Large Woody Research Articles

Using continuous surveys to evaluate precision and bias of inferences from design-based reach-scale sampling of stream habitat

Accurately estimating stream channel characteristics is essential for managing and restoring populations and aquatic ecosystems. Reach-based sampling designs have been used extensively to collect fisheries related data; however, few studies have examined accuracy and precision of scaling up reach-based sampling designs to stream habitat assessments. Here, we used continuous habitat surveys across multiple reaches to census stream attributes in tributaries in the upper Lewis River, Washington and better understand the potential bias and precision of reach-based designs. We used these continuous data to create simulated outcomes from three different random sampling designs. We found precision of estimates of stream-level habitat attributes (large woody debris, residual pool depth, and grain size) increased with the number of reaches sampled (i.e., sampling intensity); however, effort needed to achieve reasonable precision (coefficient of variation = 0.20) varied across streams, attributes, and designs. Bias (i.e., estimate—the truth) was relatively low, but also varied across streams and attributes. Our findings illustrate the challenges of using reach-based designs for stream-level habitat assessments and the need for novel approaches for broader data collection.

Genome sequencing and comparative analysis of Ficus benghalensis and Ficus religiosa species reveal evolutionary mechanisms of longevity

SummaryFicus benghalensis and Ficus religiosa are large woody trees well known for their long lifespan, ecological and traditional significance, and medicinal properties. To understand the genomic and evolutionary aspects of these characteristics, the whole genomes of these Ficus species were sequenced using 10x Genomics linked reads and Oxford Nanopore long reads. The draft genomes of F. benghalensis and F. religiosa comprised of 392.89 Mbp and 332.97 Mbp, respectively. We established the genome-wide phylogenetic positions of the two Ficus species with respect to 50 other Angiosperm species. Comparative evolutionary analyses with other phylogenetically closer Eudicot species revealed adaptive evolution in genes involved in key cellular mechanisms associated with prolonged survival including phytohormones signaling, senescence, disease resistance, and abiotic stress tolerance, which provide genomic insights into the mechanisms conferring longevity and suggest that longevity is a multifaceted phenomenon. This study also provides clues on the existence of CAM pathway in these Ficus species.

PING‐Mapper: Open‐Source Software for Automated Benthic Imaging and Mapping Using Recreation‐Grade Sonar

AbstractThe characterization of benthic habitats is essential for aquatic ecosystem science and management, but is frequently limited by waterbody visibility and depth. Recreation‐grade side‐scan sonar systems are increasingly used to aid scientific inquiries in aquatic environments due to their relative low‐cost, ease of operation, low‐weight, and ease of mounting on a variety of vessels. However, existing procedures and software for post‐processing these data are either limited, closed source, or fail on data from new sonar models, limiting the development of reproducible workflows. Here, we present PING‐Mapper, an open‐source and freely available side‐scan sonar post‐processing toolset for processing and mapping sonar recordings from popular Humminbird instruments. The modular software automatically: (a) decodes sonar recordings from any Humminbird system, (b) exports ping attributes from every sonar channel, (c) uses sonar sensor depth for water column removal, and (d) exports sonogram tiles and georectified mosaics. Sonar channels are processed in parallel for quick decoding and metadata extraction. Major processing wokflows, including georectification and image export, are optimized to scale with computing resources. The software has been extensively tested using data from several rivers of varying character and distribution of depths, but could also be used in estuarine and lacustrine environments. Usage of PING‐Mapper is illustrated in three case studies focused on mapping large woody debris, bathymetric mapping, and visual interpretation and mapping of substrates for selected reaches of the Pearl and Pascagoula river systems in Mississippi.

Transcriptome Analysis of Moso Bamboo (Phyllostachys edulis) Reveals Candidate Genes Involved in Response to Dehydration and Cold Stresses.

