Western Larch Research Articles

Breeding without Breeding: Is a Complete Pedigree Necessary for Efficient Breeding?

Complete pedigree information is a prerequisite for modern breeding and the ranking of parents and offspring for selection and deployment decisions. DNA fingerprinting and pedigree reconstruction can substitute for artificial matings, by allowing parentage delineation of naturally produced offspring. Here, we report on the efficacy of a breeding concept called “Breeding without Breeding” (BwB) that circumvents artificial matings, focusing instead on a subset of randomly sampled, maternally known but paternally unknown offspring to delineate their paternal parentage. We then generate the information needed to rank those offspring and their paternal parents, using a combination of complete (full-sib: FS) and incomplete (half-sib: HS) analyses of the constructed pedigrees. Using a random sample of wind-pollinated offspring from 15 females (seed donors), growing in a 41-parent western larch population, BwB is evaluated and compared to two commonly used testing methods that rely on either incomplete (maternal half-sib, open-pollinated: OP) or complete (FS) pedigree designs. BwB produced results superior to those from the incomplete design and virtually identical to those from the complete pedigree methods. The combined use of complete and incomplete pedigree information permitted evaluating all parents, both maternal and paternal, as well as all offspring, a result that could not have been accomplished with either the OP or FS methods alone. We also discuss the optimum experimental setting, in terms of the proportion of fingerprinted offspring, the size of the assembled maternal and paternal half-sib families, the role of external gene flow, and selfing, as well as the number of parents that could be realistically tested with BwB.

Evaluating tree competition indices as predictors of basal area increment in western Montana forests

Fire hazard reduction treatments are commonly applied to mixed-species coniferous forests in western Montana, USA, to modify fuels structures and alter the competitive environments of individual trees. An improved understanding of how competition can be measured and how it conditions individual tree growth is needed for projecting the development of these forests, with and without treatment. Numerous studies have evaluated how competition affects tree growth and many indices have been developed to quantify the competition an individual tree experiences. These studies suggest that no single competition index or a single class of indices is universally superior; indices perform differently according to forest type and forest conditions. We chose several widely used distance-independent and distance-dependent competition indices, and also derived anisotropic distance-dependent indices from estimates of light interception by tree crowns. We evaluated the effectiveness of these competition measures for predicting basal area increment (BAI) of Pinus ponderosa, Pseudotsuga menziesii, and Larix occidentalis in western Montana. The best distance-dependent competition indices explained a larger proportion of growth variation than the best distance-independent indices (64% vs. 56%). This result indicates that competition is an important growth determinant in these forests and that competition varies locally, with variable tree densities and relatively complex stand structures creating heterogeneous neighborhood conditions. Competition indices derived from light interception models were only weakly correlated with other indices and performed poorly in terms of predicting tree growth. This result accords with previous observations that competition for light is not the primarily growth limitation for trees in the semi-arid conditions of western Montana. More sophisticated light availability models could be used to better assess variability in light interception and its marginal contribution to predictive accuracy of radial tree growth. Diameter and distance-dependent BAI models were developed for growth prediction at the species level and for all species combined.

Field Tests of Naturally Durable Species

Six wood species generally accepted to represent a range of natural durability were exposed in American Wood Protection Association (AWPA) E7-09 (ground-contact) and AWPA E25-08 (aboveground) decay tests at field sites located near Maple Ridge, British Columbia, and Petawawa, Ontario, in Canada and Gainesville, Florida, and Hilo, Hawaii, in the United States. Variables examined included comparisons of sapwood to heartwood, old growth to second growth, and effect of a protective coating. The tests began between October 2004 and February 2005. Results are reported after 5 years of exposure. Ground-contact decay rates were fastest at sites in Florida and Hawaii. Yellow cedar (Callitropsis nootkatensis (D. Don) Örsted), western red cedar (Thuja plicata Donn ex D. Don), and eastern white cedar (Thuja occidentalis L.) had the highest condition ratings (least decay) for this measure after 5 years of exposure, followed by western larch (Larix occidentalis Nutt.), Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco), and then tamarack (Larix laricina (Du Roi) K. Koch). The aboveground decay rate was highest in Hawaii. For this measure, yellow cedar and western red cedar again had the highest average decay ratings (least decay) after 5 years of field exposure, followed by Douglas-fir, western larch, and tamarack. Eastern white cedar did not fit neatly into this pattern. It was durable at three of the four sites but failed rapidly in Hawaii. Sapwood appeared to have a larger impact on aboveground decay than on ground-contact decay. No substantial difference was found between old-growth and second-growth decay rates.

