Later Stages Of Growth Research Articles

Water use characteristics of an early and late maturing maize hybrid using stable isotopes

AbstractWater requirements of early‐maturing (EM) maize hybrids might be lower than those of late‐maturing (LM) maize hybrids. This study aimed to analyze the effects of different irrigation management on grain yield (GY), water uptake patterns, evaporation (E), and transpiration (T) of two maize hybrids differing in maturity via stable isotope method. The field experiment with 36 lysimeters (4‐m wide × 4‐m long) under rain‐shelters was conducted with an EM (DH518) and a mid‐maturing and LM (DH605) hybrid with three replicates from 2021 to 2022. Maize plants were grown under three irrigation amounts (W1, W2, and W3 were irrigated to maintain soil moisture at 45%, 60%, and 75% of field capacity, respectively) by flood irrigation (FI) and drip irrigation (DI) under rain‐out shelters. Results showed that GY and evapotranspiration (ET) of EM were 8% and 12% lower, while the crop water productivity (CWP) was 6% higher than that of LM. Water uptake of both hybrids occurred mainly within 0‐ to 60‐cm soil layer during the vegetative stage. Water uptake of both hybrids occurred mainly within 0‐ to 60‐cm soil layer during the vegetative stage. Summer maize under FI absorbed more water from the deep soil layer than DI in the late growth stage. Compared with LM, the water absorption depth of EM became shallower in the late growth stage. Water uptake of maize was mainly from the deeper soil under water stress conditions. E of total ET accounts for 43% under DI and 57% under FI; GY and CWP were 22% and 51% higher than those of FI. Together with choosing EM hybrids, optimization of water management with DIW3 could achieve higher GY and CWP. The optimization of irrigation method and hybrids could provide a reference for local farmers to improve GY and CWP.

Crop Coefficient of Sunflowers under Drip Irrigation with Plastic Film in Different Hydrological Years in the Hetao Irrigation District

An accurate assessment of crop water requirements during the crop growth period can help organize irrigation schedules and investigate the hydrological environments in irrigation districts, especially in shallow groundwater districts. In irrigation scheduling, crop coefficients, representing three development stages, play a critical role in modeling evapotranspiration. Therefore, in this study, the crop coefficient (Kc) of sunflowers under drip irrigation in different hydrological years in the Hetao irrigation district with shallow groundwater is determined. Based on the analysis of rainfall frequency, the experimental situations of 2012, 2013, and 2014 are adopted as high, normal, and low flow years. Using the water balance method, groundwater recharge and crop evapotranspiration in different hydrological years were investigated. The results showed that the groundwater recharge in a high-flow year was larger than that in normal and low-flow years with values of 67.47, 66.75, and 42.61 mm, respectively. Crop evapotranspiration is directly related to the irrigation amount at the same growth stage. The Kc of sunflowers in a high-flow year is higher by 0.1 and 0.15 than that in normal and low-flow years. The Kc of sunflowers under drip irrigation is lower than that under surface irrigation by 8.9% in the initial and late stages of sunflower growth, but higher than that by 10.6% in the mid-stage, which is due to differences in small water potential differences and less evapotranspiration in the initial and late stages under drip irrigation. The study is of great significance for formulating a reasonable schedule of drip irrigation with shallow groundwater and improving the field environment.

Utilizing a DenseSwin Transformer Model for the Classification of Maize Plant Pathology in Early and Late Growth Stages: A Case Study of Its Utilization Among Zambian Farmers

Utilizing a DenseSwin Transformer Model for the Classification of Maize Plant Pathology in Early and Late Growth Stages: A Case Study of Its Utilization Among Zambian Farmers

Source-sink coordinated peanut cultivar increases yield and kernel protein content through enhancing photosynthetic characteristics and regulating carbon and nitrogen metabolisms

