Azadirachta Indica Leaf Extract Research Articles

Effect of Azadirachta indica against Sodium Benzoate Induced Hepatorenal Toxicity in Wistar Rats- An Experimental Interventional Study

Introduction: Azadirachta indica (Neem) is one of the widely used plants which has various medicinal properties like antipyretic, antimicrobial, antitumour, anti-inflammatory, antiulcer and antidiabetic effects. Silymarin-a milk thistle derivative has its own clinical significance. Sodium Benzoate (SB) is a versatile food preservative used in packaged food and drink industries which is consumed by the people unknowingly above World Health Organisation (WHO) standards which causes potential cytotoxicity. Aim: To evaluate the effect of Azadirachta indica leaf extract and the combination effect of Azadirachta indica leaf extract (Neem) with Silymarin against SB induced hepatorenal toxicity in adult male albino wistar rats. Materials and Methods: This experimental interventional animal study was conducted at Central Animal House at Aarupadai Veedu Medical College and Hospital, Puducherry, India from July 2021 to August 2021 for a period of 14 days. Total 30 male wistar rats were randomised into five groups with six rats into each group. The groups were Group 1 Control received only distilled water, Group 2 SB 200 mg/kg bw (SB) alone, Group 3 SB+Silymarin 100 mg/kg bw, Group 4 SB+ Azadirachta indica aqueous extract 400 mg/kg bw, Group 5 SB+Silymarin 100 mg/ kg bw+Azadirachta indica aqueous extract 400 mg/kg bw for 14 days through oral gavage. Doses and duration were determined based on previous studies. Blood was drawn from a retroorbital puncture, animals were sacrificed by euthanasia, a part of Liver and Kidney samples were sent for histopathological examination-cell structure, integrity, inflammation. Biochemical parameters of liver function tests-Serum Alanine Transaminase (ALT), Serum Aspartate aminotransferase (AST), Serum Alkaline Phosphatase (ALP) and Kidney function tests: urea, creatinine and uric acid were measured in serum. Statistical analysis were done by one-way Analysis of Variance (ANOVA) followed by Dunnet’s post-hoc test used for intergroup comparison, p-value <0.05 considered to be significant. Results: There was a significant increase in the activities of liver enzymes ALT, AST, ALP and kidney function (creatinine, uric acid) in the SB alone treated group when compared with the control group. Hepatorenal protection of Neem extract was shown by significant decrease in liver and renal parameters which was comparable to that of control and Silymarin standard drug. Combination of silymarin and neem showed significant protection in liver (ALT, AST, ALP) and kidney function (urea, creatinine, uric acid) when compared to neem alone treated group. All the results were substantiated by histopathological examination of liver and kidney tissues. Conclusion: This study suggests effect of Azadirachta indica leaf extract with Silymarin possess hepatorenoprotective effect against SB induced damage in rats. Combination effect of Azadirachta indica leaf extract with Silymarin significantly proved the hepatorenal protectivity when compared with Neem alone treated group.

Abstract 1415: Modulatory effect of Azadirachta indica leaf extract in mitigating isoproterenol induced cardiotoxicity

Abstract 1415: Modulatory effect of Azadirachta indica leaf extract in mitigating isoproterenol induced cardiotoxicity

Phytochemical-assisted Synthesis of Titania Nanoparticles using Azadirachta indica Leaf Extract as Photocatalyst in the Photodegradation of Methyl Orange

The biosynthesis procedure for nanomaterial preparation is a promising alternative due to its simplicity and environmental friendliness. In this work, TiO2 NPs were biosynthesized using the aqueous leaf extract of Azadirachta indica. The influence of the extract volumes, solvents, and acetic acid on the properties of TiO2 NPs was studied. Phytochemical screening and ATR-FTIR spectrum confirmed the presence of phenolic compounds in the leaf extract. XRD patterns showed that the samples were mainly in the anatase phase. However, for the water-based samples and when 1 and 2 mL of extract volumes were used, anatase/brookite mixture was observed. FESEM images displayed almost spherical and agglomerated NPs. UV-Vis-NIR studies showed that the samples’ bandgaps values are within the range of anatase TiO2. The photocatalytic activity of the TiO2 NPs was evaluated in the photodegradation of methyl orange (MO) under UV light irradiation. The water-based sample synthesized using 2 mL of the extract achieved 98.62% of MO degradation within 270 min and demonstrated the highest pseudo-first-order photodegradation kinetic constant of 0.0147 min-1. These results indicate that the use of the plant-based biosynthesis method with water as the solvent successfully produced TiO2 NPs with good physicochemical properties and photocatalytic activity in the photodegradation of organic dye. Copyright © 2022 by Authors, Published by BCREC Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0).

