expectant mothers to prevent stunting. Moringa leaves are a rich source of essential nutrients. Incorporating Moringa leaf powder into catfish flour-based snacks can boost their protein and mineral content while overcoming any undesirable odors, though this application is currently limited to crackers. This study aimed to create snack products using Moringa leaves, such as tea, brownies, and pudding, with the addition of ginger to enhance dietary fiber and mineral content for pregnant women. The top formulations identified through sensory assessment are F1 tea, F1 brownies, and F2 pudding. These specific tea, brownies, and pudding variations boast dietary fiber content exceeding 3 grams and meet or exceed 15% of the recommended daily intake levels for iron and zinc for pregnant women, as stipulated by the nutrition label reference. As a result, they can be classified as wholesome snacks rich in dietary fiber, iron, and zinc.

Steam explosion-assisted grinding improves the functional properties and antioxidant activity of Java tea-leaf powders (Clerodendranthus spicatus).

Java tea is widely consumed and has multiple health effects. This study established a steam explosion (SE) pretreatment method to prepare Java tea-leaf powders. The physicochemical, functional properties, phenolic extraction, and antioxidant activity of Java tea-leaf powders produced by simple and SE-assisted milling methods were investigated. In comparison with simple milling, SE pretreatment broke the cell wall effectively and reduced the particle size of Java tea-leaf powders. Steam explosion-treated powders showed higher values for sensory signals, bulk and tap density, and for the water solubility index. After SE treatment, the adsorption capacities to glucose, soybean oil, and cholesterol of leaf powders were increased by up to 55, 95, and 80% respectively. The extracts from SE-treated powders also showed higher total polyphenol content and antioxidant activity. Steam explosion treatment is helpful for the improvement of functional properties and antioxidant activity, which can benefit the development and application of Java tea-leaf powders. © 2024 Society of Chemical Industry.

Pengaruh Kemasan Simpan Beras yang diberi Serbuk Daun Kemangi (Ocimum sanctum L.) Terhadap Serangan Kutu Beras (Sitophilus oryzae L.)

South Kalimantan BPS data (2022) shows that there has been a decline in rice production of 79.28 thousand tons (11.65%) so it is necessary to have rice suppliers from outside South Kalimantan to meet the rice demand target. Sitophilus oryzae is the main pest during the shelf life of rice. Controlling rice aphids can be done in various ways, including using appropriate storage media and applying vegetable pesticides to plants that are believed to be able to control them, one of which is basil. This research uses the factorial RAL method with 2 factors, namely packaging (W) and dose (D). The doses given are 0g, 10g, 14g, 18g. The packaging used is in the form of plastic containers and plastic sacks. Obtained 8 combinations, each repeated 3 times. So a total of 24 experimental units. The results of statistical analysis show that the combination of 18g of basil leaf powder in plastic packaging is the best treatment in suppressing the S. oryzae population with a mortality rate of 60%. During 30 days of observation, no new population growth was found and showed the lowest level of rice damage of 1.0% compared to other treatments.

Effect of Feeding Moringa oleifera Leaf Powder on the Growth and Performance of Osmanabadi Kids

The study was conducted at Goat Research Project, Vasantrao Naik Marathwada Agricultural University, Parbhani. Eighteen Osmanabadi kids were selected and were allocated to three experimental groups based on concentrate feed replacement with Moringa oleifera Leaf Powder (MOLP) i.e., T0 (0%), T1(10%) and T2 (20%). The study lasted for 90 days in intensive rearing system. The averages for weekly body weight (Kg) of kids fed with MOLP for group T0, T1 and T2 at the end of 13th week were 14.40±0.36, 14.86±0.47 and 16.40±0.45 kg, respectively depicting significantly higher values for T2 group than Ti group whereas non-significant difference between T0 and T1 group. The weekly body weight gain was 0.39±0.01, 0.40±0.01 and 0.54± 0.01 kg, respectively while the daily weight gain observed were 55.95±2.07, 56.90±1.31 and 76.64±1.67 gm respectively with highly significant difference between T2 group as compared to T1 and T0 group. The mean values for weekly and daily feed intake for groups T0, T1 and T2 were 70.95±0.43 and 10.14±0.06, 71.38±0.41 and 10.20±0.06, and 70.40±0.06, and 10.06±0.01 kg, respectively. The feed conversion efficiency (FCR) was significantly (P<0.01) better in T2 (6.49±0.00) as compared to T1 (8.82±0.03) and T0 (8.93±0.04). There was higher growth performance of Osmanabadi kids in T2 treatment group as compared to T1 and T0 groups. It can be concluded that the concentrate feed can be replaced up to 20% by Moringa oleifera leaf powder in Osmanabadi kids reared intensively without any adverse effect on growth.

