Methanolic Leaf Extract Research Articles

Comparative assessment of Rhazya stricta and Azadirachta indica methanolic leaf extracts for their antimicrobial efficacy against the selected foodborne pathogens

Comparative assessment of Rhazya stricta and Azadirachta indica methanolic leaf extracts for their antimicrobial efficacy against the selected foodborne pathogens

The Potential Alternative Antibacterial Activity of Falcata (Falcataria Falcata) Leaf Methanolic Extract against Staphylococcus Aureus and Escherichia Coli

The Potential Alternative Antibacterial Activity Of Falcata (Falcataria falcata) Leaf Methanolic Extract Against Staphylococcus aureus And Escherichia coli  Laliefe B. Arnan; Katherine Joy S. Escueta; Nor Rashida P. Rashid; Elyca M. Tacbobo; Queenie Rose M. Tinoy  Degree: Bachelor of Science in Pharmacy  Thesis Adviser: Junnin Gay L. Garay, RPh, CPh, MS Pharm Falcata is a plant that can be found in the Philippines and is used for the production of wood veneer and plywood. While in Indonesia, it is used as traditional remedy for malaria (Budiarti et al. 2020). They belong to the Fabaceae family, a family known for having great antibacterial effects (Gamo et al. 2015). This study used a percolation extraction method and the percentage yield is calculated to determine the yield from the falcata extract. Disc diffusion method is used for susceptibility testing and determining the zone of inhibition for the different groups. The CLSI guidelines for Staphylococcus aureus and Escherichia coli will be used to determine the antibacterial effect of the extract, in terms of resistance, intermediate, and susceptible results. From the results, the percentage yield of the methanolic crude leaf extract of Falcataria falcata is 2.67%. Leaves from the Falcataria falcata plant were extracted and tested against bacteria. The extracts showed promise in inhibiting the growth of Staphylococcus aureus and Escherichia coli bacteria, with 75% concentration as being more effective. However, these bacteria showed some resistance to all extract concentrations: S. aureus (90% - resistant, 75% - intermediate, 50% - resistant, 25% - resistant); E. coli (90% - resistant, 75% - resistant, 50% - resistant, 25% - resistant). Further study is needed to determine the exact antibacterial properties of the plant.

Anthelmintic Activity of Alcoholic Leaf Extract of Tectona grandis

Background: Helminthiasis is a major concern in the livestock sector due to the immense afflictions in the production and economy of the farmer. Chemical anthelmintics are used to combat them and parasites are acquiring resistance against these drugs. Hence, the development of new drugs or alternatives is the need of the hour for combating such infestations. This study was undertaken to determine the effect of methanolic leaf extract of Tectona grandis on strongyles. Materials and Methods: The dried leaves of Tectona grandis were subjected to Soxhlet extraction using methanol, and the extract was tested for its anthelmintic activity in vitro using egg hatch assay and larval motility assay. A dose of 250, 125, 62.5, 31.25, 15.625, and 7.8125 mg/ mL was used for the study. Fresh strongyle ova were exposed to different concentrations of the extract and observed after 48 hours for the hatch. L3 larvae obtained from coproculture were subjected to treatment with extract at different concentrations, and mortality per unit time was calculated. Fourier transform infrared (FTIR) spectroscopic analysis and Gas chromatography-mass spectrometry (GC/MS) were performed to identify the chemical nature of the extract. Results: There was a dose-dependent inhibition of hatch and larval mortality with a maximum inhibition of hatch at 250 mg/mL. There was a cent percent hatch in control wells and no loss of progressive motility in the larval motility test. On exposure to the extract, the larvae progressively lost their motility, and finally, there were caesurae of movement which indicated their death. The extract at 250 mg/mL killed all the larvae by 30 min, whereas, at 31.25 mg/mL, the mortality was 66.6% after 2 hr. Conclusion: The results suggest that methanolic extract from Tectona grandis leaves has a promising anthelmintic property and further studies are required for the isolation of active molecules.