Green and Eco-Friendly Treatment of Textile Wastewater by Using Azadirachta indica Leaf Extract Combined with a Silver Nitrate Solution

The present study was conducted to treat textile industrial wastewater through the combination of green and synthetic solutions. Two case studies were applied for the treatment of wastewater. In the first case, discharged industrial effluent was reacted with Azadirachta Indica leaf extract solution for a 4 to 72 h retention time. After the reaction, some pollutants were treated but most required higher retention time and concentration of A. indica extract, which could be a potential adsorbent for wastewater treatment. In the second case, the discharged industrial effluent was reacted with A. indica solution with silver nitrate AgNO3 solution and was used as a treating agent for wastewater with a 4 to 72 h retention time. The second case was found to be better than the first case as it treated a greater number of pollutants. Moreover, treatment plant design feasibilities will be required for the application of findings of the present study on an industrial scale. This study can be useful to improve industrial estate’s environmental conditions for reducing pollution by industrial wastewater. There is also a need to raise environmental awareness regarding wastewater’s health effects in local communities.

Antibacterial and Anti-Quorum Sensing Studies of Extracellularly Synthesized Silver Nanoparticles from Azadirachta indica (Neem) Leaf Extract

Azadirachta indica (Neem ) is an important medicinal plant with proven strong antiseptic, antiviral, antifungal and antibacterial properties. The study here presents the antibacterial and QS (Quorum Sensing) inhibitory potential of biogenic Silver nanopaticles (AgNPs) from Azadirachta indica leaf extracts. The nanoparticles were synthesized using an aqueous extract of Azadirachta indica leaves and silver nitrate solution. The size, crystal structure, elemental composition and other physical properties of nanoparticles were determined using different microscopic and spectroscopic techniques. The average diameter of the nanoparticles was found to be between 20-43 nm with crystalline morphology. These extracellularly synthesized AgNPs strongly inhibited Gram negative pathogenic species and exhibited demonstrable anti- Quorum Sensing (QS) activity as evident from pigment inhibition and 75- 80% decrease in biofilm mass on AgNPs treatment in a dose dependent manner.

PHYTOCHEMICAL SCREENING AND GC-MS ANALYSIS OF MEDICINAL PLANTS AND THEIR GROWTH PROMOTING EFFICIENCY IN Cirrhinus mrigala – A COMPARATIVE STUDY

Aquaculture has a significant impact on wholesome human food. These species contain a wide range of nutrients, such as proteins, lipids, and a number of minerals. The environment, immunity, and growth rate of the fish must be improved with mediating elements in order to achieve mass production while preserving the stability of aquaculture. The use of herbal treatments to strengthen immune response has been discussed in numerous studies. This study has focused on the effects of a few plants leaf extract, including neem(Azadirachta indica), thulasi (Ocimumsanctum) and thuthi (Abutilon indicum) on Cirrhinus mrigalagrowth rate. By performing a preliminary phytochemical screening and GC-MSanalysis, the entire leaf extract was described. The extract from the leaves of the three therapeutic plants contains more than 30 significant phytocomponents. A feeding trial was done to see how Cirrhinus mrigala fingerlings responded to dietary leaf extracts of Azadirachta indica, Ocimum sanctum, Abutilon indicum, and combination extract. Given that herbs are a natural and risk-free strategy, adding such herbs to animal diets to enhance aquaculture appears helpful. A substantial increase in weight and growth rate was observed in all studies that examined growth rate metrics, such as specific growth rate and percent growth rate. This evidence supported the benefits of using specific herbs, particularly combined leaves extract, which has been shown to significantly promote growth in Cirrhinus mrigala.

