Large Arena Research Articles

Post-trauma behavioral phenotype predicts the degree of vulnerability to fear relapse after extinction in male rats

Current treatments for trauma-related disorders remain ineffective for many patients.1,2 Fear extinction deficiency is a prominent feature of these diseases,3 and many behavioral treatments rely on extinction training.4,5 However, in many patients, therapy is followed by a relapse of symptoms, and the underpinnings of such interindividual variations in vulnerability to relapse remain unknown.6-8 Here, we modeled interindividual differences in post-therapy fear relapse with an ethologically relevant trauma recovery paradigm. After fear conditioning, male rats underwent fear extinction while foraging in a large enriched arena, permitting the expression of a wide spectrum of behaviors. An automated multidimensional behavioral assessment revealed that post-conditioning fear response profiles clustered into two groups: some animals expressed fear by freezing more, whereas others darted more, as if fleeing from danger. Remarkably, the tendency of an animal to dart or to freeze after CS presentation during the first extinction session was, respectively, associated with stronger or weaker fear renewal. Moreover, genome-wide transcriptional profiling revealed that these groups differentially regulated specific sets of genes, some of which were previously implicated in anxiety and trauma-related disorders. Our results suggest that post-trauma behavioral phenotypes and the associated gene expression landscapes can serve as markers of fear relapse susceptibility and thus may be instrumental for future development of more effective treatments for psychiatric patients.

The Sustainability Mindset Principles: A Guide to Developing a Mindset for a Better World

The Sustainability Mindset Principles: A Guide to Developing a Mindset for a Better World

Perspective: Narrative Storyliving in Virtual Reality Design

The development and relative affordability of Virtual Reality in recent years have provided opportunities to experience representations of both concrete and abstract situations; from nuclear engineering to particle physics, art galleries to three-dimensional prehistoric paintings, person-to-person communication to artificial agent collaboration, and 360-degree journalism to animated movies. Yet, it still remains challenging for participants to create personal narratives within a virtual world beyond that structured by its original designers. Setting aside technological considerations, we attribute this limitation largely to a restricted conceptualization of time and space that is fixed to present events, emotions and experiences. Consequently, Virtual Reality scenarios, as immersive and plausible as they might be, are nonetheless prone to a thin and static view of the (virtual) world where growth and experiential learning are not always possible or privileged. In this Perspective we propose a recasting of Virtual Reality that combines novelistic storytelling in the physical world with “narrative storyliving” as a mechanism for meaning-making within and across large dialogic arenas. This involves us drawing on ideas from the Russian philosopher and theorist, Mikhail Bakhtin, relating to the literary artistic chronotope. Ultimately, we intend to advance the discourse about what Virtual Reality is at present, and where it could go as seen through a critical literary lens.

Metabolic cost of flight and aerobic efficiency in the rose chafer, Protaetia cuprea (Cetoniinae).

Rose chafer beetles (Protetia cuprea) are pollinators as well as agricultural pests, flying between flowers and trees while foraging for pollen and fruits. Calculating the energy they expend on flying during foraging activity faces the challenge of measuring the metabolic rate (MR) of free-flying insects in an open space. We overcame this challenge by using the bolus injection of 13 C Na-bicarbonate technique to measure their metabolic energy expenditure while flying in a large flight arena. Concurrently, we tracked the insects with high-speed cameras to extract their flight trajectory, from which we calculated the mechanical power invested in flying for each flight bout. We found that the chemical (metabolic) energy input converted to mechanical flight energy output at a mean efficiency of 10.4%±5.2%, with a trend of increased efficiency in larger conspecifics (efficiency scaled with body mass to the power of 1.4). The transition in the summer from a diet of pollen to that of fruits may affect the energy budget available for foraging. Starved P. cuprea, feeding on apples ad libitum, increased their body mass by an average of 6% in 2h. According to our calculations, such a meal can power a 630-m flight (assuming a carbohydrate assimilation efficiency of 90%). Pollen, with a low water and carbohydrate content but rich in proteins and lipids, has a higher caloric content and should assimilate differently when converting food to flight fuel. The high cost of aerial locomotion is inherent to the foraging behavior of rose chafers, explaining their short flight bouts followed by prolonged feeding activity.

