Large-scale Collection Research Articles

Approval-Based Committee Voting in Practice: A Case Study of (over-)Representation in the Polkadot Blockchain

We provide the first large-scale data collection of real-world approval-based committee elections. These elections have been conducted on the Polkadot blockchain as part of their Nominated Proof-of-Stake mechanism and contain around one thousand candidates and tens of thousands of (weighted) voters each. We conduct an in-depth study of application-relevant questions, including a quantitative and qualitative analysis of the outcomes returned by different voting rules. Besides considering proportionality measures that are standard in the multiwinner voting literature, we pay particular attention to less-studied measures of overrepresentation, as these are closely related to the security of the Polkadot network. We also analyze how different design decisions such as the committee size affect the examined measures.

InstructDoc: A Dataset for Zero-Shot Generalization of Visual Document Understanding with Instructions

We study the problem of completing various visual document understanding (VDU) tasks, e.g., question answering and information extraction, on real-world documents through human-written instructions. To this end, we propose InstructDoc, the first large-scale collection of 30 publicly available VDU datasets, each with diverse instructions in a unified format, which covers a wide range of 12 tasks and includes open document types/formats. Furthermore, to enhance the generalization performance on VDU tasks, we design a new instruction-based document reading and understanding model, InstructDr, that connects document images, image encoders, and large language models (LLMs) through a trainable bridging module. Experiments demonstrate that InstructDr can effectively adapt to new VDU datasets, tasks, and domains via given instructions and outperforms existing multimodal LLMs and ChatGPT without specific training.

Abstract 219: Molecular and therapeutic characterization of a large-scale collection of metastatic colorectal cancer patient-derived xenografts and matched organoids for translational oncology

Abstract Strong evidence supports the use of patient-derived xenografts (PDXs) as faithful models reflecting patient outcomes. Since not all research can support the use of animals, exploiting patient-derived in vitro models such as organoids (also know as tumoroids) is essential. However, current biobanks of metastatic colorectal cancer (mCRC) tumoroids have important limitations, such as small sample size and a lack of systematic in vivo validation with paired xenografts, which decreases the translational value of tumoroid-based pipelines. Here we describe XENTURION, a unique open-science resource of XENografts and Tumoroids for Research In ONcology that encompasses 129 sibling pairs of mCRC PDXs and PDX-derived tumoroids (PDXTs) with accompanying molecular and therapeutic characterization.The vast majority of XENTURION models were analyzed for mutations (targeted next-generation sequencing of 116 relevant CRC genes), gene copy number architecture (DNA shallow sequencing) and global transcriptomics (RNAseq) and benchmarked against large patient datasets (TCGA and MSK-IMPACT). Results showed that the PDX-PDXT collection proved to be representative of the genetic heterogeneity of mCRCs and displayed high genetic and transcriptional similarity between matched pairs. A PDXT-based population trial with the clinically approved anti-EGFR antibody cetuximab was performed in 116 PDXTs, revealing variable sensitivities that were consistent with clinical response biomarkers and with tumor growth changes in 79 matched PDXs. Cetuximab response profiles also recapitulated the outcome of EGFR knock-out by CRISPR-Casp9 technology in 13 representative PDXTs. Adaptive signals upregulated by EGFR blockade both in tumoroids and PDXs were computationally and functionally prioritized. Top candidates were screened in PDXTs and 3 surviving compounds were identified as actionable co-extinction targets with cetuximab in 12 PDXT models. Results were finally validated in PDXs.To our knowledge, this is the first large-scale study in which a systematic comparison of molecular and therapeutic profiles between PDXT-PDX pairs was attempted. As a publicly available resource, XENTURION will offer a knowledge base of disseminatable methods, resources and information to streamline preclinical studies and accelerate new treatments for patients with mCRC. Citation Format: Simonetta M. Leto, Elena Grassi, Marco Avolio, Valentina Vurchio, Francesca Cottino, Martina Ferri, Eugenia R. Zanella, Laura Di Blasio, Alberto Puliafito, Luca Primo, Andrea Bertotti, Livio Trusolino. Molecular and therapeutic characterization of a large-scale collection of metastatic colorectal cancer patient-derived xenografts and matched organoids for translational oncology [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 219.

