Lateral Transfer Research Articles

Constraints on the expansion of paralogous protein families.

Duplication and divergence is a major mechanism by which new proteins and functions emerge in biology. Consequently, most organisms, in all domains of life, have genomes that encode large paralogous families of proteins. For recently duplicated pathways to acquire different, independent functions, the two paralogs must acquire mutations that effectively insulate them from one another. For instance, paralogous signaling proteins must acquire mutations that endow them with different interaction specificities such that they can participate in different signaling pathways without disruptive cross-talk. Although duplicated genes undoubtedly shape each other's evolution as they diverge and attain new functions, it is less clear how other paralogs impact or constrain gene duplication. Does the establishment of a new pathway by duplication and divergence require the system-wide optimization of all paralogs? The answer has profound implications for molecular evolution and our ability to engineer biological systems. Here, we discuss models, experiments, and approaches for tackling this question, and for understanding how new proteins and pathways are born.

Active surveillance of the spread of mcr-1-positive E coli

Active surveillance of the spread of mcr-1-positive E coli

Clinical relevance and plasmid dynamics of mcr-1-positive Escherichia coli in China: a multicentre case-control and molecular epidemiological study

Although the emergence of the plasmid-mediated colistin resistance gene, mcr-1, caused global concern, little is known about its clinical implications and transmission characteristics over time. We aimed to investigate the clinical relevance of infection with mcr-1-positive Escherichia coli isolates and to investigate long-term plasmid dynamics. We did a multicentre case-control study and molecular epidemiological survey of mcr-1-positive E coli infections. E coli isolates from four hospitals in China in 2008 to 2017 were collected and screened for mcr-1 by PCR and Sanger sequencing. Patients with mcr-1-positive E coli infections and matched controls with mcr-1-negative E coli infections were included in a 1:4 ratio, considering age, sex, living environment, comorbidities, antimicrobials used, and clinical sample type as potential risk factors in a regression model. 28-day mortality was also observed. The genomes of all mcr-1-positive E coli were sequenced to explore their genetic background and map IS Apl1 elements. Plasmids carrying mcr-1 were characterised by their resistance profile and incompatibility group. 29 100 isolates were collected across all hospitals and during the study period, 300 (1·03%) of which were mcr-1-positive E coli. The overall proportion of mcr-1-positive isolates significantly increased from 0·42% (1 of 240) in 2008 to 1·39% (66 of 4748; p<0·0001) in 2017. Factors related to health-care contact, including receiving cancer care, indwelling central venous catheter, and hospitalisation in the past 3 months, and some sample types (pus secretion and sputum) were significantly associated with mcr-1-positive E coli infection. 28-day mortality was low in both mcr-1-positive (11 [4·4%] of 248 patients) and mcr-1-negative (39 [3·8%] of 1030 patients) groups and did not significantly differ. Although the genetic background of mcr-1-positive E coli was diverse, most of the mcr-1-encoding plasmids occurred in three dominant Inc groups (IncX4, IncI2, and IncHI2). Only the large IncHI2 plasmids conferred multiple resistances and probably integrated with other resistance plasmids. In 205 (68%) of 300 mcr-1-positive E coli isolates, mcr-1 genes lost their capacity for mobilisation because of loss of flanking IS Apl1 elements. The prevalence of mcr-1-positive E coli infection among patients increased over the study period, although it remained low. Health-care contact was the most probable risk factor. Plasmids are likely to have played a critical role in mcr-1 transmission, rather than clone dissemination and lateral transfer of IS Apl1. Our findings underscore the importance of continued surveillance of E coli strains positive for mcr-1 and potentially other resistance-associated genes, particularly in hospital settings. National Natural Science Foundation of China.

