Background: Correct movement techniques are crucial for enhancing athletic performance and minimizing the risk of injury. This is particularly true for kickboxing, in which a variety of forms of locomotion are employed. Despite its importance, gait and movement analysis in this sport is under-researched. The primary objective of this study is to understand gait patterns and limb symmetry between the dominant and non-dominant legs among professional kickboxers and to explore their correlation with the level of technical and tactical preparation. Methods: A cross-sectional observational study was conducted involving 20 elite kickboxers. Data collection employed the 3D Force Treadmill for gait parameters, as well as simulated sparring sessions to evaluate technical–tactical indices. Various gait parameters were analyzed, including the center of pressure (COP), gait phase, spatial and time parameters, as well as reaction force. Results: No significant bilateral differences were found in the majority of gait variables. Exceptions were in the medio-lateral component of the COP and force, where more deviation was observed in the non-dominant foot. The only noted significant correlation was between lateral symmetry and attack activity. Conclusions: This study allows us to indicate that kickboxers’ training promotes symmetrical gait patterns. Targeted training interventions could further optimize these patterns. The significant relationship between lateral symmetry and attack activity suggests areas for future research and potential performance improvement. The results of this study contribute to athletes’ self-monitoring and coaching strategies.