Estimation of canopy average mesophyll conductance using δ13C of phloem contents

Conductance to CO(2) inside leaves, known as mesophyll conductance (g(m)), imposes large limitations on photosynthesis. Because g(m) is difficult to quantify, it is often neglected in calculations of (13)C photosynthetic discrimination. The 'soluble sugar method' estimates g(m) via differences between observed photosynthetic discrimination, calculated from the δ(13)C of soluble sugars, and discrimination when g(m) is infinite. We expand upon this approach and calculate a photosynthesis-weighted average for canopy mesophyll conductance ((c) g(m)) using δ(13)C of stem phloem contents. We measured gas exchange at three canopy positions and collected stem phloem contents in mature trees of three conifer species (Pseudotsuga menziesii, Thuja plicata and Larix occidentalis). We generated species-specific and seasonally variable estimates of (c)g(m) . We found that (c)g(m) was significantly different among species (0.41, 0.22 and 0.09 mol m(-2) s(-1) for Larix, Pseudotsuga and Thuja, respectively), but was similar throughout the season. Ignoring respiratory and photorespiratory fractionations ((c)Δ(ef)) resulted in ≈30% underestimation of (c)g(m) in Larix and Pseudotsuga, but was innocuous in Thuja. Substantial errors (~1-4‰) in photosynthetic discrimination calculations were introduced by neglecting (c)g(m) and (c)Δ(ef) . Our method is easy to apply and cost-effective, captures species variation and would have captured seasonal variation had it existed. The method provides an average canopy value, which makes it suitable for parameterization of canopy-scale models of photosynthesis, even in tall trees.

Sulfometuron methyl influences seedling growth and leaf function of three conifer species

Seedling growth and gas exchange responses were measured for two potted seedling trials testing herbicide phytotoxicity to three important tree species of the Inland Northwest, USA. Media-filled pots were treated with sulfometuron methyl (Oust®) in varying concentrations and planted with seedlings of Larix occidentalis Nutt., Pseudotsuga menziesii var. glauca (Beissn.) Franco, and Pinus monticola Dougl. ex D. Don. Seedlings were grown in two trials to determine the effects of two important residue breakdown factors, substrate moisture and pH, relative to that of herbicide application rate on seedling health. Changes in seedling height, root-collar diameter, and root volume were morphologic variables of interest, and physiological variables measured were net photosynthetic assimilation, stomatal conductance, and transpiration rate. While three levels of media moisture and four levels of media pH had no effect on seedling performance, most growth and leaf function variables were hindered across application rate treatments of all three species. Label-recommended dosages resulted in growth suppression levels potentially detrimental to seedling establishment for all three species. This was most pronounced for Pinus monticola, in particular for root growth, where untreated control seedlings showed 109% more root volume growth than treated seedlings. We conclude that when possible, a species-specific application rate might be found that balances the benefits of vegetation control with the phytotoxicity to promote optimal growth gains.