An ergonomic assessment of using laterally-tilting operating room tables and friction reducing devices for patient lateral transfers

Patient lateral transfers between two adjacent surfaces pose high musculoskeletal disorder risks for nurses and patient handlers. The purpose of this research was to examine the ergonomic benefits of utilizing the laterally-tilting function of operating room (OR) tables during such transfers – along with different friction-reducing devices (FRD). This method allows the patient to slide down to the adjacent surface as one nurse guides the transfer and another controls the OR table angle with a remote control. Sixteen nursing students and sixteen college students were recruited to act as nurses and patients, respectively. Two OR table angles were examined: flat and tilted. Three FRD conditions were considered: a standard blanket sheet, a plastic bag, and a slide board. Electromyography (EMG) activities were measured bilaterally from the posterior deltoids, upper trapezii, latissimus dorsi, and lumbar erector spinae muscles. The Borg-CR10 scale was used for participants to rate their perceived physical exertions. The efficiency of each method was measured using a stopwatch. Results showed that the tilted table technique completely replaced the physical efforts that would have been exerted by the pushing-nurse, in that muscle activation did not increase in the pulling-nurse. On the contrary, EMG activities of the pulling-nurse for most of the muscles significantly decreased (p < 0.05). The subjective Borg-ratings also favored the tilted table with significantly lower ratings. However, the tilted table required on average 7.22 s more than the flat table to complete the transfer (p < 0.05). The slide board and plastic bag were associated with significantly lower Borg-ratings and EMG activities for most muscles than blanket sheet, but they both were not significantly different from each other. However, they each required approximately 5 s more than the blanket sheet method to complete the patient transfer (p < 0.05). By switching from flat + blanket sheet to tilted + slide board, EMG activities in all muscles decreased in the range of 18.4–72.3%, and Borg-ratings decreased from about 4 (somewhat difficult) to 1 (very light). The findings of this study propose simple, readily available ergonomic interventions for performing patient lateral transfers that can have significant implications for nurses’ wellbeing and efficiency.

Molecular Dynamics Study of Bubble Nucleation on a Substrate with Nonuniform Wettability.

In the present study, the molecular dynamics simulation method is adopted to study bubble nucleation on a platinum substrate with nonuniform wettability. The central region of the substrate has strong hydrophilicity and both sides have weak hydrophobicity. It is interesting that the bubble nucleation happens in the hydrophobic region when the substrate temperature is low, and the nucleation position moves to the hydrophilic region with the increase of the substrate temperature. The intrinsic regime for the change of nucleation position with the substrate temperature is fully illustrated based on the competition between the suffered potential restriction and the absorbed thermal energy of liquid atoms. When the liquid atoms on one region obtain enough thermal energy to break their potential barrier, they convert into a bubble nucleus. Both the potential barrier for liquid atoms clinging to the substrate surface and the solid-liquid heat transfer efficiency improve with the enhancement of substrate hydrophilicity. The potential barrier is decided only by the atomic distribution and interatomic interaction. However, the substrate temperature changes the absorbed thermal energy of the liquid atoms within a specific time, causing the movement of the nucleation position. Furthermore, a hydrophilic nanostructure is introduced to replace the central smooth hydrophilic region and promote lateral heat transfer to the liquid on the hydrophobic region, leading to the improvement of the bubble nucleation efficiency.

The archaeal-bacterial lipid divide, could a distinct lateral proton route hold the answer?

The archaea-bacteria lipid divide is one of the big evolutionary enigmas concerning these two domains of life. In short, bacterial membranes are made of fatty-acid esters whereas archaeal ones contain isoprenoid ethers, though at present we do not have a good understanding on why they evolved differently. The lateral proton transfer mode of energy transduction in membranes posits that protons utilize the solvation layer of the membrane interface as the main route between proton pumps and ATPases, avoiding dissipation of energy to the bulk phase. In this article I present the hypothesis on a proton-transport route through the ester groups of bacterial phospholipids as an explanation for the evolutionary divergence seen between bacteria and archaea.ReviewersThis article was reviewed by Uri Gophna (Editorial Board member) and Víctor Sojo.