Using Electronic Health Record Data to Determine the Safety of Aqueous Humor Liquid Biopsies for Molecular Analyses

PurposeKnowing the surgical safety of anterior chamber liquid biopsies will support the increased use of proteomics and other molecular analyses to better understand disease mechanisms and therapeutic responses in patients and clinical trials. Manual review of operative notes from different surgeons and procedures in electronic health records (EHR) is cumbersome, but free-text software tools could facilitate efficient searches. DesignRetrospective case series. Participants1,418 aqueous humor (AH) liquid biopsies from patients undergoing intraocular surgery. MethodsFree-text EHR searches were performed using the Stanford Research Repository (STARR) cohort discovery tool to identify complications associated with anterior chamber paracentesis and subsequent endophthalmitis. Complications of the surgery unrelated to the biopsy were not reviewed. Main Outcome MeasuresBiopsy associated intraoperative complications and endophthalmitis. Results1,418 AH liquid biopsies were performed by 17 experienced surgeons. EHR free-text searches were 100% error-free for surgical complications, >99% for endophthalmitis (<1% false positive), and >93.6% for anesthesia type, requiring manual review for only a limited number of cases. More than 85% of cases were performed under local anesthesia without ocular muscle akinesia. Although the most common indication was cataract (50.1%), other diagnoses included glaucoma, diabetic retinopathy, uveitis, age-related macular degeneration, endophthalmitis, retinitis pigmentosa, and uveal melanoma. A 50-100μL sample was collected in all cases using either a 30-gauge needle or a blunt cannula via a paracentesis. The median follow-up was more than seven months. There was only one minor complication (0.07%) identified: a case of a small tear in Descemet’s membrane without long-term sequelae. No other complications occurred, including other corneal injuries, lens or iris trauma, hyphema, or suprachoroidal hemorrhage. There was no case of postoperative endophthalmitis. ConclusionsAnterior chamber liquid biopsy during intraocular surgery is a safe procedure and may be considered for large-scale collection of AH samples for molecular analyses. Free-text EHR searches are an efficient approach to reviewing intraoperative procedures.

Small Disturbance Stability Analysis of Onshore Wind Power All-DC Power Generation System Based on Impedance Method

The Onshore Wind Power All-DC Generation System (OWDCG) is designed to integrate with renewable energy sources by modifying the grid structure. This adaptation supports the grid infrastructure and addresses the challenges of large-scale wind power AC collection and harmonic resonance during transmission. Crucially, small disturbance stability parameters are essential for ensuring the system’s stable operation. Unlike conventional power systems, the OWDCG exhibits strong coupling between subsystems, accentuating the small disturbance stability issue due to the dynamic nature of its converter control system. The impedance method facilitates the decomposition of such systems into subsystems, offering insights into the destabilization mechanism through the lens of negative impedance contribution. This approach is conducive to conducting small disturbance stabilization analyses. To tackle this issue, the initial step involves deriving the input and output equivalent impedance models of the subsystem, considering the topological structure, control features, and operational dynamics of the OWDCG. Subsequently, the impact of circuit and control parameters on the system’s impedance characteristics and small-disturbance stability is examined through Bode diagrams and Nyquist curves. This analysis identifies critical parameters for small-disturbance stability, guiding the stable operation and parameter optimization of the OWDCG. The analysis highlights that the main control strategies for stability are the Modular Multilevel Converter (MMC) DC voltage control and the inner-loop current control gain. Validation of the theoretical findings is achieved through simulation results using PSCAD/EMTDC.

Development of phenotyping algorithms for hypertensive disorders of pregnancy (HDP) and their application in more than 22,000 pregnant women