The grain yield of crops is determined by the synergistic interaction between source activity and sink capacity. However, source-sink interactions are far from being fully understood of peanut. Therefore, a 2-year field study (2018–2019) was conducted to compare differences in photosynthetic characteristics, carbon and nitrogen metabolism, and yield and quality of different source–sink peanut varieties. Four representative source–sink types were examined: JH5 (source–sink coordination type), SH9 (sufficient source–large sink type), ZH24 (sufficient source–few sink type), and HY36 (large source–few sink type). The results showed that the photosynthetic potential of HY36 was higher than that of the other varieties after flowering because of a large source (leaves), whereas the chlorophyll content and net photosynthetic rate of HY36 were significantly lower than those of JH5 and ZH24. Proportions of dry matter transferred to pods were significantly different among four source–sink peanut varieties. From 50 days after flowering, the dry matter distribution ratio of pods exceeded that of stems and leaves in JH5, significantly earlier than other varieties, which prolong the duration of pod-filling period, followed by SH9 and ZH24. The activities of nitrate reductase, glutamine synthetase, glutamate dehydrogenase, and glutamate synthase in JH5 were the highest among the varieties, and thus, the highest protein content was also in JH5. The activities of sucrose synthase and sucrose phosphate synthase in ZH24 were significantly higher than those in HY36. The highest oil content was also in ZH24. Among pod sink characteristics and yield, SH9 had the longest flowering period and the highest gynophore formation rate but the lowest pod-bearing rate, and the effective proportion and pod fullness were also lower than those of other varieties. The highest pod rate was in ZH24. The effective proportion and pod fullness of JH5 were higher than those of the other varieties, and its yield was also the highest, followed by SH9 and ZH24, with the lowest yield in HY36. The obtained results indicate that the source–sink coordinated variety had high Pn and chlorophyll content in the late growth stage, a long functional period of leaves, and a high proportion of assimilates transported to pods, thus promoting effective proportions and pod fullness to improve peanut yield and protein content, suggesting that different cultivation and management measures should select for different peanut varieties to best coordinate the relationship between the source and sink.

The impact of humic acid compound fertilizer on sweet potato variety Jishu 25: A comprehensive study on dry matter, root yield, and starch properties

In recent years, although humic acid (HA) compound fertilizers have been applied in sweet potato production, limited research has specifically addressed their impacts on dry matter (DM) accumulation, starch yield and starch properties, particularly in varieties with high starch content. To assess these effects, five treatments were designed for the high-starch variety Jishu 25 as follows: no fertilizer application (CK), chemical fertilizer with 75–60–180 kg ha−1 N-P2O5-K2O (F1), 750 kg ha−1 HA compound fertilizer with 2.6 % HA and 10–8–24 N-P2O5-K2O (F2), chemical fertilizer with 112.5–67.5–157.5 kg ha−1 N P2O5 K2O (F3), 750 kg ha−1 HA compound fertilizer with 3 % HA, and 15–9–21 N-P2O5-K2O (F4). The results indicated that F2 and F4 reduced the DM distribution ratio in leaves and stems, concurrently increasing DM accumulation in tuberous roots compared to F1 and F3. These HA fertilizer treatments enhanced the activities of AGPase, UGPase and SSS in tuberous roots during the middle and late growth stages, resulting in elevated total starch content and amylopectin content at harvest. Both types of HA fertilizers decreased the D [4,3] of starch particles, but exhibited minimal effects on PV, HPV, CPV, To, Tc and ΔH. The highest tuberous root yield and starch yield were observed after applying F2 continuously for two years. In conclusion, for optimizing starch yield while preserving its quality, the application of 750 kg ha−1 HA compound fertilizer with 2.6 % HA and 10–8–24 N-P2O5-K2O proved to be most advantageous for sweet potato producers cultivating this high-starch variety in regions with a similar climate in northern China.

Assessing the Effects of Biostimulant on Tomato Growth, Yield and Quality in Open Field Condition

Aim: This study, conducted at "C" Block Farm, Bidhan Chandra Krishi Viswavidyalaya, Kalyani, Nadia, West Bengal, during the period of 2022–23, explores the impact of biostimulants on the growth, yield, and quality of tomatoes (Lycopersicon esculentum Mill.). The primary objectives include investigating the significant effects of biostimulants and determining the optimal stage for their application, given the current emphasis on sustainable farming practices.
 Study Design: The experimental design employed Randomized Block Design (RBD) with three replications and eight treatments. Results indicate that biostimulant application, particularly as a foliar spray, significantly enhances crop growth and stimulates reproductive growth in later stages compared to control plants. Notably, the application of Biostimulant (Kazuki Gold)topically at 500 ml/acre during pre-flowering stage and during fruit development stage (T5) significantly registered the highest yield of 751.89 q/ha.
 Place and Duration of Study: The farm, situated near the Tropic of Cancer, exhibits coordinates of approximately 22.89°N latitude and 88.45°E longitude, with an altitude of around 9.75 m above sea level.
 Results: The highest lycopene content of 5.08 mg/100gm, which was significantly superior over all the treatments was obtained with the application of Kazuki gold during the two stages of application (i.e. at pre- flowering stages and during fruit development stage). The study also observed substantial impacts on other quality parameters with the application of seaweed extracts, protein hydrolysates, and N-fixing growth promoters during various stages of plant growth.
 Conclusion: Based on the findings, it can be concluded that Applying KAZUKI GOLD/YOSHI GOLD through foliar spray at a rate of 500ml per acre during the pre-flowering and fruit development stages has demonstrated profitability and advantages, leading to increased yields of high-quality tomato fruits.