Bamboo (Bambusoideae) belongs to the grass family (Poaceae) and has been utilized as one of the most important nontimber forest resources in the world. Moso bamboo (Phyllostachys edulis) is a large woody bamboo with high ecological and economic values. Global climate change brings potential challenges to the normal growth of moso bamboo, and hence its production. Despite the release of moso bamboo genome sequence, the knowledge on genome-wide responses to abiotic stress is still limited. In this study, we generated a transcriptome data set with respect to dehydration and cold responses of moso bamboo using RNA-seq technology. The differentially expressed genes (DEGs) under treatments of dehydration and cold stresses were identified. By combining comprehensive gene ontology (GO) analysis, time-series analysis, and co-expression analysis, candidate genes involved in dehydration and cold responses were identified, which encode abscisic acid (ABA)/water deficit stress (WDS)-induced protein, late embryogenesis abundant (LEA) protein, 9-cis-epoxycarotenoid dioxygenase (NCED), anti-oxidation enzymes, transcription factors, etc. Additionally, we used PeLEA14, a dehydration-induced gene encoding an “atypical” LEA protein, as an example to validate the function of the identified stress-related gene in tolerance to abiotic stresses, such as drought and salt. In this study, we provided a valuable genomic resource for future excavation of key genes involved in abiotic stress responses and genetic improvement of moso bamboo to meet the requirement for environmental resilience and sustainable production.

Experimental and Numerical Investigation of Floating Large Woody Debris Impact on a Masonry Arch Bridge

Masonry arch bridges form an essential part of existing transport infrastructure around the world, including mainland Europe and the northeastern US. Recent extreme flood events highlight that masonry arch bridges spanning watercourses are vulnerable to flood-induced hydrodynamic and debris impact loads. When the flow interacts with the bridge superstructure, with or without discrete floating debris, a complex interaction is observed. This paper presents both experimental and numerical studies to investigate this complex phenomenon, including fluid–structure and structure–structure interactions. A typical single-span masonry arch bridge and large woody debris representing a tree log are investigated. Experimental observations from a scaled hydraulic model, with and without debris in the flow, are first presented for the case where the abutment of the bridge is fully submerged. Next, the capability of the numerical method smoothed particle hydrodynamics (SPH) in simulating the hydrodynamic behaviour and debris impact observed in the experiment is discussed. Following this, both hydrodynamic and debris-induced pressure–time histories on the bridge are obtained using the SPH model. Results reveal that the debris impact leads to a significantly more localised load on the bridge compared to the situation with hydrodynamic load only.

LARGE WOODY DEBRIS ACCUMULATIONS IN THE LATE PENNSYLVANIAN TROPICS—EVOLUTIONARY SIGNAL OR TECTONO-CLIMATIC ARCHIVE?

ABSTRACTBy colonizing drylands, plants fundamentally changed continental deposition and, thus, intensified the interaction between life and sediments. Fossil large woody debris in epiclastic strata is a key archive of this environmental turnover, although its interpretation remains challenging due to taphonomic biases. We review voluminous fluvial red-bed successions with sizeable silicified trunks that characterize Middle Pennsylvanian–lower Permian strata of east-central Europe. The stratigraphic occurrence, petrography, architecture of the deposits, and the preservation and nature of the fossil wood are discussed in the context of the tectono-climatic and vegetational evolution of the central-Pangean low latitudes. The log-bearing successions are assigned to five distinct, regionally traceable stratigraphic levels between the middle Moscovian and early Asselian. Up to 20 m long, mostly decorticated trunk fragments occur isolated in more or less feldspathic channel deposits, the architectures and dimensions of which point to large-scale river systems with highly variable discharge. Wood anatomy and floodplain adpression-fossils show that the trunks were derived from cordaitaleans, conifers, and arborescent sphenopsids in more diverse, gymnosperm-dominated dryland floras. The fossil record is biased towards successions formed in large-catchment river systems and, thus, does not accurately document the genuine nature of plant-distribution patterns. Rather, the strata show that large woody debris preservation depended on fluvial style and hydrological regime, hence turning the woody deposits into climate archives. The strata elucidate the climate development in equatorial Pangea, paralleling the acme of the Late Paleozoic Ice Age.

Biodegraders of Large Woody Debris Across a Tidal Gradient in an Indonesian Mangrove Ecosystem

There has been limited research on the breakdown, recycling, and flux of carbon from large woody detritus (LWD) in mangrove forests. The breakdown of LWD is caused by guilds of terrestrial and marine biodegrading organisms that degrade wood at a range of rates and efficiencies. Spatial variations in environmental factors within mangroves affect the distribution and community of biodegrading organisms, which, in turn, impacts carbon flow and sequestration. We reveal the role of biodegrading organisms in LWD breakdown and the environmental factors that influence the distribution of biodegrading guilds within a mangrove forest in South East Sulawesi that supports a diversity of mangrove species typical of Indonesian mangrove forests, which constitute 20% of Global mangrove cover. Within the high intertidal regions, terrestrial biodegradation processes dominated upon LWD. After 12 months exposure on the forest floor, experimental wooden panels in these areas remained unchanged in mass and condition. In the low intertidal region, marine wood-boring animals belonging to the family Teredinidae were the dominant biodegraders of LWD, and their activity reduces LWD volume and speeds up the loss of LWD volume. More than 50% of the experimental wooden panels’ weight in these areas was lost after 12 months exposure on the forest floor. Although different biodegrading guilds occupy the same LWD niche, their distribution throughout the mangrove forest is influenced by inundation time. The change of biodegrading guilds within LWD between the terrestrial and the marine organisms was distinct, creating a biodegradation boundary in a distance as narrow as 1 m on the mangrove forest floor. These results are important, as rising sea levels have crucial implications for biodegrading guilds. A full understanding of factors affecting the biodegradation processes of LWD in mangrove forests is critical to accurately assess mangrove carbon stores and the fate of mangrove derived carbon.