Chemical Constituents of Borassus Aethiopum Methanol Leaf and Fruit Extract

Chemical Constituents of Borassus Aethiopum Methanol Leaf and Fruit Extract

UJI KONSENTRASI EKSTRAK DAUN SIRIH HUTAN BERPELARUT ORGANIK TERHADAP WERENG COKLAT

Rice is one of the priority food crop in Indonesia. The main problem faced by rice cultivation is the attack of brown planthopper (Nilaparvata lugens Stall.), this pest is one of the most important pest in rice cultivation, because it can cause a production decrease up to 100% by causing hopperburn symptoms and becoma a desease vector. Botanical pesticide of spiked pepper (Piper aduncum L.) can be the safe alternative to controling brown planthopper. This research aims to obtain effective concentration to controling brown planthopper. The research was conducted at The Plant Pest Laboratory Faculty of Agriculture and Organic Chemistry and Natural Materials Laboratory Faculty of Engineering, Riau University, from November 2020 to Januari 2021, using completely randomized design (CRD) consist of 6 treatments 4 replications. The treatment application consisted of concentrations of 0%, 0.20%, 0.40%, 0.60%, 0.80%, and 100% spiked pepper leaf methanol solvent extract. The result showed that different concentration significantly affected to the death of brown planthopper. Concentrations starting from 0.20% has good performance in killing pest, but the effective concentration to control brown planthoppers is 0.60% concentration of spiked pepper leaf methanol extract, with a total mortality of 82.50%, initial time of death 3.25 hours, and 50% time of death at 10 hours after application.

Bio Evaluation of Mirabilis Jalapa Leaves Extract for Anxiolytic Activity in Animal Model

Anxiety is an exaggerated feeling of apprehension, uncertainty and fear. It is an unpleasant state of tension with an anticipation of imminent danger. It may be regarded as a particular form of behavioral inhibition that occurs in response to environmental events that are Novel. In recent times, there are many indigenous plants have beneficial properties to treat mental disease and psychic complaints. Mirabilis Jalapa Linn (family- Nyctanginaceae) is one of the plants that used for health care and medicinal purpose for thousands of years. It has traditionally been used in the treatment of gastrointestinal disorders, muscle pain, abdominal colic, and diarrhea. However, studies have evidenced its antibacterial, antiviral, antioxidant, anti-diabetic, anti-inflammatory, anti-stress and many other activities. The purpose of this study was to prepare & evaluate Methanolic extract of leaves of mirabilis Jalapa for the treatment of anxiety in animal models. The dried leaves were extracted with methanol separately and administered the dose of 200mg/kg and employed in elevated plus maze test and open field test with 2mg/kg i.p.of Diazepam as a standard drug to assess the anxiolytic activity. The result reveled that methanol leaf extract was more impetus due to high amount of flavonoid, phenolic compound, steroids; terpenoid contents possess tremendous anti-anxiety activity. This study suggested that the M.Jalapa is much more active compound consistent medicinal plant to derive a potent drug against Anxiety

Quantitative Phytochemical Investigation, Antibacterial Potency, and Drug-ability Assessment of Three Indian Medicinal Plants Leaf Extracts Using Bioinformatics Tools

Natural regimens have long-held ethnomedicinal values, serving as primary sources for mainstream medicine. Therefore, scientists are paying more attention to studying the biological activity of existing plant species in organized ways to select potent bioactive metabolites to use for specific therapeutic purposes. This study used the same approach to find potent antibacterial phytoconstituents in three well-known Indian medicinal plants: Psidium guajava L., Syzygium cumini L. and Punica granatum L. In the earlier study, methanolic leaf extracts of the above plant extracts were more effective than n-hexane extracts against biofilm and drug-resistant pathogenic bacteria. Accordingly, we selected methanolic crude extracts for gas chromatography-mass spectrometry (GC-MS) to identify the phytoconstituents presented. In addition, we added a few reported candidates from the above plant extracts for molecular docking studies against four bacterial targets. For molecular docking studies, we retrieved all phytoconstituents or ligands from the PubChem database and bacterial target proteins from the protein data bank using PyRx 0.8-AutoDock 4.2 software. Furthermore, we used various bioinformatics and chemoinformatics tools to examine the investigated phytoconstituents physicochemical properties, toxicity, and drug-ability profiles. Out of the 30 GC-MS report-derived candidates from three plants, P5 from P. guajava, P18 from S. cumini, and P21 from P. granatum had the potent binding ability with bacterial targets. In the same way, out of the 30 reported candidates, P39, P43, and P56 from three plants, along with amikacin, showed strong binding against the same bacterial target. Both sets of candidates showed favorable physicochemical and toxicity profiles; however, all GC-MS-derived and few reported candidates exhibited negative drug-likeness. The study reveals that these crude extracts have antibacterial properties because they contain both GC-MS and existing phytoconstituents. The study starts with a crude extraction and then uses bioinformatics to choose two possible antibacterial candidates, ursolic acid and punicacortein A. This platform could be useful for finding an antibacterial agent that works specifically on a specific target. To sum up, the study encourages the isolation of more bioactive candidates from different Indian medicinal plants and uses bioinformatics tools to speed up the selection of strong leads that can speed up the process of making antibacterial drugs within limited resources.

