The QB50 mission is a satellite constellation designed to carry out measurements at between 200–380 km altitude in the ionosphere. The multi-needle Langmuir probe (m-NLP) instrument has been mounted on board eleven QB50 satellites in order to characterize ambient plasma. The distinct feature of this instrument is its capability of measuring the plasma density at high spatial resolution without the need to know the electron temperature or the spacecraft potential. While the instrument has been deployed on many sounding rockets, the QB50 satellites offer the opportunity to demonstrate the operation of the instrument in low-earth orbit (LEO). This paper provides a brief review of the m-NLP instrument specifically designed for the QB50 mission and the case studies of the instrument’s performance on board the Ex-Alta 1 and Hoopoe satellites. The system has also been functionally verified in a plasma chamber at the European Space Research and Technology Center (ESTEC). Although the QB50 mission’s scientific goals have not been reached yet and some uncertainties still remain, there are some optimistic in-orbit preliminary results which could be helpful for the system improvement in future campaigns. Particularly, the electron emitter as part of the m-NLP science unit has demonstrated its capability in the plasma chamber and in orbit to mitigate spacecraft charging effects.