Microhabitat Characteristics and Management of the Trinity Bristle Snail in the Greater Trinity Basin of Northern California

Microhabitat assessment of the Trinity bristle snail (Monadenia setosa), a state threatened species, was conducted at 88 randomly selected sites throughout its known geographic range in northern California. Nineteen abiotic and biotic environmental variables were measured for each site. Results of univariate and multivariate analyses indicate that sample sites were dominated by physical parameters of air and soil temperature, and elevation and exposure in association with habitat structure consisting of the presence, size, and nearness of large woody debris, rocky surface and subsurface structure, and riparian stream corridors, respectively. No individual or small suite of attributes defined microhabitat suitability for the species based on site-specific characteristics. Instead, a robust combination of physical and biological variables was key to the distribution of specimens at the population-level, most of which were allied with structural elements of the sample site.

Blockage of box-shaped and circular culverts under flood event conditions: a laboratory investigation

ABSTRACT Culverts are used to allow runoff to pass through roads, railways, and embankments. Accumulation of debris during flood events reduces the culvert flow capacity and hence flow overtopping results in culvert failure both hydraulically and structurally. This paper presents the results of an experimental study of temporal variations of blockage upstream of culverts due to woody debris under unsteady flow conditions. To simulate flood conditions, a synthetic flow hydrograph was produced in the laboratory. Cylindrical wooden dowels with two different diameters were used to simulate the woody debris carrying during flood events. Two culvert shapes including box and circular pipe culverts are examined here. The results showed that the maximum percentage of blockage occurs during the falling limb of the hydrograph. Although the feeding rate of smaller diameter woody debris into the flow is of considerable importance in the culvert blockage, the blockage percentage is not influenced by the feeding rate of large woody debris. It was also found that the pipe culvert is more susceptible to blockage than the box-shaped culvert. Using regression analysis, predictive equations are suggested to estimate the percentage of culvert blockage during flood events.

Generation and Subsequent Transport of Landslide-driven Large Woody Debris Induced by the 2018 Hokkaido Eastern Iburi Earthquake

The earthquake that occurred on 6 September 2018, in the eastern part of the Iburi region of Hokkaido, Japan (the Hokkaido Eastern Iburi Earthquake) caused thousands of shallow landslides in mountain areas. In areas where many landslides occurred, the trees on the slope became large woody debris (LWD) and were supplied to the catchment. Understanding the properties of LWD during the earthquake and its subsequent movement after the earthquake are important to manage the produced LWD and implement disaster prevention measures. This study evaluates the risk of future LWD disasters based on the sequence of LWD generation, its spatial distribution, and LWD relocation linked to temporal fluctuations in rainfall events. The study site is the upper Habiu River catchment (0.37 km2), where multiple shallow earthquake-related landslides occurred. Orthophotos and elevation data acquired before and after the earthquake were used to detect the properties of LWD. To evaluate the risk of an LWD disaster, we examined the correspondence between the hydraulic quantities, including the precipitation for 2 years after the earthquake and the water depth. It was estimated that approximately 7,000 LWD pieces (9,119 m3 km−2) were produced during the earthquake. Orthophoto interpretations indicate that over 80% of the LWD produced at the time of the landslide moved from the slope to the channel accompanied by the landslide debris; some of that then flowed down, accumulated, and formed logjams. In the river channel approximately two years after the earthquake, the destruction of logjams and the clear and drastic movement of LWD could not be confirmed. In this catchment, the uneven LWD distribution and the formation of logjams were fixed almost immediately after the landslide at the time of the earthquake; these characteristics are important when considering future actions. The water depth evaluation based on the difference in the excess return period indicate that the degree of risk differs depending on the deposition location in the channel. This suggests that not all LWD in the catchment are dangerous and that a risk assessment focusing on the LWD location can be effective. This study also makes it possible to determine high priority areas for LWD treatment.