Applying Nitrogen Fertilizer at the Full Heading Stage Has the Potential to Decrease Brown Rice Cd Accumulation

Soil contamination by cadmium (Cd) has presented a major challenge in China. The objective of the field experiments in this study was to examine the influence of nitrogen fertilizer application at the full heading and milky stages on minimizing the absorption of Cd in rice. This was achieved by affecting the distribution of Cd in root plaques and subcellular compartments of the root and flag leaf. The hydroponic culture experiments aimed to examine the effect of nitrogen and Cd interaction or deficiency on Cd accumulation in rice during the late growth stage. The findings revealed that adequate nitrogen supply during the early growth stage, coupled with nitrogen application during the full heading and milky stages, led to a notable increase in Fe concentration in the root plaques during the milk and mature stages. Furthermore, it elevated the Cd proportion in the soluble fraction of the flag leaves at the milky stage. Conversely, nitrogen deficiency during the early growth stage resulted in a significant increase in Fe concentration in the root plaques, along with a decrease in Cd concentration. Additionally, the proportion of Cd in the flag leaf cell walls increased significantly, while the proportion in the soluble fraction decreased notably. Irrespective of nitrogen supply during the early growth stage, applying nitrogen at the full heading stage significantly reduced Cd transport from shoots to brown rice, leading to a considerable reduction in the Cd concentration in brown rice. Under hydroponic culture conditions, combined Cd exposure with nitrogen supply significantly increased the Cd concentration in brown rice. Nitrogen supply had no impact on the Cd concentration in brown rice in the absence of Cd. The study showed that applying nitrogen fertilizer at the full heading stage effectively decreased the brown rice Cd concentration. This was achieved by elevating the concentration of Fe in the root plaque, thereby influencing the adsorption of Cd by the roots. Additionally, nitrogen application at the full heading stage can influence the distribution of Cd in flag leaf cells during the filling stage. Ensuring ample nitrogen supply in the early stage of rice growth is crucial, and nitrogen application at the full heading stage can effectively reduce the Cd concentration in brown rice.

Lateral bone ridge expansion and internal tissue replacement for vertebral body growth in Pacific bluefin tuna Thunnus orientalis.

Vertebral growth is an essential developmental process to support the expansion of the vertebrate body. In teleosts, the lateral side of the vertebral bodies develops to form different structures among species in the late stages of vertebral growth, although lateral structures are not apparent in the early stages. Lateral structures are one of the structural features that determine the diversity of teleost vertebrae. However, explanations for the formation of lateral structures are conflicting because few reports have investigated the growth of teleost vertebral bodies. To clarify the growth process, we analyzed the morphological changes in the vertebral body of Pacific bluefin tuna Thunnus orientalis at different developmental stages using micro-computed tomography(CT) scans. The micro-CT scans showed that the vertebral centrum formed a plate-like ridge on the lateral side along the cranial-caudal direction and extended laterally with increasing thickness. Simultaneously, the proximal region of the lateral ridges became porous as the vertebrae grew to form bone marrow cavities. Furthermore, we used histological observations to describe the relationship between these morphological changes and osteoblast and osteoclast activities. Osteoblasts accumulated on the distal edges of the lateral ridges, whereas osteoclasts were distributed in the bone marrow cavities. These observations suggest that bone resorption occurs proximally to form bone marrow cavities in addition to bone synthesis at the edges of the lateral ridges. The bone marrow cavities were occupied by blood vessels, extracellular matrix, and adipocytes, and the internal tissue composition changed to increase the area of adipose tissue. Because the ratio of bone volume decreases in large vertebrae, bone formation and resorption are regulated to separate the external cortical and internal trabecular bones to support the vertebrae. This study is the first to report the formation of lateral structures and can be applied to similar lateral structures in the vertebrae of other teleost species.