Research on roll stability of articulated engineering vehicles based on dynamic lateral transfer load

A 16-degree-of-freedom non-linear roll dynamic model is developed for an articulated engineering vehicle considering non-linear road excitations and non-linear characteristics of vehicle structures. In addition, a variable step-size numerical method is proposed to solve the non-linear dynamic model. The proposed numerical method can improve the calculation accuracy and the computational stability. Through the proposed dynamic model, an equation is derived considering time-varying tire load characteristics to reflect the roll stability of an articulated engineering vehicle. Using the proposed roll stability equation, the driving stability can be effectively evaluated for an articulated engineering vehicle with different system parameters. The analysis results show that the roll stability decreases significantly with the increase in vehicle speed, centroid height of engineering vehicle, or lateral slope angle. The influence of vehicle speed and lateral slope angle on roll stability is greater than that of the centroid height of engineering vehicle. When steering on the road with a lateral slope angle, the roll angle and the lateral load transfer ratio curves fluctuate with time. As the lateral slope angle increases, the fluctuation is stronger. Overall, the proposed model can accurately evaluate the roll stability of a driving articulated engineering vehicle and accurately determine the unstable tilting of an articulated engineering vehicle.

Effect of bentonite particles’ presence on two-dimensional chromium transmission

The co-transport of pollutants with colloidal particles to lower depths of groundwater and porous environments has been demonstrated in many studies in recent three decades. Despite the numerous researches, all experimental and numerical studies of pollutant transfer in the presence of colloidal particles have been carried out in one dimension, which causes significant errors in this phenomenon. In this study, the two-dimensional transfer experiment of chromium in the presence of bentonite colloidal particles is done in saturated porous media. In order to conduct the experiment in two-dimensional conditions, the sampling was done in central and lateral of the last experiment column section. The results have been demonstrated that the transmission along the longitudinal direction is higher than lateral in the three tests of the transfer of chromium, bentonite, and chromium in the presence of bentonite colloidal particles at the beginning of the experiment, and due to completed mixing in the section, it reached to a constant value as lateral samples. While the presence of bentonite colloidal particles facilitates the transfer of chromium in both longitudinal and lateral directions, increasing the bentonite colloidal particle concentration causes more getting stuck of colloid particles between the sand grains and reduction of the chromium transfer in both longitudinal and lateral directions. So, it can be concluded that transfer in the lateral direction is lower in bentonite colloidal particles compared with chromium, and the reason is the bentonite colloidal particles getting stuck between sand grains, which is exacerbated by increasing the concentration of the bentonite. Also, due to the chromium co-transport with colloid particles in the fraction of chromium total transport, increasing the bentonite concentration causes decreasing the chromium lateral transfer.

Vermamoeba vermiformis CDC-19 draft genome sequence reveals considerable gene trafficking including with candidate phyla radiation and giant viruses

Vermamoeba vermiformis is a predominant free-living amoeba in human environments and amongst the most common amoebae that can cause severe infections in humans. It is a niche for numerous amoeba-resisting microorganisms such as bacteria and giant viruses. Differences in the susceptibility to these giant viruses have been observed. V. vermiformis and amoeba-resisting microorganisms share a sympatric lifestyle that can promote exchanges of genetic material. This work analyzed the first draft genome sequence of a V. vermiformis strain (CDC-19) through comparative genomic, transcriptomic and phylogenetic analyses. The genome of V. vermiformis is 59.5 megabase pairs in size, and 22,483 genes were predicted. A high proportion (10% (n = 2,295)) of putative genes encoded proteins showed the highest sequence homology with a bacterial sequence. The expression of these genes was demonstrated for some bacterial homologous genes. In addition, for 30 genes, we detected best BLAST hits with members of the Candidate Phyla Radiation. Moreover, 185 genes (0.8%) best matched with giant viruses, mostly those related to the subfamily Klosneuvirinae (101 genes), in particular Bodo saltans virus (69 genes). Lateral sequence transfers between V. vermiformis and amoeba-resisting microorganisms were strengthened by Sanger sequencing, transcriptomic and phylogenetic analyses. This work provides important insights and genetic data for further studies about this amoeba and its interactions with microorganisms.