EXPLORING the prophylactic potential of Azadirachta indica leaf extract against dyslipidemia

Ethnopharmacological relevanceSeveral studies revealed that different parts of Azadirachta indica A. Juss, has therapeutic potential against inflammatory issues and dyslipidemia which is a major contributing cause to cardiovascular diseases, oxidative stress and serum glucose levels, etc. Aim of studyPresent study was conducted to evaluate anti-dyslipidemic capacity of Azadirachta indica leaf extract in dyslipidemic rabbits. Materials and methodsEthanolic extract of Azadirachta indica leaves was obtained by using Soxhlet apparatus. This extract was used for efficacy study on rabbits. In this context, 25 healthy rabbits were selected for study, Efficacy trial involved five groups of rabbits, 5 rabbits in each group; NC (Negative Control); healthy rabbits received normal diet. In remaining 20 rabbits, dyslipidemia was induced by using high fat diet for 28 days followed by administration of Azadirachta indica leaf ethanolic extract for 60 days in a dose-dependent manner. PC (Positive Control) include dyslipidemic rabbits received normal diet while G1, G2, G3 groups included dyslipidemic rabbits receiving different concentrations of Azadirachta indica leaf extract (i.e. 300, 500 and 700 mg/kg of body weight, respectively). Blood samples were analyzed for serum lipid profile after every 15 days to determine the effect of treatments. ResultsSignificant reduction in total cholesterol (60 ± 3.4 mg/dL), triglycerides (40.31 ± 2.5 mg/dL) and low-density lipoprotein (28.87 ± 2.1 mg/dL) was observed in G2 (P ≤ 0.05)while a significant increase was observed in high-density lipoprotein (60.47 ± 1.7 mg/dL) of G2 (P ≤ 0.05) as compared to other groups. ConclusionResults revealed that ethanolic extract of Azadirachta indica leaves in G2 group (@ 500 mg/kg of body weight) normalized lipid profile in dyslipidemic rabbits after 60 days of extract administration which significantly lowered TC, TG, LDL levels (P ≤ 0.05) and improved HDL level.

Comparative Analysis of Stable Aqueous Dispersion of Silver Nanoparticle Synthesized from Mangifera Indica and Azadirachta Indica Leaf Extract

Mango and neem leaf extract were prepared by soaking the measured leaves into 100ml of distilled water under appropriate conditions. Consequently, biosynthesis of silver nanoparticles was carried out by distinctly adding the mango and neem leaf extract to prepared solution of silver nitrate (AgNO3) to produce aqueous dispersion of silver nanoparticles. The silver nanoparticles produced were investigated using FTIR, UV-Visible and AFM Spectroscopy at 15 minutes, 24 hours and 48 hours respectively. Reduction in OH absorption proves chemical interaction of mango extract with AgNO3. UV/Visible also revealed colour changes from faint yellow to brown to reddish brown within 15 minutes and 24 hours of incubation at room temperature. Atomic Force Microscopy revealed that the sizes of mango-based dispersion are smaller than that of neem-based dispersion.

Antibacterial and Antiproliferative Activities of Azadirachta indica Leaf Extract and Its Effect on Oil-in-Water Food Emulsion Stability.

The present study aims to identify and quantify the phenolic compounds of Azadirachta indica leaf extract using HPLC-MS and to evaluate the antioxidant, antibacterial (against different Gram-positive and negative bacteria) and in vitro anti-proliferative activities of this extract (against breast, human liver and cervix adenocarcinoma-derived cells). The application of this extract as a natural antioxidant for food preservation was also tested on oil-in-water food emulsions for the first time in the present work in order to determine the use of Azadirachta indica leaves as a natural additive to preserve the food against lipid oxidation and rancidity. The results obtained revealed that 50%-aqueous ethanol leaf extract showed the best extraction yield (25.14%), which was characterized by a high content in phenolic compounds and strong antioxidant activity. Moreover, this leaf extract inhibited the growth of the bacterial strains tested (Staphylococcus aureus, Escherichia coli, Salmonella paratyphi and Micrococcus luteus) and showed better anti-proliferative activity against breast and cervix adenocarcinoma-derived cells than human liver cancer cells after 48 h of treatment. Additionally, Azadirachta indica leaf extract showed almost similar effects as gallic acid solutions (0.25% and 0.5%) in preserving the oxidation of oil-in-water food emulsions and prevented the formation of secondary oxidation products (malondialdehyde) as well. The results obtained suggested that extracts of Azadirachta indica leaves are a potential source of antioxidant and antibacterial compounds and pointed to the potential of these natural extracts as therapeutic agents.