Population Distribution of Rats (Rattus argentiventer) and the Damage Intensity on Rice and Other Crops in Pleret, Yogyakarta, Indonesia

Rice field rats is one of the main pests of rice in Indonesia and other Asian countries that can cause a crop failure. The availability food sources might affect the rat foraging in the field. The research aimed to determine the distribution of rat population and their nests in the field with different crop and to examine the level of crop damage caused by rats. The research was conducted from January to April 2023 in Pleret district, Yogyakarta, Indonesia. The 1000 m2 TBS (Trap Barrier System) was installed in the middle of the 225 ha observation area to trap rats. Crop damage inside TBS was observed using a diagonal sampling method. Crop damage outside TBS was observed using the line transect method in the 4 cardinal directions with a distance of 250m, 500m, and 750m from TBS. Rat nest’s locations were mapped using GPS. The results showed that the number of rats trapped was low in the early and late stage of rice growth and peaked when the rice aged 12 weeks after planting (WAP). Rice crop damage inside TBS occurred when rice entered 12 WAP. There was no damage on rice crops around the TBS. Rats also attacked corn and sugarcane which caused heavy damage to these crops. There were 157 active holes found in 225ha observation area and they mostly were found in rice field bunds, irrigation embankments, and vacant land. This study implied that understanding the distribution of rat’s population and their nest will support the rat control.

Identification and Functional Characterization of a Novel Sinapyl Alcohol Acyltransferase from Euphorbia lathyris L.

Methoxyeugenol is a phenylpropene compound derived from plants and has various bioactivities. The chemical synthesis of methoxyeugenol is accompanied by pollution issues, whereas extraction from plants is associated with problems such as low yield and high cost. The production of methoxyeugenol can be effectively addressed through an enzymatic approach. In this study, the acyltransferase genes of Euphorbia lathyris L. were screened by homologous alignment of the transcriptome data of E. lathyris in the late growth stage and the acyltransferase genes of the closely related plant species. The results showed that ElBAHD10 had the closest relationship with earlier reported ScCFAT and PhCFAT, which were found to catalyze the reaction of coniferyl alcohol to generate coniferyl acetate. The ElBAHD10 gene was successfully cloned from E. lathyris and subsequently expressed in Escherichia coli. The purified protein ElBAHD10 catalyzed the reaction of sinapyl alcohol with acetyl CoA and cinnamoyl CoA to form sinapyl acetate and sinapyl cinnamate, respectively. In contrast, the crude ElBAHD10 protein could catalyze sinapyl alcohol to directly generate methoxyeugenol. The recombinant E. coli strain expressing ElBAHD10 produced methoxyeugenol through whole-cell transformation. This study provides insights and lays the foundation for methoxyeugenol production through biosynthetic approaches.

Dynamic serum testosterone concentration may predict in vitro fertilization yield during ovarian stimulation by a gonadotrophin‐releasing hormone agonist long protocol in women with normal ovarian reserve

AbstractObjectiveTo determine whether dynamic changes in serum total testosterone (TT) levels during controlled ovarian stimulation (COS) by a GnRH‐a (gonadotrophin‐releasing hormone agonist) long protocol may predict pregnancy in women with normal ovarian reserve in in vitro fertilization.MethodsThe TT measurements were added to routine hormone tests during COS. The TT dynamic changes, clinical pregnancy rate, and quality of oocytes and embryos of 109 patients were analyzed.ResultsCompared with the non‐pregnancy group, in the pregnancy group the TT level on Gn initial day (TTinitial‐d) increased sharply when the dominant follicle reached a diameter 10–12 mm [TTfΦ (10–12)‐d] and on human chorionic gonadotrophin (HCG) day (TTHCG‐d), also the ratios of TTfΦ (10–12)‐d to TTinitial‐d (1.23 ± 0.37 vs. 1.10 ± 0.58, p = 0.040) and TTHCG‐d to TTinitial‐d (2.32 ± 1.26 vs. 2.00 ± 1.43, p = 0.019) increased notably. Of the 2 TT ratios, the first tertile limit was regarded as the threshold of high TT ratios (1.00, 1.45). High tertiles had higher pregnancy rates than low tertiles (82.86% vs. 42.11%, p = 0.006; 71.43% vs. 44.83%, p = 0.040). The 2 TT ratios were positively associated with the number of metaphase II oocytes and good‐quality embryos.ConclusionOf COS in a long GnRH‐a protocol with optimized outcome, serum TT kinetics appears to be characterized by sharp rises at the early and late stages of follicle growth. The ratios of TTΦ (10–12)‐d to TTinitial‐d and TTHCG‐d to TTinitial‐d may be predictors for pregnancy and qualitative outcomes of oocytes and embryos.