Assessment and comparison of phytochemical constituents and biological activities of endemic Odonterrhena floribunda (Brassicaceae) from Türkiye

ABSTRACT This study aimed to evaluate the phenolic profile, antioxidant, antibacterial, and antidiabetic properties of ethanolic and methanolic extracts of the different parts of the endemic Odontarrhena floribunda. The antioxidant activities of O. floribunda were determined with 1,1-diphenyl-1-picrylhydrazyl (DPPH) and the copper-reducing antioxidant capacity (CUPRAC) assays. The strongest antioxidant activity was recorded in methanolic root extract (IC50:1.68±0.25 μg/mL) for DPPH and ethanolic root (λ450:0.68±0.20) extract for CUPRAC. Antibacterial activities of the extracts were displayed by the Resazurin Microplate Method (REMA). All plant extracts analyzed demonstrated stronger effects on Acinetobacter baumannii (ATCC 02026) than those of the standard compound. The in vitro α-amylase and α-glucosidase inhibitory of the plant extract were assessed using the Iodine method. Except for the methanol leaf extract, all other extracts showed strong α-glucosidase inhibitory activity (0.88–2.13 mmolTE/g). These results show that this endemic O. floribunda is a potential natural antioxidant, antibacterial, and enzyme inhibitor source.

Total Phenolics, Flavonoids, and ɑ-Glucosidase Inhibitory Activity of Red Betel (Piper crocatum) Extract in Various Solvents

Alpha-glucosidase enzyme activity needs to be inhibited to avoid increasing blood sugar levels in diabetics. Red betel leaf extract is reported to have active compounds such as phenolics and flavonoids that have alpha-glucosidase enzyme inhibition activity. Previous research showed that 70% betel leaf extract has an ɑ-glucosidase enzyme inhibition activity of 69.84%, but it is not yet known how the effect of extracts with different levels of polarity on the inhibition activity of alpha-glucosidase enzyme. This study aimed to determine extracts that have optimal levels of phenolic and flavonoid content and the highest suppression of α-glucosidase activity. The multistage maceration method was used to extract red betel leaf simplicia using solvents with different levels of polarity (n-hexane, ethyl acetate, methanol). Tests were conducted on overall phenolic concentration, flavonoids, and ɑ-glucosidase inhibition ability. Data were analyzed using one-way ANOVA test with Tukey's further test (p<0.05). The results of the assessment of phenolic concentration, flavonoids, and α-glucosidase inhibitory activity as a whole showed statistically significant differences (p<0.05) in all extracts with the highest total phenolic and flavonoid content found in the n-hexane extract of 25.68 ± 0.29 mg GAE/g and 65.84 ± 0.96 mg QE/g respectively. Meanwhile, methanol extract of red betel leaf became the best extract in this study with the greatest inhibition among all extracts with a percent α-glucosidase inhibition value of 43.87 ± 1.83%.