Prospects of Methylene Blue degradation using green synthesised ZnO nanostructures

Nanomaterials have been used a lot, especially in optical, sensing, biomedical and manufacturing sectors, due to their piezoelectric, pyroelectric, photocatalytic and semiconducting properties. Green synthesized ZnO Nanostructures are biocompatible, eco-friendly, cost-effective and energy-efficient. One of the biggest challenges is the overuse of harmful textile dyes and their discharge from industry into environmental water. Before discharging, the effluent should be treated to degrade the dye into a non-toxic state. Conventional treatment procedures are unable to efficiently remove these toxic pollutants. Therefore, the need for the development of sustainable removal methods is essential. The present study focuses on the study of synthesis of ZnO Nanostructures utilising plant extracts and their application in the breakdown of Methylene Blue. ZnO Nanostructures can be used in dye degradation due to their semiconductor and photocatalytic properties. In plant-based synthesis, the plant extracts serve as both bio-reducing and capping agents. We have studied ZnO Nanostructures synthesised from the different parts of the plants such as K. septemlobus barks, S.baicalensis roots, Canna indica L flower, Artocarpus gomezianus fruit extract and Azadirachta Indica (Neem) leaf extract on the basis of various parameters like surface morphology and synthesising technique. Green synthesised ZnO Nanostructures are an economical and environmentally friendly approach, to the renewal of our environment.

Synthesis of silver-decorated nanocomposite based on reduced graphene oxide and its electrochemical performance

The formation of silver-decorated nanocomposite based on reduced graphene oxide has been carried out in a single step by using leaf extract of Azadirachta indica as a reducing agent. Various analytical techniques have been employed for exploring the properties of the synthesized Ag/rGO nanocomposite. XRD studies confirmed the cubic structure with a crystallinity of 70.3%. The FESEM and EDX analysis revealed that AgNPs having particle sizes in the range of 68–95 nm are symmetrically decorated on the rGO surface. The UV-Vis spectroscopy also confirmed the successful formation of AgNPs and reduction of GO into rGO with a tunable energy bandgap of 2.27 eV in Ag/rGO nanocomposite. The presence of various phytochemicals in the leaf extract, responsible for the in-situ formation of AgNPs and reduction of GO into rGO, has been confirmed through FTIR analysis. Furthermore, the electrochemical performance investigations of synthesized rGO and Ag/rGO nanocomposite as an electrode showed a maximum specific capacitance of 206 Fg−1 and 349 Fg−1 at 1 Ag−1 current density, respectively with outstanding long-cycle stability of 88.0% after 4000 cycles. It is expected that the present investigations of synthesized Ag/rGO nanocomposite may help to understand its behavior as an electrode material for device applications.

Efficacy of Azadirachta indica leaf, stem and bark extracts on seedling related traits of Phaseolus vulgaris L.

Efficacy of Azadirachta indica leaf, stem and bark extracts on seedling related traits of Phaseolus vulgaris L.

ANTIDIABETIC AND HEPATOPROTECTIVE EFFECTS OF COMBINED AQUEOUS LEAF EXTRACT OF Azadirachta indica AND Mangifera indica IN ALLOXAN MONOHYDRATE-INDUCED DIABETIC WISTAR RATS

This study was aimed at assessing the antidiabetic and hepatoprotective effects of combined aqueous leaf extract of Azadirachta indica and Mangifera indica in alloxan monohydrate-induced diabetic Wistar rats. Fresh leaves extract of Azadirachta indica and Mangifera indica were prepared using distilled water in appropriate stock concentrations and used in a 14-day treatment. Acute toxicity studies of the combined extract conducted in rats showed no toxicity up to 5000mg/kg. Experimental animals were acclimatized for 14 days while diabetes was induced using alloxan monohydrate (150mg/kg) intraperitoneally. After 14 days of treatment of diabetic rats with 300mg/kg, 500mg/kg 700mg/kg respectively as well as with 150mg/kg metformin, there was increase in weight, the blood glucose levels decreased significantly (p<0.05) whenc compared to untreated diabetic rats (diabetic control). Also, there was a significant p<0.05) reduction in serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) levels of rats treated with different doses of the extract compared to the diabetic control. A dose dependent antidiabetic and hepatoprotecitve effect was observed and the combined extract had a close similar effect as the standard drug metformin. Therefore, combined aqueous leaf extract of Azadirachta indica and Mangifera indica exhibit antidiabetic and hepatoprotective and this is due to the presence of phytochemical constituents in the leaf part of the plants. KEY WORDS: Azadirachta indica, Mangifera indica combined aqueous leaf extract, diabetes, hepatotoxicity, Bioactive constituents

In Vitro Antibacterial Activity of Green Synthesized Silver Nanoparticles Using Azadirachta indica Aqueous Leaf Extract against MDR Pathogens.