Recently, many phenotyping algorithms for high-throughput cohort identification have been developed. Prospective genome cohort studies are critical resources for precision medicine, but there are many hurdles in the precise cohort identification. Consequently, it is important to develop phenotyping algorithms for cohort data collection. Hypertensive disorders of pregnancy (HDP) is a leading cause of maternal morbidity and mortality. In this study, we developed, applied, and validated rule-based phenotyping algorithms of HDP. Two phenotyping algorithms, algorithms 1 and 2, were developed according to American and Japanese guidelines, and applied into 22,452 pregnant women in the Birth and Three-Generation Cohort Study of the Tohoku Medical Megabank project. To precise cohort identification, we analyzed both structured data (e.g., laboratory and physiological tests) and unstructured clinical notes. The identified subtypes of HDP were validated against reference standards. Algorithms 1 and 2 identified 7.93% and 8.08% of the subjects as having HDP, respectively, along with their HDP subtypes. Our algorithms were high performing with high positive predictive values (0.96 and 0.90 for algorithms 1 and 2, respectively). Overcoming the hurdle of precise cohort identification from large-scale cohort data collection, we achieved both developed and implemented phenotyping algorithms, and precisely identified HDP patients and their subtypes from large-scale cohort data collection.

Investigation of policy tools for energy efficiency improvement in public buildings in China—current situation, obstacles, and solutions

The energy consumption from the building sector accounts for more than 21% of the total in China, where the energy-use intensity of public buildings could reach 3 times that of residential buildings. Energy efficiency improvement (EEI) is considered to be one of the most rapid and cost-effective means for the building sector to achieve carbon neutrality. The Chinese government has carried out a series of policy tool exploration to promote the energy efficiency improvement efforts by public building owners since over a decade ago, but encountered various problems and obstacles. This paper investigated and compared the current status challenges, and problems in the implementation of EEI policy tools in China and developed countries through literature review, field investigations and interviews with experts and stakeholders, and finally also put forward some possible coping strategies for Chinese government. Results indicated that energy grading and labeling, energy benchmarking, carbon-emission trading and energy-consumption quota management were the main 4 EEI policy tools designed based on measured energy consumption data, where the first 3 ones had been widely used over the developed countries but the last one was unique to China. Difficulty in large-scale collection of building energy-consumption data, lack of scientific methods on building energy-use level evaluation, limited data application and feedback, undesirable association between stakeholders, etc., were the main obstacles for achieving the long-term effectiveness of the EEI policy tools. Cooperating with energy supply enterprises to collect data, establishing an effective energy grading system and a closed loop of policy implementation, exploring a new model of building carbon-emission trading and a differential pricing mechanism, combined with innovative financial supports, might be the solutions. The main contribution of this study is that a comprehensive investigation and analysis on the current development status, obstacles, and possible solutions of the public building EEI policy tools in China was carried out, which is expected to provide a reference for the government of China and even the world.

Automatic label curation from large-scale text corpus

Abstract The topic modeling technique extracts themes based on their probabilistic measurements from any large-scale text collection. Even though topic modeling pulls out the most important phrases that describe latent themes in text collections, a suitable label has yet to be found. Interpreting the topics extracted from a text corpus and identifying a suitable label automatically reduces the cognitive load for the analyst. Extractive methods are used typically to select a label from a given candidate set, based on probability metrics for each candidate set. Some of the existing approaches use phrases, words, and images to generate labels using frequency counts of different words in the text. The paper proposes a method to generate labels automatically to represent each topic based on a labeling strategy to filter candidate labels and then apply sequence-to-sequence labelers. The objective of the method is to get a meaningful label for the result of the Latent Dirichlet Allocation algorithm. The BERTScore metric is used to evaluate the effectiveness of the proposed method. The proposed method generates good interpretative labels as compared to baseline models for topic words or terms automatically. The comparison with the label generated through ChatGPT API shows the quality of the generated label with the experiment performed on Four Datasets NIPS, Kindle, PUBMED, and CORD-19.

The adaptive community-response (ACR) method for collecting misinformation on social media