Preliminary Screening of Phytochemicals and Antibacterial Activity of Medicinal Plant Ber (<i>Ziziphus mauritiana</i> Lamark)

The most important genus in the Rhamnaceae family is Ber (Ziziphus mauritiana Lamk). Phytochemicals present in Ziziphus have antioxidant as well as antimicrobial properties. The present study aimed to detect various phytochemicals and evaluate the antibacterial activity in the Z. mauritiana leaf extracts. Leaves from 14 cultivars/germplasm/varieties of Z. mauritiana were used to explore the presence of various phytoconstituents such as alkaloids, flavonoids, cardiac glycosides, phenols, saponins, protein, steroids, tannins, lignins, and reducing sugar. These phytoconstituents have antioxidant, antibacterial, and hepatoprotective activities. The preliminary antimicrobial activity screening of Ziziphus leaf extract against bacterial strains-Bacillus subtilis (Gram-positive), Staphylococcus aureus (Gram-positive), Salmonella abony (Gram-negative), E. coli DH5α (Gram-negative) were performed by using well diffusion method. The results of various phytochemicals like alkaloids, phenolics, flavonoids, tannins, carbohydrates, proteins, lipids, and amino acids were reported in all selected commercial varieties/cultivars/ germplasm. On the other hand, saponins were found completely absent in methanolic solvents of all varieties/cultivars. The aqueous and methanolic solvent leaf extract were tested for antibacterial activity at different concentrations (25mg/ml, 50mg/ml, and 100mg/ml) against the bacterial strains of B. subtilis, S. aureus, S. abony, and E. coli DH5α, respectively. This study showed that the phytochemicals extracted from Z. mauritiana leaves have potential antimicrobial activities.

Effects of solvent extraction on the phytoconstituents and in vitro antioxidant activity properties of leaf extracts of the two selected medicinal plants from Malawi

This study evaluated and compared the phytochemical and antioxidant properties of the solvent extracts of Azadirachta indica A. Juss and Vernonia amygdalina Del leaves. Methanolic and aqueous extracts showed high (P ≤ 0.05) extract yields (in %), compared to chloroform and ethyl acetate extracts from both V. amygdalina and A. indica leaves. The study exhibited high phytochemical content in methanol and aqueous extracts compared to chloroform and ethyl acetate extracts, confirming the potential for medicinal use. V. amygdalina methanol and aqueous extracts had higher (P ≤ 0.05) total phenolic content (TPC), in mg GAE/gDW, (158.810±0.846 and 217.883±0.265, respectively) than chloroform (37.574±0.118) and ethyl acetate (104.758±0.236) but higher ethyl acetate content in A. indica extracts. Low polar solvents extracted high (P ≤ 0.05) total flavonoids, in mgQE/gDW, (367.051±0.858 and 149.808±0.009) compared to high polar solvents (14.863±0.071 and 54.226±0.014 ) in V. amygdalina while as in A. indica leaf extracts, low polar solvents showed high TFC ( 658.469±3.451 and 275.288±10.490) compared to high polar solvents (26.312±0.063 and 48.858±0.063) respectively. In vitro total antioxidant capacity, in mg/g, was higher in polar solvents than in low-polar solvents, ranging from 34.300±1.784 to 121.015±6.839 for A. indica ethyl acetate and methanolic extracts. A strong correlation between TPC and tannic acid content was observed, except in A. indica methanolic extracts of A. indica. Ferric reducing power was high, except for V. amygdalina chloroform and methanol leaf extracts, which were lower (P≤ 0.05) than that of the standard ascorbic acid. The study revealed that high polar solvents, such as methanol and water, are more efficient in the extraction of antioxidants from A. indica but lower in V. amygdalina extracts. High phytochemical content and antioxidative capacity could be significant in treating various diseases in humans.

Larvicidal Activity of Melissa officinalis and Rosmarinus officinalis Extracts and Their Lethal Impact on Detoxifying Enzymes in Aedes aegypti L. Larvae