Seaweed farming environments do not always function as CO2 sink under synergistic influence of macroalgae and microorganisms

Seaweed farming contributes substantial amounts of organic carbon to the ocean, part of which can be locked for a long term in the ocean and perform the function of ocean carbon sequestration, and the other part can be converted into inorganic carbon through microbial mineralization and aerobic respiration, affecting the pCO2, pHT and dissolved oxygen of seawater. It is generally believed that seaweed farming will cause the seawater to become a sink of CO2 due to carbon fixation by macroalgal photosynthesis. However, little attention has been paid to the fact that seaweed farming environment may sometimes become a source rather than a sink of CO2. Here, through in-situ mesocosm cultivation experiments and eight field investigations covering different kelp growth stages in an intensive farming area in China, we found that compared with the surrounding seawater without kelps, the seawater at the fast-growth stage of kelp was a sink of CO2 (pCO2 decreased by 17−73 μatm), but became a source of CO2 at the aging stage of kelp (pCO2 increased by 20−37 μatm). Concurrently, seawater pHT experienced a transition from increase (by 0.02−0.08) to decline (by 0.03−0.04). In-situ mesocosm cultivation experiments showed that the positive environmental effects (i.e., pCO2 decrease and pHT increase) induced by kelps at the early growth stage could be offset within only 3 days at the late-growth and aging stages. The release of dissolved organic carbon by kelps at the late growth stage increased significantly, supporting the enhancement in microbial abundance and respiration, which was manifested by the remarkable decrease in seawater dissolved oxygen, ultimately leading to CO2 release exceeding photosynthetic CO2 absorption. This study suggests that mature farmed kelps should be harvested in time to best utilize their carbon sink function and environmental benefits, which has guiding significance for the rational management of seaweed farming.

High Diversity of Epicoccum Species Associated with Leaf Spot on Italian Ryegrass in Southwestern China: Six New Records and Three New Species.

Italian ryegrass is widely cultivated for the production of forage, hay, and silage because of its high nutritional value and good palatability. Leaf spots caused by fungi pose a serious threat to forage crops. In order to expand the knowledge of fungi causing leaf spots in ryegrass (Lolium multiflorum) in Sichuan, Yunnan, Chongqing, and Guizhou of southwestern China, a comprehensive survey was undertaken from 2015 to 2022. The survey discovered that Epicoccum leaf spot (ELS) was a common and widespread disease, more serious at the late stage of growth (after late May). Symptomatic leaf samples collected from the four different provinces were analyzed, and a total of 202 Epicoccum isolates were obtained. Based on both multilocus phylogeny (ITS, LSU, TUB2, and RPB2) and morphology, 10 Epicoccum species were finally identified, including three novel species (E. endololii sp. nov., E. lolii sp. nov., and E. loliicola sp. nov.), six new host records (E. draconis, E. endophyticum, E. oryzae, E. plurivorum, E. thailandicum, and E. tobaicum), and an unknown species (Epicoccum sp.1). Pathogenicity tests showed that E. endophyticum, E. endololii, and Epicoccum sp.1 were nonpathogenic to Italian ryegrass, which were confirmed as endophytes in this study; the other six species could infect Italian ryegrass and cause leaf lesions to different degrees, of which E. draconis was more aggressive (P ≤ 0.05). Coupled with the isolation rates and geographical distributions of these species, it was found that E. plurivorum was the predominant pathogen in Yunnan while E. oryzae and E. tobaicum were the predominant pathogens in the other three provinces. This work provides an initial understanding of the taxonomy, virulence, and distribution of Epicoccum species associated with ELS in southwestern China and lays a solid foundation for the diagnosis in the field and scientific control of ELS on Italian ryegrass.

