Background: Incidence of tooth size discrepancy varies between different racial and ethnic groups. Values of tooth size ratios for Punjab population were found to be different when compared to Bolton's Caucasian population. Materials and Methods: The study sample consisted of study casts of 300 subjects (150 males; 150 females) with an age range of 12-14 years of Punjab population. Alginate Impressions of both arches were taken and dental casts were made. Mesiodistal dimension of each tooth was measured with the help of Digital Vernier Caliper. Further, mean anterior ratio (AR) and overall ratio (OR) was calculated. Results: AR was 80.87% for males and 78.77% for females. OR was 93.93% in males and 91.58% in females. Statistically significant difference was found between tooth size ratios of Punjab and Caucasian population. Males had larger tooth size ratios as compared to females. Conclusion: These results suggest that tooth size relationships are population specific. Values specific to specific population should be derived.