A novel strategy for metal transfer controlling in underwater wet welding using ultrasonic-assisted method

In this work, a new lateral ultrasonic-assisted droplet transfer controlling method was innovatively developed in the underwater wet welding process. The ultrasonic acoustic streaming can overcome the resistance caused by arc bubble rising, promote the metal transfer and reduce the proportion of droplet repelled spatter from 9% to 5%. The proportion of unstable droplet transfer mode decreased from 64% to 41%, which obtain a welded joint that was smooth and free of defects. The proportion of pro-eutectoid ferrite was decreased while the weld metal presented a finer microstructure. As a feasible and high-efficiency method, it is expected to spread the application of underwater wet welding in ocean and coastal engineering.

Lateral Transfer Assist Robot (LTAR): Development of a proof-of-concept prototype.

Falls during transfer to and from a wheelchair are associated with numerous problems. Factors responsible for difficulty in transferring include horizontal/vertical gaps between surfaces; obstacles, such as armrests; and complicated brake/footrests configurations before transferring. Moreover, controlling a wheelchair sufficiently close to the transfer surface within the confined home space is difficult. We described the design of the novel Lateral Transfer Assist Robot (LTAR) for solving problems during transfer. Furthermore, the effectiveness and usability of the robot were preliminary examined in healthy adults. The transfer problems and basic designs were organized. The effectiveness of the prototype was measured by three-dimensional motion analysis and questionnaire. The prototype LTAR was developed. With just a push on a button, the footplate lowers to the floor and the seat and armrest lowers to the height of the seating surface to fill the gap between the surfaces. Using these features, users can transfer by simply shifting their buttocks sideways. Additionally, LTAR has omnidirectional wheels that help move it within a narrow space. The LTAR was confirmed to reduce the physical and subjective burden, except for maneuverability. The LTAR was found to be effective for home use and reducing burden of transfer.

Quantum yield estimation for an electron-multiplying charge-coupled device from photon counting test data

Electron-multiplying charge-coupled devices (EMCCDs) allow for subelectron effective read noise and thus for imaging at extremely low flux levels. In the ultraviolet, quantum yield creates an additional source of stochastic gain variation, which can be difficult to quantify using existing techniques. We propose a method for measuring the quantum yield gain of these devices, independent of existing methods, using images that are part of the existing test regimen for new EMCCDs. With this method, we were able to recover the quantum yield used to create simulated images within an accuracy of ∼5 % and the method provided consistent results with test images after only minor modifications. However, the measured quantum yield remains anomalously low, consistent with other measurements on Teledyne-e2v devices. We hypothesize that this discrepancy is due to lateral transfer of secondary electrons between pixels at the surface explained by the band structure and crystal geometry of typical silicon wafers used in array detector manufacture.

Synchronized job transfer and task-specific human capital

Recent evidence suggests that frequent and broad lateral transfers increase the possibility of promotion. A question is how firms save the costs of these frequent lateral transfers given the existence of job-specific skills. This paper proposes a new theory of the transition across jobs based on the assumption that each job consists of tasks and that each of these tasks requires specific skills. This shows that firms synchronize the promotions and lateral transfers of their employees to reduce the cost of transferring to the employee those task-specific skills required for a new position. We find that this unique prediction is broadly supported by a large personnel data set in Japan.