The Evaluation of Color Spaces for Large Woody Debris Detection in Rivers Using XGBoost Algorithm

Large woody debris (LWD) strongly influences river systems, especially in forested and mountainous catchments. In Taiwan, LWD are mainly from typhoons and extreme torrential events. To effectively manage the LWD, it is necessary to conduct regular surveys on river systems. Simple, low cost, and accurate tools are therefore necessary. The proposed methodology applies image processing and machine learning (XGBoost classifier) to quantify LWD distribution, location, and volume in river channels. XGBoost algorithm was selected due to its scalability and faster execution speeds. Nishueibei River, located in Taitung County, was used as the area of investigation. Unmanned aerial vehicles (UAVs) were used to capture the terrain and LWD. Structure from Motion (SfM) was used to build high-resolution orthophotos and digital elevation models (DEM), after which machine learning and different color spaces were used to recognize LWD. Finally, the volume of LWD in the river was estimated. The findings show that RGB color space as LWD recognition factor suffers serious collinearity problems, and it is easy to lose some LWD information; thus, it is not suitable for LWD recognition. On the contrary, the combination of different factors in different color spaces enhances the results, and most of the factors are related to the YCbCr color space. The CbCr factor in the YCbCr color space was best for identifying LWD. LWD volume was then estimated from the identified LWD using manual, field, and automatic measurements. The results indicate that the manual measurement method was the best (R2 = 0.88) to identify field LWD volume. Moreover, automatic measurement (R2 = 0.72) can also obtain LWD volume to save time and workforce.

Effects of In-Channel Structure on Chinook Salmon Spawning Habitat and Embryo Production

Adult salmonids are frequently observed building redds adjacent to in-channel structure, including boulders and large woody debris. These areas are thought to be preferentially selected for a variety of reasons, including energy and/or predation refugia for spawners, and increased hyporheic exchange for incubating embryos. This research sought to quantify in-channel structure effects on local hydraulics and hyporheic flow and provide a mechanistic link between these changes and the survival, development, and growth of Chinook salmon Oncorhynchus tshawytscha embryos. Data were collected in an eight-kilometer reach, on the regulated lower Mokelumne River, in the California Central Valley. Nine paired sites, consisting of an area containing in-channel structure paired with an adjacent area lacking in-channel structure, were evaluated. Results indicated that in-channel structure disrupts surface water velocity patterns, creating pressure differences that significantly increase vertical hydraulic gradients within the subsurface. Overall, in-channel structure did not significantly increase survival, development, and growth of Chinook salmon embryos. However, at several low gradient downstream sites containing in-channel structure, embryo survival, development, and growth were significantly higher relative to paired sites lacking such features. Preliminary data indicate that adding or maintaining in-channel structure, including woody material, in suboptimal spawning reaches improves the incubation environment for salmonid embryos in regulated reaches of a lowland stream. More research examining temporal variation and a full range of incubation depths is needed to further assess these findings.

Vital Parameters Assessments of Starvation Tolerance of in vitro Populus alba Culture

Populus alba is a large woody deciduous plant. The plant has been introduced to shooting, then multiplication of rooting on Murashige and Skoog (MS) medium. This work was designed to estimate the effect of two factors (low levels of 1-Naphthaleneacetic acid NAA and sucrose) on P. alba response resulting in 6 treatments compared to the control, with twelve measured responses. There was a significant difference in some measurements in morphology, like plantlets fresh-weight, shoot-, root-length, and leaf number. In the physiological measurements, there were significant differences in all the measured parameters. The low concentrations of sucrose and media composition/power (MS grams/L) led to starvation in plants; however, these conditions led to enhancement in some morphological and physiological parameters to overcome the starvation effect, compared to the control. The RAPD-PCR molecular marker (four decamers) was used to evaluate the new individuals’ genetic variation (instability), resulting in a total polymorphism percentage of 50.83%. It was formerly known that the plantlets were identical to each other and to the mother plant. In this study, however, the use of distinct media power, hormonal and sucrose levels resulted in molecular variation reflected in P. alba’s morphological and physiological responses.