Optimized Farmland Mulching Improves Rainfed Maize Productivity by Regulating Soil Temperature and Phenology on the Loess Plateau in China

Owing to global warming, continuously increasing the grain yield of rainfed maize is challenging on the Loess Plateau in China. Plastic film mulching has been extensively utilized in dryland agriculture on the Loess Plateau. However, higher topsoil temperatures under film mulch caused rainfed-maize premature senescence and yield loss. Here, we aimed to explore the influence of topsoil temperature driven by novel double mulching patterns on rainfed maize productivity based on the excellent moisture conservation function of plastic film. A maize field experiment was conducted in two different areas, namely Changwu, a typical semi-arid area, and Yangling, a dry semi-humid area. The experiment followed a randomized block design with three replications. Five flat-planting practices were examined in 2021 and 2022: (1) bare land (CK), (2) transparent film mulching (PFM), (3) black film mulching (BFM), (4) double mulching of PFM with a black polyethylene net (PFM + BN), and (5) double mulching of PFM with whole maize stalks (PFM + ST). Soil hydrothermal conditions, maize growth dynamics, grain yield, water use efficiency (WUE), and economic returns were quantified under different mulching practices. Under double mulching treatments, topsoil temperatures were lower than PFM by 1.7–2.0 °C at the two sites (p < 0.05), whereas BFM was slightly lower than that of PFM by 0.6–0.7 °C at Yangling (p > 0.05). The average growth period for maize under double mulching was longer than that under PFM by 8–11 days at the two sites. Double mulching treatments significantly improved the leaf area index (LAI), chlorophyll relative content (SPAD), and aboveground biomass compared to CK and PFM during the late growth stage. Compared with PFM, average grain yield increased by 14.93%, 18.46%, and 16.45% in Changwu (p < 0.05) under BFM, PFM + BN, and PFM + ST, respectively, and by 2.71%, 24.55%, and 20.38% in Yangling. The corresponding WUEs also increased. Additionally, net income under BFM was higher than that under other treatments, and there were no significant (p > 0.05) differences between PFM + ST and BFM in Changwu. However, PFM + ST in net income averaged 10.72–52.22% higher than other treatments, and its output value was 19.51% higher in Yangling. In summary, smallholder farmers can adopt PFM + ST to improve rainfed-maize productivity in the Loess Plateau in China.

294 KISS1 Knockout Boars Have Decreased Concentrations of Gonadotropins Leading to Smaller Testes and Reduced Skatole in Backfat

Abstract In swine production, boars are castrated to prevent boar taint from testicular androgens and skatole accumulation in fat. Kisspeptin (KISS1) regulates secretion of gonadotropins and gonadal steroids. We used boars from our line of KISS1-/-pigs (10.3389/fgene.2022.1078991) to understand how loss of KISS1 function affects compounds causing boar taint. Boars were fed ad libitum during development. Body weights and testicular volume (TV) were collected at weaning, 40, 70, 100, 130, 160, and 190 d of age using TV = 4/3*π *a*b2*2 and subtracting scrotal skin thickness measured by a Harpenden skinfold caliper. Average daily gain (ADG) was calculated by subtracting the beginning from ending body weight at each age range and dividing by the number of days. At each age, a blood serum sample was collected by jugular venipuncture to quantify changes in hormones during development. Serum concentrations of luteinizing hormone (LH), follicle-stimulating hormone (FSH) and testosterone were quantified with radioimmunoassay and analyzed as repeated measures with genotype (KISS1+/+; KISS1+/-; KISS1-/-; n = 8 – 20 animals per genotype) and age as the fixed effects. Concentrations of androstenone and skatole in backfat (quantified using ELISA and HPLC, respectively) were log transformed and analyzed with ANOVA. Body weight did not differ among KISS1 genotypes but increased normally with age (P < 0.0001). During development, KISS1+/+ and KISS1+/- boars grew at similar rates. Although the ADG of KISS1-/-boars was similar to other genotypes up to 160 d of age, ADG was reduced at 190 d of age (age x genotype; P < 0.05). Mean concentrations of LH and FSH in KISS1+/+ and KISS1+/- boars were similar throughout development (LH, 1.16 ±0.11 and 1.34 ± 0.17 ng/mL; FSH, 0.88 ± 0.06 and 0.89 ± 0.05 ng/mL, respectively), and were greater than KISS1-/- boars (LH, 0.38 ± 0.23 ng/mL; FSH, 0.56 ± 0.07 ng/mL; P < 0.05). TV of KISS1+/+ and KISS1+/- boars did not differ during development and was greater than KISS1-/- boars (P < 0.05), for which testes could not be palpated after 100 d of age. Testosterone concentrations in KISS1+/+ and KISS1+/- increased 40-fold during development but were below detectable limits in KISS1-/- boars (age x genotype; P = 0.06). Androstenone and skatole in KISS1-/- boars (0.30 ± 1.24 µg/g and 12.6 ± 1.6 ng/g, respectively) at 190 d of age was less (P < 0.0001) than in KISS1+/+ and KISS1+/- boars (androstenone, 4.33 ± 1.16 µg/g; skatole, 69.2 ± 1.2 ng/g), which did not differ from one another. Thus, a single functional allele of KISS1 is sufficient to confer normal secretion of reproductive hormones, testicular growth, and skatole in boars. Moreover, knocking out the KISS1 gene will prevent boar taint, but might slightly reduce growth in later stages of development.