Highly Ordered and Dense Thermally Conductive Graphitic Films from a Graphene Oxide/Reduced Graphene Oxide Mixture

Summary We report a new approach to making highly dense, oriented, and crystalline graphite films from heat-treated and pressed graphene oxide (G-O). By introducing small-diameter reduced graphene oxide (rG-O) flakes into the graphene oxide starting material, we found that after heat treatment at 3,000°C, the sample density and atomic order substantially improved over a film composed, at the outset, only of pure G-O flakes. A subsequent mechanical press increased the density but reduced the atomic order. A second 3,000°C heat treatment restored the graphitic structure with graphitization metrics exceeding even those of the first heat treatment. The optimized graphitic film with an original concentration of 15 wt % reduced G-O in G-O gave well-oriented graphitic films with a density of 2.1 g cm−3, cross-plane thermal conductivity of 5.65 W m−1 K−1, and in-plane thermal conductivity of 2,025 ± 25 W m−1 K−1.

834 Early Ambulation Initiative Following LE Grafts in Comparison to Our Center’s Traditional Standard of Care: A Retrospective Data Review

Abstract Introduction Our ambulation guideline following LE grafting is loosely defined as bedrest vs. lateral transfers only until POD#5. Patient’s mobility status is increased POD#5 with WB per physician discretion. A proposal was created to initiate mobility earlier than POD#5 for patients with LE grafts. Two of our three surgeons were in agreement with the early ambulation protocol. The third surgeon wished to follow the traditional ambulation practice guideline. This request was respected and made known to all staff for carryover. This afforded us the opportunity of a comparison group with our center’s traditional standard of care functioning as the control group. Methods The proposed early ambulation protocol was influenced by evidence-based practice guidelines as well as surgeon input. Criteria was based upon the location of grafting, graft crossing a joint, size of wound being grafted (&amp;lt; or = 400 cm), and general medical status of the patient. Our early ambulation protocol was established as follows: POD#1 Lateral transfers with involved LE elevated. POD#2 Dependent LE at edge of bed, WBAT short distance ambulation to chair or bathroom with AD. POD#3–4 Increase ambulation as tolerated, assess need for continued splinting, appropriateness of progressive ambulation, AROM exercises. POD# 4–5 Progress ambulation with AD as needed, stairs as needed for discharge, home exercise program. Our inclusion criteria consisted of patients of any age, LE burns with STSG not involving joints/involving joints with appropriate immobilization. Exclusion criteria consisted of fractures of involved LE, patients who were non-ambulatory at baseline, wounds &amp;gt;400 cm2, STSG to plantar aspect of foot, medically unstable patients, and surgeon discretion. Results Data reviewed over an eight month period of time yielded 27 patients who met our established criteria; 26 had no graft loss on the first dressing change. The patient with graft loss was attributed to graft placement directly over bone of the distal phalanx. Comparatively, no loss was noted in the control group with 10/10 patients ambulating on POD#5. Of note, early ambulation was granted for several patients with larger surface areas with no graft loss demonstrated, but were not included in this study. Conclusions In conclusion, 96% of patients demonstrated no graft loss with first dressing change following our early ambulation guideline. All patients who followed the traditional ambulation guideline demonstrated no graft loss on the first dressing change. Based on our findings, early mobility is not detrimental to graft integrity following specific established guidelines. Applicability of Research to Practice Our data supports early mobility of LE grafting up to 400 cm2 with 96% success rate. This may be supportive of further research with early mobility involving a larger surface area of LE STSG.