Rice is the most important staple food crop feeding more than 50% of the world's population. Rice blast is the most devastating fungal disease, caused by Magnaporthe oryzae (M. oryzae) which is widespread in rice growing fields causing a significant reduction in the yield. The present study was initiated to evaluate the effect of green synthesized silver nanoparticles (AgNPs) on the biochemical constituents of rice plants infected with blast. AgNPs were synthesized by using Azadirachta indica leaf extract and their characterization was performed using UV-visible spectroscopy, particle size analyser (PSA), scanning electron microscope (SEM), and X-ray diffraction (XRD) which confirmed the presence of crystalline, spherical shaped silver nanoparticles with an average size of 58.9 nm. After 45 days of sowing, artificial inoculation of rice blast disease was performed. After the onset of disease symptoms, the plants were treated with AgNPs with different concentrations. Application of nanoparticles elevated the activity of antioxidative enzymes such as superoxide dismutase, catalase, peroxidase, glutathione reductase, and phenylalanine ammonia-lyase compared to control plants, and total phenol and reducing sugars were also elevated. The outcome of this study showed that an increase in all biochemical constituents was recorded for A. indica silver nanoparticles-treated plants. The highest values were recorded in 30 ppm and 50 ppm AgNPs-treated plants, which showed the highest resistance towards the pathogen. Green synthesized AgNPs can be used in future for disease control in susceptible varieties of rice. The synthesized AgNPs using A. indica leaf extract have shown promising antibacterial activity when tested against 14 multidrug-resistant (MDR) bacteria comprising Gram-negative bacteria Escherichia coli (n = 6) and Klebsiella pneumoniae (n = 7) with a good zone of inhibition diameter, tested with the disc diffusion method. Based on these findings, it appears that A. indica AgNPs have promise as an antibacterial agent effective against MDR pathogens.

Azadirachta indica leaf extract mediated silver nanoparticles impregnated nano composite film (AgNP/MCC/starch/whey protein) for food packaging applications

In order to be used in food packaging, the study aims to develop a composite film based on microcrystalline cellulose (MCC) and coated with silver nanoparticles (AgNPs). The MCC was derived from sugar cane bagasse. Protein, starch, and poly-ethylene glycol 1500 (PEG-1500) are employed to improve the tensile strength, flexibility, and durability of the packaging film. The AgNPs was synthesized by a green route employing Azadirachtaindica leaf extract as reducing agent. The determined average crystallite size of AgNPs was seen at 20 nm. The X-ray diffraction (XRD) studies of the final film prepared have an elevated peak with a crystallinity of 37.5%. The scanning electron microscopic images (SEM) of the AgNPs and the prepared samples, reveal their surface morphology. The Fourier transform infrared spectroscopic studies (FT-IR) disclose the functional group changes during the film preparation. The antibacterial activity of the amalgamated AgNPs against five bacterial pathogens studied was found to be highly active against tested food pathogens, except for Proteus vulgari. When coated over a vegetable, the produced nanocomposite film displayed an increased shelf life for the vegetable by limiting the decay impact caused by food pathogens. According to the findings, the AgNPs-impregnated MCC/Starch/Whey protein has the potential to be employed as an antimicrobial packaging material.

Integrated approach for managing Fusarium wilt of chickpea using Azadirachta indica leaf extract, Trichoderma harzianum and Bavistin

Integrated approach for managing Fusarium wilt of chickpea using Azadirachta indica leaf extract, Trichoderma harzianum and Bavistin

Biosynthesis, Characterization, Evaluation, and Shelf-Life Study of Silver Nanoparticles against Cotton Bollworm, Helicoverpa armigera (Hubner) (Noctuidae: Lepidoptera).

Nanoparticles provide a promising and alternative platform of eco-friendly technologies that encompasses better cost-resilient remedies against one of the most economically harnessing insect pests of cotton. The main goal of this research was to provide a better management strategy through biologically synthesizing (sunlight exposure method) green nanoparticles from leaf extracts of Azadirachta indica and Pongamia pinnata and proving their bioefficacy on H. armigera (2nd instar). Characterization of bio-synthesized silver nanoparticles was carried out using UV-Visible spectroscopy for confirming the formation of nanoparticles, a Particle Size Analyzer (PSA) for determining the size/distribution of particles, and a Scanning Electron Microscope (SEM) for analyzing the surface topology of nanoparticles. The results obtained from PSA analysis showed that A. indica and P. pinnata-based silver nanoparticles had an average diameter of 61.70 nm and 68.80, respectively. Topographical images obtained from SEM proved that most of the green synthesized silver nanoparticles were spherical in shape. A. indica-based silver nanoparticles were found to be comparatively more efficient and have higher insecticidal activity compared to P. pinnata-based nanoparticles. A. indica-based AgNPs recorded larval mortality of 60.00 to 93.33 percent at the concentrations of 500 to 2000 ppm, followed by P. pinnata-based nanoparticles, with 60.00 to 90.00 percent larval mortality. Shelf-life studies revealed that A. indica-based AgNPs had the maximum negative zeta potential of −58.96 mV and could be stored for three months without losing bioefficacy and up to six months with negligible reduction in bioefficacy. Symptoms caused by silver nanoparticles were leakage of body fluids, sluggishness, inactiveness, brittleness, etc.