Vertical and seasonal variation in the 13C of leaf-respired CO2 in a mixed conifer forest

The C-isotopic composition (δ¹³C) of leaf respiration (δ(LR)) has previously been shown to vary among functional groups, plant organs and times of day. We here investigated vertical and seasonal variation in δ(LR) through deep (~35 m) forest canopies. We measured δ(LR), δ¹³C of leaf bulk organic matter (δ(LB)), specific leaf area, net photosynthesis (A) and dark respiration in shade, middle and sun foliage in four conifer species from May to August. We used Keeling plots to estimate δ(LR); we developed a novel technique for ensuring that the respiratory substrate was not changing over the course of the measurement. Variables δ(LR) and δ(LB) displayed a vertical pattern in Abies grandis, Pseudotsuga menziesii and Thuja plicata, but were independent of canopy position in Larix occidentalis. Vertical gradients in δ(LB) (3.6‰) and δ(LR) (2.8‰) were similar. The respiratory enrichment (δ(LR)-δ(LB)) was smaller in expanding (3‰) than mature (4-8‰) foliage. There was a linear relationship between the respiratory enrichment and A. Our data support the hypothesis that δ(LR) values are related to patterns of C allocation among metabolic pathways. We demonstrated that considerable variation in δ(LR) occurs vertically through the canopy (3‰ gradient) and seasonally (3-7‰). Understanding sources of variation in respiratory signals is fundamental to comprehending C dynamics and for global model applications.

Congruence between parental reproductive investment and success determined by DNA-based pedigree reconstruction in conifer seed orchards

Seed orchards’ parental reproductive success is often assessed through surveys of seed- and pollen-cone production (reproductive investment). Although the reliability of this approach has been questioned, its speed and ease made it the method of choice for providing approximate estimates for the genetic quality and quantity of seed orchard crops. In this study, reproductive investment data from various female and male phenotypic assessment methods from three seed orchards (lodgepole pine ( Pinus contorta Dougl. ex. Loud. var. latifolia Engelm.), Douglas-fir ( Pseudotsuga menziesii (Mirb.) Franco), and western larch ( Larix occidentalis Nutt.)) were compared with reproductive success estimates based on DNA fingerprinting and parentage analyses. The use of reproductive investment as a proxy to reproductive success on estimating seed crop’s volume genetic worth and diversity (measured by effective number of parents) was evaluated. Regression analyses indicated that the phenotypic assessment methods reflect the true parental reproductive success and that estimates of seed- and pollen-cone volume production appear to be the most appropriate indicators of female and male reproductive success, respectively. Although the best combination of female–male survey methods produced inflated effective number of parents estimates, the genetic worth estimates were similar to those based on DNA, highlighting their robustness to fertility variation assessment.

Influences of climate, fire, grazing, and logging on woody species composition along an elevation gradient in the eastern Cascades, Washington

Across western North America, current ecosystem structure has been determined by historical interactions between climate, fire, livestock grazing, and logging. Climate change could substantially alter species abundance and composition, but the relative weight of the legacy of historical factors and projected future conditions in informing management objectives remains unresolved. We integrated land use histories with broad scale climatic factors to better understand how inland Pacific Northwest ecosystems may develop under projected climates. We measured vegetation structure and age distributions in five vegetation types (shrub steppe to subalpine forest) along an elevation gradient in the eastern Cascades of Washington. We quantitatively assessed compositional changes, and qualitatively summarized the environmental history (climate, fire and fire suppression, grazing, and logging) of each site. Little change was evident in woody species composition at the shrub steppe site. At the shrub steppe/forest ecotone, densities of drought-tolerant Artemisia tripartita and Pinus ponderosa increased. In the dry conifer, montane, and subalpine forest sites, increases in Pseudotsuga menziesii, Abies grandis, and Abies lasiocarpa, respectively, and decreases in Pinus ponderosa, Larix occidentalis, and Pinus contorta, respectively, have shifted species composition from fire and drought-tolerant species to shade-tolerant species. Fire suppression, grazing, and logging explain changes in species composition more clearly than climate variation does, although the relative influence of these factors varies with elevation. Furthermore, some of the observed changes in composition are opposite what we expect would be most suited to projected future climates. Natural resource managers need to recognize that the current state of an ecosystem reflects historical land uses, and that contemporary management actions can have long-term effects on ecosystem structure. Understanding the processes that generated an ecosystem's current structure will lead to more informed management decisions to effectively respond to projected climate changes.