The Effect of Japanese Papaya Leaf Powder (Cnidoscolus aconitifolius) as a Natural Preservative of Block Fish During Cold Temperature Storage

Mackerel (Rastelliger Sp.) is a small pelagic fish commonly consumed as a source of animal protein. Due to its high susceptibility to spoilage, effective storage and the use of natural preservatives are essential to extend its shelf life. Japanese papaya leaf powder, which contains alkaloids, flavonoids, tannins, and saponins, has potential as a natural preservative due to its antimicrobial properties. This study aims to evaluate the optimal combination of Japanese papaya leaf powder concentration and storage duration on the quality of mackerel stored at low temperatures. A completely randomized factorial design was employed, with two factors: the concentration of Japanese papaya leaf powder (0%, 6%, 8%, and 10%) and storage duration (0, 2, 4, and 6 days). Data were analyzed using ANOVA at a 5% significance level, followed by Duncan's Multiple Range Test (DMRT) at the same significance level. The results indicated that the best treatment combination was 10% Japanese papaya leaf powder and 4 days of storage. This combination yielded a moisture content of 64.51%, a total microbial count of 6.6 cfu/g, a total volatile basic nitrogen (TVBN) level of 23.28 mg/100g, a pH of 5.34, a trimethylamine (TMA) level of 6.15 mg/100g, and a water holding capacity (WHC) of 53.11%. The organoleptic assessment showed that the mackerel maintained acceptable color and aroma, indicating effective preservation.

Removal of COD, BOD and color of different industrial effluents using activated neem char

Characterization of industrial effluent i.e. sugar, dye, paper and pulp; and combined effluent is performed using the standard method, which indicates higher amount of pollutants exist in all the effluents. Adsorptive batch treatment is conducted using activated neem leaf powder (a-NLP), in which adsorbent dose, temperature and contact duration are the factors selected for this study to reduction of COD, BOD and color from individual and combined wastewater. Maximum elimination of COD, BOD and color is attained upto 93.4-82.4, 82.6-75.9 and 93.4-86.1 % respectively from sugar, dye, paper-pulp and combined effluent respectively at dosage of 23 gm/L a-NLP, temperature of 300 K and contact duration of 60 min. Adsorption isotherms such as Freundlich, Langmuir, Dubinin-Raduskevich, Flory-Huggins and Temkin Isotherm are examined in order to explore the adsorption mechanism. The reaction rate is estimated by different kinetic models (Pseudo First-, Pseudo Second-order, Intra-particle and Elovic equation). From correction coefficient value, Freundlich isotherm and Pseudo First-order govern adsorption process. Different thermodynamic parameters such as free energy of adsorption (∆G°), enthalpy change (∆H°) and entropy change (∆S°) are also accessed, which indicates that adsorption is thermodynamically feasible. Solvent desorption is carried out to regenerate this adsorbent for three consecutive cycles.

Efficacy of Eucheuma denticulatum Extract and Azadirachta indica Powder against Maize Weevil (Sitophilus zeamais) Infesting Stored Maize Grains

Storage insect pests destroy food grains in storage. In the present study, the insecticidal activity of red seaweed extract (Eucheuma denticulatum) and neem (Azadirachta indica) was examined against maize weevils (Sitophilus zeamais) and the quality of the stored maize was assessed for a three months storage period. Three rates (0.0, 1.6 and 4.8% w/w) of neem powder and seaweed extract were used as treatments. The collected data were subjected to analysis of variance (ANOVA) using General Linear Model in SPSS computer software packages (version 25) and mean comparisons were conducted using Turkey’s (HSD) test at the 5% level of significance. Sitophilus zeamais, mortality, grain weight loss, and rate of germination were recorded for each treatment. The 4.8%w/w mixture of seaweed extract and neem seed powder was more toxic, followed by the mixture of the extract and neem leaf powder (84.5% and 73.3% mortality rates, respectively), while in the control, mortality was 20%. In all treatments, Sitophilus zeamais mortality increased with increasing doses. Grain weight loss was high in the control (5.6%), followed by grains treated with 1.6% extracts of Eucheuma denticulatum alone (2.8%). The percentage of damaged maize grains was high in the control (14%) and low in grains treated with 4.8% seaweed extract and neem leaf powder (3.3%). Percentage germination was high in grains treated with 4.8% and 1.6% seaweed extracts alone (96% and 87% respectively) and in control (91%). Additionally, sensory evaluation results in terms of color, odor and overall acceptability showed that maize grains treated with both botanicals could be accepted by the general public for consumption. The results indicate promising potential for seaweed extract and neem powder in controlling maize weevils, offering sustainable and environmentally friendly solutions for stored grain protection.

