In today’s conditions of reforming the law enforcement agencies of Ukraine, it becomes relevant to study the psychological aspects of the professional activities of the National Police officers in the context of the fulfillment of their professional duties. The task of improving the psychological support of patrol police units is of particular importance. The aim of the study is to develop and substantiate the professional psychological profile of a modern patrol officer. On the basis of an empirical study, in which 290 police officers of the National Police of Ukraine participated, the main components of the psychogram of a patrol officer, which form a generalized professional psychological profile of a modern police officer, were identified. Having applied theoretical and empirical methods (the analysis and generalization of scientific literature, observation, questioning, psychological diagnostics, expert interrogation, mathematical statistics, and experiment), the theoretical principles of studying the professional activity of patrol officers, the requirements for their knowledge, skills and individual psychological characteristics were outlined. A number of valid multifactorial psychodiagnostic techniques were applied, in particular, in order to study the level and structure of intelligence; to explore creativity; to investigate the features of nervous processes; to identify the character and temperamental features; to study the features of interpersonal interaction. The study determined a list of professionally important qualities that are required for mastering the profession of a patrol officer, as well as psychological contraindications to acquiring this profession.