In this paper, a heat-flow coupling model of laser welding at preheating temperature was established by the FLUENT 19.0 software. The fluctuation of the keyhole wall and melt flow behavior in the molten pool under different preheating temperatures were analyzed, and the correlation between keyhole wall fluctuation and molten pool flow with spatters and bubbles was obtained. The results indicate that when the outer wall in the middle of the rear keyhole wall is convex, the inner wall is concave, which causes spatter or the bottom of the keyhole to collapse. When the metal layer in the middle of the rear keyhole wall turns into obliquely upward flow, welding spatter is generated. In contrast, the metal layer in the middle of the rear keyhole wall changes to flow into the keyhole, and the bottom of the keyhole collapses. When the preheating temperature is 300 K (ambient temperature), 400 K, and 500 K, the inner wall in the middle of the rear keyhole wall is concave. With the increase in the preheating temperature, the area of the concave gradually increases, and the size of the liquid column behind the keyhole opening gradually decreases. When the preheating temperature is 300 K, there are more spatters above the molten pool. In comparison, when the preheating temperature is 400 K or 500 K, there are less spatters, and the bottom of the keyhole collapses.