Biological control of Sitophilus oryzae Management with Azardichta indica in Stored Maize Grains

This study was an effort to measure the efficacy about application of leaf powder of Azardichta indica as water-based paste between the layers of double layered storage bags and acetonic leaf extracts on single layered bags against Sitophilus oryzae to protect stored maize for a period of five months. The untreated double and single layered bags were set as controls. The we evil population, percent grain damage and percent weight loss after 5 months of storage was significantly lower in A. indica treated double layered bags with 2.0% w/w. However, untreated single layered bags recorded high population build up and maximum percent grains weight loss. The present work suggests that A. indica treated double bags provide better protection to maize from S. oryzae infestation up to five months and can be readily used as an alternative to synthetic pesticide des at the farm level.

Effects of postharvest management on the nutrient content of potato (Solanum tuberosum) tubers during storage

An experiment was performed to assess the effect of two plant powders and six storage methods on the proximate composition of potato tubers stored under ambient conditions to find a simple technology for storing potato tubers with a minimal nutrient degradation to reduce postharvest losses by smallholder farmers. Two plant powders and six storage methods were used in the study, which was designed as a 3 x 6 factorial experiment laid out in a completely randomized design (CRD) and replicated three times. The application of neem leaf powder to potato tubers and storage in the pit significantly (P ≤ 0.05) maintained protein (5.65%), fat (0.91%), fiber (0.75%), ash (2.70%), dry matter (30.63%), moisture (69.34%), and carbohydrate (20.62%) contents. Similarly, the pit storage method has influenced the nutrient content significantly (P ≤ 0.05) and is the best method for retaining protein (5.74%), fiber (0.78%), ash (2.81%), and moisture (69.83%) contents of potato tubers during storage under ambient conditions, and there is no significant (P > 0.05) interaction effect between the plant powder and the storage method. The study has found that the neem leaf powder and the pit storage significantly preserved the nutrient content of potato tubers during the four weeks of storage, which helped minimize postharvest losses. Keywords: basket, carton, floor, ginger, jute bag, neem, pit, polypropylene bag

Formulation & Evaluation of Aqueous Guava & Babul Leaves for the Treatment of Mouth Ulcer

This study delved the development and evaluation of a new herbal gel for mouth ulcers using pulverized guava leaves. The gel incorporated colorful attention of the leaves with Carbopol 934 and Propylene glycol as a base. Evaluation included assessments of the gel's physical parcels and natural exertion. Infrared spectroscopy verified no adverse commerce between the leaves and the gelatinizing agent. The performing gel was transparent, invariant, and had a neutral pH range( 7- 7.5). also, it demonstrated good rheological parcels, spreading and banishing fluently. Anti- fungal tests revealed significant effectiveness against Candida albicans and Aspergillus aureus. In vitro analysis verified the presence of flavonoids in the guava leaves, contributing to their potent antioxidant exertion. This suggests implicit for combating oxidative stress associated with mouth ulcers. The formulated gel offered a stable, safe, and potentially superior volition to conventional synthetic treatments for mouth ulcers. Its overall parcels suggest a promising advancement in this area. In conclusion, the herbal gel displayed a range of desirable characteristics- translucency, unity, good rheology, potentanti-fungal exertion, and significant antioxidant parcels. These findings place it as a potentially safe and effective herbal remedy for mouth ulcers, potentially outperforming being synthetic options. Keywords- Guava Leaves Powder, Gel, Mouth Ulcer, antioxidant, mucosal layer.

Flour Functionality, Nutritional Composition, and In Vitro Protein Digestibility of Wheat Cookies Enriched with Decolourised Moringa oleifera Leaf Powder.

This study investigated the potential of decolourised Moringa oleifera leaf powder (D-MOLP) in cookies to meet consumer demand for healthier food options, addressing the issue of low acceptability due to its green colour. D-MOLP and its non-decolourised counterpart (ND-MOLP) were incorporated into wheat flour to produce cookies. The results showed that neither decolourisation nor addition level (2.5 or 7.5%) significantly affected water activity or flour functionality, though slight differences in cookie colour were observed. The Moringa-enriched cookies exhibited an improved spread ratio as well as higher protein, phenolic content, antioxidant activity, and in vitro protein digestibility compared to control cookies. The detected phenolic acids included chlorogenic, ferulic, and fumaric acids, with the D-MOLP cookies showing superior nutritional properties, likely due to nutrient concentration and reduced antinutrients. Notably, glutamic acid was the major amino acid in all the cookies, but only lysine significantly increased across the cookie types. This suggests D-MOLP could be a promising alternative for food enrichment. Future research should address the consumer acceptability, volatile components, and shelf-life of D-MOLP-enriched cookies.