583 Impacting the Length of Stay of Burn Patients in the Emergency Department

Abstract Introduction Burn patients represent a challenging patient population and require early interventions. Advance Burn Life Support classes have been developed to guide first responders with assessing and treating burn patients in the pre-hospital setting. In the emergency department (ED) patients may experience hypothermia, delayed resuscitation, inadequate pain control, and delayed wound cares. Methods ED length of stay (LOS) was retrospectively reviewed for burn patient who met trauma activation criteria from 2013–2018. Patients were categorized based on ED disposition to burn stepdown, burn unit intensive care (BICU), or directly to the burn operating room (OR). Patients who died in the ED or were discharged home were excluded. In 2019, guidelines for burn activations, responses, and consults were developed to mirror the activation criteria for a Level 1 trauma institution. ED nurses and physicians were educated on burn assessment, wound care, and the new triage guidelines with emphasis on the importance of early transfer out of the ED. Results Prior to the change in burn activation guidelines, rapid transfer of burn patients out of the ED had not been emphasized. Of the 144 patients examined, ED LOS was 5.4 hours for those going to burn stepdown, 4.3 hours for BICU, and 3.7 hours for those who went directly to the burn OR. Several barriers to early transfer out of the ED were identified including lack of bed availability, lack of cleaning staff on nights and weekends, difficult lateral transfers of non-burn patients out of the Burn Unit, and a lack of education on the importance of early interventions by nursing staff. Since the implementation of the guidelines, there has been an improvement in LOS for BICU patients, but the goal of less than one hour in the ED has not yet been achieved. Conclusions Unacceptable ED LOS for burn patients were identified, triage guidelines were developed, and education was provided to staff. Guidelines and education, in conjunction with emphasizing the importance of having available beds in the Burn Unit, have led to a decrease in ED length of stay. Ongoing education and process improvement are key for rapid transfer of burn patients out of the ED. Applicability of Research to Practice Burn Centers can improve patient accounts by identifying areas that put burn patients at risk. Implementation of protocols can lead to process improvement and lead to better patient outcomes.

Excessive Promoters as Silencers of Genes Horizontally Acquired by Escherichia coli.

Horizontally acquired genes are usually transcriptionally inactive, although most of them are associated with genomic loci enriched with promoter-like sequences forming “promoter islands.” We hypothesized that lateral DNA transfer induces local mutagenesis, accumulating AT base pairs and creating promoter-like sequences, whose occupancy with RNA polymerase and a specific silencer H-NS suppresses the transcription of foreign genes. Error-prone mutagenesis was implemented for the “promoter island” of a foreign gene appY and the promoter region of an inherent gene dps. Derivatives with changed transcriptional activity were selected using a reporter plasmid pET28_eGFP. Only one cycle of mutagenesis with negative selection suppressed the activity of the main dps promoter to the background level due to a single substitution in its -10 element, while positive selection gave a sequence with improved -35 element, thus testifying feasibility of the approach. The same suppression for appY was achieved by three cycles, while eightfold transcription activation required nine iterations of mutagenesis. In both cases, the number of potential start points decreased resulting in an ordinary regulatory region with only one dominant promoter in the case of positive selection. Efficiency of H-NS binding remained virtually unchanged in all mutant constructs. Based on these findings we conclude that excessive promoters can adversely affect transcription by providing a platform for interference between several RNA polymerase molecules, which can act as a silencer at promoter-dense regions.

Effects of Longitudinal Vortices on Turbulent Junction Flow Heat Transfer

Turbulent junction flow is a vortex system upstream of a blockage embedded in a turbulent boundary layer, which leads to augmented heat transfer near the blockage. Vortex legs wrap around the blockage and travel downstream as longitudinal vortices. In multistage axial turbines or multiple row tube heat exchangers, these longitudinal vortices can collide with junction flow vortices further downstream. Furthermore, these devices often augment mixing and increase heat transfer with high freestream turbulence. A comprehensive study on how longitudinal vortices affect junction flow heat transfer for different Reynolds numbers, freestream turbulence values, and longitudinal vortex configurations was performed. Longitudinal vortices were generated upstream of a symmetric airfoil using delta winglet vortex generators. Spatially resolved endwall heat transfer coefficients upstream and around the junction were measured using infrared thermography. Time-averaged flowfield measurements of the incoming longitudinal vortices were taken using stereo particle image velocimetry. Longitudinal vortices provided limited heat transfer augmentation around the junction and did not disrupt the time-averaged horseshoe vortex. These interactions were identical at high turbulence, but longitudinal vortices were less effective at augmenting heat transfer, likely due to an increase in vortex wandering. Vortex wandering also led to a broadening in lateral heat transfer profiles.