Social media can be a major accelerator of the spread of misinformation, thereby potentially compromising both individual well-being and social cohesion. Despite significant recent advances, the study of online misinformation is a relatively young field facing several (methodological) challenges. In this regard, the detection of online misinformation has proven difficult, as online large-scale data streams require (semi-)automated, highly specific and therefore sophisticated methods to separate posts containing misinformation from irrelevant posts. In the present paper, we introduce the adaptive community-response (ACR) method, an unsupervised technique for the large-scale collection of misinformation on Twitter (now known as ’X’). The ACR method is based on previous findings showing that Twitter users occasionally reply to misinformation with fact-checking by referring to specific fact-checking sites (crowdsourced fact-checking). In a first step, we captured such misinforming but fact-checked tweets. These tweets were used in a second step to extract specific linguistic features (keywords), enabling us to collect also those misinforming tweets that were not fact-checked at all as a third step. We initially present a mathematical framework of our method, followed by an explicit algorithmic implementation. We then evaluate ACR on the basis of a comprehensive dataset consisting of >25\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$>25$$\\end{document} million tweets, belonging to >300\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$>300$$\\end{document} misinforming stories. Our evaluation shows that ACR is a useful extension to the methods pool of the field, enabling researchers to collect online misinformation more comprehensively. Text similarity measures clearly indicated correspondence between the claims of false stories and the ACR tweets, even though ACR performance was heterogeneously distributed across the stories. A baseline comparison to the fact-checked tweets showed that the ACR method can detect story-related tweets to a comparable degree, while being sensitive to different types of tweets: Fact-checked tweets tend to be driven by high outreach (as indicated by a high number of retweets), whereas the sensitivity of the ACR method extends to tweets exhibiting lower outreach. Taken together, ACR’s capacity as a valuable methodological contribution to the field is based on (i) the adoption of prior, pioneering research in the field, (ii) a well-formalized mathematical framework and (iii) an empirical foundation via a comprehensive set of indicators.

AHP DB: a reference database of proteins in the human aqueous humor.

The aqueous humor (AH) is a low-viscosity biofluid that continuously circulates from the posterior chamber to the anterior chamber of the eye. Recent advances in high-resolution mass-spectrometry workflows have facilitated the study of proteomic content in small-volume biofluids like AH, highlighting the potential clinical implications of the AH proteome. Nevertheless, in-depth investigations into the role of AH proteins in ocular diseases have encountered challenges due to limited accessibility to these workflows, difficulties in large-scale AH sample collection and the absence of a reference AH proteomic database. In response to these obstacles, and to promote further research on the involvement of AH proteins in ocular physiology and pathology, we have developed the web-based Aqueous Humor Proteomics Database (AHP DB). The current version of AHP DB contains proteomic data from 307 human AH samples, which were analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The database offers comprehensive information on 1683 proteins identified in the AH samples. Furthermore, relevant clinical data are provided for each analyzed sample. Researchers also have the option to download these datasets individually for offline use, rendering it a valuable resource for the scientific community. Database URL: https://ahp.augusta.edu/.

A reproducible and generalizable software workflow for analysis of large-scale neuroimaging data collections using BIDS Apps

Abstract Neuroimaging research faces a crisis of reproducibility. With massive sample sizes and greater data complexity, this problem becomes more acute. Software that operates on imaging data defined using the Brain Imaging Data Structure (BIDS)—the BIDS App—has provided a substantial advance. However, even using BIDS Apps, a full audit trail of data processing is a necessary prerequisite for fully reproducible research. Obtaining a faithful record of the audit trail is challenging—especially for large datasets. Recently, the FAIRly big framework was introduced as a way to facilitate reproducible processing of large-scale data by leveraging DataLad—a version control system for data management. However, the current implementation of this framework was more of a proof of concept, and could not be immediately reused by other investigators for different use cases. Here, we introduce the BIDS App Bootstrap (BABS), a user-friendly and generalizable Python package for reproducible image processing at scale. BABS facilitates the reproducible application of BIDS Apps to large-scale datasets. Leveraging DataLad and the FAIRly big framework, BABS tracks the full audit trail of data processing in a scalable way by automatically preparing all scripts necessary for data processing and version tracking on high performance computing (HPC) systems. Currently, BABS supports jobs submissions and audits on Sun Grid Engine (SGE) and Slurm HPCs with a parsimonious set of programs. To demonstrate its scalability, we applied BABS to data from the Healthy Brain Network (HBN; n = 2,565). Taken together, BABS allows reproducible and scalable image processing and is broadly extensible via an open-source development model.

