• Day/night seasonal and annual changes and trends (2001- 2018) in land surface temperature and surface urban heat island intensity (SUHII) for Indian cities (Lucknow, Kolkata and Pune). • Nighttime LST is a better indicator to study the urban heating effect due to its sensitivity towards urbanization. • Positive trends in mean annual daytime (0.003 to 0.059 °C/year) and nighttime (0.030 to 0.078 °C/year) LST are observed in all cities, except in Pune where urban cooling occurs during daytime. • Nighttime SUHII is positive for all cities with mean annual SUHII ranging from 1.34 to 2.07 °C. Understanding the patterns and possible causes of urban heat islands (UHI) effect due to urbanisation-induced anthropogenic activities is an important area in urban climatic research. Few studies on long-term thermal behaviour of Indian cities are available in the literature. Towards this, the present study investigates the day/night seasonal and annual changes and trends in land surface temperature (LST) and surface urban heat island intensity (SUHII) during the last two decades for three Indian cities (Lucknow, Kolkata and Pune) with distinct urban, physiographic and climatic setting. Moderate Resolution Imaging Spectroradiometer (MODIS) LST data products acquired during daytime and nighttime from 2001 to 2018 are processed and analysed for this purpose. MODIS-based aerosol optical depth (AOD) and normalised difference vegetation index (NDVI) products are used to understand the plausible reasons of change in LST and SUHII patterns. The urban (contiguous built-up) and non-urban (surrounding rural) areas are delineated by applying city clustering algorithm on MODIS Land Cover datasets of 500 m spatial resolution. Mann-Kendall and Seasonal-Kendall tests along with Theil-Sen estimator are used for trend analysis. The findings show that the diurnal temperature range (DTR) has decreased from 2001 to 2018 due to higher increase in nighttime LST as compared to daytime. Nighttime LST is a better indicator to study the urban heating effect due to its sensitivity towards urbanisation. Positive trends in mean annual daytime (0.003 to 0.059 °C/year) and nighttime (0.030 to 0.078 °C/year) LST are observed in all cities, except in Pune where urban cooling occurs during daytime. Increase in aerosol loading (with consequent decrease in air temperature and surface insolation) and vegetative cover over time are attributed as the primary reasons of urban cooling effect in Pune during daytime. Seasonal analysis indicate high warming rates during monsoon and summer seasons, particularly during nighttime (0.062 to 0.1 °C/year). Nighttime SUHII is positive for all cities with mean annual SUHII ranging from 1.34 to 2.07 °C. Statistically significant ( p <0.01) increasing trends are observed in mean annual SUHII (0.009 °C/year to 0.022 °C/year) for Kolkata and Pune. Decreasing trend in SUHII is observed over Lucknow due to higher rate of increase in LST in non-urban area as compared to urban area. The study highlights that geographical, physiographic and climatic setting along with anthropogenic processes in urban and its surrounding non-urban areas are critical towards understanding the causative factors in exacerbating the phenomenon of urban heating.