Ten-year regeneration responses to varying levels of overstory retention in two productive southern British Columbia ecosystems

We investigated survival and growth responses of planted and advance natural regeneration species of varying shade tolerance to partial retention harvesting in moist warm Interior Cedar-Hemlock (ICHmw2) and dry cool Montane Spruce (MSdk) ecosystems of southeastern British Columbia, Canada. Treatments included three levels of overstory basal area retention (none, light (∼25%), or heavy (∼50%)) installed by two harvest methods (handfelled or a pushover falling technique being tested for its ability to control the spread of root disease). After 10 years, growth of both planted and natural regeneration species of varying shade tolerance tended to increase with decreasing overstory retention and associated increases in light availability. In contrast, significant survival responses to retention level were lacking except in the case of shade-intolerant western larch. Harvest method had a variable effect on regeneration survival and growth. Where significant responses did occur, they were generally attributed to harvesting effects on the characteristics of planting microsites rather than root disease spread. Natural regeneration densities at the ICHmw2 site were high at all retention levels, whereas stocking was less consistent at the MSdk site. We concluded that moderately shade-tolerant to shade-tolerant interior spruce and western redcedar can, under conditions similar to those of our study sites, be successfully established under overstories of up to approximately 25 m 2/ha basal area, but that growth performance is likely to be significantly lower than in clearcuts. Despite early survival issues, conclusions regarding Douglas-fir were similar. Poorer survival and vigour of shade-intolerant western larch suggested this species is not suitable for regeneration in partial retention systems where timber production is the primary objective. Where non-timber objectives predominate, survival and acceptable growth of even a small proportion of larch could add to the diversity of the regenerating stand.

Wood Changes in Four Size Classes of Fire-Killed Western Larch

Abstract Four size classes (8–12, 12.1–16, 16.1–20, and 20.1+ in. dbh) of fire-killed western larch (Larix occidentalis) were monitored and dissected over a 5-year period to assess causes and rates ofpostfire wood changes. Defect and merchantable volume were assessed by a certified scaler during the first 3 years. A greater proportion of wood volume in small trees was affected by decay, wood borers, and checks than in the large trees. Half of the 8‐12 in. dbh size class wood volumewas lost to postfire defects, whereas less than 15% of the 20.1+ in. dbh size class wood volume was lost to postfire defects after 3 years.

Ecological impacts and management strategies for western larch in the face of climate-change

Approximately 185,000 forest inventory and ecological plots from both USA and Canada were used to predict the contemporary distribution of western larch (Larix occidentalis Nutt.) from climate variables. The random forests algorithm, using an 8-variable model, produced an overall error rate of about 2.9 %, nearly all of which consisted of predicting presence at locations where the species was absent. Genetic variation among 143 populations within western larch’s natural distribution was predicted from multiple regression models using variables describing the climate of the seed source as predictors and response data from two separate genetic tests: 1) 15-year height at a field site in British Columbia, Canada, and, 2) two principal components of 8 variables describing growth, disease tolerance, and phenology of 6-year-old trees in a test in Idaho, USA. Presence and absence of the species and genetic variation within the species were projected into future climates provided by three General Circulation Models and two scenarios. Although the projections described pronounced impacts on the species and its populations, concurrence among the six projections pinpointed areas where the probability would be high that the future climate would be suitable for western larch. Concurrence among projections also was used to locate those sources of seed that should be best attuned genetically to future climates. The procedures outline a logical approach for developing management strategies for accommodating climate-change while taking into account the variability imposed by the differences among climatic estimates.