Behavioural analysis of swarming mosquitoes reveals high hearing sensitivity in Anopheles coluzzii.

Mosquitoes of many species mate in station-keeping swarms. Mating chases ensue as soon as a male detects the flight tones of a female with his auditory organs. Previous studies of hearing thresholds have mainly used electrophysiological methods that prevent the mosquito from flying naturally. The main aim of this study was to quantify behaviourally the sound level threshold at which males can hear females. Free-flying male Anopheles coluzzii were released in a large arena (∼2 m high×2 m×1 m) with a conspicuous object on the ground that stimulated swarming behaviour. Males were exposed to a range of natural and synthetic played-back sounds of female flight. We monitored the responses of males and their distance to the speaker by recording changes in their wingbeat frequency and angular speed. We show that the mean male behavioural threshold of particle velocity hearing lies between 13 and 20 dB sound particle velocity level (SVL) (95% confidence interval). A conservative estimate of 20 dB SVL (i.e. <0.5 µms-1 particle velocity) is 12-26 dB lower than most of the published electrophysiological measurements from the Johnston's organ. In addition, we suggest that (1) the first harmonic of a female's flight sound is sufficient for males to detect her presence, (2) males respond with a greater amplitude to single-female sounds than to the sound of a group of females and (3) the response of males to the playback of the flight sound of a live female is the same as to that of a recorded sound of constant frequency and amplitude.

Incrementing non-matching- but not matching-to-sample is rapidly learned in an automated version of the odor span task.

The odor span task (OST) is frequently used to assess memory capacity in rodents. Odor stimuli are presented in a large arena and choices of session-novel odors produce food reward. The procedure can be described as an incrementing non-matching-to-sample contingency because on each trial one new stimulus is presented along with one or more previously presented (non-reinforced) comparison odors. An automated version of this task has recently been developed in which odors are presented with an olfactometer in an operant chamber using a successive conditional discrimination procedure. The present study compared the acquisition of matching- vs. non-matching-to-sample versions of the task with six rats tested under each procedure. All six rats trained on the non-matching variation showed rapid acquisition of the discrimination with high rates of responding to odor stimuli when they were session-novel and low rates of responding to subsequent presentations of those odors. However, only three of the six rats trained on the matching variation met acquisition criteria, and two of the three that did acquire the task required extensive training to do so. These results support findings from the OST that rats can differentiate between stimuli that are session-novel and those previously encountered, but alsothat a matching contingency is more difficult to learn than a non-matching arrangement. These findings parallel differences observed between acquisition of simple matching- and non-matching-to-sample tasks, but accounts such as novelty preference or the oddity preference effect may not be sufficient to explain the present results.

The Status and Future of Arabic Use amid Colonial Languages in the Arab World in Times of Globalization and Advanced Technology: A Political and Sociolinguistic Approach

This paper studies the languages and cultures that are commonly present in individual and collective practices of the Arab world, to understand the extent to which the identity and the linguistic approach in the region are affected by the historical and geographical context. It analyzes the status of Arabic and its future amid colonial languages, such as English and French, in the Arab world, specifically Morocco and Lebanon as case studies, in times of globalization and advanced technology. In a large and diversified arena, such as the Arab world, there is often a strong commitment and devotion to languages, such as Arabic, French, and English. Accordingly, this study further examines how Arabic can strengthen its practice and protect its status in an environment dominated by colonial languages. In this regard, the future of the Arabic language in the current ever-changing sociolinguistic context is subject to questioning and concerns for its official forthcoming evolution. Globalization, internet, social networks, digital technologies, and fast communication are no longer enabling linguistic authorities to provide a clear and accurate vision on the future of this language. Thus, addressing the question of languages in the future pushes researchers to consider and analyze the several linguistic strategies and policies implemented by the concerned authorities who are building and promoting an overall representation within and outside their original area.