Identifying Fagaceae and Lauraceae species using leaf images and convolutional neural networks

Lauraceae and Fagaceae are two large woody plant families that are predominant in the low- and middle-altitude regions in Taiwan. The highly interspecific similarity between some species of the family brings limitations on the management and utilization. This work proposed an approach for identifying 15 Lauraceae species and 20 Fagaceae species using leaf images and convolutional neural networks (CNNs). Leaf specimens of 35 species were collected from the northern, central, and southern parts of Taiwan. Images of the leaves were acquired using flat-bed scanners. Three CNN architectures—DenseNet-121, MobileNet V2, and Xception—were trained. Xception achieved the highest mean test accuracy of 99.39%, and MobileNet V2 required the shortest mean test time of 17.1 ms per image using a GPU. The saliency maps revealed that the characteristics learned by models matched the leaf features used by botanists. A pruning algorithm, gate decorator, was applied to the trained models for reducing the number of parameters and number of floating-point operations of the MobileNet V2 by 55.4% and 69.1%, respectively, while the model accuracy was maintained at 92.03%. Thus, MobileNet V2 has the potential to be used for identifying the Lauraceae and Fagaceae species on mobile devices.

Greening at multiple scales promote biodiverse cities: A multi-scale assessment of drivers of Neotropical birds

Biodiversity-sensitive cities can contribute to reconnect humans with nature and halt global biodiversity loss. Achieving biodiversity-sensitive cities is challenging, especially in regions threatened by growing urbanization. To inform urban management and planning in a global biodiversity hotspot, we conducted a multi-scale assessment of drivers of Neotropical birds in the capital city of Santiago de Chile. We investigate the influence of local and landscape variables on native bird species richness and abundance. We surveyed birds and vegetation in 449 sampling points distributed across the city. Native bird species richness was greater in areas with greater shrub and woody vegetation cover at the local scale. Native bird species richness was also greater with high vegetation density in the surrounding landscape and near to an urban boundary. Native birds were abundant in areas with large woody vegetation cover at both local and landscape scales, high vegetation density in the surrounding landscape, near to an urban park and near to an urban boundary. Additive effects of vegetation at different spatial scales suggest that combining local and landscape management, planning and design will be best to preserve native birds in a large city. Although native birds are species rich and abundant near the urban fringe and decrease towards the interior of the city, local-scale management of habitat encouraging shrub and tree planting and landscape-scale actions such as targeting high levels of vegetation (including woody cover) and providing a well-distributed network of urban parks will help sustain native birds across the city. Greening actions at local and landscape-scale will contribute to achieving biodiversity-sensitive cities, providing benefits for people and nature.

Recent Updates in Research on Gymnema sylvestre

Gymnema sylvestrae (GS) is a large woody climbing plant that is available in dry forests of India and China. It is also proven for other activities like anti-hemolytic, anti-bacterial and anti-cancer. Present review covers recent updates in research on this plant typically during last 10 years. Also, its characterization and extraction, structure activity relationship, in-vitro pharmacology, in-vivo pharmacology, pharmacodynamics, clinical pharmacology and clinical efficacy, safety and toxicity is discussed in details. Detailed review of available literature on GS indicates it is promising plant for treatment of Type 2 Diabetes Mellitus. GS acts only in presence of pancreas as it acts by stimulation of β cells of langerhans. However, it has also shown hepatotoxic property.

Retention and Dimensional Changes of Evergreen Brush Piles Within a Flood Control Reservoir

Abstract Brush piles (i.e., trees and large woody debris) are often installed in reservoirs to supplement fish habitat. The retention and dimensional change of brush piles after installation is important information that can be used to maximize the effectiveness of this management action. We evaluated the retention and dimensional change of 70 eastern red cedar Juniperus virginiana and bald cypress Taxodium distichum brush piles in an embayment of a drawdown reservoir up to four annual cycles of submergence and exposure. We used satellite imagery to supplement our onsite measurements of retention. We also examined spatial patterns of brush pile retention and dimensional change. Brush piles were lost at 10% per year, and their volume was lost at 14% per year. We compared our rates of brush pile retention and dimensional change with those from a holdout data set of 50 brush piles. Estimates between data sets did not differ statistically. Spatial patterns of retention and dimensional change coincided with morphological features in our study area, suggesting that retention and dimensional change is influenced by variable physical forces (e.g., wave action and flow) at installation locations. Our estimates of brush pile retention and dimensional change can be used to generally sustain desirable brush densities. For example, to maintain a fixed total volume of brush in our study embayment, roughly 23% of the total brush volume installed would need to be replaced annually. Similar research in reservoirs managed for other purposes is needed, as length and cycle of inundation could lead to variable rates of retention and dimensional change. Additionally, advancements into computer-assisted detection and volume estimation could reduce the time and effort needed to monitor brush piles.