Occurrence and dominance of six Pacific Northwest conifer species

Questions: Can probability of occurrence and dominance be accurately estimated for six important conifer species with varying range sizes? Does range size impact the accuracy of species probability of occurrence models? Is species predicted probability of occurrence significantly related to observed dominance? Location: Pacific Northwest region, North America (601–401N, 1401–1101W). Methods: This study develops near range-wide predictive distribution maps for six important conifer species (Pseudotsuga menziesii, Tsuga heterophylla, Pinus contorta, Thuja plicata, Larix occidentalis, and Picea glauca) using forest inventory data collected across the United States and Canada. Species model accuracies are compared with range size using a rank scoring system. A suite of climate and topographic predictor variables are used to investigate environmental constraints that limit species range and quantify relationships between species predicted probability of occurrence and dominance at both plot and landscape scales. Results: Evaluation statistics revealed significant and accurate probability of occurrence models were developed for all six species. Based on ranked evaluation statistics, Tsuga heterophylla had highest overall model accuracy (statistic rank score 5 5) and Pinus contorta the lowest (statistic rank score 5 17). Across species, ranked evaluation statistics also revealed a pattern of decreasing model accuracy with increasing range size. At plot level, correlations between dominance and probability of occurrence were weakly positive for all species with only half of the species having statistically significant correlations. Pseudotsuga menziesii had the highest correlation (r 5 0.36, Po0.001) and Thuja plicata lowest (r 5 0.038, P 5 0.799). At the 50-km scale, correlations between dominance and probability of occurrence improved for all species except Pinus contorta. Pseudotsuga menziesii displayed the highest correlation (r 5 0.68, Po0.001) and Thuja plicata the lowest (r 5 0.07, P40.709). Conclusions: Species probability of occurrence model accuracy decreased with increasing range size. The strength and significance of correlations between probability of occurrence and dominance varied considerably by species and across spatial scales. Apart from Pseudotsuga menziesii and L. occidentalis, the results suggest that probability of occurrence is not a consistently reliable surrogate for species dominance in Pacific Northwest forests. We demonstrate how the degree of correlation between species occurrence and dominance can be used as an indicator of how well predictions of occurrence characterize the optimal niche of a species.

Carbon storage in old-growth and second growth fire-dependent western larch (Larix occidentalis Nutt.) forests of the Inland Northwest, USA

Abstract There is limited understanding of the carbon (C) storage capacity and overall ecological structure of old-growth forests of western Montana, leaving little ability to evaluate the role of old-growth forests in regional C cycles and ecosystem level C storage capacity. To investigate the difference in C storage between equivalent stands of contrasting age classes and management histories, we surveyed paired old-growth and second growth western larch (Larix occidentalis Nutt)–Douglas-fir (Pseudostuga menziesii var. glauca) stands in northwestern Montana. The specific objectives of this study were to: (1) estimate ecosystem C of old-growth and second growth western larch stands; (2) compare C storage of paired old-growth–second growth stands; and (3) assess differences in ecosystem function and structure between the two age classes, specifically measuring C associated with mineral soil, forest floor, coarse woody debris (CWD), understory, and overstory, as well as overall structure of vegetation. Stands were surveyed using a modified USFS FIA protocol, focusing on ecological components related to soil, forest floor, and overstory C. All downed wood, forest floor, and soil samples were then analyzed for total C and total nitrogen (N). Total ecosystem C in the old-growth forests was significantly greater than that in second growth forests, storing over 3 times the C. Average total mineral soil C was not significantly different in second growth stands compared to old-growth stands; however, total C of the forest floor was significantly greater in old-growth (23.8 Mg ha−1) compared to second growth stands (4.9 Mg ha−1). Overstory and coarse root biomass held the greatest differences in ecosystem C between the two stand types (old-growth, second growth), with nearly 7 times more C in old-growth trees than trees found on second growth stands (144.2 Mg ha−1 vs. 23.8 Mg ha−1). Total CWD on old-growth stands accounted for almost 19 times more C than CWD found in second growth stands. Soil bulk density was also significantly higher on second growth stands some 30+ years after harvest, demonstrating long-term impacts of harvest on soil. Results suggest ecological components specific to old-growth western larch forests, such as coarse root biomass, large amounts of CWD, and a thick forest floor layer are important contributors to long-term C storage within these ecosystems. This, combined with functional implications of contrasts in C distribution and dynamics, suggest that old-growth western larch/Douglas-fir forests are both functionally and structurally distinctive from their second growth counterparts.