Schoolyard Affordances for Physical Activity: A Pilot Study in 6 Nordic–Baltic Countries

Environmental settings influence children’s and adolescents’ physical activity (PA) in neighborhoods and schoolyards. This study aimed to explore the main characteristics of schoolyards in six Nordic–Baltic countries, to document how those facilities provide affordances for PA in 7–18 year–old schoolchildren, and how the schoolyard meets children’s preferences. One schoolyard was studied in each included country: Iceland, Norway, Finland, Lithuania, Latvia, and Estonia. The affordances, facilities, and equipment for PA in schoolyards were identified through orthophoto maps and standard registration forms. Children’s preferences were collected through group interviews at each participating school. A common design of schoolyards across countries indicated mostly flat topography with sparse vegetation and green areas dominated by large traditional sport arenas such as a football field, areas suitable for ball games, and track and field activities. Green spaces and varied topography were more prominent in Nordic countries. Across nationalities, the responses from pupils regarding the schoolyard were similar: they liked it though they wished for more variety of activities to do during recess. National regulations/recommendations for schoolyard design differed across the countries, being more restricted to sport fields and sport-related activities in Latvia and Lithuania, while in Nordic countries, the recommendations focused more on versatile schoolyard design.

Sexual Incentive Motivation and Copulatory Behavior in Male Rats Treated With the Adrenergic α2-Adrenoceptor Agonists Tasipimidine and Fadolmidine: Implications for Treatment of Premature Ejaculation

Premature ejaculation is the most common sexual dysfunction in young men, and it often leads to reduced relationship satisfaction and quality of life. To determine the role of central and peripheral α2-adrenoceptors in the control of ejaculation and sexual incentive motivation in rats. Sexual incentive motivation was studied in a large arena in which a male subject could choose between approaching and remaining close to a sexually receptive female or another male. Sexual behavior was studied in standard observation cages in which a male was allowed to freely interact with a receptive female for 30 minutes. Two highly selective agonists at the α2-adrenoceptors, tasipimidine and fadolmidine, were administered before the tests. Low peripheral doses of fadolmidine have been reported to have effects mainly outside of the central nervous system, whereas at large doses also the central effects are evident. The time spent close to the receptive female in relation to the time spent with the male and measures of ambulatory activity were obtained from the test for sexual incentive motivation, while the habitual parameters of sexual behavior were recorded with the copulation test. Tasipimidine prolonged ejaculation latency and the interintromission interval at the dose of 200 µg/kg when data from fast-ejaculating rats were used. No other sexual parameter was modified. A dose of 100 µg/kg was ineffective. There was no consistent effect on sexual incentive motivation, although modest sedation was observed. Fadolmidine, a drug that does not easily penetrate the blood-brain barrier, had no effect on sexual incentive motivation at any of the doses used (3, 30, and 100 µg/kg). The largest dose had clear sedative effects. The lower doses had no systematic effect on sexual behavior, not even when only fast or very fast ejaculating males were analyzed. The findings are relevant to the search for treatments for premature ejaculation that are specific enough to selectively delay ejaculation. The procedures used here are standard in the field and yield the most reliable data. Whether the effects observed in male rats are directly transferrable to men can only be determined through clinical studies. The observation that drugs acting at central but not peripheral α2-adrenoceptors prolong ejaculation latency without affecting any other parameter of sexual behavior or sexual incentive motivation suggests that this kind of drug may be suitable for treating premature ejaculation.

Pupils Participation in a Technology-Rich Environment: A Study of Pupil Perspective on Learners’ and Teachers’ Roles

It has been argued that participation as involvement as well as taking part in decision making is a fundamental part of learning. The first large arena for experiencing this for many young people is the school. This paper investigates how 16 pupils at grade eight view their own and their teacher’s role when it comes to facilitating participation. The pupils are interviewed the semester after a long researcher led participation project and the interviews are analyzed using thematic analysis. The analysis identifies four different types of participation from the pupils’ responses. These four types can be connected to passive and active participation as well as participation as involvement and participation as influence.

Characterization of exploratory patterns and hippocampal–prefrontal network oscillations during the emergence of free exploration

During free exploration, the emergence of patterned and sequential behavioral responses to an unknown environment reflects exploration traits and adaptation. However, the behavioral dynamics and neural substrates underlying the exploratory behavior remain poorly understood. We developed computational tools to quantify the exploratory behavior and performed in vivo electrophysiological recordings in a large arena in which mice made sequential excursions into unknown territory. Occupancy entropy was calculated to characterize the cumulative and moment-to-moment behavioral dynamics in explored and unexplored territories. Local field potential analysis revealed that the theta activity in the dorsal hippocampus (dHPC) was highly correlated with the occupancy entropy. Individual dHPC and prefrontal cortex (PFC) oscillatory activities could classify various aspects of free exploration. Initiation of exploration was accompanied by a coordinated decrease and increase in theta activity in PFC and dHPC, respectively. Our results indicate that dHPC and PFC work synergistically in shaping free exploration by modulating exploratory traits during emergence and visits to an unknown environment.