Analysis of the Change of Accounting Firms by Listed Companies- -Take New Asia Manufacturing Process (Zhejiang) Co., Ltd. as an Example

In the current market economic environment, the phenomenon of frequent replacement of accounting firms in China has attracted wide attention inside and outside the industry. On the one hand, this trend reflects the change of market competition and corporate business needs. On the other hand, it may also affect investors 'decisions and the public's trust in listed companies. Therefore, it is obviously necessary to study the motivation behind the replacement of accounting firms in listed companies. This paper aims to explore the motivation of Chinese listed companies to change accounting firms and their potential influence. Firstly, through large-scale data collection and analysis, this paper described the basic status of changing accounting firms by listed companies in China in recent years.Then, we discussed the various possible reasons for listed companies to replace their accounting firms. After in-depth research and analysis, we find that although the replacement of accounting firms can enhance the credibility of the financial reports. Therefore, this paper put forward a series of regulatory countermeasures. The main significance of this study lies in the in-depth discussion of the phenomenon of listed companies changing accounting firms, which provides more detailed market data and rich empirical analysis framework for future research.

Theorizing the concept of social tie using frames

In classical Social Network Analysis (SNA), what counted as a “social tie” was fixed by available data collection methods. The emergence of large-scale unobtrusive data collection techniques has sparked renewed interest in the very idea of what counts as a “social tie.” Importantly, there has been an acknowledgment that the core issues raised by these developments are primarily conceptual. As a result, there is renewed interest in developing a scientifically grounded characterization of what is arguably the most central concept in social network analysis. This paper contributes to this conceptual effort. I rely on a technique of conceptual representation borrowed from cognitive psychology and cognitive linguistics in which frames for concepts are represented as directed graphs linking attributes to values. I show how the frame representation helps clarify the sort of claims that network theories make (e.g., imposing restrictions on attributes and values), how it helps specify both intra and inter-conceptual relations, how it illuminates seldom noted inter-theoretical commonalities and contrasts, and how it helps avoid common conceptual pitfalls.

Low-Cost Biosensor Technologies for Rapid Detection of COVID-19 and Future Pandemics.

Many systems have been designed for the detection of SARS-CoV-2, which is the virus that causes COVID-19. SARS-CoV-2 is readily transmitted, resulting in the rapid spread of disease in human populations. Frequent testing at the point of care (POC) is a key aspect for controlling outbreaks caused by SARS-CoV-2 and other emerging pathogens, as the early identification of infected individuals can then be followed by appropriate measures of isolation or treatment, maximizing the chances of recovery and preventing infectious spread. Diagnostic tools used for high-frequency testing should be inexpensive, provide a rapid diagnostic response without sophisticated equipment, and be amenable to manufacturing on a large scale. The application of these devices should enable large-scale data collection, help control viral transmission, and prevent disease propagation. Here we review functional nanomaterial-based optical and electrochemical biosensors for accessible POC testing for COVID-19. These biosensors incorporate nanomaterials coupled with paper-based analytical devices and other inexpensive substrates, traditional lateral flow technology (antigen and antibody immunoassays), and innovative biosensing methods. We critically discuss the advantages and disadvantages of nanobiosensor-based approaches compared to widely used technologies such as PCR, ELISA, and LAMP. Moreover, we delineate the main technological, (bio)chemical, translational, and regulatory challenges associated with developing functional and reliable biosensors, which have prevented their translation into the clinic. Finally, we highlight how nanobiosensors, given their unique advantages over existing diagnostic tests, may help in future pandemics.

Liquid Biopsy Proteomics in Ophthalmology.

Minimally invasive liquid biopsies from the eye capture locally enriched fluids that contain thousands of proteins from highly specialized ocular cell types, presenting a promising alternative to solid tissue biopsies. The advantages of liquid biopsies include sampling the eye without causing irreversible functional damage, potentially better reflecting tissue heterogeneity, collecting samples in an outpatient setting, monitoring therapeutic response with sequential sampling, and even allowing examination of disease mechanisms at the cell level in living humans, an approach that we refer to as TEMPO (Tracing Expression of Multiple Protein Origins). Liquid biopsy proteomics has the potential to transform molecular diagnostics and prognostics and to assess disease mechanisms and personalized therapeutic strategies in individual patients. This review addresses opportunities, challenges, and future directions of high-resolution liquid biopsy proteomics in ophthalmology, with particular emphasis on the large-scale collection of high-quality samples, cutting edge proteomics technology, and artificial intelligence-supported data analysis.