Design and test of powerful air-assisted sprayer for high stalk crops.

The canopies of high stalk crops, such as maize, intersect the rows at the later stages of growth, making conventional sprayers unable to enter the field for spraying. Air-assisted sprayers are often used to improve the deposition of droplets inside the canopy. In this study, the sprayer structure, the air-assisted system, and the spraying system were designed. The air-assisted conveyor system characteristics were numerically analyzed, and the wind-field distribution was tested. The wind-field distribution results showed that the near-ground wind speed exceeded 5 m s-1 in the sampling interval from 10 to 35 metres. The wind field covered a concentrated spatial area with a downward pressure trend, resulting in better drift resistance and penetration. Field tests for droplet distribution were conducted at three maize heights to verify the powerful air-assisted sprayer's technical performance and working quality. The test results showed that the droplet deposition and coverage decreased gradually along the range direction, and the top layer had the highest deposition and coverage across the canopy. The upper canopy of 0 to 12 metres range demonstrated a greater extent of coverage and deposition. The peak deposition area expanded from 9 to 33 metres in the lower canopy, with an average value of 3.77 μg cm-2. The droplet coverage within the 30 to 60 metres range only amounted to 15% to 18% of the total coverage.

High trophic level organisms and the complexity of soil micro-food webs at aggregate scale regulate carbon accumulation in cropland soils

Enhancing soil organic carbon (SOC) levels via improved fertilization strategies can promote soil health and support sustainable crop production. While bacteria and fungi play a key role in SOC accumulation, the effects of soil micro-food webs that include organisms from higher trophic levels remain uncertain. We sought to narrow this knowledge gap by clarifying the associations between SOC accumulation and soil micro-food webs at the aggregate scale under long-term (18 years) fertilization treatments, which included a no-fertilizer control (Ctrl), synthetic fertilizers (NPK), and partial synthetic nitrogen replacement with pig slurry (OMNPK) or crop straw (RSDNPK). The concentration of SOC was significantly increased by fertilization following the order RSDNPK (13.1 g kg−1) > OMNPK (11.4 g kg−1) > NPK (9.1 g kg−1) > Ctrl (6.4 g kg−1). Nematodes and protists accounted for approximately 20% of all nodes in the multitrophic networks. Fertilization increased network complexity at the aggregate scale by 1–4 times across different sampling dates (P < 0.05). In all aggregate classes, the functionally important (FI) nematodes, protists, and bacterial taxa involved in soil carbon cycle had higher relative abundances under the fertilization treatments than under the Ctrl (P < 0.05). Structural equation modeling further indicated that besides soil microbes, network complexity, FI nematodes and protists were important to SOC accumulation. Compared with NPK, the key moments during which these soil biological attributes strongly regulated SOC under OMNPK and RSDNPK were delayed to late growth stages of maize. In conclusion, the increased network complexity and relative abundance of functionally important organisms from higher trophic levels of the soil micro-food webs at certain periods are vital contributors to effective SOC accumulation under fertilization regimes that include organic amendments.

Productivity and Econometric Analysis of Leguminous Monkey-nut (Arachis hypogaea L.) as Influenced by Growth Retardant