Landscape Structure of Woody Cover Patches for Endangered Ocelots in Southern Texas

Few ecological studies have explored landscape suitability using the gradient concept of landscape structure for wildlife species. Identification of conditions influencing the landscape ecology of endangered species allows for development of more robust recovery strategies. Our objectives were to (i) identify the range of landscape metrics (i.e., mean patch area; patch and edge densities; percent land cover; shape, aggregation, and largest patch indices) associated with woody vegetation used by ocelots (Leopardus pardalis), and (ii) quantify the potential distribution of suitable woody cover for ocelots across southern Texas. We used the gradient concept of landscape structure and the theory of slack combined with GPS telemetry data from 10 ocelots. Spatial distribution of high suitable woody cover is comprised of large patches, with low shape-index values (1.07–2.25), patch (27.21–72.50 patches/100 ha), and edge (0–191.50 m/ha) densities. High suitability landscape structure for ocelots occurs in 45.27% of woody cover in southern Texas. Our study demonstrates a new approach for measuring landscape suitability for ocelots in southern Texas. The range of landscape values identified that there are more large woody patches containing the spatial structure used by ocelots than previously suspected, which will aid in evaluating recovery and road planning efforts.

A comparison between TLS and UAS LiDAR to represent eucalypt crown fuel characteristics

Advances in fire behaviour modelling provide a catalyst for the development of next generation fuel inputs. Fire simulations underpin risk and consequence mapping and inform decisions regarding ecological and social impacts of different fire regimes. Unoccupied Aerial Systems (UAS) carrying Light Detection and Ranging (LiDAR) sensors have been proposed as a source of structural information with potential for describing fine fuel properties. Whilst these systems have been shown to be capable of describing general vegetation distribution, the ability to distinguish between vegetation elements that contribute to fire spread and those that do not (such as large woody elements) is yet to be explored. This study evaluates the ability of UAS LiDAR point clouds to provide a description of crown fuel elements in eucalypt trees. This is achieved through comparison with dense Terrestrial Laser Scanning (TLS) that were manually attributed with a fuel description. Using the TLSeparation package TLS and UAS LiDAR point clouds achieved 84.6% and 81.1% overall accuracy respectively in the separation of crown fuel and wood in nine reference trees. When applying the same separation process across a 30 by 50 m plot consisting of approximately 75 trees, total canopy fuel volume was found to be strongly correlated between the TLS and UAS LiDAR point clouds (r: 0.96, RMSE: 1.53 m3). A lower canopy base height and greater distance between crown fuel regions within each crown supported visual inspection of the point clouds that TLS point clouds were able to represent the crown to a greater extent than UAS LiDAR point clouds. Despite these differences it is likely that a less complete representation of canopy fuel such as that generated from UAS LiDAR point clouds will suitably represent the crown and canopy fuel objects effectively for fire behaviour modelling purposes. The research presented in this manuscript highlights the potential of TLS and UAS LiDAR point clouds to provide repeatable, accurate 3D characterisation of canopy fuel properties.

WOODY VEGETATION ENCROACHMENT: A DRIVER OF HERBACEOUS SPECIES DIVERSITY LOSS IN A COASTAL FEN

Early successional wetland habitat is being lost in temperate regions worldwide as a result of changes in disturbance regimes that allow for the establishment and dominance of woody species. This phenomenon is pronounced in fens, which harbor high numbers of special status plant species that require early successional habitat. We investigated the relationship between woody vegetation encroachment and herbaceous species richness within a northern California coastal fen that has been undergoing encroachment by woody vegetation for ca. 80 years. We established 25 transects within the fen and sampled 338 1x1 m plots located at 4-m intervals along transects. At each plot, we recorded the cover of all woody and herbaceous species, as well as litter, thatch, open water, bare soil, and cover of large woody debris. Modeling indicated that woody vegetation cover, height, species richness, litter cover, and distance from the edge of fen were significant predictors of herbaceous species richness. Vegetation sampling indicated that herbaceous species richness declined with increased woody vegetation cover and height and increased litter cover. Canopy closure was found to result in the complete loss of rare plant species and a significant reduction in herbaceous species richness. The results of this study suggest that the re-introduction of disturbance, specifically those that result in the removal of woody vegetation and litter accumulation, is probably essential to maintain herbaceous species diversity and persistence of special status plant species populations in coastal fens.