In Vitro Aggregation Inhibition Activity of n-Hexane Leaf Extract of Azadirachta indica of Human Platelet

a peer-reviewed, open access online international journal which publishes original research papers. The journal welcomes submission from scholars and experts for possible publication from all over the world. The scope of the journal includes: Pharmaceutical research, chemistry and biochemistry of naturally occurring compounds, biological evaluation of crude extracts, ethnomedicine, traditional and complementary medicine, ethnopharmacology, biomedical research, Biotechnology, Evaluation of natural substances of land and sea and of plants, microbes and animals, pharmacognosy, bioavailability, clinical, pharmacological, toxicological studies and pharmacokinetics of phytochemicals, Isolation and characterization of compounds, structure elucidation, synthesis and experimental biosynthesis of natural Product as well as developments of methods in these areas are welcomed in the journal.

Synthesis of steroidal dihydropyrazole derivatives using green ZnO NPs and evaluation of their anticancer and antioxidant activity

Zinc oxide nanoparticles (ZnO NPs) were synthesized by a green method using Azadirachta indica leaf extract. The structure of the prepared ZnO (NPs) were characterized by FT-IR, XRD, SEM-EDX and TEM analyses. The biosynthesized ZnO (NPs) were then used as a catalyst for the synthesis of steroidal dihydropyrazole derivatives through a one-pot multicomponent reaction involving phenyl acetylene and hydrazine derivatives. The anticancer activity of newly synthesized compounds were evaluated against three cancer cell lines namely HeLa (human cervical carcinoma), Hep3B (human hepatocellular carcinoma) and MCF7 (human breast adenocarcinoma) by MTT assay. The tested compounds were found to be active against all cancer cell lines and less toxic towards normal peripheral blood mononuclear cells (PBMCs). Antioxidant activity have also been investigated via free radical scavenging ability using DPPH, FRAP and ABTS assay. The tested compounds were found to exhibit moderate to good antioxidant activity which increases with increase in the concentration of steroidal dihydropyrazoles. Among all the tested steroidal dihydropyrazoles, compound 17 is found to be most active.

Biocontrol of Fruit Rot of Litchi chinensis Using Zinc Oxide Nanoparticles Synthesized in Azadirachta indica.

Lychee (Litchi chinensis Sonn.) is a famous fruit species of tropical and subtropical regions of the world and many biotic and abiotic stresses affect its yield. In this study, lychee fruit rot has been observed and its incidence has been controlled by using zinc oxide nanoparticles (ZnO NPs). Diseased lychee fruits were collected and diagnosed to identify disease-causing pathogens. Morphological appearance, microscopic observation, and sequence analysis of the amplified ITS region identified this isolated pathogen as Aspergillus niger. To control this problem, ZnO NPs were prepared in the leaf extract of Azadirachta indica. Before their antifungal activity, ZnO NPs were characterized using sophisticated approaches. FTIR revealed the presence of reducing and stabilizing molecules on ZnO NPs including alcohol, carboxylic acid, alkyl halide, amine, and alkyl halide. Crystalline nature and average size (29.024 nm) of synthesized ZnO NPs were described by X-ray diffraction. EDX analysis depicted the mass percentage of zinc (30.15%) and oxygen (14.90%). SEM analysis displayed the irregular shape of nanoparticles and confirmed the nano-size of ZnO NPs. Maximum mycelial growth inhibition (70.5%) was observed at 1.0 mg/mL concentration of ZnO NPs in vitro. In in-vivo disease-control analysis, maximum control of lychee fruit rot disease was observed at the same concentration. These results reveal the potential use of these ZnO NPs on a larger scale to replace hazardous chemical fungicides.