A new endangered species habitat evaluation method based on spatial variables and behavior was developed and applied in a recent development permit application process in south Florida. It utilizes aspects of habitat quality, landscape arrangement, movement tendencies, and human disturbance to generate an empirical approach to estimating functional equivalent units of ideal Florida panther (Pumaconcolorcoryi) habitat. The model can be used by permitting agencies to ensure an objective and consistent landscape approach to panther conservation throughout south Florida, and it may be useful in much of its former range (the southeastern coastal plain of the United States). It is intended for use on lands that are proposed for development as well as for property that could be acquired or otherwise managed to accommodate panther population growth and colonization.