Background & Objectives: Mosquito-borne diseases significantly impact global health, particularly in tropical regions. While synthetic insecticides are currently employed to control mosquito vectors, their detrimental effects on ecosystems and persistence necessitate alternative control methods. Botanicals, owing to their diverse phytocompounds, offer potential for controlling and preventing vector-borne diseases by targeting insect eggs and larvae. This study aimed to evaluate the toxicity of Melissa officinalis and Rosmarinus officinalis extracts (methanolic and aqueous) against Aedes aegypti larvae, a vector of arboviruses. Materials & Methods: Total protein content in control and plant extract-exposed larval homogenates was estimated using bovine serum albumin as a standard. Acetylcholinesterase (AChE) and carboxylesterase assays were performed to determine larvicidal effects. Larval mortality was assessed after 24 hours of exposure. Results: Our findings revealed that the methanolic leaf extract of M. officinalis exhibited superior larvicidal activity (100% at 1000 ppm) compared to the methanolic R. officinalis extract (84±2.45% at 1000 ppm). In contrast, the aqueous extracts of both plant species inferred no larvicidal activity. The LC50 and LC90 values for M. officinalis methanolic extract were 378.7 ppm and 795.8 ppm, respectively, whereas those for R. officinalis were 648.9 ppm and 1152.9 ppm, respectively. Furthermore, biochemical assays measuring total protein, acetylcholinesterase, α-carboxylesterase, and β-carboxylesterase activities were conducted for M. officinalis, corroborating and substantiating its larvicidal properties. Conclusion: This study demonstrates that the methanolic leaf extract of M. officinalis possesses significant larvicidal efficacy against A. aegypti. These findings suggest that this plant or its phytocompounds could serve as a bioinsecticide, offering a potential alternative to environmentally toxic and non-biodegradable synthetic insecticides.

Ameliorative Efficacy of <i>Moringa oleifera</i> Lam. against the Sodium Fluoride Induced Nephrotoxicity in Wistar Rat

A study was conducted to evaluate the protective efficacy of a methanolic leaf extract of Moringa oleifera L. (MO) for the remediation of nephrotoxicity induced by exposure to a significant groundwater pollutant, fluoride. Wistar albino male rats weighing 150–200 g were divided into five groups, each containing five animals. The control group received normal drinking water, and Group I received sodium fluoride in drinking water (20 mg/l daily) for 90 days. Group II received M. oleifera extract (250 mg/l) for 90 days. Group III received sodium fluoride 20 mg/l along with a low dose of M. oleifera extract (250 mg/l) for 90 days, and Group IV received sodium fluoride 20 mg/l along with a high dose of M. oleifera extract (500 mg/l) for 90 days. After 90 days, the kidneys and blood samples were obtained to evaluate their histological characteristics and assess the levels of oxidative stress and various enzyme parameters. The current investigation showed that sodium fluoride has a notable impact on renal function by elevating levels of creatinine, Blood Urea Nitrogen (BUN), urea, as well as Serum Glutamic Oxaloacetic Transaminase (SGOT), Serum Glutamate Pyruvate Transaminase (SGPT), and Alkaline Phosphatase (ALP) indicators. Further exposure resulted in a decrease in Superoxide Dismutase (SOD) and Catalase (CAT) levels, whereas Lipid Peroxidation (LPO) activity increased in kidney tissue. The histopathological examination of the kidney revealed glomerular enlargement, degenerative changes in Bowman’s capsule, and alterations in the tubular portion of the glomerulus. The study’s findings indicate that the administration of M. oleifera extract had a notable impact on the renal function and antioxidant status of rats subjected to sodium fluoride treatment. The administration of M. oleifera extract demonstrated a significant ability to mitigate the nephrotoxic effects induced by sodium fluoride in rats, mainly when both substances were concurrently administered. M. oleifera extract may also provide hepatoprotective effects by modifying membrane permeability and blocking enzymes from entering the serum.

Chlorophyll deficiency in Agave angustifolia Haw.: unveiling the impact on secondary metabolite production.

