The low specific speed centrifugal pump plays a crucial role in industrial applications, and ensuring its efficient and stable operation is extremely important for the safety of the whole system. The pump must operate with an extremely high head, an extremely low flow rate, and a very fast speed. The internal flow structure is complex and there is a strong interaction between dynamic and static components; consequently, the hydraulic excitation force produced becomes a significant factor that triggers abnormal vibrations in the pump. Therefore, this study focuses on a low specific speed centrifugal pump and uses a single-stage model pump to conduct PIV and pressure pulsation tests. The findings reveal that the PIV tests successfully captured the typical jet-wake structure at the outlet of the impeller, as well as the flow separation structure at the leading edge of the guide vanes and the suction surface. On the left side of the discharge pipe, large-scale flow separation and reverse flow happen as a result of the flow-through effect, producing a strong vortex zone. The flow field on the left side of the pressure chamber is relatively uniform, and the low-speed region on the suction surface of the guide vanes is reduced due to the reverse flow. The results of the pressure pulsation test showed that the energy of pressure pulsation in the flow passage of the guide vane occurs at the fBPF and its harmonics, and the interaction between the rotor and stator is significant. Under the same operating condition, the RMS value distribution and amplitude at fBPF of each measurement point are asymmetric in the circumferential direction. The amplitude of fBPF near the discharge pipe is lower, while the RMS value is higher. A complex flow structure is shown by the larger amplitude and RMS value of the fBPF on the left side of the pressure chamber. With the flow rate increasing, the energy at fBPF of each measurement point increases first and then decreases, while the RMS value decreases, indicating a more uniform flow field inside the pump.