Effects of Mango leaf powder on reproductive hormones, oxidative stress markers, toxicity indicators, growth rate and carcass characteristics of guinea pig (Cavia porcellus)

Effects of Mango leaf powder on reproductive hormones, oxidative stress markers, toxicity indicators, growth rate and carcass characteristics of guinea pig (Cavia porcellus)

Influence of growth stages on the nutritional value of Momordica balsamina leaf powder

The nutritive mineral element concentrations of most African leafy vegetables (ALVs) are influenced by the plant’s growth stages. Momordica balsamina is an ALV in the Cucurbitaceae family, rich with nutraceutical and pharmaceutical properties, available in the plant at different growth stages. A greenhouse study was conducted to determine the responses of the nutritional value of M. balsamina leafy vegetable harvested at different growth stages. Six growth stages, namely, vegetative (reference), bud development, flower initiation, fruit set, fruit development and physiological maturity, served as treatments, with 10 replicates and arranged in RCBD. Treatments had highly significant (P ≤ 0.01) effect on the tested nutritional quality, namely, potassium (K), calcium (Ca), magnesium (Mg), zinc (Zn), iron (Fe) and phosphorus (P). Relative to the reference, K was the highest (1206 mg/L) at flower initiation stage, whereas the highest Ca (127.70 mg/L) and Zn (2.21 mg/L) concentrations occurred at the fruit development stage. At the physiological maturity stage, the highest Mg (25.19 mg/L), P (16.54 mg/L) and Fe (5.49 mg/L) concentrations, were recorded. Noticeably, during early growth stages, P, Fe and Zn were negatively reduced, when compared to K, Ca and Mg, which were increasing at every growth stages. In conclusion, the greatest accumulation of the tested mineral elements was mainly observed as from when the leafy vegetable started setting fruit until physiological maturity stage.

Preparation of novel green adsorbent (Tabernaemontana divaricata leaf powder) and evaluation of its dye (malachite green) removal capacity, mechanism, kinetics, and phytotoxicity

In this study, a novel green adsorbent, Tabernaemontana divaricata leaf powder (TD), was prepared, and its efficacy in removing malachite green (MG) dye from water, along with the associated mechanism and kinetics, was systematically evaluated for the first time. Characterization of TD was carried out using scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR). Optimization of MG dye removal was conducted by varying parameters such as pH, initial dye concentration, contact time, and TD dosage. Results demonstrated that TD exhibited a high adsorption capacity for MG dye (5.2131 mg.g−1), achieving a maximum removal efficiency of 89.5 % under optimized conditions: pH 7.0, initial dye concentration of 20 ppm, contact time of 120 min, TD dosage of 4 g/L, and temperature of 28.1 °C. Kinetic and isotherm models were applied to analyze the experimental data, revealing that the adsorption process most accurately followed Ho's pseudo-second-order kinetic model (R2 = 0.999). The high heats of adsorption observed in the isotherm study suggest prominent electrostatic interactions between adsorbate molecules and the surface, governing the chemisorption mechanism that dominates at the solid-liquid interface. This study underscores the potential of Tabernaemontana divaricata (jasmine) leaf powder as a cost-effective, environmentally friendly, and efficient adsorbent for the remediation of MG dye-contaminated water.

Valorization of Ginkgo biloba Leaf Powder as a Substrate in King Oyster Mushroom (Pleurotus eryngii) Cultivation.

Ginkgo biloba is widely planted as a colorful foliage tree, and its leaf can be used as a biomass energy source, but it has been underutilized for a long time. The aim of this study was to investigate the potential of garden waste as a substrate component in the cultivation process of the king oyster mushroom (Pleurotus eryngii), with the goal of enhancing both the yield of P. eryngii and the efficiency of energy use. The percentages of G. biloba leaf powder in the substrate were 10.5% and 21% to replace sawdust or sugarcane bagasse in a typical substrate. A substrate formulation that could completely replace sawdust and sugarcane bagasse was selected by analyzing mycelial growth rate, days of production, fruiting body length, biological efficiency, yield, stipe thickness, pileus diameter and laccase activity. The results showed that Y1 (treatment with 21% G. biloba leaf powder and sugarcane bagasse) had the highest yield (303.1 ± 31.9 g), which was higher than that of CK (control) (259.3 ± 37.4 g). The crude fiber content of the samples grown on substrate Y1 (as 7.43%) was higher than CK (7.37%). In addition, P. eryngii grown on substrate Y1 had the highest laccase activity for the complete colonization of the mycelium. Thus, these findings suggest that G. biloba leaf powder represents a viable and economical supplement for enhancing both the yield and quality of P. eryngii.