The albino phenotype of Agave angustifolia Haw. accumulates higher levels of phenylalanine and phenylpropanoids, while the green phenotype has a greater concentration of phenolic compounds. The metabolic consequences of chlorophyll deficiency in plants continue to be a captivating field of research, especially in relation to production of metabolic compounds. This study conducts a thorough analysis of the metabolome in green (G), variegated (V), and albino (A) phenotypes of Agave angustifolia Haw. Specifically, it examines the differences in the accumulation of compounds related to the phenylpropanoid and flavonoid biosynthesis pathways. Methanol extracts of leaf and meristem tissues from the three phenotypes grown in vitro were analyzed using liquid chromatography coupled with quadrupole time-of-flight high-resolution mass spectrometry (UPLC-MS-QTOF) for untargeted metabolomics and triple quadrupole (QqQ) mass spectrometry for targeted metabolomic analyses. By employing these methods, we discovered notable differences in the levels of important metabolites such as L-phenylalanine, 4-hydroxyphenylpyruvic acid, and various flavonoids among the different phenotypes. The results of our study indicate that the A phenotype shows a significant increase in the levels of phenylalanine and phenylpropanoids in both leaf and meristem tissues. This is in contrast to a decrease in flavonoids, suggesting a metabolic reprogramming to compensate for the lack of chlorophyll. Significantly, compounds such as kaempferol-3-O-glucoside and rutin exhibited significant quantitative reduction in the A leaves, suggesting a subtle modification in the production of flavonols and potentially a changed mechanism for antioxidant protection. This study emphasizes the complex metabolic changes in A. angustifolia´s chlorophyll-deficient phenotypes, providing insight into the complex interplay between primary and secondary metabolism in response to chlorophyll deficiency. Our research not only enhances the comprehension of plant metabolism in albino phenotypes but also opens new avenues for exploring the biochemical and genetic basis of such adaptations, with potential biotechnological applications of these distinct plant variants.

Efficacy of medicinal plants and their derived biomolecules against Plasmodium falciparum

Many apicomplexan pathogens pose significant threats to humans and domestic animals, with the lack of effective drugs and drug resistance representing major challenges in disease management. To address this, the search for new and potent antimalarial drugs is crucial. Plant-based formulations offer a promising alternative for such drug development. Here, we evaluated the in vitro antiplasmodial activity of nine plant extracts, traditionally used to treat fever-like symptoms in Bangladesh. We assessed the antimalarial activity of plant extracts by using the Plasmodium falciparum 3D7 growth inhibition assay, an invasion assay, and a cytotoxicity assay. Of the nine plants studied, ethanolic and methanolic leaf extracts of Ficus hispida, Streblus asper, and Boerhavia repens exhibited high antiplasmodial activity, with IC50 values of 9.31, 4.13, 9.63 μg/ml (ethanolic) and 15.12, 6.63, 7.58 μg/ml (methanolic), respectively, and minimal toxicity (cell viability >80%). Clerodendrum viscosum displayed antiplasmodial effects with IC50 values of 28.90 μg/ml (ethanolic) and 30.57 μg/ml (methanolic). Adhatoda vasica, Mussaenda corymbosa, and Amaranthus spinosus ethanolic extracts showed antimalarial effects with IC50 values of 61.78 μg/ml, 66.31 μg/ml, and 64.14 μg/ml, respectively. However, methanolic extracts of A. vasica and A. spinosus had IC50 values >100 μg/ml. The ethanolic and methanolic extracts of A. vasica, A. spinosus, F. hispida, S. asper, and B. repens significantly reduced parasitemia by inhibiting invasion into erythrocytes. This study highlights the robust antimalarial activity and low cytotoxicity of leaf extracts of F. hispida, S. asper, and B. repens, indicating the presence of antimalarial compounds that warrant further investigation.

Photocatalytic, antioxidant, and antibacterial activities of biocompatible MgO flake-like nanostructures created using Piper betle leaf extract

In this study, biocompatible magnesium oxide (MgO) nanostructures were synthesized using an aqueous solution of Piper betle leaf methanolic extract. The color change during the synthesis process, i.e., colorless to dark brown solution, indicates the formation of MgO nano structures which is further confirmed through UV–Visible spectrum that exhibited characteristic absorption band at 329 nm. Results obtained from morphological analysis reveal that MgO nano flakes (MgO NFs) are present as triangular and polyhedral shapes having an average size ranging between 150 and 200 nm with clear lattice fringes. Secondary metabolites were clearly identified from the FTIR spectrum, and they helped in reduction and stabilization during the synthesis of MgO NFs. The XRD pattern displayed Braggs planes of (111), (220), (220), (311), and (222) with a face centered cubic (FCC) structure, which was further confirmed from the SAED pattern using HRTEM analysis. The as-synthesized MgO NFs manifested profound antimicrobial activity against gram positive as well as gram-negative bacteria with favorable biocompatibility. Furthermore, methylene orange dye was degraded almost completely (98.36 %) in the presence of MgO NFs photo catalysts within 1 h of sunlight illumination. In addition, MgO NFs displayed high level of antioxidant properties with recorded IC50 value of 15.89 μg/ml.