Bi-Objective Incentive Mechanism for Mobile Crowdsensing With Budget/Cost Constraint

In recent years, mobile crowdsensing (MCS) has been widely adopted as an efficient method for large-scale data collection. In MCS systems, insufficient participation and unstable data quality have become two crucial issues that prevent crowdsensing from further development. Thus designing a valid incentive mechanism is essentially significant. Most of the existing works on incentive mechanism design focus on single-objective optimization with various constraints. However, in the real-world crowdsensing, it is common that several objectives to be optimized exist. Furthermore, constraints on budget or cost are often seen in MCS systems as the feasibility of implementing incentive mechanism is indispensable. This paper studies a bi-objective optimization scenario of MCS to simultaneously optimize total value function and coverage function with budget/cost constraint through a set of problem transformations. Then a budget- or cost-feasible bi-objective incentive mechanism is further proposed to solve the aforementioned bi-objective optimization problem through the combination of binary search and greedy heuristic solution under budget or cost constraint, respectively. Through both rigorous theoretical analysis and extensive simulations, the obtained results demonstrate that the mechanisms achieve computation efficiency, individual rationality, truthfulness, and budget or cost feasibility, while one mechanism obtains an approximation.

The Puzzle of Large-Scale Digital Collections: Have We Reached an Inflection Point?

The Puzzle of Large-Scale Digital Collections: Have We Reached an Inflection Point?

Topology Optimization of Large-Scale Offshore Wind Power Collection System Based on Graph Genetic DMST

Topology Optimization of Large-Scale Offshore Wind Power Collection System Based on Graph Genetic DMST

OldVisOnline: Curating a Dataset of Historical Visualizations.

With the increasing adoption of digitization, more and more historical visualizations created hundreds of years ago are accessible in digital libraries online. It provides a unique opportunity for visualization and history research. Meanwhile, there is no large-scale digital collection dedicated to historical visualizations. The visualizations are scattered in various collections, which hinders retrieval. In this study, we curate the first large-scale dataset dedicated to historical visualizations. Our dataset comprises 13K historical visualization images with corresponding processed metadata from seven digital libraries. In curating the dataset, we propose a workflow to scrape and process heterogeneous metadata. We develop a semi-automatic labeling approach to distinguish visualizations from other artifacts. Our dataset can be accessed with OldVisOnline, a system we have built to browse and label historical visualizations. We discuss our vision of usage scenarios and research opportunities with our dataset, such as textual criticism for historical visualizations. Drawing upon our experience, we summarize recommendations for future efforts to improve our dataset.

Sampling strategies for genotyping common bean (Phaseolus vulgaris L.) Genebank accessions with DArTseq: a comparison of single plants, multiple plants, and DNA pools.

Genotyping large-scale gene bank collections requires an appropriate sampling strategy to represent the diversity within and between accessions. A panel of 44 common bean (Phaseolus vulgaris L.) landraces from the Alliance Bioversity and The Alliance of Bioversity International and the International Center for Tropical Agriculture (CIAT) gene bank was genotyped with DArTseq using three sampling strategies: a single plant per accession, 25 individual plants per accession jointly analyzed after genotyping (in silico-pool), and by pooling tissue from 25 individual plants per accession (seq-pool). Sampling strategies were compared to assess the technical aspects of the samples, the marker information content, and the genetic composition of the panel. The seq-pool strategy resulted in more consistent DNA libraries for quality and call rate, although with fewer polymorphic markers (6,142 single-nucleotide polymorphisms) than the in silico-pool (14,074) or the single plant sets (6,555). Estimates of allele frequencies by seq-pool and in silico-pool genotyping were consistent, but the results suggest that the difference between pools depends on population heterogeneity. Principal coordinate analysis, hierarchical clustering, and the estimation of admixture coefficients derived from a single plant, in silico-pool, and seq-pool successfully identified the well-known structure of Andean and Mesoamerican gene pools of P. vulgaris across all datasets. In conclusion, seq-pool proved to be a viable approach for characterizing common bean germplasm compared to genotyping individual plants separately by balancing genotyping effort and costs. This study provides insights and serves as a valuable guide for gene bank researchers embarking on genotyping initiatives to characterize their collections. It aids curators in effectively managing the collections and facilitates marker-trait association studies, enabling the identification of candidate markers for key traits.