Phonotactic responses of Brown Rats (Rattus norvegicus) to begging calls of Starling nestlings (Sturnus vulgaris)

Foraging behavior of Brown Rats (Rattus norvegicus) is commonly thought to be guided by olfactory cues. Here we tested the hypothesis that foraging Brown Rats eavesdrop on bird vocalizations to locate prey. We recorded calls of nestling Starlings (Sturnus vulgaris) with microphones sensitive in the sonic and ultrasonic range, respectively, and compiled clear begging calls into a single sound file (2-min long) which included the entire recorded sound range (0–100 kHz; Audio File 1). Sound analyses revealed a fundamental two-tone modulated sound centered around 3 kHz, a first and second harmonic at, respectively, 9 and 16 kHz, and bands of ultrasonic frequency components at 20, 25, and 37 kHz. We subjected Audio File 1 to low- and highpass-filtering, thereby producing a sonic-range only file (< 20 kHz; Audio File 2), and an ultrasonic-range only file (20–100 kHz; Audio File 3). In binary-choice large arena bioassays, we then tested the behavioral responses of single Brown Rats to paired trap boxes each fitted with a speaker, one of which emitting a white noise control and the other playing back Audio File 1 (Exp. 1), Audio File 2 (Exp. 2), or Audio File 3. In each of experiments 1–3, female and male rats (i) significantly more often entered first the trap box broadcasting an Audio File, and (ii) spent significantly more time in the arena quadrant with an Audio File trap box. Our data support the conclusion that foraging Brown Rats, as opportunistic omnivores, exploit begging calls of nestling Starlings, and possibly other birds, as cues to obtain a proteinaceous meal.

Combined Effect of Entomopathogens against Thrips tabaci Lindeman (Thysanoptera: Thripidae): Laboratory, Greenhouse and Field Trials.

Simple SummaryOnion thrips Thrips tabaci Lindeman (Thysanoptera: Thripidae) is one of the most damaging insect pests of onions, Allium cepa L., which is an economically important agricultural crop cultivated worldwide. In the present study, the combination of Heterorhabditis bacteriophora (VS strain), Steinernema feltiae (SN strain) with Beauveria bassiana (WG-11) and Metarhizium anisopliae (WG-02) caused greater mortality compared to lone application of each agent, with prominent additive interaction observed. The pre-pupal stage was found to be the most susceptible stage compared to pupae and late second instar larvae of T. tabaci. In potted plant bioassay under greenhouse conditions, again, combination of pathogens produced significantly fewer adults compared to lone application of each pathogen. In field trials, combination of pathogens showed lower numbers of larvae and adults compared to lone application of each pathogen, and an increase in plant growth was also observed among the treated group compared to the control group.Onion thrips, Thrips tabaci Lindeman (Thysanoptera: Thripidae) is one of the most damaging insect pests of onions, Allium cepa L., which is an economically important agricultural crop cultivated worldwide. In this study, the combined application of entomopathogenic nematodes with entomopathogenic fungi against different soil dwelling stages of T. tabaci was evaluated. The nematodes included Heterorhabditis bacteriophora (VS strain) and Steinernema feltiae (SN strain), and fungi included Beauveria bassiana (WG-11) and Metarhizium anisopliae (WG-02); all four paired combinations (nematode + fungus) were included. In a small cup bioassay, only the combined application of H. bacteriophora and B. bassiana (WG-11) caused a synergistic interaction against pre-pupae, while all other combinations were compatible in an additive manner against pupae and late second instars. In a larger arena, a potted soil bioassay, again, combined applications of both pathogens produced greater mortality compared to single applications of each pathogen; all the combinations exhibited additive interactions, with the highest mortality observed in pre-pupae, followed by pupae and late second instar larvae using H. bacteriophora and B. bassiana (WG-11). Additionally, in the potted plant bioassay, lower adult emergence was observed from treated groups compared to control groups. Under field conditions, lower numbers of adults and larvae were found in treated groups relative to controls. Overall, the pre-pupal stage was more susceptible to the pathogen treatments, followed by pupae and late second instar larvae, and also combined applications of both pathogens suppressed the adult population. Combined application of entomopathogenic nematodes and fungi could be used for integrated pest management (IPM) of T. tabaci in onion production systems.