Impact of Irpex lenis and Schizophyllum commune endophytic fungi on Perilla frutescens: enhancing nutritional uptake, phytochemicals, and antioxidant potential

BackgroundEndophytic fungi (EF) reside within plants without causing harm and provide benefits such as enhancing nutrients and producing bioactive compounds, which improve the medicinal properties of host plants. Selecting plants with established medicinal properties for studying EF is important, as it allows a deeper understanding of their influence. Therefore, the study aimed to investigate the impact of EF after inoculating the medicinal plant Perilla frutescens, specifically focusing on their role in enhancing medicinal properties.ResultsIn the current study, the impact of two EF i.e., Irpex lenis and Schizophyllum commune isolated from A. bracteosa was observed on plant Perilla frutescens leaves after inoculation. Plants were divided into four groups i.e., group A: the control group, group B: inoculated with I. lenis; group C: inoculated with S. commune and group D: inoculated with both the EF. Inoculation impact of I. lenis showed an increase in the concentration of chlorophyll a (5.32 mg/g), chlorophyll b (4.46 mg/g), total chlorophyll content (9.78 mg/g), protein (68.517 ± 0.77 mg/g), carbohydrates (137.886 ± 13.71 mg/g), and crude fiber (3.333 ± 0.37%). Furthermore, the plants inoculated with I. lenis showed the highest concentrations of P (14605 mg/kg), Mg (4964.320 mg/kg), Ca (27389.400 mg/kg), and Mn (86.883 mg/kg). The results of the phytochemical analysis also indicated an increased content of total flavonoids (2.347 mg/g), phenols (3.086 mg/g), tannins (3.902 mg/g), and alkaloids (1.037 mg/g) in the leaf extract of P. frutescens inoculated with I. lenis. Thus, overall the best results of inoculation were observed in Group B i.e. inoculated with I. lenis. GC-MS analysis of methanol leaf extract showed ten bioactive constituents, including 9-Octadecenoic acid (Z)-, methyl ester, and hexadecanoic acid, methyl ester as major constituents found in all the groups of P. frutescens leaves. The phenol (gallic acid) and flavonoids (rutin, kaempferol, and quercetin) were also observed to increase after inoculation by HPTLC analysis. The enhancement in the phytochemical content was co-related with improved anti-oxidant potential which was analyzed by DPPH (% Inhibition: 83.45 µg/ml) and FRAP (2.980 µM Fe (II) equivalent) assay as compared with the control group.ConclusionInoculation with I. lenis significantly enhances the uptake of nutritional constituents, phytochemicals, and antioxidant properties in P. frutescens, suggesting its potential to boost the therapeutic properties of host plants.Graphical

Investigation into the Potential of Paullinia pinnata (Linn.) Methanol Leaf Extract against Toxicity by Ethylene Glycol Monomethyl Ether in Wistar Rats

Investigation into the Potential of Paullinia pinnata (Linn.) Methanol Leaf Extract against Toxicity by Ethylene Glycol Monomethyl Ether in Wistar Rats

Anti-Oxidant and Anti-Inflammatory Properties of Talinum triangulare Methanol Leaf Extract on Cadmium-Induced Cognitive Dysfunction in Male Wistar Rats

Anti-Oxidant and Anti-Inflammatory Properties of <i>Talinum triangulare</i> Methanol Leaf Extract on Cadmium-Induced Cognitive Dysfunction in Male Wistar Rats

GC-MS Fingerprinting of Methanolic Extract of Moringa oleifera Stem, Leaf and Root

GC-MS Fingerprinting of Methanolic Extract of Moringa oleifera Stem, Leaf and Root