Use of Geospatial, Hydrologic, and Geochemical Modeling to Determine the Influence of Wetland-Derived Organic Matter in Coastal Waters in Response to Extreme Weather Events

Flooding from extreme weather events (EWE), such as hurricanes, exports large amounts of dissolved organic matter (DOM) to both estuaries and coastal waters globally. Hydrologic connectivity of wetlands to adjacent river channels during flood events is potentially a major control on the DOM exported to coastal waters after EWEs. In this study, a geographic information system based flood model was used to: 1) determine the volume of flooded wetlands in a river corridor following Hurricane Matthew in 2016; 2) compute the resulting volume fluxes of DOM to the Neuse River Estuary-Pamlico Sound (NRE-PS), in eastern North Carolina and 3) use the flood model to quantify the wetland contribution to DOM export. The flood model-derived contributions were validated with a Bayesian Monte Carlo mixing model combining measurements of DOM quality: specific UV Absorbance at 254 nm (SUVA254), spectral slope ratio (SR), and stable isotope ratios of dissolved organic carbon (13C-DOC). Results indicated that 1) hydrologic connectivity of the freshwater riparian wetlands caused the wetlands to become the primary source of organic matter (OM) that was exported into the NRE-PS after Matthew and 2) this source lingered in these coastal waters in the months after the storm. Thus, in consideration of the pulse-shunt concept, EWE such as Hurricane Matthew cause pulses of DOM from wetlands, which were the primary source of the OM shunted from the terrestrial environment to the estuary and sound. Wetlands constituted ca. 48% of the annual loading of DOC into the NRE and 16% of DOC loading into the PS. Results were consistent with prior studies in this system, and other coastal ecosystems, that attributed a high reactivity of DOM as the underlying reason for large CO2 releases following EWE. Adapting pulse-shunt concept to estuaries requires the addition of a “processing” step to account for the DOM to CO2 dynamics, thus a new pulse-shunt process is proposed to incorporate coastal waters. Our results suggest that with increasing frequency and intensity of EWE, strengthening of the lateral transfer of DOM from land to ocean will occur and has the potential to greatly impact coastal carbon cycling.

Fault-related overpressure in the Krishna-Godavari Basin, India

Drilling and reaching to deeper target zones through an overpressured overburden formation in a structurally complex geologic setting requires robust geologic and geomechanical analysis to mitigate risk and control operational costs. These types of geologic conditions are present in the Krishna-Godavari Basin, where a series of horst and grabens defined by deep-seated faults and persistent high sedimentation rates through geologic time, result in the development of challenging conditions for exploratory drilling. We have developed possible overpressure mechanisms across the central part of the Krishna-Godavari Basin and its interplay through fault-related lateral pressure transfer. The basin sits over a horst, which is one of the many northeast–southwest-trending en echelon horst and graben structures comprising sediments from the lower Cretaceous to Holocene. In the study area, Paleocene formations in the horst are overpressured (12–12.2 ppg). Three wells were drilled through this formation and reached the target without any drilling issues in the central and eastern part. However, the same formation in the western part of the horst (adjacent to the graben) has higher overpressure of approximately 14 ppg, which complicates the drilling operations because it requires an additional intermediate casing to reach the target reservoir safely. A detailed analysis of the overpressure mechanisms across the horst area to the adjacent deep graben revealed that the disequilibrium compaction signatures are related to the burial history and overburden thickness. The major difference between horst and graben area is the magnitude of overpressure, with an average of 16 ppg across the graben area. The larger overpressures experienced toward the western part of the horst indicate a secondary source of pressure from the adjacent deep graben. The fault stress analysis in this region presents a feasible lateral pressure transfer through critically stressed faults/fractures from the deep graben to the western part of the horst structure. The current model accounts the common pore pressure estimation method along with other critical geologic information to predict such overpressure related challenges in the upcoming future wells in a similar geologic setup to plan safe and cost-effective wells.