High-Throughput Screening of Psychotropic Compounds: Impacts on Swimming Behaviours in Artemia franciscana.

Animal behaviour is becoming increasingly popular as an endpoint in ecotoxicology due to its increased sensitivity and speed compared to traditional endpoints. However, the widespread use of animal behaviours in environmental risk assessment is currently hindered by a lack of optimisation and standardisation of behavioural assays for model species. In this study, assays to assess swimming speed were developed for a model crustacean species, the brine shrimp Artemia franciscana. Preliminary works were performed to determine optimal arena size for this species, and weather lux used in the experiments had an impact on the animals phototactic response. Swimming speed was significantly lower in the smallest arena, whilst no difference was observed between the two larger arenas, suggesting that the small arena was limiting swimming ability. No significant difference was observed in attraction to light between high and low light intensities. Arena size had a significant impact on phototaxis behaviours. Large arenas resulted in animals spending more time in the light side of the arena compared to medium and small, irrespective of light intensity. The swimming speed assay was then used to expose specimens to a range of psychotropic compounds with varying modes of action. Results indicate that swimming speed provides a valid measure of the impacts of behaviour modulating compounds on A. franciscana. The psychotropic compounds tested varied in their impacts on animal behaviour. Fluoxetine resulted in increased swimming speed as has been found in other crustacean species, whilst oxazepam, venlafaxine and amitriptyline had no significant impacts on the behaviours measured. The results from this study suggest a simple, fast, high throughput assay for A. franciscana and gains insight on the impacts of a range of psychotropic compounds on the swimming behaviours of a model crustacean species used in ecotoxicology studies.

The interaction between a Q-fuzzy normal subgroup and a Q-fuzzy characteristic subgroup

Subsequently after the insertion of fuzzy sets by L. A. Zadeh indefinite number of researchers interpreted on the generalisation of the notion of fuzzy sets. Fuzzy sets span large arenas of research in engineering, medical sciences, social sciences, graph theory, etc. In this paper we introduce the concept of a Q-Fuzzy normal subgroup and Q-fuzzy characteristic subgroup and with the help of a Klein-4 Group we study the interaction between them.

Wirelessly-Powered Cage Designs for Supporting Long-Term Experiments on Small Freely Behaving Animals in a Large Experimental Arena

In modern implantable medical devices (IMDs), wireless power transmission (WPT) between inside and outside of the animal body is essential to power the IMD. Unlike conventional WPT, which transmits the wireless power only between fixed Tx and Rx coils, the wirelessly-powered cage system can wirelessly power the IMD implanted in a small animal subject while the animal freely moves inside the cage during the experiment. A few wirelessly-powered cage systems have been developed to either directly power the IMD or recharge batteries during the experiment. Since these systems adapted different power carrier frequencies, coil configurations, subject tracking techniques, and wireless powered area, it is important for designers to select suitable wirelessly-powered cage designs, considering the practical limitations in wirelessly powering the IMD, such as power transfer efficiency (PTE), power delivered to load (PDL), closed-loop power control (CLPC), scalability, spatial/angular misalignment, near-field data telemetry, and safety issues against various perturbations during the longitudinal animal experiment. In this article, we review the trend of state-of-the-art wirelessly-powered cage designs and practical considerations of relevant technologies for various IMD applications.