Background: Groundnut (Arachis hypogaea L.) holds significant importance as a staple food legume and a key economic crop, serving as a valuable source of edible oil and protein in India. However, its full yield potential is often hampered by excessive vegetative growth, which leads to suboptimal yields. With this background, a field experiment was conducted at the Perunthalaivar Kamaraj Krishi Vigyan Kendra in Puducherry during 2020 and 2021. The objective was to investigate the influence of different concentrations and timing of paclobutrazol (PBZ) application on the growth and yield of groundnut. Methods: The experiment was laid out in a split-plot design with three replications, six main plot treatments (Including PBZ concentrations of 25, 50, 100, 150, 200 ppm and a control) and three sub-plot treatments (Involving single sprays at 30 days after emergence (DAE), single sprays at 50 DAE and double sprays at 30 and 50 DAE). Result: The results revealed that the application of paclobutrazol at different concentrations had a positive effect on reducing plant height during the later stages of growth, particularly when a double spray was applied at 30 and 50 DAE. Notably, the application of paclobutrazol at a concentration of 200 ppm in groundnut cultivation proved to be economically viable, resulting 29% increase in pod yield compared to the control group. Specifically, PBZ at 200 ppm, with a double spray, significantly boosted the total pod yield to 2724 kg ha-1. Furthermore, the correlation and regression analyses indicated a positive relationship between growth and yield.

192 Effect of Dietary Anhydrous Calcium Chloride in Early and Late Finishing on Pig Performance and Body Composition

Abstract Understanding how to control pig growth is critical during market disruptions, including foreign animal disease and harvest facility shutdowns. In times of severe market disruptions, feeding acidogenic salts such as anhydrous calcium chloride to suppress appetite is an effective way to manage pig growth. Therefore, the aim of this study was to understand the impact of feeding calcium chloride during early and late growth stages on grow-finish growth and body composition of pigs. Barrows and gilts (n = 240; 52 ± 6.9 kg BW) were allotted by sex across 60 pens and to one of three treatments fed in 3 diet phases (Phase 1, d 1-28; Phase 2 d 28-56; Phase 3 d 57-87). Treatment included: 1) Control corn-soy diets in phases 1-3, 2) 3% CaCl2 intervention in phase 1, control diet fed in phases 2 and 3 (IP1), and 3) Control diet fed in phases 1 and 2, and 3% CaCl2 intervention in phase 3 (IP3). All diets were formulated to meet or exceed amino acid and energy requirements and pigs were fed ad libitum. Pig BW were recorded at the start and end of each phase, and pen feed disappearance was recorded to calculate pen ADG, ADFI, and G: F. Loin eye area and fat depth were measured using real-time ultrasound at the end of phases 1 and 3. Data were analyzed with pen as the experimental unit and treatment, gender, and their interaction as fixed effects. No noteworthy gender by diet interactions were demonstrated and only the diet treatment, overall performance, and ultrasound trait means are reported (Table 1). Compared with the Control and IP3, IP1 reduced ADFI and ADG by 42% and 60%, respectively, in phase 1 (P&amp;lt; 0.05). Feed efficiency, loin eye area, and fat depth were also reduced in the IP1 compared with the Control and IP3 pigs in phase 1 (P&amp;lt; 0.05). In phase 2, the IP1 pigs did not have a significant difference in feed intake and their ADG increased by 22% compared with the Control and IP3 pigs (P&amp;lt; 0.05) and therefore were more feed efficient. Implementation of CaCl2 in phase 3, the IP3 pigs reduced ADFI, ADG, and G: F compared with the control and IP1. Further, phase 3 loin eye area and fat depth were 13% and 17% less, respectively, in the IP3 pigs compared with the control. Overall, the IP3 treatment had the most severe reductions in ADG, ADFI, and feed efficiency (P&amp;lt; 0.05). Early intervention (IP1) also attenuated overall G: F, ADFI, and ADG compared with the Control (P&amp;lt; 0.05). These results demonstrate the efficacy of calcium chloride in attenuating pig feed intake and growth performance during early and late finishing.

Optothermal crystallization of hard spheres in an effective bidimensional geometry.

Using colloids effectively confined in two dimensions by a cell with a thickness comparable to the particle size, we investigate the nucleation and growth of crystallites induced by locally heating the solvent with a near-infrared laser beam. The particles, which are "thermophilic," move towards the laser spot solely because of thermophoresis with no convection effects, forming dense clusters whose structure is monitored using two order parameters that gauge the local density and the orientational ordering. We find that ordering takes place when the cluster reaches an average surface density that is still below the upper equilibrium limit for the fluid phase of hard disks, meaning that we do not detect any signof a proper "two-stage" nucleation from a glass or a polymorphic crystal structure. The crystal obtained at late growth stage displays a remarkable uniformity with a negligible amount of defects, arguably because the incoming particles diffuse, bounce, and displace other particles before settling at the crystal interface. This "fluidization" of the outer crystal edge may resemble the surface enhanced mobility giving rise to ultra-stable glasses by physical vapor deposition.