Assessing the Threat Posed by Indigenous Exotics: A Case Study of Two North American Bark Beetle Species

ABSTRACT The Douglas-fir beetle, Dendroctonus pseudotsugae Hopkins, was detected in 2001 in northern Minnesota outside its natural range and the range of its native hosts, Douglas-fir, Pseudotsuga menziesii (Mirbel) Franco, and western larch, Larix occidentalis Nutt. Consecutive years of detection indicated that D. pseudotsugae may have been established in a new environment and provided a possible example of an indigenous exotic species in North America. Pheromone-baited logs of P. menziesii and tamarack, Larix laricina (Du Roi) K. Koch, were placed at four sites in northern Minnesota in an attempt to detect this indigenous exotic. Bark was removed from one half of logs and checked for D. pseudotsugae galleries. The remaining logs were left intact and reared to collect adult beetles the following spring. No D. pseudotsugae galleries were identified and no adults of the indigenous exotic were located. Along with the eastern larch beetle, Dendroctonus simplex LeConte, native Minnesota populations of Polygra...

Characteristics and densities of Williamson’s Sapsucker nest trees in British Columbia

We characterized Williamson’s Sapsucker ( Sphyrapicus thyroideus (Cassin, 1852)) nest trees in southern British Columbia from 1995 to 2008 to determine the nesting requirements of this endangered species in Canada. In the East Kootenay (n = 32) and the Okanagan (n = 157) regions where western larch ( Larix occidentalis Nutt.) occurred, 81% and 75% of the nest trees were western larch, respectively. In regions west of the Okanagan Valley where western larch did not occur (n = 73), 77% of the nest trees were trembling aspen ( Populus tremuloides Michx.). Conifer nest trees were larger (72 cm DBH), on average, than broad-leaved nest trees (35 cm DBH), and this pattern was consistent across the breeding range of Williamson’s Sapsucker. Live western larch >67 cm DBH with either broken or dead tops were the trees with the highest frequency (20%) among the trees estimated to be suitable for nesting, primarily because these larch tended to be infected with heart rots. Based on observed densities, targets should be 1.36 suitable nest trees/ha in managed stands, although in many instances such as aspen patches, this target will only be met in portions of the nesting territories. The recommended stand structure for long-term maintenance of high nest-tree density in western larch stands is for ≥20 trees/ha with DBHs >57 cm and 60–150 trees/ha with DBHs >22 cm.

Fall Planting and Tree Shelters for Reforestation in the East Washington Cascades

Abstract Reforestation on harsh, high-elevation sites near the crest of the Cascade Mountains in Washington can be challenging because of persistent snowpack and extreme climatic variation. The use of tree shelters was investigated with two species, Douglas-fir (Pseudotsuga menziesii [Mirb.] Franco) and western larch (Larix occidentalis Nutt.), on two Yakama Nation sites across three fall planting dates. For both species, seedlings inside tree shelters had increased survival compared with nonsheltered seedlings after one growing season despite increased damage by crushing from snowpack. Volumetric soil moisture content on the western larch site increased from 10% to 17% between the first and second planting dates, resulting in no effect of planting date on western larch survival. On the Douglas-fir site, however, soil moisture content was below 12% for all fall planting dates, resulting in only 2% survival for those seedlings planted on the first planting date. First-season seedling growth was unaffected by planting date or tree shelter treatment for both species. These results indicate the critical importance of soil moisture at time of planting and onset of precipitation after planting. Use of tree shelters may improve seedling survival on a harsh, high-elevation site, but it increases potential seedling damage because of crushing by snowpack.