Selective activation of the right hippocampus during navigation by spatial cues in domestic chicks (Gallus gallus)

In different vertebrate species, hippocampus plays a crucial role for spatial orientation. However, even though cognitive lateralization is widespread in the animal kingdom, the lateralization of this hippocampal function has been poorly studied. The aim of the present study was to investigate the lateralization of hippocampal activation in domestic chicks, during spatial navigation in relation to free-standing objects. Two groups of chicks were trained to find food in one of the feeders located in a large circular arena. Chicks of one group solved the task using the relational spatial information provided by free-standing objects present in the arena, while the other group used the local appearance of the baited feeder as a beacon. The immediate early gene product c-Fos was employed to map neural activation of hippocampus and medial striatum of both hemispheres. Chicks that used spatial cues for navigation showed higher activation of the right hippocampus compared to chicks that oriented by local features and compared to the left hippocampus. Such differences between the two groups were not present in the left hippocampus or in the medial striatum. Relational spatial information seems thus to be selectively processed by the right hippocampus in domestic chicks. The results are discussed in light of existing evidence of hippocampal lateralization of spatial processing in chicks, with particular attention to the contrasting evidence found in pigeons.

Utilization of displacement ventilation and on-site measurement of thermal environment in an ice arena

Thermal environment in ice arenas is significantly different from that in common commercial buildings. Strict dry and cold environment is required near the ice for sports standards, while the spectators in view stands would prefer a comfortable environment. A zoning air conditioning scheme with two alternative displacement air supply systems is proposed for an ice arena, and the on-site measurement is carried out in the present research. Ground displacement ventilation is used in the ice area, while seat displacement ventilation is used for spectators. Results show that the temperature and humidity ratio near the ice vary at the range of 6.8–9.6 °C, 2.9–3.1 g/kg respectively in any conditions. In the ice area, the controlling height of air parameters is about 1.25 m with obvious stratification in vertical direction. At the same time, the operating temperature is maintained at about 20 °C in the spectator area within thermal comfort range. It is hoped to provide a new idea for the air conditioning terminal in ice arenas, especially for the large space arenas.

CRISPR/Cas9 mediated disruption of the white gene leads to pigmentation deficiency and copulation failure in Drosophila suzukii

The Spotted-wing Drosophila (Drosophila suzukii) is a devastating invasive pest of fruit crops. In D. melanogaster, the white (w) gene was associated with pigmentation and mating behavior, which are also important aspects to understand the invasion biology as well as to develop control strategies for D. suzukii. Here, we show that the generation of D. suzukii white-eyed mutants by CRISPR/Cas9 mutagenesis of the w gene resulted in the complete failure of copulation when w− males were individually paired with w− females in small circular arenas (diameter 0.7 cm) for 24 h. Further analysis showed that the mating defect was associated with w− males and could not be rectified by two years of inbreeding by crossing sibling w− females with w+ males, dim red illumination, male–female sexual training, changing to large arenas (diameter 3.5 cm), or different sex ratios. Profound pigmentation deficiency was detected in the compound eyes, ocelli, Malpighian tubules and testis sheaths in the w− flies. Specifically, testis imaging showed that w− males failed to deposit any pigments into pigment cells of the testis sheath, and produced smaller sperms and less seminal fluid compared to those from wildtype males. Together these observations suggest that the w gene plays an essential role in the regulation of sexual behavior and reproduction in D. suzukii. The similarities and differences in w gene function between D. suzukii and D. melanogaster in the context of pigmentation and mating behavior are discussed.

Successive incrementing non-matching-to-samples in rats: An automated version of the odor span task.

The odor span task is a procedure frequently used to study remembering of multiple stimuli in rodents. A large arena is used and odor stimuli are presented using scented cups. Selection of each odor is reinforced when first presented, but not on subsequent presentations; correct selections depend on remembering which stimuli were previously presented. The use of an arena setting with manual stimulus presentation makes the odor span task labor-intensive and limits experimental control; thus, an automated version of the task would be of value. The present study used an operant chamber equipped with an olfactometer and trained rats using successive conditional discrimination procedures under an incrementing non-matching-to-samples contingency. High rates of responding developed to odor stimuli when they were session-novel with low rates of responding to subsequent presentations of that odor. Additional experiments assessed variations of the procedure to determine the role of the frequency of odor presentation and the retention interval separating sample and comparison. Discrimination was impaired with long retention intervals suggesting the importance of this variable. These findings confirmed that rats differentiate between stimuli that are session-novel and those previously encountered and support the use of an automated procedure as an alternative to the odor span task.