Tree species identification in mixed coniferous forest using airborne laser scanning

This study tests the capacity of relatively low density (<1 return/m 2) airborne laser scanner data for discriminating between Douglas-fir, western larch, ponderosa pine, and lodgepole pine in a western North American montane forest and it evaluates the relative importance of intensity, height, and return type metrics for classifying tree species. Collectively, Exploratory Data Analysis, Pearson Correlation, ANOVA, and Linear Discriminant Analysis show that structural and intensity characteristics generated from LIDAR data are useful for classifying species at dominant and individual tree levels in multi-aged, mixed conifer forests. Proportions of return types and mean intensities are significantly different between species ( p -value < 0.001) for plot-level dominant species and individual trees. Classification accuracies based on single variables range from 49%–61% at the dominant species level and 37%–52% for individual trees. The accuracy can be improved to 95% and 68% respectively by using multiple variables. The inclusion of proportion of return type greatly improves the classification accuracy at the dominant species level, but not for individual trees, while canopy height improves the accuracy at both levels. Overall differences in intensity and return type between species largely reflect variations in the physical structure of trees and stands. These results are consistent with the findings of others and point to airborne laser scanning as a useful source of data for species classification. However, there are still many knowledge gaps that prevent accurate mapping of species using ALS data alone, particularly with relatively sparse datasets like the one used in this study. Further investigations using other datasets in different forest types will likely result in improvements to species identification and mapping for some time to come.

Optimization of combined genetic gain and diversity for collection and deployment of seed orchard crops

Genetic gain and diversity of seed orchards’ crops are determined by the number of parents, their breeding values and relatedness, within-orchard pollination efficiency, and level of pollen contamination. These parameters can be manipulated at establishment by varying clonal representation (e.g., linear deployment), during orchard development by genetic thinning, or by selective harvesting. Since clonal fecundities are known to vary both within and among years, then each seed crop has a unique genetic composition and, therefore, crops should be treated on a yearly basis. Here we present an optimization protocol that maximizes crop’s genetic gain at any desired genetic diversity through the selection of a subset of the crop that meets both parameters. The genetic gain is maximized within the biological limit set by each clone’s seed-cone production and effective population size is used as a proxy to genetic diversity whereby any relationship among clones is considered. The optimization was illustrated using 3 years’ reproductive output data from a first-generation western larch seed orchard and was tested under various scenarios including actual male and female reproductive output and male reproductive output assumed to be either equal to that of female or a function of clonal representation. Furthermore, various levels of co-ancestry were assigned to the orchard’s clones in supplementary simulations. Following the optimization, all solutions were effective in creating custom seedlots with different gain and diversity levels and provided the means to estimate the genetic properties of composite seedlots encompassing the remaining “unused” seed from a number of years.

Development and characterization of microsatellite loci in western larch (Larix occidentalis Nutt.)

Western larch (Larix occidentalis Nutt.) is an important ecological and commercial species in the Pacific Northwest. We isolated nine microsatellite loci with variable polymorphism ranging from five to 19 alleles per locus. Observed and expected heterozygosities averaged 0.42 and 0.64 and ranged from 0.11 to 0.83 and from 0.48 to 0.80, respectively. These markers, along with those already existing, will be useful for the species' gene resource management activities.

The fire history of a 416-year-old western larch tree in southeastern British Columbia

The fire history obtained from pre-settlement fire-scarred trees provides useful benchmarks for the restoration of dry interior, fire-dependent forest ecosystems. In the Rocky Mountain Trench of southeast British Columbia, historical benchmarks obtained from periods prior to significant European influence (i.e., pre-1850) are common reference points for forest ecosystem restoration. This extension note discusses the fire history of a 416-year-old western larch (Larix occidentalis) whose growth rings recorded 268 years of fire history before 1850. The study tree's estimated mean fire interval (MFI) is 34.1 years, and its fire intervals ranged from 19 years to 51 years. The tree did not record a fire during the last 130 years of its life. This extension note also discusses the development of a network of cross-dated benchmark sites across the landscape that would create a master fire chronology for the region. Such a chronology would reflect the natural variability of historical fire patterns, helping policy-makers, managers, recovery teams, and restoration practitioners understand the spatial and temporal distribution of landscape-scale disturbances that historically have created fire-dependent ecological communities (many of which include wildlife and plant species now at risk). The creation of a benchmark network would also refine dry interior forest restoration/conservation programs and improve their efficacy.